Abstract：
:Regulation of glucose-induced oscillations in intracellular Ca2+ concentration ([Ca2+]i) was investigated by using a novel technique, electroporation from an electrolyte-filled capillary, to deliver energy metabolites to the intracellular compartment of mouse islets. Intracellular application of ATP resulted in a nifedipine-sensitive increase in [Ca2+]i, consistent with a KATP-channel dependent mechanism of Ca2+ influx. [Ca2+]i in islets exposed to 10 mM glucose oscillated with a period of approximately 3 min, often superimposed with faster oscillations. Electroporation of ATP blocked all types of oscillations and elevated [Ca2+]i while delivery of ADP had no effect on oscillations. Intracellular delivery of glucose-6-phosphate or fructose-1,6-bisphosphate tended to transform slow oscillations to fast oscillations. These results demonstrate that modulation of ATP concentrations and glycolytic flux are important in development of slow oscillations.
journal_name
Life Scijournal_title
Life sciencesauthors
Dahlgren GM,Nolkrantz K,Kennedy RTdoi
10.1016/j.lfs.2005.05.053subject
Has Abstractpub_date
2005-10-21 00:00:00pages
2986-97issue
23eissn
0024-3205issn
1879-0631pii
S0024-3205(05)00559-Xjournal_volume
77pub_type
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