Abstract：
:Three forms of alkaline phosphatase have been isolated from different sections of the small intestine: F3 180 kDa from the duodenum; F2 150 kDa principally jejunal; F1 120 kDa the only ileal form. Their catalytic properties have been compared as well as the electrophoretic properties the dimer and monomer of their phosphorylated intermediates. Pi was a competitive inhibitor of F1 and F3, whereas glycerophosphate was competitive inhibitor only of F3. Pi was a non competitive inhibitor of F2 and of a mixture F1 plus F3. Heating the phosphorylated enzyme preparations led to their dissociation into the phosphorylated monomers: F1 and F3 appear to be homodimers 65 kDa and 90 kDa peptides respectively whilst F2 seems to be a dimer formed from one of each monomer. F1 was phosphorylated faster but less intensively than F3. F2 was strongly phosphorylated over a long time-course and its 65 kDa monomer fraction was phosphorylated more strongly for longer than that from F1.
journal_name
Life Scijournal_title
Life sciencesauthors
Tardivel S,Razanamaniraka L,Porembska Z,Crouzoulon G,Fournier P,Dupuis Ydoi
10.1016/0024-3205(88)90355-4subject
Has Abstractpub_date
1988-01-01 00:00:00pages
2059-65issue
25eissn
0024-3205issn
1879-0631pii
0024-3205(88)90355-4journal_volume
43pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:This study was designed to determine whether the increase in atriopeptin secretion induced by an intravenous infusion of norepinephrine is mediated directly by adrenergic receptor stimulation or indirectly by the associated increase in atrial pressure. Norepinephrine was infused at 0.5 microgram.kg-1.min-1 for 30 min ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90046-t
更新日期：1990-01-01 00:00:00
abstract：:A simple and sensitive method using high performance liquid chromatography with fluorometric detection has been developed for the identification and quantitation of the endogeneous tryptamine metabolites, indoleacetic acid (IAA) and tryptophol (TOL) in the normal mouse brain. The limits of sensitivity are 5pg for both...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90744-0
更新日期：1983-11-14 00:00:00
abstract：:In 6-[18F]fluoro-L-dopa (Fdopa)/positron emission tomography (PET) studies, carbidopa pretreatment increases the Fdopa bioavailability to the brain and enhances the intensity of striatal PET images. Different PET research teams have used various carbidopa doses and routes of administration in non-human primate studies...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00147-x
更新日期：1995-01-01 00:00:00
abstract：AIMS:Nicotine is a risk factor for various diseases, including osteoporosis, oral cancer, and periodontal disease. Numerous studies have elucidated the effects of nicotine on cell proliferation and differentiation. The purpose of this study was to determine the effects of nicotine on the proliferation and osteoblast di...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.10.019
更新日期：2012-01-16 00:00:00
abstract：AIMS:Excessive production of nitric oxide (NO) and reactive oxygen species (ROS) in sepsis modulates different cell functions. Since the sepsis severity is associated with the degree of platelet activation, we decided to investigate the role of systemic generation of NO and ROS in modulating the platelet adhesion of li...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.09.004
更新日期：2011-11-21 00:00:00
abstract：:Semi-purified dog kidney Na+,K+-ATPase cross-linked with ovalbumin was used in batch-wise affinity chromatography for the detection of endogenous Na+,K+-ATPase inhibitor in human plasma and urine. Ammonium acetate 1 M washed off the endogenous inhibitor from the immobilized enzyme. The inhibitory activity of the eluat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90562-4
更新日期：1985-05-06 00:00:00
abstract：:Involvement of T-type voltage dependent Ca2+ channels (VDCCs) on morphine antinociception, in the development of tolerance and dependence to morphine, and naloxone-precipitated abstinence syndrome in morphine dependent mice was examined by using mibefradil, a T-type VDCCs blocker. Mice were rendered tolerant and depen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01736-8
更新日期：2002-06-28 00:00:00
abstract：:In recent years, serum S100B has been used as a secondary endpoint in some clinical trials, in which serum S100B has successfully indicated the benefits or harm done by the tested agents. Compared to clinical stroke studies, few experimental stroke studies report using serum S100B as a surrogate marker for estimating ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.06.031
更新日期：2007-08-02 00:00:00
abstract：:The effect of the repeated or continuous administration of an analog of GH releasing factor (GH-RF), D-Tyr-1, D-Ala-2, Nle-27, GH-RF(1-29)-NH2 (DBO-29), on the subsequent response to this peptide was investigated in pentobarbital-anesthetized male rats. A sc administration of this analog induced a greater and more pro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90759-4
更新日期：1987-06-22 00:00:00
abstract：AIMS:Ganoderma lucidum, a traditional Chinese medicine, is well known as a modulator of functions of the immune system as well as an anti-tumour agent. However, its active compounds and their molecular mechanisms of action are not well established. GLIS, a proteoglycan isolated from the fruiting body of G. lucidum, sti...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.09.026
更新日期：2010-11-20 00:00:00
abstract：AIMS:Intestinal alkaline phosphatase (IAP) is an intestinal brush border enzyme that is shown to function as a gut mucosal defense factor, but its defensive mechanism remains unclear. The aims of this study were to evaluate the effect of IAP on intestinal epithelial cells and macrophages, and on chronic colitis in inte...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.02.003
更新日期：2014-04-01 00:00:00
abstract：:In this study we compared the antioxidant properties of five different extracts of different composition obtained from Achyrocline satureioides' inflorescences (Compositae), a widely used Brazilian folk medicinal herb. All of the extracts presented significant antioxidant potential identified by TRAP assay, which incr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.073
更新日期：2004-04-23 00:00:00
abstract：AIMS:Stroke-prone spontaneously hypertensive rats (SHRSP) show significantly lower body weight than normotensive Wistar-Kyoto rats (WKY). Our hypotheses are as follows: weight loss of the skeletal muscle is related to hypertension-related diseases, and muscle hypotrophy is useful as a therapeutic target for hypertensio...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116919
更新日期：2019-11-15 00:00:00
abstract：:The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]cis methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90602-6
更新日期：1982-03-08 00:00:00
abstract：:A single intravenous injection of alloxan in mice induced hyperglycemia in a dose dependent fashion. This diabetogenic action of alloxan was prevented by a single intraperitoneal injection of the alpha 2-adrenergic agonists, i.e. oxymetazoline, clonidine or epinephrine 40 min prior to the injection of alloxan. The alp...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90301-6
更新日期：1983-02-14 00:00:00
abstract：:Several different vasodilator substances can be released by vascular endothelium in response to mechanical stimuli and vasoactive agents. The purpose of this study was to determine whether there is a male-female difference in the relative contributions of nitric oxide (NO) and endothelium-derived hyperpolarizing facto...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01851-9
更新日期：2002-08-23 00:00:00
abstract：:This study examined whether there is increased production of oxygen free radicals during chronic renal failure. Rats subjected to 3/4 nephrectomy and sham operated controls were killed after 3 weeks. Lipid extracts of plasma and renal tissue were examined by HPLC and kidney specimens were also analyzed by EPR spectros...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90548-4
更新日期：1992-01-01 00:00:00
abstract：:11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) inhibitors have been shown to treat type 2 diabetes (T2D). Since gossypol is an 11β-HSD1 inhibitor, the objective of the present study was to treat T2D and T2D-related liver fibrosis in rat model using low-dose gossypol. T2D was induced by feeding with high fat diet plus i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.02.044
更新日期：2016-03-15 00:00:00
abstract：:Despite the considerable number of published studies in the field of S-nitrosothiols (RSNO), the determination of these compounds in biological samples still represents an analytical challenge, due to several technical obstacles and often long sample preparation procedures. Other problems derive from the intrinsic lab...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2010.10.024
更新日期：2011-01-17 00:00:00
abstract：:As a decrease in cholinergic neurons has been observed in Alzheimer's Disease (AD), therapeutic approaches to AD include inhibition of acetylcholinesterase to increase acetylcholine levels. Evidence suggests that acetylcholine release in the CNS is modulated by negative feedback via presynaptic M2 receptors, blockade ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00598-0
更新日期：1999-01-01 00:00:00
abstract：AIMS:Keratinocytes are the predominant cells in the epidermis, exerting their primary role of physical barrier through sophisticated differentiation process. In addition, keratinocytes contribute to the activation of innate immunity, providing the surveillant role against external pathogens. It has been known that chro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.05.023
更新日期：2016-06-15 00:00:00
abstract：:A highly purified rat urinary bladder smooth muscle cell culture was obtained by a modified enzymic isolation method, and the presence of functional muscarinic as well as beta-adrenergic receptors were subsequently determined. At 7-10 days of culture, cells became elongated and spindle-shaped showing a typical "hills ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01488-6
更新日期：2002-01-25 00:00:00
abstract：:Species-related specific differences in the pharmacological profile of vasopressin V1a receptors have been reported. Thus, the aim of the present study was to identify a vascular preparation of human origin expressing V1a receptors. Vasopressin was found to contract human gastric artery strips without endothelium with...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00635-2
更新日期：1997-01-01 00:00:00
abstract：:The bradykinin-induced sensitization of cutaneous nociceptors to heat was previously shown to be abolished by cyclooxygenase blockade suggesting that endogenous prostaglandins exerted a heat-sensitizing action. The present study aimed at investigating the effects of exogenous prostaglandin E(2) (PGE(2)) and I(2) (PGI(...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.10.001
更新日期：2007-12-14 00:00:00
abstract：:Vitamin E treatment was found to lower blood pressure, and increase membrane fluidity in rats. The objectives of this study were to investigate the effects of the antioxidant, vitamin E, on the blood pressure and erythrocyte membrane fluidity in spontaneously hypertensive (SHR) and normotensive (WKY) rats. Membrane fl...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00775-x
更新日期：2000-09-01 00:00:00
abstract：:Angiotensin (AII) and atrial natriuretic peptide (ANP) exert opposite effects on phosphorylation of a 17.6 kDa nuclear protein from bovine adrenal glomerulosa cells. The protein was separated by sodium dodecyl sulfate electrophoresis and blotted onto polyvinylidene difluoride, and the N-terminal sequence was obtained....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90465-4
更新日期：1990-01-01 00:00:00
abstract：:The anticonvulsant activity of U-54494A was studied in a 4-aminopyridine (4-AP) epilepsy model using extracellular recordings in in vitro hippocampal slices. Field potentials were evoked by stimulation of Schaffer collaterals, and recorded from the CA1 region of the hippocampus after infusion of 4-AP in the absence an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90381-x
更新日期：1992-01-01 00:00:00
abstract：:Because of the low selectivity of available ligands, pharmacological approaches to elucidate the functional difference among muscarinic acetylcholine receptor (mAChR) subtypes have been problematic. As an alternative approach, we have established a series of mutant mouse lines deficient in each mAChR subtype (mAChR KO...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2004.05.034
更新日期：2004-11-05 00:00:00
abstract：:The challenge of discovering new, urgently needed anti-TB drugs from natural sources requires a truly interdisciplinary research. Cutting-edge mycobacteriology and innovative natural products chemistry tools have to be developed and employed in tandem, in order to meet these demands. The present review provides cross-...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2005.09.004
更新日期：2005-12-22 00:00:00
abstract：AIMS:Lipid soluble vitamin E plays a role in several physiological mechanisms, however, the mechanism of this action is controversial. We investigated how tocopherol (α-tocopherol acid succinate) influences the effects of cyclooxygenase inhibitors (COXi) in the smooth muscles. MAIN METHODS:The contractility of the sam...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.11.048
更新日期：2018-01-01 00:00:00
