Abstract：
:Semi-purified dog kidney Na+,K+-ATPase cross-linked with ovalbumin was used in batch-wise affinity chromatography for the detection of endogenous Na+,K+-ATPase inhibitor in human plasma and urine. Ammonium acetate 1 M washed off the endogenous inhibitor from the immobilized enzyme. The inhibitory activity of the eluate from hypertensive plasma and urine was significantly higher (p less than 0.0025, n = 5 and p less than 0.005, n = 6 respectively) than that of normotensive. This latter was correlated with the ability of plasma from the same subjects to compete with ouabain binding to erythrocytes. Plasma and urine extracts inhibited the activity of Na+, K+-ATPase in a dose-dependent manner as ouabain does and were shown to contain 3 or 4 active compounds by high pressure liquid chromatography. The activity of some of these compounds was lost after peptidase treatment. These data support the heterogeneity of endogenous inhibitors of Na+,K+-ATPase activity in plasma and urine.
journal_name
Life Scijournal_title
Life sciencesauthors
Henning G,Cloix JF,Wainer IW,Pernollet MG,Devynck MA,Meyer Pdoi
10.1016/0024-3205(85)90562-4subject
Has Abstractpub_date
1985-05-06 00:00:00pages
1787-98issue
18eissn
0024-3205issn
1879-0631pii
0024-3205(85)90562-4journal_volume
36pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Cloned human MCF-7 breast tumor cells were prevented from proliferating when grown in charcoal-dextran stripped human female serum (CDFHS)-supplemented media (40% and 10%); this inhibition was maximally cancelled by estradiol-17, cisTamoxifen, and Metabolite E, whereas Tamoxifen, N-desmethylTamoxifen and Metabolite Y ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90510-7
更新日期：1985-07-29 00:00:00
abstract：:We describe here a nonpeptide neuropeptide Y Y1 receptor antagonist, 2,4-dioxo-1,5-bis(2-oxo-2-orthotolyl-ethyl)-3-[3-[3-([3-[3-(3-p iperidin-1-ylmethyl-phenoxy)-propylcarbamoyl]-propyl]-car bamoyloxymethyl)-phenyl]-ureido]-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diaz epine (Compound 1), which was previously synthesized as...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00357-9
更新日期：1998-01-01 00:00:00
abstract：:Thermodynamic analysis of pharmacologic data potentially offers an insight into the molecular events underlying drug-receptor interactions not obtainable by other techniques. Embodied in thermodynamics are the laws governing the interconvertibility of heat and work and, hence, it is a particularly apt framework for th...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(89)90182-3
更新日期：1989-01-01 00:00:00
abstract：AIMS:Endothelin-1 (ET-1) and endothelin-2 (ET-2; Trp(6)Leu(7)ET-1) are expressed by different cell types, but are considered to display identical pharmacological properties on endothelin receptors. We studied agonist-dependent aspects of endothelin(A) (ET(A))-receptor function and the importance of amino acids 6 and 7 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.02.011
更新日期：2012-10-15 00:00:00
abstract：:The present study was designed to investigate the effects of the intrahippocampal co-injection of beta-amyloid protein1-40 (Abeta(1-40)) with ibotenic acid (Ibo) on learning and memory in normal and aging model rats, and to explore the mechanism underlying the effects of the co-injection. The normal and aging rats wer...
journal_title：Life sciences
pub_type： 杂志文章,收录出版
doi：10.1016/j.lfs.2004.05.039
更新日期：2005-01-28 00:00:00
abstract：:We investigated the effect of social isolation stress on the formation of experimental liver metastasis resulted from intraportal vein (i.p.v.) injection of colon 26-L5 carcinoma cells in male Balb/c mice, and elucidated some of the underlying mechanism involving the effects of this stress on cellular immunity. Increa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00506-3
更新日期：2000-03-31 00:00:00
abstract：:Aim Determine changes in the expressions of the ion channel-TRPV1-and neuropeptides-NKA, NKB, calcitonin gene-related peptide (CGRP), and SP-in 14-, 21-, and 42-day-old rats after inhaling 1.5% and 2.6% sevoflurane. MAIN METHODS:A small in-house inhalation anesthesia chamber was designed to allow 14-, 21-, and 42-day...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117472
更新日期：2020-05-15 00:00:00
abstract：AIMS AND METHODS:We previously reported that angiotensin II (AngII) restores the vascular reactivity diminished by hemorrhagic shock. In this study, we investigated whether the beneficial effects of AngII are related to regulation of gap junctions (GJs) and connexin43 (Cx43), and the implication of MAPK signaling and m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.11.019
更新日期：2019-01-01 00:00:00
abstract：:Although both the incidence and the mortality rate of breast cancer is rising, there is no potent and practical option for the treatment of these patients, particularly in advanced stages. One of the most critical challenges for treatment is the presence of complicated and extensive tumor escape mechanisms in the tumo...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.117437
更新日期：2020-04-15 00:00:00
abstract：:In adult male Wistar rats submitted to a standardized noise stress, intravenous TRH induced a prolactin (PRL) secretory response. Prior IV naloxone administration not only lowered plasma PRL levels in those stressed rats but abolished also the stimulatory action of TRH. This effect was further studied by superfusion e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90756-9
更新日期：1987-06-22 00:00:00
abstract：:Insulin resistance (IR) impairs endothelium-mediated vasodilation in cerebral arteries as well as K+ channel function in vascular smooth muscle. Peripheral arteries also show an impaired endothelium-dependent vasodilation in IR and concomitantly show an enhanced contractile response to endothelin-1 (ET-1). However, th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.01.028
更新日期：2005-09-16 00:00:00
abstract：AIMS:Amiodarone (AMIO) is currently used in medical practice to reverse ventricular tachycardia. Here we determine the effects of AMIO in the electromechanical properties of isolated left ventricle myocyte (LVM) from mice and guinea pig and in a cellular model of Long QT Syndrome Type 3 (LQTS-3) using anemone neurotoxi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117814
更新日期：2020-08-15 00:00:00
abstract：:Insulin therapy remains the standard of care for achieving and maintaining adequate glycemic control, especially in hospitalized patients with critical and noncritical illnesses. Insulin therapy is more effective against elevated fasting glycaemia but less in the reduction of postprandial hyperglycaemia. It is associa...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2013.12.210
更新日期：2014-03-18 00:00:00
abstract：:Renal handling of the aminoglycoside antibiotics gentamicin and tobramycin were studied before and after one hour of constant intravenous infusions adjusted to maintain a concentration of 15 micrograms/mL. A solitary renal autotransplant model in four conscious volume replete 40 Kg sheep was used. This unique surgical...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90032-2
更新日期：1983-01-17 00:00:00
abstract：AIMS:microRNA-96 (miR-96) is considered as a tumor suppressor in multiple malignancies. Some studies have indicated that EZRIN could be regulated by the miR-96 in several kinds of cancer cells. However, the function of miR-96 in human osteosarcoma has not been investigated intensively so far. In this study, we mainly e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.04.012
更新日期：2018-06-15 00:00:00
abstract：AIMS:To determine whether increased N-acetyltransferase (NAT) activity might have a toxic effect during development and an influence on folate levels since previous work has shown that only low levels of exogenous NAT can be achieved in constitutionally transgenic mice (Cao et al. 2005). MAIN METHODS:A human NAT1 tet-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.11.008
更新日期：2010-01-16 00:00:00
abstract：:Calcium ionophores, such as the A23187, cause endothelium-dependent relaxation of arterial strips with intact endothelium, whereas the effect of the ionophore should result from the combination of a relaxation caused by the endothelium-dependent factors and of a contraction of the smooth muscles. In addition, the appl...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00646-9
更新日期：2000-06-23 00:00:00
abstract：:Positive inotropic and chronotropic responses of guinea-pig isolated left and right atria to sympathomimetic amines were examined at bath temperatures of 38, 30 or 25 degrees C. The concentration-response curves to isoproterenol and orciprenaline were displaced to the left by cooling, indicating hypothermia-induced su...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90074-2
更新日期：1984-09-03 00:00:00
abstract：:We have previously shown that matrix metalloproteinases (MMPs) play a role in osteoclastic bone resorption by facilitating migration of osteoclastic cells toward bone surface through matrices. Of MMPs identified so far, MMP-9 is likely the most important proteinase for the action, since osteoclasts express this enzyme...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.05.024
更新日期：2006-09-20 00:00:00
abstract：:The effect of calcium (Ca2+) on the adenylate cyclase activity and calmodulin level of cerebral cortex was determined in pentobarbital dependent rats and age matched controls. Female Sprague-Dawley rats were made dependent and maintained on pentobarbital by eating a mixture of pentobarbital and rat chow (350 mg pentob...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90625-3
更新日期：1985-07-08 00:00:00
abstract：:The beta adrenoceptor antagonist radioligand [3H] dihydroalprenolol (DHA) has been used to characterise beta adrenoceptors in membranes prepared from rat renal glomeruli. Association of the ligand was rapid and had reached equilibrium within 10 mins at 37 degrees C. Dissociation occurred in two distinct phases, a rapi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90715-4
更新日期：1983-07-04 00:00:00
abstract：:Involvement of T-type voltage dependent Ca2+ channels (VDCCs) on morphine antinociception, in the development of tolerance and dependence to morphine, and naloxone-precipitated abstinence syndrome in morphine dependent mice was examined by using mibefradil, a T-type VDCCs blocker. Mice were rendered tolerant and depen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01736-8
更新日期：2002-06-28 00:00:00
abstract：:Pancreatitis is an inflammatory disease of the pancreas characterized by acinar cell injury and is associated with the abnormal release of trypsin, which results in high mortality due to systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS). The inflammatory response, impaired a...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118641
更新日期：2021-01-01 00:00:00
abstract：:Angiotensin (AII) and atrial natriuretic peptide (ANP) exert opposite effects on phosphorylation of a 17.6 kDa nuclear protein from bovine adrenal glomerulosa cells. The protein was separated by sodium dodecyl sulfate electrophoresis and blotted onto polyvinylidene difluoride, and the N-terminal sequence was obtained....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90465-4
更新日期：1990-01-01 00:00:00
abstract：:The putative anxiolytic activity of succinic acid was examined in male mice by using a number of experimental paradigms of anxiety and compared with that of the known anxiolytic compound diazepam. Use of the elevated plus-maze test revealed that diazepam (1.0, 2.0 and 4.0 mg/kg, PO) or succinic acid (3.0 or 6.0 mg/kg,...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.06.017
更新日期：2003-11-07 00:00:00
abstract：:Skeletal muscle viability is crucially dependent on the tissue levels of its high energy phosphates. In this study we investigated the effect of the preservation medium Perfadex and illumination with Singlet Oxygen Energy (SOE). Singlet oxygen can be produced photochemically by energy transfer from an excited photosen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00176-0
更新日期：2003-04-25 00:00:00
abstract：:Patients with neurogenic orthostatic hypotension due to multiple system atrophy (MSA) or pure autonomic failure (PAF) excrete lower amounts of homovanillic acid (HVA) than do normal subjects. There is a highly significant correlation between the rates of excretion of HVA and norepinephrine metabolites. The regression ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90289-5
更新日期：1988-01-01 00:00:00
abstract：:The effects of different kinds of acute stress on collagen-induced whole blood platelet aggregation and fibrinolysis in relation to blood serotonergic measures were studied. In rats water-immersion restraint stress resulted in a shortening of euglobulin clot lysis time (ECLT), an increase in tissue plasminogen activat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00855-8
更新日期：1994-01-01 00:00:00
abstract：:The conversion of lyso-platelet activating factor (lyso-PAF) to PAF in cultured paranasal sinus mucosa obtained from normal human subjects was studied. The PAF concentration in the medium was determined after addition of lyso-PAF. PAF became detectable at 10 minutes after the addition of 10(-8)M lyso-PAF, and reached ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90483-j
更新日期：1993-01-01 00:00:00
abstract：:Leucogenenol a heterocyclic enolic thymothyroid hormone (MW 383) whose concentration in the serum regulates the rate at which already committed cells of the bone marrow develop into functional cells, was found to be associated in the thymus with a carrier protein. The carrier protein for leucogenenol is not precipitat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90245-5
更新日期：1984-07-30 00:00:00
