Abstract:
:Cloned human MCF-7 breast tumor cells were prevented from proliferating when grown in charcoal-dextran stripped human female serum (CDFHS)-supplemented media (40% and 10%); this inhibition was maximally cancelled by estradiol-17, cisTamoxifen, and Metabolite E, whereas Tamoxifen, N-desmethylTamoxifen and Metabolite Y only partially blocked the inhibitory effect of CDFHS. The efficiency of this reversing effect was estradiol-17 greater than Metabolite E greater than cisTAM greater than OHTAM greater than TAM = Metabolite Y. CDFHS at 2% allowed for near maximal cell yield; estradiol-17 at concentrations above 3 X 10(-10) M inhibited cell proliferation whereas at lower concentrations was ineffective. All the triphenylethylenes tested at 2% CDFHS were toxic above 3 X 10(-7) M; beyond these concentrations, these drugs did not significantly affect the cell yield. The proliferative properties of E2 and these triphenylethylenes do not directly correlate with their binding affinities to the intracellular estrophilins. Finally, the control of the proliferation of C7MCF7-173 cells appears to be affected by the interaction among a) estradiol-17 or the triphenylethylenes, b) a specific blood-borne inhibitor of the proliferation of estrogen-sensitive cells (estrocolyones), and c) an inhibitor "receptor"-like structure in these target cells.
journal_name
Life Scijournal_title
Life sciencesauthors
Sonnenschein C,Papendorp JT,Soto AMdoi
10.1016/0024-3205(85)90510-7subject
Has Abstractpub_date
1985-07-29 00:00:00pages
387-94issue
4eissn
0024-3205issn
1879-0631pii
0024-3205(85)90510-7journal_volume
37pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Benzamide riboside (BR) is a novel anticancer agent exhibiting pronounced activity against several human tumor cell lines via the inhibition of inosine 5'-monophosphate dehydrogenase (IMPDH), thereby restricting the biosynthesis of guanylates. Although it has been demonstrated that BR inhibits IMPDH and induces apopto...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.11.026
更新日期:2004-05-28 00:00:00
abstract::It has been reported that while bacteria and higher plants possess two different pathways for the biosynthesis of putrescine, via ornithine decarboxylase (ODC) and arginine decarboxylase (ADC); the fungi, like animals, only use the former pathway. We found that contrary to the earlier reports, two of the phytopathogen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90317-2
更新日期:1989-01-01 00:00:00
abstract::Since reduced levels of brain serotonin are known to cause behavioural abnormalities, to which diabetics are also prone, we investigated the effect, in rats, of chronic diabetes on brain serotonin concentration and on the numbers of 5-HT(1A) and 5-HT2 receptors in cerebral cortex and brainstem. Our data show that stre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00084-2
更新日期:1997-01-01 00:00:00
abstract:AIMS:Osteoblasts and adipocytes originate from common precursor cells. We examined the effects of bone morphogenetic protein-2 (BMP-2) on the molecular mechanisms governing the diametric actions of BMP-2 on simultaneous mature osteoblast and adipocyte differentiation in a clonal mesenchymal progenitor cell line, ROB-C2...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.12.011
更新日期:2009-02-27 00:00:00
abstract::Stimulation of the preganglionic trunk of the ipsilateral superior cervical sympathetic ganglion with square wave electrical pulses produced secretion from the main excretory duct of the rabbit lacrimal gland. The secretion was inhibited by propranolol (1 mg/kg) and metoprolol (10 mg/kg) but not by phenoxybenzamine (2...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90424-7
更新日期:1984-06-11 00:00:00
abstract::The aims of the current study were: 1) to determine if the epsilonPKC pseudosubstrate peptide (epsilonphi) (NH(2)-RKRQGAVRRRVHQVNG-COOH) could be used as an epsilonPKC-selective inhibitor in neonatal cardiac myocytes (NCMs) and 2) to determine if differences in the alpha and epsilonPKC autoinhibitory pseudosubstrate m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.11.016
更新日期:2004-05-07 00:00:00
abstract::Toll-like receptors (TLRs) play important roles in the pathogenesis of atherosclerosis. On the other hand, serum high sensitivity C-reactive protein (hsCRP) is known as an independent coronary risk factor, but cardiovascular events do occur even in low hsCRP levels. We investigated whether the TLR4 expression levels o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.08.027
更新日期:2006-12-03 00:00:00
abstract::In order to understand the mechanism of increasing body fat in perimenopausal and postmenopausal women, an ovariectomy-induced obesity model was used to study the role of leptin. In this investigation, a long-term study lasted for 13 weeks was conducted to monitoring the change of serum leptin level in rats after the ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00181-2
更新日期:1999-01-01 00:00:00
abstract::Acute post-ischemic cardiac failure was studied in isolated rat hearts. Xylitol, glutamine, aspartic acid and glycine were added during reperfusion, resulting in no inotropic effect. Concanavalin A had a moderate inotropic effect. When concanavalin A added with xylitol, glutamine, aspartic acid and glycine, a rapid re...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90705-8
更新日期:1993-01-01 00:00:00
abstract::Synthetic human pancreatic Growth Hormone-Releasing Factor (hpGRF) elevated the plasma concentration of growth hormone (GH) in young and adult domestic fowl. This in vivo effect of hpGRF appeared to be largely similar for both the 32 amino-acid (hpGRF 1-32) or 40 amino-acid (hpGRF 1-40) polypeptide, although the effec...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90083-3
更新日期:1984-03-19 00:00:00
abstract::Linear sucrose density gradient centrifugation of a crude synaptosomal-mitochondrial preparation of rat striatum was performed at 82,500g for 7.5, 15 and 30 min and 1, 4 and 20 h. After centrifugation various marker enzyme activities were measured throughout the gradients, viz. tyrosine hydroxylase (TH) and DOPA decar...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90248-5
更新日期:1983-08-15 00:00:00
abstract::This review analyses the knowledge about the incidence of transient apnoeic spells, induced by substances which activate vagal chemically sensitive afferents. It considers the specificity and expression of appropriate receptors, and relevant research on pontomedullary circuits contributing to a cessation of respiratio...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.12.021
更新日期:2019-01-15 00:00:00
abstract::Hydroxyethyl starch (HES) found in rat serum and spleen after single and daily administrations of 0.9 g/kg for 1 week was characterized by gas-liquid chromatography. There was very little difference in the degree of substitution (D.S.) and molar substitution (M.S.) of HES in serum samples obtained at 1 hour and 57 day...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90737-3
更新日期:1983-11-14 00:00:00
abstract::The objective of this study was to understand the mechanism of action of nitric oxide (NO) in the heart by determining whether nitric oxide (NO) released from sodium nitroprusside (SNP) induces p38 mitogen activated protein kinase (p38 MAPK) phosphorylation and whether this is mediated through a cyclic GMP (cGMP)/prot...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.06.022
更新日期:2007-08-02 00:00:00
abstract::To evaluate the relative contributions of the ovarian inhibin and estradiol-17 beta (E) on the regulation of FSH secretion, inhibin and E in ovarian venous plasma (OVP) and FSH and LH in peripheral plasma were simultaneously measured using superovulating rats with special reference to follicular maturation. By the tra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90445-5
更新日期:1986-07-14 00:00:00
abstract::Immunotherapy with immune checkpoint inhibitors, such as anti-PD-1 drugs, is an area in increasing development for its efficacy and advantages in the treatment of advanced metastatic melanoma. In fact, immunotherapy has been the target of several and recent studies in different types of cancer, namely in melanoma, a g...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.117093
更新日期:2020-01-01 00:00:00
abstract::The effects of eight catechin derivatives on vascular tone in rat thoracic aorta were examined. Catechin derivatives (10 microM) potentiated the contractile response to phenylephrine in endothelium-intact arteries. The potentiations produced by EGCg and EGC were almost absent in endothelium-denuded arteries and abolis...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02080-5
更新日期:2002-10-11 00:00:00
abstract::The novel guanidines N-(3,4-dimethoxy-2-chlorobenzylideneamino)-guanidine (ME 10092) and N-(3,4-dimethoxy-2-chlorobenzylideneamino)-N1-hydroxyguanidine (PR5) were recently reported to exhibit promising cardioprotective activities in myocardial ischaemia and reperfusion in rats. The current study investigated for the f...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.03.030
更新日期:2004-09-10 00:00:00
abstract::Adenylate cyclase activity in isolated rat liver plasma membranes was inhibited by NADH in a concentration-dependent manner. Half-maximal inhibition of adenylate cyclase was observed at 120 microM concentration of NADH. The effect of NADH was specific since adenylate cyclase activity was not altered by NAD+, NADP+, NA...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90177-d
更新日期:1991-01-01 00:00:00
abstract::Methotrexate was found to stimulate asparagine synthetase activity in vivo by approximately six-fold in rat liver. The maximum effect of methotrexate on hepatic asparagine synthetase activity was observed sixteen hours after intraperitoneal injection of the drug. Cycloheximide, like methotrexate, is a protein synthesi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90524-0
更新日期:1982-03-22 00:00:00
abstract::Icariin (ICA), a flavonol glycoside isolated from Epimedium, has been considered as a potential alternative therapy for ischemic stroke. However, the protective mechanisms of ICA on cerebral ischemia-reperfusion (I/R) are not fully illuminated yet. The effects of ICA on ER stress and inflammatory response which were i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117847
更新日期:2020-08-15 00:00:00
abstract::Ischemia-reperfusion of organs such as the kidney produces reactive oxygen and free radical species in tissues and leads to injury of intracellular molecules critical to cell homeostasis. Ischemia-reperfusion affects the NADPH-dependent monooxygenase system including P450 system, which is also a source of reactive oxy...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00604-2
更新日期:1997-01-01 00:00:00
abstract::The antiestrogen tamoxifen (TAM) is widely used as a drug against breast cancer and is currently being tested as a chemopreventive agent. However, a number of studies showed genotoxic and carcinogenic effects of TAM. These effects are thought to be related to oxygen radical overproduction which occurs during TAM metab...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00969-9
更新日期:2001-03-02 00:00:00
abstract::Net water, Na+, Cl- and HCO3- fluxes were measured in in vivo rabbit ileal loops, while mucus secretion was assessed by measuring the glycoprotein or total sialic acid secreted into the lumen, or by measuring the luminal fluid viscosity. Inoculating loops with cholera enterotoxin (CT) produced a sustained secretion of...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90220-5
更新日期:1983-02-21 00:00:00
abstract::Histidine-rich glycoprotein (HRG) is a serum protein with possible pluripotent activities. In this study, a method for the quantification of rabbit histidine-rich glycoprotein (rHRG) was developed based upon the high affinity binding profile of rHRG to nickel-nitrilotriacetic acid (Ni-NTA), an improved chelation agent...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00261-3
更新日期:2003-05-23 00:00:00
abstract::Cholinergic neurons in the laterodorsal (LDT) and the pedunculopontine (PPT) tegmental nuclei act to promote REM sleep (REMS). The predominantly glutamatergic neurons of the REMS-induction region of the medial pontine reticular formation are in turn activated by cholinergic cells, which results in the occurrence of to...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(99)00649-9
更新日期:2000-04-14 00:00:00
abstract::Recent evidence suggests that the Type 1 opiate receptor (in rat striatal patches) is a mobile receptor which is able to adopt a mu, delta, or kappa opiate receptor ligand selectivity pattern under appropriate conditions. In this paper, we have investigated such a possibility for Type 2 opiate receptors which are visu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90183-7
更新日期:1982-10-18 00:00:00
abstract::Convulsive seizures are a potential side effect of antidepressant drug treatment and can be produced by all classes of antidepressants. It is also known that some convulsant and anticonvulsant drug actions are mediated by the GABA-receptor chloride-ionophore complex. Drugs acting at this complex appear to induce convu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90107-5
更新日期:1988-01-01 00:00:00
abstract::The effect of wortmannin (WT), an inhibitor of myosin light chain kinase (MLCK) as well as PI3-kinase, on catecholamine (CA) secretion in the perfused rat adrenal medulla was studied. After a 35-min application of 10 microM WT, secretory response to repetitive stimulation with 30 mM extracellular K+ was reduced to 30%...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00842-0
更新日期:2000-10-13 00:00:00
abstract:AIMS:Ovarian cancer is the most leading cause of deaths among gynecologic malignancies, and Neuron-restrictive silencer factor (NRSF) can be upregulated or downregulated according to the type of tumor. However, the expression and function of NRSF in ovarian cancer is still unknown. MAIN METHODS:Expression of NRSF in n...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.10.070
更新日期:2018-12-15 00:00:00