Abstract:
:The objective of this study was to understand the mechanism of action of nitric oxide (NO) in the heart by determining whether nitric oxide (NO) released from sodium nitroprusside (SNP) induces p38 mitogen activated protein kinase (p38 MAPK) phosphorylation and whether this is mediated through a cyclic GMP (cGMP)/protein kinase G (PKG) pathway. p38 MAPK activation was examined by Western blotting of whole cell lysates of embryonic chick cardiomyocytes with antibodies specific to the native or phosphorylated forms of p38 MAPK. SNP, 1 mM, which released significant amounts of NO as determined by Griess reaction, induced p38 MAPK phosphorylation that was apparent within 10 min, was significantly (p<0.05) greater than control at 60 min and remained higher than initial levels up to the 4 h end point of the experiment. This could not be attributed to hydrogen peroxide release from SNP as catalase did not affect SNP-induced p38 MAPK phosphorylation. SB202190, a relatively selective inhibitor of p38 MAPK, mainly p38alpha MAPK, inhibited SNP-induced p38 MAPK phosphorylation. SNP-induced p38 MAPK phosphorylation was not altered by pre-treatment with the PKG inhibitor KT 5823 or by ODQ a potent and selective inhibitor of NO-sensitive guanylyl cyclase. p38 MAPK phosphorylation was not induced by the cell permeable cGMP analogue, 8-Br-cGMP. In summary, considering that new therapeutic strategies aimed at NO and p38 MAPK are being considered for myocardial injury and heart failure, these data demonstrate that SNP induces p38 MAPK phosphorylation through a pathway that is independent of NO-induced activation of cGMP/PKG pathways and suggest that non cGMP/PKG regulatory proteins leading to p38 MAPK phosphorylation merit further investigation to address this therapeutic target.
journal_name
Life Scijournal_title
Life sciencesauthors
Rabkin SW,Klassen SS,Tsang MYdoi
10.1016/j.lfs.2007.06.022subject
Has Abstractpub_date
2007-08-02 00:00:00pages
640-6issue
8eissn
0024-3205issn
1879-0631pii
S0024-3205(07)00477-8journal_volume
81pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::It is well known that the immune function can be compromised by stress. To investigate immune function in mice stressed by experimental restraint or unavoidable and opioid dependent stress, we evaluated the changes in total body weight and in organ weights (liver, spleen and thymus) of these animals, as well as the ph...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90600-v
更新日期:1990-01-01 00:00:00
abstract::Plasma 7B2-immunoreactivity (7B2-IR) concentrations in umbilical artery (UA), umbilical vein (UV) and maternal vein (MV) were measured by RIA at the time of obstetrical delivery at term. Plasma 7B2-IR concentrations (Mean +/- SEM) in UA (N = 12), UV (N = 16) and MV (N = 16) were 725 +/- 69, 699 +/- 64 and 116 +/- 4.5 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90744-2
更新日期:1987-10-19 00:00:00
abstract:AIMS:Perinatal hypoxic-ischemic insult has acute and long term deleterious effects on many organs including heart. Although tumor necrosis factor alpha (TNF-α) has been reported to increase soon after hypoxia, the inhibition of this mediator has not been documented. The aim of this study was to investigate the effects ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.03.036
更新日期:2012-06-06 00:00:00
abstract::We investigated the role of nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) in hemodynamic action of leptin. The effect of leptin (1 mg/kg i.p.) on systolic blood pressure (SBP) was examined in lean rats and in rats made obese by feeding highly palatable diet for either 1 or 3 months. Separate ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.12.041
更新日期:2006-05-30 00:00:00
abstract:AIMS:Doxorubicin is a widely used anthracycline derivative anticancer drug. Unfortunately, the clinical use of doxorubicin has the serious drawback of cardiotoxicity. In this study, we investigated whether baicalein, a bioflavonoid, can prevent doxorubicin-induced cardiotoxicity in vivo and we delineated the possible u...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.11.018
更新日期:2016-01-01 00:00:00
abstract::The effects of nicotine on normal physiological function are of increasing concern. Preliminary to studies on the effects of prenatal exposure to nicotine, we examined methods of administering nicotine to rats. Drinking water containing nicotine was not palatable to rats and was an unsatisfactory method in our hands, ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90020-8
更新日期:1987-04-27 00:00:00
abstract:AIMS:To investigate the effects of curcumin on alveolar epithelial injury in a rat model of chronic obstructive pulmonary disease (COPD) and its potential mechanism. MAIN METHODS:The rat COPD model was established by cigarette smoke exposure combined with intratracheal administration of lipopolysaccharide. Thirty-eigh...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.09.001
更新日期:2016-11-01 00:00:00
abstract::Animal studies suggest nicotine and cannabinoids may significantly enhance the therapeutic value of neuroleptics in motor disorders. This was recently demonstrated in humans by the finding that chewing nicotine gum produced striking relief from tics and other symptoms of Tourette syndrome not controlled by neuroleptic...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(89)90444-x
更新日期:1989-01-01 00:00:00
abstract::Although one of the major genes which cause type 1 (insulin-dependent) diabetes mellitus is located in the class II HLA region in humans, its precise location is still unknown. In order to investigate whether TAP (Transporter associated with Antigen Processing) and LMP (Low Molecular Weight Polypeptide) genes, which a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00713-6
更新日期:1994-01-01 00:00:00
abstract::The acute dose-dependent effects of epinephrine and cocaine on heart rate and coronary flow rate (CFR) were examined in isolated, perfused (Langendorff) rat hearts from animals: i) pretreated with daily cocaine injections (20 mg/kg/day) for 8 weeks; ii) after 2-day withdrawal from 8-week cocaine pretreatment; iii) veh...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90390-d
更新日期:1990-01-01 00:00:00
abstract::This study examined the effects of chronic treatment of a new phosphodiesterase type 5 inhibitor, DA-8159, on endothelial dysfunction in stroke-prone spontaneously hypertensive rats (SHR-SP). Six-week-old male SHR-SP were divided into 4 groups; vehicle control, DA-8159 1, 3, and 10 mg/kg/day. During a 32-week experime...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.06.042
更新日期:2006-02-09 00:00:00
abstract::Diazepam (0.4-4 mg/kg i.p.) reduced the spontaneous tonic activity in the electromyogram (EMG) recorded from the gastrocnemius-soleus muscle of spastic mutant Han-Wistar rats in a dose-dependent manner. The muscle relaxant effect of diazepam was antagonized by the benzodiazepine antagonists Ro 15-1788 (5 mg/kg i.p.), ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90160-7
更新日期:1984-10-01 00:00:00
abstract::Using a specific radioimmunoassay, we measured concentrations of plasma 7B2 (a novel pituitary polypeptide) immunoreactivity (7B2-IR) in normal human subjects, patients with chronic renal failure and those with liver cirrhosis. Mean (+/- SEM) values of plasma 7B2-IR in normal healthy men and women were 55.8 +/- 1.2 pg...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90685-0
更新日期:1987-08-24 00:00:00
abstract::Tricyclic antidepressant drugs bind to histamine receptors in rodent and other mammalian tissues. However, a readily accessible, normal human tissue for studies of these drugs has not been available. We showed that freshly isolated human leukocytes can be used for this purpose. Following isolation by dextran sedimenta...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90441-7
更新日期:1984-12-17 00:00:00
abstract::10(-4) M ouabain increases aldosterone production at 3.6 and 5.4 mM potassium concentrations, whereas at high potassium concentration (7 mM) even lower concentration of ouabain (10(-8) M) decreases it. As ouabain has been shown to be produced by the adrenal cortex, a local paracrine regulation of aldosterone productio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90466-g
更新日期:1993-01-01 00:00:00
abstract::Male Sprague-Dawley rats were given a single electroconvulsive shock (ECS) on alternate days and sacrificed 48 hrs after 1, 3, or 5 seizures. The content of TRH in hippocampus, pyriform cortex and amygdala was increased 2.5-fold, 5.4-fold and 4.3-fold respectively, 48 hrs. after 3 alternate-day electroconvulsive shock...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90116-x
更新日期:1985-01-28 00:00:00
abstract::To understand whether chronic inflammation alters the development of morphine tolerance, the tail-flick test was used to evaluate the analgesic effect of morphine (75 mg tablet, s.c.) in the arthritic rats at the day 9-12 after the inoculation with Freund's adjuvant. Spinal cord monoamines and amino acid neurotransmit...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00008-9
更新日期:1999-01-01 00:00:00
abstract::A novel beta-carboline with benzodiazepine-like properties has recently been synthesized. We compared the effect of the i.v. administration of this drug, ZK 93423, with diazepam on the activity of nigral pars reticulata neurons which are known to be very sensitive to the inhibitory effect produced by GABA-mimetics and...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90333-x
更新日期:1987-04-06 00:00:00
abstract::The present study was undertaken to evaluate the cardioprotective potential of Curcuma longa (Turmeric) in the ischemia-reperfusion (I/R) model of myocardial infarction (MI). Wistar rats were divided into three groups and received saline orally (sham, control I/R group) and Curcuma longa 100 mg/kg (CL-100 treated grou...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.02.032
更新日期:2004-08-20 00:00:00
abstract::To clarify the mechanism of meconium-induced cellular injury, we examined the effect of meconium on the alveolar macrophages (AM) and bronchial epithelial cells (AK-D). Meconium obtained from healthy newborns was added to culture medium of AM and/or AK-D, which were cultured for 1 hour. Superoxide anion production of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90026-4
更新日期:1994-01-01 00:00:00
abstract::Three cholecystokinin type B (CCKB) receptor antagonists were labelled with 11C and evaluated ex vivo in rat biodistribution studies. The CCKB antagonists were YF 476 and two other compounds of the basic 3-ureido-1,4-benzodiazepine class. Following tail-vein administration of [11C]-YF 476 exceedingly low levels of rad...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)01021-3
更新日期:2001-02-02 00:00:00
abstract:HEADING AIMS:Abdominal aortic aneurysm (AAA) is featured by the growth impediment and apoptosis surge of VSMCs (vascular smooth muscle cells). MicroRNAs (miRNAs) are suggested to affect cellular behaviors including cell growth and apoptosis. This study concentrated on unraveling the emerging role of miR-28-5p in abdomi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117533
更新日期:2020-07-15 00:00:00
abstract::Rats administered cocaine 10 mg/kg i.p. exhibited hyperlocomotion which occurred within 5 min post injection. Subsequently, rats were injected with 10 mg/kg cocaine and brain and serum concentrations of cocaine were measured at 5, 10, and 20 min post injection. Within this time frame, cocaine concentration in limbic b...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00339-4
更新日期:1994-01-01 00:00:00
abstract::Dopamine (DA), one of the major sources of reactive oxygen species (ROS), is implicated in neuronal death associated with Parkinson's disease (PD). Preconditioning with oxidative stress has been shown to provide cytoprotection similar to ischemic preconditioning (IPC), against cell apoptosis. In this study, using the ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.048
更新日期:2005-11-19 00:00:00
abstract:AIMS:We performed a systematic review and meta-analysis on the effect of HIV infection and antiretroviral therapy (ART) on carotid intima-media thickness (cIMT) to elucidate the role of HIV infection and ART. Also, an analysis on the role of ethnicity and gender on cIMT in HIV-infected populations was performed. MAIN ...
journal_title:Life sciences
pub_type: 杂志文章,meta分析
doi:10.1016/j.lfs.2019.116851
更新日期:2019-10-15 00:00:00
abstract::A radioreceptor assay using [3H]nitrendipine and rat cerebral cortical membranes, in conjunction with equilibrium dialysis, measures the unbound (free) level of nitrendipine in human sera. The sensitivity of the assay is 0.1-0.2 picomoles/ml and is linear from 4 X 10(-11) to 4 X 10(-9) M nitrendipine. Other dihydropyr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90690-2
更新日期:1983-10-31 00:00:00
abstract::Immunoreactive (ir) beta-endorphin (b-END) and dynorphin (DYN) in rat brain and pituitary were measured after food and water deprivation and from brains taken during either day or night. In other rats, eating and drinking were measured following lesions in the arcuate n. Ir-DYN levels are higher in hypothalamus and lo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90221-1
更新日期:1982-10-18 00:00:00
abstract:AIMS:Epigenetic and genetic alterations are crucial events in the onset and progression of human cancers including colorectal cancer (CRC). This work aims to probe the relevance of lysine demethylase 5B (KDM5B) to the progression of CRC and the possible molecules involved. MATERIALS AND METHODS:KDM5B expression in CRC...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118726
更新日期:2021-01-01 00:00:00
abstract::We examined the effects of repeated administration of the selective serotonin uptake inhibitor (SSRI) fluoxetine (Flx) (5, 10, or 15 mg/kg i.p., twice daily for 21 days) on brain and plasma concentrations of the parent drug and its active desmethyl metabolite, norfluoxetine (NFlx), in rats during the 21-day regimen as...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00821-3
更新日期:1994-01-01 00:00:00
abstract::6-mercaptopurine (6-MP) has been used clinically for 40 years to maintain remission in patients with acute lymphoblastic leukemia (ALL). However, central nervous system (CNS) relapses frequently occur in patients with ALL who continuously receive anticancer drugs, including 6-MP, during remission maintenance therapy. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00637-2
更新日期:2000-01-01 00:00:00