Abstract：
:Tricyclic antidepressant drugs bind to histamine receptors in rodent and other mammalian tissues. However, a readily accessible, normal human tissue for studies of these drugs has not been available. We showed that freshly isolated human leukocytes can be used for this purpose. Following isolation by dextran sedimentation, leukocytes were incubated for 30 sec. in histamine concentrations from 1.0 nM to 1.0 mM. A dose-related increase in intracellular cyclic AMP was observed. When the cells were preincubated for 10 min. in the tricyclic, nortriptyline, and then challenged with histamine, the concentration of histamine needed for a comparable cyclic AMP increase was elevated 100-fold over non-preincubated cells. These results indicate that the tricyclic drug interacts with histamine receptors on leukocytes, and that these cells may be used for biochemical and clinical studies of these drugs.
journal_name
Life Scijournal_title
Life sciencesauthors
Sadava D,Wilmington Kdoi
10.1016/0024-3205(84)90441-7subject
Has Abstractpub_date
1984-12-17 00:00:00pages
2545-8issue
25eissn
0024-3205issn
1879-0631pii
0024-3205(84)90441-7journal_volume
35pub_type
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