Abstract：
AIMS:Epigenetic and genetic alterations are crucial events in the onset and progression of human cancers including colorectal cancer (CRC). This work aims to probe the relevance of lysine demethylase 5B (KDM5B) to the progression of CRC and the possible molecules involved. MATERIALS AND METHODS:KDM5B expression in CRC tissues and cells was determined. The association between KDM5B and the prognosis of patients was analyzed. Gain- and loss-of function studies of KDM5B were performed in HT-29 and KDM5B cells to explore the impact of KDM5B on cell behaviors. Expression of CC chemokine ligand 14 (CCL14) in CRC tissues and cells and its correlation with KDM5B were analyzed. Altered expression of CCL14 was introduced in CRC cells, and a Wnt/β-catenin-specific antagonist KYA1797K was induced in cells as well. KEY FINDINGS:KDM5B was abundantly expressed while CCL14 was poorly expressed in CRC tissues and cells. High KDM5B expression was relevant to poor prognosis of patients. Downregulation of KDM5B suppressed proliferation and aggressiveness of HT-29 cells, and reduced the growth of xenograft tumors in mice, while upregulation of KDM5B in SW480 cells led to reverse results. KDM5B reduced CCL14 expression through demethylation modification of H3K4me3. Upregulation of CCL14 suppressed colony formation and invasiveness of CRC cells. KDM5B downregulated CCL14 to activate the Wnt/β-catenin. Inhibition of β-catenin by KYA1797K blocked the oncogenic roles of KDM5B in cells and in xenograft tumors. SIGNIFICANCE:This study suggested that KDM5B suppresses CCL14 through demethylation modification of H3K4me3, leading to activation of the Wnt/β-catenin and the CRC progression.
journal_name
Life Scijournal_title
Life sciencesauthors
Yan G,Li S,Yue M,Li C,Kang Zdoi
10.1016/j.lfs.2020.118726subject
Has Abstractpub_date
2021-01-01 00:00:00pages
118726eissn
0024-3205issn
1879-0631pii
S0024-3205(20)31479-Xjournal_volume
264pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Oxaliplatin (l-OHP) is a key drug in therapeutic regimens for metastatic or advanced-stage colorectal cancer, but causes peripheral neuropathy as a dose-limiting adverse effect. It is reported that this peripheral neuropathy results from l-OHP accumulation in dorsal root ganglion (DRG) neurons, and that one of the...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.07.006
更新日期：2018-08-15 00:00:00
abstract：:Viperid, elapid and crotalid snake venoms were screened in vitro for antiviral activity against Sendai virus. The hemolysis of 10(8) human erythrocytes in 1 ml, caused by 70 HAU of Sendai virus, was abolished when the virions were pretreated with 10 ug of the viperid venom of Echis coloratus, and was considerably dimi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90015-h
更新日期：1992-01-01 00:00:00
abstract：:The anti-secretory activity of the competitive H2-antagonist HUK 978 was determined in rat, guinea-pig and dog. In all systems examined, HUK 978 was more potent than cimetidine and ranitidine both intravenously and orally. In addition, the compound at approximately equipotent doses as these established H2-antagonists ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90300-5
更新日期：1985-11-04 00:00:00
abstract：:alpha-Difluoromethylornithine (DFMO) directly infused into a brain-lateral ventricle (12.5, 25 and 50 micrograms/rat) dose- and time-dependently inhibited brain ODC activity. While having no influence per se on pain threshold, DFMO significantly inhibited the analgesic activity of morphine (15 mg/kg i.p.), this effect...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90614-0
更新日期：1989-01-01 00:00:00
abstract：:Bromopride (BRO) and domperidone (DOMP) are dopamine D2 blocking agents used in gastroenterology clinics because of their anti emetic effect as well as their central and peripheral actions of increasing gastrointestinal motor activity. The rationale for these experiments was to compare BRO- and DOMP-effects on plasma,...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00429-v
更新日期：1995-01-01 00:00:00
abstract：:The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.02.023
更新日期：2006-07-10 00:00:00
abstract：AIMS:C-reactive protein (CRP) is an inflammatory cytokine that has been shown to be an independent predictor of future atherothrombotic events. Hyperactivity of endothelin-1 (ET-1), a potent vasoconstrictor peptide produced by the endothelium, is linked with cardiovascular disease development and progression. ET-1 is s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.12.030
更新日期：2016-08-15 00:00:00
abstract：:We investigated the involvement of alpha 1-adrenoceptor subtypes in the positive chronotropic response to norepinephrine (NE) in neonatal rat cardiac myocytes at day 3 of culture. The cardiac myocytes at day 3 of culture exhibited a dose-dependent positive chronotropic response to NE in the presence of propranolol, a ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90155-4
更新日期：1994-01-01 00:00:00
abstract：:We investigated the effects of ketanserin, a S 2 (5-hydroxytryptamine 2; 5-HT 2)-serotonergic receptor antagonist, on cerulein-induced pancreatitis in the rat. Large pharmacological doses of cerulein induced acute pancreatitis in the rat. Ketanserin reduced the cerulein-induced increase in serum amylase concentration ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90476-6
更新日期：1992-01-01 00:00:00
abstract：:Almost all diabetic complications are known to be associated with vascular dysfunctions of different tissues. Oxidative stress, on the other hand, has been implicated in the pathogenesis of diabetes mellitus. Therefore in the present study we have investigated the correlation between redox status and oxidative stress ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00662-3
更新日期：2003-10-03 00:00:00
abstract：:Behavioral Hypersensitivity (BH) to dopamine agonists occurs following chronic treatment with most neuroleptics including haloperidol. In the present study we observed that the concurrent administration of thioridazine and haloperidol prevented the development of BH. In contrast, another neuroleptic, fluphenazine, coa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90120-7
更新日期：1986-05-12 00:00:00
abstract：:It is currently believed that oxidative stress and inflammation play a significant role in atherogenesis. Artichoke extract exhibits hypolipemic properties and contains numerous active substances with antioxidant properties in vitro. We have studied the influence of aqueous and ethanolic extracts from artichoke on int...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02136-7
更新日期：2002-11-01 00:00:00
abstract：AIMS:We recently reported that acute exposure to nicotine vasodilates the renal vasculature of male rats via facilitation of endothelial nitric oxide synthase (eNOS). In this study, we investigated whether this effect of nicotine is sexually dimorphic and the role of estrogen in modulating the nicotine effect. MAIN ME...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.11.009
更新日期：2011-01-17 00:00:00
abstract：:We have developed and utilized a photoaffinity probe to identify the adenosine transporter in cardiac sarcolemmal (SL) vesicles. The probe is an azidosalicylate derivative of adenosine made by reacting adenosine with N-hydroxysuccinimidyl-4-azidosalicylic acid (NHS-ASA). Following synthesis and radiolabeling of the pr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00268-b
更新日期：1995-01-01 00:00:00
abstract：:The present study shows that morphine reduces the pulmonary inflammatory response to intranasal influenza virus infection in rats. Rats were infected with rat-adapted influenza virus (RAIV), which is a unique infectious agent because normal rats develop an acute pulmonary inflammatory response to RAIV and rapidly clea...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00348-3
更新日期：1999-01-01 00:00:00
abstract：:The aim of the present study was to determine the role of adrenals in gonadal activity in the male toad during the breeding season. Exogenous administration of corticosterone or metapyrone for 6 days inhibited adrenal delta5-3beta(delta 5-3 beta) hydroxysteroid (delta5-3beta-HSD) and testicular 17beta (17 beta) hydrox...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00433-1
更新日期：2000-02-18 00:00:00
abstract：:Synthetic human pancreatic Growth Hormone-Releasing Factor (hpGRF) elevated the plasma concentration of growth hormone (GH) in young and adult domestic fowl. This in vivo effect of hpGRF appeared to be largely similar for both the 32 amino-acid (hpGRF 1-32) or 40 amino-acid (hpGRF 1-40) polypeptide, although the effec...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90083-3
更新日期：1984-03-19 00:00:00
abstract：:An in vivo antisense strategy was used to examine the involvement of G-protein subunits in supraspinal (intracerebroventricular; i.c.v.) alpha2-adrenoceptor-mediated antinociception. Mice that were injected with 33-mer antisense oligodeoxyribonucleotides (6 nmol) or vehicle were tested (tail-flick) with an agonist (cl...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02301-1
更新日期：1996-01-01 00:00:00
abstract：:In critically ill patients various conditions may lead to the activation of poly(ADP-ribose) polymerase (PARP). By promoting cellular energetic dysfunction, and by enhancing pro-inflammatory gene expression, PARP activation significantly contributes to the pathogenesis of shock. PARP activation is usually triggered by...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.11.001
更新日期：2008-01-16 00:00:00
abstract：:Pretreatment with a low dose of 3-nitropropionic acid (3-NPA) has been shown to induce ischemic tolerance in the gerbil hippocampus. It is well known that sublethal (2-min) ischemia also induces ischemic tolerance. To investigate the acquisition of ischemic tolerance with 3-NPA, we examined the protein expression afte...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.01.037
更新日期：2005-10-21 00:00:00
abstract：:Peptide nucleic acids (PNAs) are uncharged DNA analogs that hybridize to complementary sequences with high affinity and stability. We previously showed that PNAs, after intraperitoneal injection into rats, are effective antisense compounds in vivo. The present study was designed to test whether PNAs also have antigene...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01647-8
更新日期：2002-06-07 00:00:00
abstract：:This study examined whether there is increased production of oxygen free radicals during chronic renal failure. Rats subjected to 3/4 nephrectomy and sham operated controls were killed after 3 weeks. Lipid extracts of plasma and renal tissue were examined by HPLC and kidney specimens were also analyzed by EPR spectros...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90548-4
更新日期：1992-01-01 00:00:00
abstract：:We used a sensitive and specific radioimmunoassay for acetylcholine (ACh), and detected significant amounts of ACh in the blood of various mammals, including humans. About 60% of human blood ACh was localized in mononuclear leukocytes. Human leukemic T-cell lines, used as T-lymphocyte models, contained both ACh and ch...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(98)00131-3
更新日期：1998-01-01 00:00:00
abstract：:Protein phosphorylation is a key posttranslational modification mechanism controlling the conformation and activity of many proteins. Increasing evidence has implicated an essential role of phosphorylation by several major protein kinases in promoting and maintaining opioid tolerance. We review some of the most recent...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2006.05.023
更新日期：2006-09-27 00:00:00
abstract：AIMS:The rational basis that explains the benefits of exercise therapy on Chagas cardiomyopathy (ChC) is poorly understood. This study investigated the impact of an exercise program on exercise performance, heart parasitism, immunoinflammatory response, fibrogenesis, oxidative damage, and cardiomyocytes contractility i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.03.053
更新日期：2016-05-01 00:00:00
abstract：:Promoter methylation is an important mechanism of regulating E-cadherin expression. Methylation-specific PCR (MSP) assay was done to evaluate the promoter methylation status of E-cadherin gene in primary tumor samples from 23 cases of Chinese women with invasive ductal breast cancers. Western blotting assay was employ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01843-x
更新日期：2002-08-09 00:00:00
abstract：AIMS:Recent evidence has shown that systemic administration of sulfonylureas and biguanides block the diclofenac-induced antinociception, but not the effect produced by indomethacin. However, there are no reports about the peripheral interaction between analgesics and the biguanides metformin and phenformin. Therefore,...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.10.009
更新日期：2012-01-02 00:00:00
abstract：AIMS:Few studies have compared the interaction of single and repeated administration of amitriptyline (amit) with the nitrergic system and glutamatergic system in the experimental model of neuropathic pain. We aimed to evaluate the antinociceptive effect of single and repeated administration of amit and to assess wheth...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116752
更新日期：2019-09-15 00:00:00
abstract：:Although intraepithelial T lymphocytes of the large intestine (LI) are known to differ from those of the small intestine (SI) in phenotype and function, differences in LI and SI lamina propria (LP) lymphocyte populations have not been clearly established. In this work we found striking phenotypic differences between S...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.08.042
更新日期：2005-04-29 00:00:00
abstract：:Pulmonary fibrosis (PF), which is characterized by excessive matrix formation, may ultimately lead to irreversible lung damage and thus death. Fibroblast activation has been regarded as a central event during PF pathogenesis. In our previous study, we confirmed that the miR-627/high-mobility group box protein 1 (HMGB1...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2021.119085
更新日期：2021-01-20 00:00:00
