Nicotine administration to rats: methodological considerations.

Abstract:

:The effects of nicotine on normal physiological function are of increasing concern. Preliminary to studies on the effects of prenatal exposure to nicotine, we examined methods of administering nicotine to rats. Drinking water containing nicotine was not palatable to rats and was an unsatisfactory method in our hands, producing weight loss and large decreases in fluid intake. Administration of nicotine in a complete liquid diet produced better results but the data suggest that oral administration of nicotine may interfere with absorption of some nutrients. Osmotic mini-pumps were found to be the best mechanism of nicotine delivery of those tried. There were no significant effects on food or water intake nor on weight gain, particularly when using a short term anesthetic for pump implantation. Plasma nicotine and cotinine levels were directly correlated to dose of nicotine delivered. Plasma nicotine levels similar to levels reported in humans were obtained.

journal_name

Life Sci

journal_title

Life sciences

authors

Murrin LC,Ferrer JR,Zeng WY,Haley NJ

doi

10.1016/0024-3205(87)90020-8

subject

Has Abstract

pub_date

1987-04-27 00:00:00

pages

1699-708

issue

17

eissn

0024-3205

issn

1879-0631

pii

0024-3205(87)90020-8

journal_volume

40

pub_type

杂志文章
  • T-type calcium channel antagonist, TTA-A2 exhibits anti-cancer properties in 3D spheroids of A549, a lung adenocarcinoma cell line.

    abstract:AIMS:Despite the advanced cancer treatments, there is increased resistance to chemotherapy and subsequent mortality. In lack of reliable data in monolayer cultures and animal models, researchers are shifting to 3D cancer spheroids, which represents the in vivo robust tumour morphology. Calcium is essential in cell sign...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2020.118291

    authors: Kumari N,Bhargava A,Rath SN

    更新日期:2020-11-01 00:00:00

  • 3H-5-hydroxytryptamine accumulation by rat brain synaptic vesicles in a membrane-impermeant medium, and selective reduction by 5,7-dihydroxytryptamine.

    abstract::In order to examine possible selectivity of amine uptake by synaptic vesicles, the ATP-stimulated accumulation of 3H-5-hydroxytryptamine (5HT) by synaptic vesicles from rat whole brain was examined in a medium comprised largely of membrane-impermeant anions (d-tartrate). Such media have previously been shown to stabil...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(86)90174-8

    authors: Ruth JA,Cuizon JV,Park SH,Ullman EA,Wilson WR

    更新日期:1986-03-31 00:00:00

  • Proconflict effect of carbon dioxide inhalation in rats.

    abstract::The effect of brief inhalation of carbon dioxide (CO2) was studied in a conflict situation (Vogel test) in the rat. This treatment, which inhibits gamma-aminobutyric acid (GABA)-mediated transmission in rat brain and induces anxiety and panic attacks in humans, elicited a proconflict effect. Exposure of rats for 1 min...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(95)00093-3

    authors: Cuccheddu T,Floris S,Serra M,Porceddu ML,Sanna E,Biggio G

    更新日期:1995-03-10 00:00:00

  • U50,488H postconditioning reduces apoptosis after myocardial ischemia and reperfusion.

    abstract:AIMS:Evidence has indicated U50,488H, a selective κ-opioid receptor (κ-OR) agonist, administered before ischemia attenuates apoptosis and infarction during ischemia and reperfusion (I/R). However, it remains unclear whether U50,488H postconditioning reduces apoptosis during I/R. This study was designed, therefore, to t...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2010.10.018

    authors: Tong G,Sun Z,Wei X,Gu C,Kaye AD,Wang Y,Li J,Zhang Q,Guo H,Yu S,Yi D,Pei J

    更新日期:2011-01-03 00:00:00

  • Changes in dopamine metabolism in rat forebrain regions after cessation of long-term fluoxetine treatment: relationship with brain concentrations of fluoxetine and norfluoxetine.

    abstract::We examined the effects of repeated administration of the selective serotonin uptake inhibitor (SSRI) fluoxetine (Flx) (5, 10, or 15 mg/kg i.p., twice daily for 21 days) on brain and plasma concentrations of the parent drug and its active desmethyl metabolite, norfluoxetine (NFlx), in rats during the 21-day regimen as...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(94)00821-3

    authors: Gardier AM,Lepoul E,Trouvin JH,Chanut E,Dessalles MC,Jacquot C

    更新日期:1994-01-01 00:00:00

  • Selective effects of diltiazem, a benzothiazepine calcium channel blocker, and diazepam, and other benzodiazepines on the Na+/Ca2+ exchange carrier system of heart and brain mitochondria.

    abstract::Diltiazem, diazepam, and clonazepam effectively inhibit Na+-induced Ca2+ release from mitochondria isolated from rabbit heart and rat brain. At 10 mM NaCl, the 50% inhibition of the rate of Ca2+ release occurred at 5, 7, 40 and 350 microM of clonazepam d-cis-diltiazem, diazepam and 1-cis-diltiazem, respectively. Ro 15...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(83)90319-3

    authors: Matlib MA,Schwartz A

    更新日期:1983-06-20 00:00:00

  • Serotonin-mediated acute insulin resistance in the perfused rat hindlimb but not in incubated muscle: a role for the vascular system.

    abstract::We have recently shown that the vasoconstrictor serotonin (5-HT) inhibits oxygen uptake in perfused hindlimb possibly due to vascular shunting. Thus in the present study the effect of 5-HT on insulin-mediated glucose uptake was assessed. Rat hindlimbs were perfused at constant flow with medium containing 8.3 mM glucos...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90563-i

    authors: Rattigan S,Dora KA,Colquhoun EQ,Clark MG

    更新日期:1993-01-01 00:00:00

  • Biosynthetic arginine decarboxylase in phytopathogenic fungi.

    abstract::It has been reported that while bacteria and higher plants possess two different pathways for the biosynthesis of putrescine, via ornithine decarboxylase (ODC) and arginine decarboxylase (ADC); the fungi, like animals, only use the former pathway. We found that contrary to the earlier reports, two of the phytopathogen...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(89)90317-2

    authors: Khan AJ,Minocha SC

    更新日期:1989-01-01 00:00:00

  • Influence of sympathectomy on alpha 2 adrenoceptor binding sites in canine blood vessels.

    abstract::The effects of denervation of alpha 2 adrenoceptor binding sites were examined in canine arteries and veins. Denervation of the lower abdominal aorta, renal and femoral arteries and femoral veins marked reduced vessel norepinephrine concentrations. Denervation had little effect on the concentration of alpha 2 adrenoce...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(83)80005-8

    authors: Bobik A,Anderson WP

    更新日期:1983-07-25 00:00:00

  • Effects of different physical training protocols on ventricular oxidative stress parameters in infarction-induced rats.

    abstract:AIM:Physical exercise is important in the prevention and treatment of cardiovascular diseases. Nevertheless, controversy remains around type and intensity of effort required for significant biochemical protective changes. This study investigates two exercise protocols on ventricular oxidative parameters in rats post-in...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2012.01.018

    authors: Pinho CA,Tromm CB,Tavares AM,Silva LA,Silveira PC,Souza CT,Benetti M,Pinho RA

    更新日期:2012-04-09 00:00:00

  • Mode of action of antiprotozoan agents. Electron transfer and oxy radicals.

    abstract::Cyclic voltammetry data were obtained for most of the main classes of antiprotozoan agents, specifically, nitroheterocycles, quinones, metal complexes and derivatives, iminium-type ions, and azo compounds. The reductions were generally reversible in the range of -0.3 to -0.9 V. Catalytic production of oxidative pressu...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(87)90740-5

    authors: Ames JR,Ryan MD,Kovacic P

    更新日期:1987-10-19 00:00:00

  • Endurance exercise causes mitochondrial and oxidative stress in rat liver: effects of a combination of mitochondrial targeting nutrients.

    abstract:AIMS:Endurance exercise causes fatigue due to mitochondrial dysfunction and oxidative stress. In order to find an effective strategy to prevent fatigue or enhance recovery, the effects of a combination of mitochondrial targeting nutrients on physical activity, mitochondrial function and oxidative stress in exercised ra...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2009.11.003

    authors: Sun L,Shen W,Liu Z,Guan S,Liu J,Ding S

    更新日期:2010-01-02 00:00:00

  • Effects of the soluble fiber complex PolyGlycopleX® (PGX®) on glycemic control, insulin secretion, and GLP-1 levels in Zucker diabetic rats.

    abstract:AIMS:The effects of the novel water soluble, viscous fiber complex PolyGlycopleX® [(α-D-glucurono-α-D-manno-β-D-manno-β-D-gluco), (α-L-gulurono-β-D mannurono), β-D-gluco-β-D-mannan (PGX®)] on body weight, food consumption, glucose, insulin, and glucagon-like peptide (GLP-1) levels were determined in Zucker diabetic rat...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2010.11.014

    authors: Grover GJ,Koetzner L,Wicks J,Gahler RJ,Lyon MR,Reimer RA,Wood S

    更新日期:2011-02-28 00:00:00

  • Time dependent protection of amifostine from renal and hematopoietic cisplatin induced toxicity.

    abstract::Efficacy of chemotherapy may be maximized and its toxicity can be minimized if drugs would be administered at specified daily times. The present study was aimed to examine if the protection of amifostine against cisplatin toxicity is time dependent. Amifostine is an organic thiophosphate that protects selectively norm...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2004.09.028

    authors: Asna N,Lewy H,Ashkenazi IE,Deutsch V,Peretz H,Inbar M,Ron IG

    更新日期:2005-03-04 00:00:00

  • Comparison between electronic cigarette refill liquid and nicotine on metabolic parameters in rats.

    abstract:AIMS:Nicotine is known to promote body weight loss and to disturb glucose homeostasis and lipoprotein metabolism. Electronic cigarettes, as a substitute to nicotine, are becoming increasingly popular, although there is no evidence regarding their safety. Considering the dearth of information about e-cigarette toxicity,...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2015.12.049

    authors: El Golli N,Dkhili H,Dallagi Y,Rahali D,Lasram M,Bini-Dhouib I,Lebret M,Rosa JP,El Fazaa S,Allal-El Asmi M

    更新日期:2016-02-01 00:00:00

  • Relationship between urinary excretion of homovanillic acid and norepinephrine metabolites in normal subjects and patients with orthostatic hypotension.

    abstract::Patients with neurogenic orthostatic hypotension due to multiple system atrophy (MSA) or pure autonomic failure (PAF) excrete lower amounts of homovanillic acid (HVA) than do normal subjects. There is a highly significant correlation between the rates of excretion of HVA and norepinephrine metabolites. The regression ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(88)90289-5

    authors: Kopin IJ,Oliver JA,Polinsky RJ

    更新日期:1988-01-01 00:00:00

  • Functional studies with the opioid peptide dynorphin: acute effects of injections into the substantia nigra reticulata of naive rats.

    abstract::When injected into the substantia nigra reticulata of naive rats, dynorphin-(1-17) caused a dose dependent contralateral rotation which was inhibited by naloxone in a dose dependent manner. Met-enkephalin injected in the same coordinates as the ones used with dynorphin also induced rotation but of lower intensity, whi...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(83)90563-5

    authors: Herrera-Marschitz M,Hökfelt T,Ungerstedt U,Terenius L

    更新日期:1983-01-01 00:00:00

  • Modulation of benzodiazepine by lysine and pipecolic acid on pentylenetetrazol-induced seizures.

    abstract::L-lysine, an essential amino acid for man and animals, and its metabolite pipecolic acid (PA) have been studied for their effects on pentylenetetrazol (PTZ)-induced seizures in mice. L-Lysine or L-PA i.p. significantly increased clonic and tonic latencies in a dose-dependent manner against 90 mg/kg PTZ-induced seizure...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(88)90207-x

    authors: Chang YF,Hargest V,Chen JS

    更新日期:1988-01-01 00:00:00

  • Evidence that diclofenac and celecoxib are thyroid hormone receptor beta antagonists.

    abstract:UNLABELLED:Long term use of NSAIDs is linked to side effects such as gastric bleeding and myocardial infarction. AIMS:Use of in silico methods and pharmacology to investigate the potential for NSAIDs diclofenac, celecoxib and naproxen to bind to nuclear receptors. MATERIALS AND METHODS:In silico screening predicted t...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2016.01.013

    authors: Zloh M,Perez-Diaz N,Tang L,Patel P,Mackenzie LS

    更新日期:2016-02-01 00:00:00

  • The role of fatty acid amide hydrolase inhibition in nicotine reward and dependence.

    abstract::The endogenous cannabinoid anandamide (AEA) exerts the majority of its effects at CB1 and CB2 receptors and is degraded by fatty acid amide hydrolase (FAAH). FAAH KO mice and animals treated with FAAH inhibitors are impaired in their ability to hydrolyze AEA and other non-cannabinoid lipid signaling molecules, such as...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2012.05.015

    authors: Muldoon PP,Lichtman AH,Parsons LH,Damaj MI

    更新日期:2013-03-19 00:00:00

  • Photodynamic effect of novel chlorin e6 derivatives on a single nerve cell.

    abstract::Chlorin e(6) and its derivatives are promising sensitizers for photodynamic therapy (PDT). In order to compare the photodynamic effects of 8 novel derivatives of chlorin e(6) and to explore some mechanisms of their effects at the cellular level, we studied PDT-induced changes in bioelectric activity of crayfish mechan...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2003.09.053

    authors: Uzdensky AB,Dergacheva OY,Zhavoronkova AA,Reshetnikov AV,Ponomarev GV

    更新日期:2004-03-12 00:00:00

  • Additive effects of combined valsartan and spironolactone on cardiac aldosterone escape in spontaneously hypertensive rats.

    abstract::The additive effects of combined valsartan and spironolactone on plasma and cardiac aldosterone escape were evaluated in spontaneously hypertensive rats (SHRs). Twenty-four SHRs were treated with valsartan (30 mg/kg body weight per day), spironolactone (20 mg/kg body weight per day) and a combination of both for 4 mon...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2004.04.012

    authors: Liang YH,Wang JM,Zhou Y,Jiang XJ,Jiang H,Huang CX

    更新日期:2004-08-27 00:00:00

  • Firing properties of substantia nigra dopaminergic neurons in freely moving rats.

    abstract::Single unit recordings were obtained from putative dopaminergic neurons in the substantia nigra of awake, freely moving rats. The cells exhibited waveforms, range of firing rates and types of firing patterns identical to those of identified DA neurons of anesthetized or paralyzed rats. Two firing patterns were observe...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(85)90448-5

    authors: Freeman AS,Meltzer LT,Bunney BS

    更新日期:1985-05-20 00:00:00

  • Protective properties of artichoke (Cynara scolymus) against oxidative stress induced in cultured endothelial cells and monocytes.

    abstract::It is currently believed that oxidative stress and inflammation play a significant role in atherogenesis. Artichoke extract exhibits hypolipemic properties and contains numerous active substances with antioxidant properties in vitro. We have studied the influence of aqueous and ethanolic extracts from artichoke on int...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(02)02136-7

    authors: Zapolska-Downar D,Zapolski-Downar A,Naruszewicz M,Siennicka A,Krasnodebska B,Kołdziej B

    更新日期:2002-11-01 00:00:00

  • Effects of (-)-epigallocatechin-3-gallate in Ca2+ -permeable non-selective cation channels and voltage-operated Ca2+ channels in vascular smooth muscle cells.

    abstract::The effects of (-)-epigallocatechin-3-gallate (EGCG), the most abundant catechin of tea, on Ca(2+)-permeable non-selective cation currents (NSCC) and voltage-operated Ca(2+) channels (VOCC) have been investigated in cultured rat aortic smooth muscle cells using the whole-cell voltage-clamp technique. Under the Cs(+)/t...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2007.04.005

    authors: Campos-Toimil M,Orallo F

    更新日期:2007-05-16 00:00:00

  • The presence of statocysts and statoliths in social wasps (Hymenoptera, Vespinae).

    abstract::Static sense organs composed of a hollow statocyst surrounded by sensory hairs and containing aggregate bodies (statoliths) have been detected in various species of Vespinae. On the frons in the groove traversing its center (the frontal groove) there is a deep pit and around it a membrane enclosing a cyst-like sac. In...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(83)90833-0

    authors: Ishay JS,Shimony T,Arcan L

    更新日期:1983-04-11 00:00:00

  • Tet3 enhances IL-6 expression through up-regulation of 5-hmC in IL-6 promoter in chronic hypoxia induced atherosclerosis in offspring rats.

    abstract:AIMS:Tet1, Tet2, and interleukin-6 (IL-6) have been linked to atherosclerosis. Whether Tet3 has a relationship with atherosclerosis and IL-6 was unknown. This study aims to determine the link between Tet3 and IL-6, and the role of Tet3 in prenatal hypoxia-induced atherosclerosis in offspring rats. MAIN METHODS:Pregnan...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2019.116601

    authors: Zhang P,Chen X,Zhang Y,Su H,Zhang Y,Zhou X,Sun M,Li L,Xu Z

    更新日期:2019-09-01 00:00:00

  • Studies on in vitro effect of free fatty acids on water content and osmotic fragility of rabbit (Lepus cuniculus) erythrocytes.

    abstract::The effects of free fatty acids in the blood on osmotic fragility and water content of erythrocytes when combined with hyperthermia were investigated. The isotonic buffer, containing the fatty acid, was added to the erythrocyte suspension to a final concentration of 200 microM/L. The samples were kept for one hour in ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(97)01184-3

    authors: Kogawa H,Yabushita N,Nakajima T,Kageyama K

    更新日期:1998-01-01 00:00:00

  • Protective effect of a molecular chaperone inducer, paeoniflorin, on the HCl- and ethanol-triggered gastric mucosal injury.

    abstract:AIMS:We previously found that paeoniflorin, a major constituent of Paeonia lactiflora Pall, could induce heat shock proteins (HSPs) in cultured mammalian cells without apparent toxicity (Yan et al. 2004). We here investigated the induction of HSPs by paeoniflorin in mouse stomach and the effect of paeoniflorin on the H...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2010.12.014

    authors: Asai M,Kawashima D,Katagiri K,Takeuchi R,Tohnai G,Ohtsuka K

    更新日期:2011-02-14 00:00:00

  • Markedly reduced blood pressure responsiveness in endotoxemic rats; reversal by neuropeptide Y.

    abstract::This study in conscious normotensive rats was performed to assess the effect of the vasoconstrictor peptide, neuropeptide Y (NPY), on blood pressure responsiveness to exogenous norepinephrine in endotoxaemia. NPY and endotoxin were infused at doses which had no effect on blood pressure, whether given alone or in combi...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(87)90442-5

    authors: Evéquoz D,Waeber B,Corder R,Nussberger J,Gaillard R,Brunner HR

    更新日期:1987-12-07 00:00:00