Abstract：
:Adenylate cyclase activity in isolated rat liver plasma membranes was inhibited by NADH in a concentration-dependent manner. Half-maximal inhibition of adenylate cyclase was observed at 120 microM concentration of NADH. The effect of NADH was specific since adenylate cyclase activity was not altered by NAD+, NADP+, NADPH, and nicotinic acid. The ability of NADH to inhibit adenylate cyclase was not altered when the enzyme was stimulated by activating the cyclase was not altered when the enzyme was stimulated by activating the Gs regulatory element with either glucagon or cholera toxin. Similarly, inhibition of Gi function by pertussis toxin treatment of membranes did not attenuate the ability of NADH to inhibit adenylate cyclase activity. Inhibition of adenylate cyclase activity to the same extent in the presence and absence of the Gpp (NH) p suggested that NADH directly affects the catalytic subunit. This notion was confirmed by the finding that NADH also inhibited solubilized adenylate cyclase in the absence of Gpp (NH)p. Kinetic analysis of the NADH-mediated inhibition suggested that NADH competes with ATP to inhibit adenylate cyclase; in the presence of NADH (1 mM) the Km for ATP was increased from 0.24 +/- 0.02 mM to 0.44 +/- 0.08 mM with no change in Vmax. This observation and the inability of high NADH concentrations to completely inhibit the enzyme suggest that NADH interacts at a site(s) on the enzyme to increase the Km for ATP by 2-fold and this inhibitory effect is overcome at high ATP concentrations.
journal_name
Life Scijournal_title
Life sciencesauthors
Nair BG,Patel TBdoi
10.1016/0024-3205(91)90177-dsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
915-23issue
12eissn
0024-3205issn
1879-0631pii
0024-3205(91)90177-Djournal_volume
49pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:The in vivo dipyridamole treatment for 16 days produced a significant decrease in chick plasma cholesterol, mainly due to the esterified form. This effect was especially patent in the VLDL + LDL fraction. Similar results were observed in triglyceride content. To our knowledge, this is the first report on this hypolipi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90574-u
更新日期：1991-01-01 00:00:00
abstract：:Vitamin E treatment was found to lower blood pressure, and increase membrane fluidity in rats. The objectives of this study were to investigate the effects of the antioxidant, vitamin E, on the blood pressure and erythrocyte membrane fluidity in spontaneously hypertensive (SHR) and normotensive (WKY) rats. Membrane fl...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00775-x
更新日期：2000-09-01 00:00:00
abstract：:Involvement of cyclic AMP-dependent protein kinase in the desensitization of GABAB receptor expressed in Xenopus oocytes was studied. GABA produced an outward current in the presence of bicuculline, under voltage-clamp conditions. Baclofen mimicked the effect of GABA, thereby indicating that the response to GABA in th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02235-1
更新日期：1995-11-17 00:00:00
abstract：AIMS:Stroke-prone spontaneously hypertensive rats (SHRSP) show significantly lower body weight than normotensive Wistar-Kyoto rats (WKY). Our hypotheses are as follows: weight loss of the skeletal muscle is related to hypertension-related diseases, and muscle hypotrophy is useful as a therapeutic target for hypertensio...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116919
更新日期：2019-11-15 00:00:00
abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficient red blood cells (RBCs) are known to be more susceptible to oxidant-induced hemolysis. Erythrocytes from G6PD-deficient individuals are significantly more susceptible to Ca(2+)-induced vesiculation than normal control cells. The enhanced susceptibility of G6PD-deficien...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00377-3
更新日期：1996-01-01 00:00:00
abstract：AIMS:The long non-coding RNA (lncRNA) was reported to be involved in the progress of various cancers, however, its effect in colorectal cancer (CRC) remains unknown. The goal of the present study is to investigate the function role of lncRNA PCAT-1 in colorectal cancer. MAIN METHODS:The expression of lncRNA PCAT-1 in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.08.024
更新日期：2017-11-01 00:00:00
abstract：:Two recently isolated peptides, endomorphin-1 (Tyr-Pro-Trp-Phe-NH2) and endomorphin-2 (Tyr-Pro-Phe-Phe-NH2), are highly selective micro-opioid receptor agonists with analgesic actions in the tail-flick test. To further assess the analgesic properties of these peptides, the effects of endomorphin-1, endomorphin-2, and ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00689-5
更新日期：2000-07-14 00:00:00
abstract：AIMS:With the improvement of the survival rates in children acute lymphoblastic leukemia (ALL), some children ALL survivors show impaired cognitive function. Methotrexate (MTX), an essential component in ALL treatment, has been reported to be related to neurologic sequelae and to increased oxidative stress through its ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116931
更新日期：2020-02-01 00:00:00
abstract：:Although (-)-125I-iodopindolol (IPIN) can be used to label beta-adrenergic receptors in the central nervous system (CNS) in vivo, use of this ligand for receptor imaging studies in humans may be limited due to its relatively poor penetration into the CNS. A series of derivatives related to pindolol was therefore studi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90688-6
更新日期：1987-08-24 00:00:00
abstract：:We examined the effects of cyrneine A, a novel diterpene isolated from the mushroom Sarcodon cyrneus, on morphology of rat pheochromocytoma cells (PC12). In response to cyrneine A, PC12 cells extended their neurites, an effect partially blocked by the extracellular signal-regulated kinase (ERK) kinase inhibitor PD9805...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.01.057
更新日期：2007-04-10 00:00:00
abstract：:The present study was carried out in order to identify the changes in expression of multidrug resistance-associated protein (Mrp) 2 and P-glycoprotein (P-gp) in the intestine and remote organs after intestinal ischemia-reperfusion (I/R). Mrp2 expression in the jejunum and liver was decreased at 6 h after I/R. This dec...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.04.019
更新日期：2008-06-20 00:00:00
abstract：:The effect of cadmium (Cd2+) was studied in vitro on the flavokinase (ATP : riboflavin 5'-phosphotransferase, EC 2.7.1.26) activity purified from rat liver. Cadmium inhibited flavokinase activity in a concentration-dependent manner and the effect was completely reversed by increasing concentration of zinc (Zn2+), indi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00151-3
更新日期：1997-01-01 00:00:00
abstract：AIMS:We investigated the effects of chemical stimulation of cerebellum fastigial nucleus (FN) on the chronic visceral hypersensitivity (CVH) and its possible mechanism in rats. MAIN METHODS:We stimulated the FN by microinjecting glutamate into the FN, in order to explore whether the cerebellum fastigial nucleus played...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.04.043
更新日期：2018-06-15 00:00:00
abstract：:Leukemias are a heterogenous group of diseases characterized by uncontrolled proliferation of abnormal blood cells of hematopoietic system. Evodiamine, a characteristic alkaloid extracted from Evodia fruits, has been reported to exhibit inhibitory effect on cell proliferation and migration in several types of cancer c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.11.025
更新日期：2004-05-21 00:00:00
abstract：:Histogranin is a naturally-occurring pentadecapeptide with a structure 80% homologous with that a fragment-(86-100) of histone H4. First isolated from bovine adrenal medulla, the peptide was also shown to be present in the pituitary, brain, adrenal glands, blood plasma, lungs and spleen. At the subcellular level, hist...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(95)00068-2
更新日期：1995-03-03 00:00:00
abstract：:Hexokinase and glucose-6-phosphate dehydrogenase activities were studied in brain regions after intraventricular injection of kainic acid. Hexokinase activity was decreased by 10-15% in various regions while glucose-6-phosphate dehydrogenase activity remained unaltered. Soluble hexokinase activity, which remained the ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90471-m
更新日期：1991-01-01 00:00:00
abstract：:In an in vitro bioassay system for adrenocorticotropic hormone using isolated rat adrenal cells, kaurenol, a diterpene alcohol, stimulated corticosterone production and augmented the steroidogenic effect of adrenocorticotropin or forskolin, dose-dependently. Kaurenol had no effect on cyclic AMP production by the cells...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90070-6
更新日期：1986-02-03 00:00:00
abstract：:Effects of intracellular accumulation of isoproterenol (ISO) on lactate production were examined in perfused rat heart. The lactate production during ISO perfusion in rat heart was increased and subsequent addition of an inhibitor of catechol-O-methyl transferase (COMT) further enhanced the production, and the enhance...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90284-x
更新日期：1990-01-01 00:00:00
abstract：:Peptide Histidine Isoleucine (PHI) is generally considered a low affinity agonist for Vasoactive Intestinal Peptide (VIP) receptors. In this study, we investigated the presence and characteristics of [125I]PHI binding sites on rat liver membranes. Detergents at nonsolubilizing concentrations (1 mM CHAPS or 0.01% Tween...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90661-8
更新日期：1987-11-23 00:00:00
abstract：AIM:Irisin, the adipomyokine, released mainly by exercised muscles, participate in the browning of adipose tissue and contribute to the restriction of insulin resistance and diabetes mellitus 2 development. Because of the limited reports describing the effect of irisin on inflammation and immunocompetent cells activati...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.03.011
更新日期：2017-05-01 00:00:00
abstract：:The effect of triiodothyronine (T3) on Na+,K(+)-ATPase activity of K562 human erythroleukemic cell was studied to understand why the erythrocyte sodium pump activity is decreased in hyperthyroidism. Na+,K(+)-ATPase activity of K562 cell lysates was assayed by measuring the release of inorganic phosphate (Pi) from ATP....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90107-z
更新日期：1992-01-01 00:00:00
abstract：:Sodium spirulan (Na-SP) is a sulfated polysaccharide with M(r) approximately 220,000 isolated from the blue-green alga Spirulina platensis. The polysaccharide consists of two types of disaccharide repeating units, O-hexuronosyl-rhamnose (aldobiuronic acid) and O-rhamnosyl-3-O-methylrhamnose (acofriose) with sulfate gr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.061
更新日期：2004-03-26 00:00:00
abstract：:Triiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116556
更新日期：2019-08-15 00:00:00
abstract：:The changes in the energy charge (ATP + 0.5 ADP)/(ATP + ADP + AMP) levels of the remnant liver were studied in relation to changes in the incorporation rate of methyl-3H-thymidine into DNA, the blood ratio of acetoacetate/beta-hydroxybutyrate and indocyanine green (ICG) clearance in 70% hepatectomized rabbits. The ene...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90765-2
更新日期：1982-08-16 00:00:00
abstract：:Alzheimer's disease (AD) involves selective loss of muscarinic m2, but not m1, subtype neuroreceptors in the posterior parietal cortex of the human brain. Emission tomographic study of the loss of m2 receptors in AD is limited by the fact that there is currently no available m2-selective radioligand which can penetrat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90161-u
更新日期：1993-01-01 00:00:00
abstract：:The effects of longer lasting blood pressure changes on the release of endogenous catecholamines (CA) in limbic and hypothalamic areas were studied in anaesthetized rats. For this purpose the central nucleus of the amygdala (AC), ventral hippocampus (VH) and medial hypothalamus (MH) were simultaneously superfused thro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90496-6
更新日期：1987-07-13 00:00:00
abstract：AIMS:Acetylcholine (ACh) has been shown to increase ion and water excretion in the kidneys, resulting in hypotension. However, no evidence of renal parasympathetic innervation has been shown, and the source of ACh acting on nephrons is still unknown. The aim of the present study was to identify ACh-producing cells in t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.07.011
更新日期：2011-09-12 00:00:00
abstract：:Anorexia, nausea/emesis and peripheral sensorial neuropathy are frequent adverse effects associated with chemotherapy. Cannabinoids have been proposed to alleviate these effects, but their preventive properties in long-term experimental models have not been tested. This study was conducted to determine whether or not ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.06.012
更新日期：2007-07-19 00:00:00
abstract：:Because of the low selectivity of available ligands, pharmacological approaches to elucidate the functional difference among muscarinic acetylcholine receptor (mAChR) subtypes have been problematic. As an alternative approach, we have established a series of mutant mouse lines deficient in each mAChR subtype (mAChR KO...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2004.05.034
更新日期：2004-11-05 00:00:00
abstract：AIMS:In myocardial ischemia-reperfusion (MI/R) injury, impaired autophagy function worsens cardiomyocyte death. AMP-activated protein kinase (AMPK) is a heterotrimeric protein that plays an important role in cardioprotection and myocardial autophagic function. AMPKα1 and α2 are localized primarily in the cytoplasm and ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.04.002
更新日期：2019-05-15 00:00:00
