Abstract：
:Involvement of cyclic AMP-dependent protein kinase in the desensitization of GABAB receptor expressed in Xenopus oocytes was studied. GABA produced an outward current in the presence of bicuculline, under voltage-clamp conditions. Baclofen mimicked the effect of GABA, thereby indicating that the response to GABA in the presence of bicuculline is mediated by stimulation of GABAB receptor. The GABAB receptor-mediated response was suppressed by dibutyric cyclic AMP and forskolin, but not by 1,9-dideoxy-forskolin, and the effect of dibutyric cyclic AMP was inhibited by H-8. Application of GABA for 1 min at 20-min intervals induced a reliable response, while that at 10-min intervals produced a desensitization. The desensitization was also partially prevented by H-8. Dibutyric cyclic GMP, 8-bromo-cyclic GMP and Na-nitroprusside did not affect the GABAB receptor-mediated response. Thus, cyclic AMP-dependent protein kinase, but not cyclic GMP-dependent protein kinase participates in desensitization of the GABAB receptor expressed in Xenopus oocytes.
journal_name
Life Scijournal_title
Life sciencesauthors
Yoshimura M,Yoshida S,Taniyama Kdoi
10.1016/0024-3205(95)02235-1subject
Has Abstractpub_date
1995-11-17 00:00:00pages
2397-401issue
26eissn
0024-3205issn
1879-0631pii
0024320595022351journal_volume
57pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:In vitro effects of medicinal plant extracts from the pericarpium of Citrus reticulata (cv Jiao Gan) (PCRJ) on the growth and differentiation of a recently characterized murine myeloid leukemic cell clone WEHI 3B (JCS) were investigated. Extracts of PCRJ not only inhibited the proliferation of JCS cells in a dose depe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00088-4
更新日期：1996-01-01 00:00:00
abstract：:This study was undertaken to investigate whether or not alpha 2-adrenergic pathways would negatively modulate the hypothalamic somatostatin release in rats. To induce pharmacological changes in SS release, three groups of male Sprague-Dawley rats (n = 30/group) were separately anesthetized by ip administration of uret...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90277-a
更新日期：1993-01-01 00:00:00
abstract：:The hypothermia induced by apomorphine, a mixed dopamine (DA) agonist in male Swiss-Webster mice, was not blocked by the selective D-1 antagonist SCH 23390 but was completely blocked by the selective D-2 antagonists haloperidol, sulpiride and YM-09151-2. The selective D-1 agonist SKF 38393 did not elicit hypothermic r...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90515-2
更新日期：1988-01-01 00:00:00
abstract：AIMS:Paraquat (PQ) is known to induce pulmonary injury via a redox cyclic reaction. The present study was aimed to determine the protective effects of quercetin against PQ-induced pulmonary injury in association with its antioxidant activity. MAIN METHODS:Male rats were challenged acutely by PQ (50 mg/kg, i.p.) with o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.06.011
更新日期：2010-07-31 00:00:00
abstract：:Clinical and preclinical studies have suggested that fluctuations in the peripheral and brain concentrations of progesterone and deoxycorticosterone and its metabolites 3alpha,5alpha-tetrahydroprogesterone and 3alpha,5alpha-tetrahydrodeoxycorticosterone, respectively, might play an important role in certain pathologic...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2003.12.002
更新日期：2004-05-14 00:00:00
abstract：:This study investigated the effects of captopril, hydralazine and losartan on the locomotor activity, tailflick and hot plate latencies in spontaneously hypertensive rats (SHR) and their genetic controls the Wistar-Kyoto rat (WKY). The increased hot plate latencies normally exhibited by the spontaneously hypertensive ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00043-6
更新日期：1995-02-17 00:00:00
abstract：AIMS:The effects of the novel water soluble, viscous fiber complex PolyGlycopleX® [(α-D-glucurono-α-D-manno-β-D-manno-β-D-gluco), (α-L-gulurono-β-D mannurono), β-D-gluco-β-D-mannan (PGX®)] on body weight, food consumption, glucose, insulin, and glucagon-like peptide (GLP-1) levels were determined in Zucker diabetic rat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.11.014
更新日期：2011-02-28 00:00:00
abstract：AIMS:Previous work has demonstrated that ketogenic diets promote white fat browning; however, the exact mechanisms underlying this phenomenom have yet to be elucidated. Recently, an in vitro study showed that supraphysiological concentrations of β-hydroxybutyrate (βHB) had a strong influence on the induction of adipocy...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116683
更新日期：2019-09-01 00:00:00
abstract：:Benzo(a)pyrene induces cytochrome P-450 1A1 (CYP1A1) expression in rat polymorphonuclear leukocytes (PMNs) that upregulates expression of inducible nitric oxide synthase (iNOS). In the present study, the involvement of secondary signaling molecules in CYP1A1-mediated augmentation of iNOS expression in benzo(a)pyrene-t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.09.032
更新日期：2007-11-30 00:00:00
abstract：:The effects of the novel anticonvulsant felbamate, which binds to the 5-7 dichlorokynurenic binding sites, were tested towards the CA1 epileptiform activity induced in rat hippocampal slices by kainic acid. The effects of the kynurenic acid derivatives 7-chlorokynurenic acid and 5-7 dichlorokynurenic acid and of the N...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00251-2
更新日期：1996-05-24 00:00:00
abstract：AIMS:Macrophage-derived foam-cell formation plays a crucial role in the development of atherosclerosis, and liver X receptor alpha (LXRα) is a key regulator of lipid metabolism in macrophages. Homocysteine (Hcy) is an independent risk factor of atherosclerosis; however, the regulation of lipid metabolism and role of LX...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.10.060
更新日期：2018-12-01 00:00:00
abstract：AIMS:Tianeptine is an atypical antidepressant drug that has a different mechanism of action than other antidepressants. Olanzapine is an atypical antipsychotic drug used for the treatment of schizophrenia. The present study was undertaken to investigate effects of chronic administration of tianeptine or olanzapine on u...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.023
更新日期：2012-12-17 00:00:00
abstract：:In saturation binding experiments, (+)pentazocine, (+)3-(3-hydroxyphenyl)-N-propylpiperidine (3-PPP), haloperidol and rimcazole did not inhibit the binding of [3H]DTG in a purely competitive fashion. Although Scatchard analysis indicated that [3H]DTG bound to a single site, the inhibition curves of some, but not all, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90255-n
更新日期：1992-01-01 00:00:00
abstract：:In our previous studies, exposure of mice to millimeter waves (MW) increased the duration of anesthesia caused by either ketamine or chloral hydrate, and this effect was blocked by naloxone. To further characterize the biological effects of MW, we have chosen a new animal model of experimental itch. Male Swiss albino ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00436-6
更新日期：1998-01-01 00:00:00
abstract：AIMS:Insulin receptor signaling in osteoblasts has been well established, but the effects of insulin on osteoclast proliferation are poorly explored. The objective of this study was to investigate the roles and the mechanisms of insulin on osteoclast proliferation. MAIN METHODS:After insulin treatment to primary osteo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.07.006
更新日期：2014-10-02 00:00:00
abstract：:It has been suggested that dopamine might play a role in the regulation of the immune system. In this study, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the expression of mRNA for the different subtypes of dopamine receptors in the rat lymphocytes. D1, D3 and D5 receptor mRNAs were...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00160-x
更新日期：1998-01-01 00:00:00
abstract：:Extracts of Chan Su, a traditional Chinese medication used as a topical anesthetic and cardiac medication, were incubated with cardiomyocytes that had been loaded with a calcium specific fluorescent probe. Calcium transients were measured by real-time fluorescence spectrophotometry following treatment. The transients ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02302-0
更新日期：2002-12-27 00:00:00
abstract：AIM:This study was undertaken to examine the effect of cyclooxygenase (COX) 2 inhibition on the development of muscular insulin resistance in high-fat-induced obese rats. MAIN METHODS:The rats were on a regular chow diet (C) or high-fat enriched diet (HFD) energy-restrictedly (HFr), or ad libitum (HFa) for 12weeks. Th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.05.006
更新日期：2011-07-18 00:00:00
abstract：AIMS:The aim of the present study was to evaluate the oral resveratrol effects associated with diet and physical training changes on anthropometric and biochemical parameters. MAIN METHODS:25 individuals aged from 30 to 60 years old; with Body Mass Index (BMI) ≥ 30 kg/m2 were included in the study. Following the prima...
journal_title：Life sciences
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.lfs.2020.117962
更新日期：2020-09-01 00:00:00
abstract：:We have confirmed that hypoxia elicits a substantial rise in blood choline levels in young adult rats. An intravenous infusion of tracer quantities of [2H4]-Ch, serial measurements of blood [2H0]-Ch and [2H4]-Ch, and a simple pharmacokinetic model were used to assess the bidirectional flux of choline between the centr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00191-9
更新日期：1996-01-01 00:00:00
abstract：:A recent neuropathological study has reported decreased levels of dynorphin A immunoreactivity in striato-pallidal fibers in the brain of a patient with severe Gilles de la Tourette's syndrome (TS). This observation, taken with the neuroanatomic distribution of dynorphin and its broad range of motor and behavioral eff...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90575-9
更新日期：1988-01-01 00:00:00
abstract：:Three distinct peptidyldipeptidases (exopeptidases releasing carboxyl terminal dipeptide residues) can be solubilized from nerve terminal membrane fractions from whole rat brain or striatum, and separated by ion exchange chromatography. Brain angiotensin-converting enzyme (PDP-1) cleaves Hip-His-Leu, but not 80 nM [3H...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90435-6
更新日期：1983-01-01 00:00:00
abstract：AIMS:Cold stress has been shown to produce dramatic increases in 2-fluoro-2-deoxy-D-Glucose ((18)FDG) accumulation by brown adipose tissue (BAT) in rodents. However, neither the effects of other types of stress on (18)FDG accumulation nor the effects of stressors on the accumulation of tracers of other aspects of energ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.04.014
更新日期：2011-07-18 00:00:00
abstract：AIMS:Studies indicate that the pattern of shear stress determines the direction of endothelial progenitor cells (EPCs) differentiation. However, the mechanism remains largely unknown. Herein, we try to identify the role of oscillatory shear stress (OSS) in the transdifferentiation of EPCs into mesenchymal cells and the...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117728
更新日期：2020-07-15 00:00:00
abstract：:Time-course studies revealed the increased susceptibility of the gastric mucosa to noxious injury in portal hypertension correlates with the level of elevated portal venous pressure and hyperglucagonemia. Whether acute elevation of portal venous pressure by exogenous glucagon aggravates such injury is not known. We te...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00413-2
更新日期：2003-07-18 00:00:00
abstract：AIMS:Individual susceptibility to develop obesity may impact the development of cardio-metabolic risk factors that lead to obesity-related comorbid conditions. Obesity-prone Osborne-Mendel (OM) rats expressed higher levels of visceral adipose inflammation than obesity-resistant, S5B/Pl (S5B) rats. However, the consumpt...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.03.022
更新日期：2019-04-15 00:00:00
abstract：:The role of endothelium-derived nitric oxide (NO) to cause smooth muscle phospholamban (PLB) phosphorylation was studied in the isolated perfused rat aorta precontracted with norepinephrine using a back-phosphorylation technique. NO-induced relaxation was associated with increased PLB-phosphorylation while norepinephr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90357-u
更新日期：1992-01-01 00:00:00
abstract：:The present study was to test whether the recently described endogenous ligand for the cannabinoid receptor; arachidonyl-ethanolamide (anandamide, ANA), may produce similar effects on pregnancy as the main psychoactive component of marihuana: delta9-tetrahydrocannabinol (THC) in rats. ANA, THC (0.02 mg/kg i.p./day, re...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00296-8
更新日期：1997-01-01 00:00:00
abstract：AIM:Preparation of pegylated gold nanorods (PEG-AuNRs) that are capable of converting near infrared (NIR) light into heat. Evaluation of cancer therapeutic efficacy and long-term toxicity of the proposed photothermal therapy in comparison with other conventional modalities. MATERIALS AND METHODS:Prepared PEG-AuNRs wer...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118108
更新日期：2020-09-15 00:00:00
abstract：BACKGROUND/AIMS:Previous studies have demonstrated that Galactooligosaccharides (GOS), known as "bifidus factor", has anti-inflammatory effects. Colitis, a kind of colonic inflammatory damage could be induced by different chemicals. The pathogenesis and mechanism of colitis remains unclear, and may be related to intest...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117220
更新日期：2020-02-01 00:00:00
