Abstract:
:Linear sucrose density gradient centrifugation of a crude synaptosomal-mitochondrial preparation of rat striatum was performed at 82,500g for 7.5, 15 and 30 min and 1, 4 and 20 h. After centrifugation various marker enzyme activities were measured throughout the gradients, viz. tyrosine hydroxylase (TH) and DOPA decarboxylase (DD) as markers of dopaminergic synaptosomes, lactate dehydrogenase (LDH) as a general synaptosomal marker and monoamine oxidase (MAO) as a mitochondrial marker. At all centrifugation times the distribution patterns of TH and DD activity coincided almost perfectly. Notable differences were found between the sedimentation properties of these TH/DD-containing particles and LDH-containing particles: TH and DD were symmetrically distributed in the gradient much sooner than LDH, at all centrifugation times the top of the TH and DD curves was lying deeper in the gradient than the highest LDH activity, and TH and DD became enriched in the gradients to a much greater extent than LDH. It is concluded that rat striatal dopaminergic synaptosomes form a relatively homogeneous population of particles sedimenting faster into the gradients than the bulk of striatal synaptosomes does. This distinct sedimentation behaviour of the dopaminergic synaptosomes can be usefully applied for analytical purposes.
journal_name
Life Scijournal_title
Life sciencesauthors
Van der Krogt JA,Koot-Gronsveld E,Van den Berg CJdoi
10.1016/0024-3205(83)90248-5subject
Has Abstractpub_date
1983-08-15 00:00:00pages
605-13issue
7eissn
0024-3205issn
1879-0631pii
0024-3205(83)90248-5journal_volume
33pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:OBJECTIVES:To investigate the effects of lipoxin A4 (LXA4) on inflammatory responses in obesity-related glomerulopathy (ORG) mouse model and its potential mechanisms. METHODS:Male C57BL/6 mice were randomly divided into 4 groups: normal, model, LXA4, and LXA4/Boc-2 groups (n = 8). Mice in LXA4 group were intraperitone...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.02.039
更新日期:2018-04-01 00:00:00
abstract::The concentration of epinephrine, norepinephrine, dopamine, met-enkephalin-, ACTH-, calcitonin- and somatostatin-like immunoreactivity (IR) were determined in the extracts of 9 adrenal pheochromocytomas from 7 patients. Six of these patients had Sipple's syndrome. There was a close correlation between the amounts of m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90652-0
更新日期:1984-07-23 00:00:00
abstract::The effect of changes in carotid sinus perfusion pressure on plasma immunoreactive atrial natriuretic peptide (IR-ANP) was examined in anaesthetized rabbits, and the role of arterial pressure in mediating the changes in IR-ANP was assessed. Plasma IR-ANP was significantly greater (101.7 +/- 24.3 pg ml-1) when carotid ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90355-x
更新日期:1989-01-01 00:00:00
abstract:AIMS:Endurance exercise causes fatigue due to mitochondrial dysfunction and oxidative stress. In order to find an effective strategy to prevent fatigue or enhance recovery, the effects of a combination of mitochondrial targeting nutrients on physical activity, mitochondrial function and oxidative stress in exercised ra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.11.003
更新日期:2010-01-02 00:00:00
abstract::Daily s.c. injection of (-)deprenyl (2.0 mg/kg/day) for three weeks in young male rats caused a threefold increase in superoxide dismutase (SOD) activity in the striatum of the brain compared with the value in saline-injected control rats. Furthermore, the activity of catalase (but not of glutathione peroxidase) was a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90466-o
更新日期:1991-01-01 00:00:00
abstract::Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by symptoms that include social communication impairments, interaction deficits, and repetitive and stereotyped behaviors. Recent studies have suggested that imbalanced cytokine levels are associated with impaired behavioral outcomes in ind...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116930
更新日期:2019-11-15 00:00:00
abstract::This study was essentially an in vivo protection experiment designed to test further the hypothesis that stress induces release of endogenous opioids which then act at opioid receptors. Rats that were either subjected to restraint stress for 1 hr or unstressed were injected ICV with either saline or 2.5 micrograms of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90430-9
更新日期:1987-12-28 00:00:00
abstract::The [R] and [S] enantiomers of the enkephalinase A inhibitor [R,S]-thiorphan have been prepared by asymmetric synthesis. The [S] isomer is principally responsible for the angiotensin converting enzyme inhibitory activity of [R,S]-thiorphan, whereas there were only small differences in the ability of the [R] and [S] is...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90277-2
更新日期:1985-04-01 00:00:00
abstract:AIMS:Constant development of chemotherapeutic strategies has considerably improved the efficiency of tumor treatment. However, adverse effects of chemotherapeutics enforce premature treatment cessation, which leads to the tumor recurrence and accelerated death of oncologic patients. Recently, sodium ascorbate (ASC) has...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116657
更新日期:2019-09-01 00:00:00
abstract::Physiological, pharmacological and toxicological responses to two regimens of cocaine administration were compared between spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. An initial experiment examined renal excretory and hemodynamic function in response to an acute volume load in anesthetized SHR and WK...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90254-3
更新日期:1989-01-01 00:00:00
abstract::Although effects of a metastasis suppressor gene, KiSS-1, have been postulated to be mediated by its receptor, hOT7T175, the mechanism of such effects remains unknown. This study was designed to evaluate the mechanism of how KiSS-1 works and to assess effects of a synthesized truncated KiSS-1 protein on the invasive a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.06.018
更新日期:2008-08-29 00:00:00
abstract::[3H]5-HT binding sites were analyzed in membranes prepared from the rabbit caudate nucleus (CN). [3H]5-HT labeled both 5-HT1A and 5-HT1C recognition sites, defined by nanomolar affinity for 8-OH-DPAT and mesulergine respectively; however, these represented only a fraction of total specific [3H]5-HT binding. Saturation...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90033-7
更新日期:1989-01-01 00:00:00
abstract::The hypothalamic AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) pathway is known to play an important role in the control of food intake and energy expenditure. Here, we hypothesize that citrate, an intermediate metabolite, activates hypothalamic ACC and is involved in the control of energy mobilizat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.04.015
更新日期:2008-06-20 00:00:00
abstract::We have evaluated the effect of intraperitoneal (I.P.) injection of human recombinant interferon-2alpha (IFN-alpha) on Bleomycin-induced pulmonary injury in hamsters. Pulmonary injury was induced by a single intratracheal (I.T.) instillation of Bleomycin (Bleo). Six groups of male Syrian hamsters were treated as follo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00332-9
更新日期:1997-01-01 00:00:00
abstract::Using a dipole tracing method based on the two-dipole model, the purpose of the present study was to investigate alcohol-induced changes in the alpha band of electroencephalogram (EEG) and its equivalent current dipoles (ECDs) in 12 healthy male volunteers, who were genetically typed for mitochondrial aldehyde dehydro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00707-4
更新日期:2000-01-01 00:00:00
abstract::Viperid, elapid and crotalid snake venoms were screened in vitro for antiviral activity against Sendai virus. The hemolysis of 10(8) human erythrocytes in 1 ml, caused by 70 HAU of Sendai virus, was abolished when the virions were pretreated with 10 ug of the viperid venom of Echis coloratus, and was considerably dimi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90015-h
更新日期:1992-01-01 00:00:00
abstract::We report the presence of p-fluorophenylglycine (p-FPG) in the urine of six baboons treated with HPTP, the tetrahydropyridine dehydration product of haloperidol (HP). Oxidative N-dealkylation, the major metabolic pathway of HP, gives rise to 3-(4-fluorobenzoyl)propionic acid (p-FBPA). Subsequent beta-oxidation of p-FB...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00274-x
更新日期:1999-01-01 00:00:00
abstract::This report documents studies on the spontaneous reactivation of human erythrocyte acetylcholinesterase and human serum butyrylcholinesterase following inhibition by organophosphinate esters. The spontaneous reactivation reactions were carried out at 26.0 degrees C in 0.10 M phosphate buffer of pH 7.6. Based upon resu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90553-3
更新日期:1985-05-06 00:00:00
abstract::Sensitivity of brain muscarinic acetylcholine receptors to the agonists was examined in nicotine tolerant animals which were developed by acutely repeated injections of nicotine. In conscious rats, the dose-response curves of muscarinic agonists arecoline and pilocarpine, cholinesterase inhibitors soman and physostigm...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00469-9
更新日期:1996-01-01 00:00:00
abstract::The effects of (-)-epigallocatechin-3-gallate (EGCG), the most abundant catechin of tea, on Ca(2+)-permeable non-selective cation currents (NSCC) and voltage-operated Ca(2+) channels (VOCC) have been investigated in cultured rat aortic smooth muscle cells using the whole-cell voltage-clamp technique. Under the Cs(+)/t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.04.005
更新日期:2007-05-16 00:00:00
abstract::It is well known that the immune function can be compromised by stress. To investigate immune function in mice stressed by experimental restraint or unavoidable and opioid dependent stress, we evaluated the changes in total body weight and in organ weights (liver, spleen and thymus) of these animals, as well as the ph...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90600-v
更新日期:1990-01-01 00:00:00
abstract::Proteinase inhibitors were tested for their ability to inhibit prolactin (PRL) and growth hormone (GH) release by cultured anterior pituitary cells of the rat. Inhibitors of microbial origin (chymostatin, elastatinal, leupeptin) had either no or a moderate effect on hormone release while some tripeptide aldehydes, esp...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90636-8
更新日期:1985-02-11 00:00:00
abstract::Male Sprague-Dawley rats were castrated and given daily sc. injections of estradiol (E2, 10 micrograms/day), testosterone propionate (TP, 1.0 mg/day), dihydrotestosterone (DHT, 1.0 mg/day) or sesame oil (SO, 0.2 ml/day). A group of sham castrate males received daily sc. injections of SO (0.2 ml/day). On day 8 of stero...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90327-8
更新日期:1984-01-02 00:00:00
abstract::Arginine vasotocin has been proposed as a hormone of the mammalian pineal gland. This claim is largely based on biological and immunological results, although some chemical evidence has also been provided. Yet, more and more researchers have recently disclaimed the presence of this nonapeptide in mammalian tissue. The...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(84)90267-4
更新日期:1984-10-22 00:00:00
abstract::This study examined the protective effect of phenobarbital on kainic acid-induced deficits in acquisition learning. A single kainic acid injection (9 mg/kg i.p.) was administered five days prior to testing using the Morris water maze test. Kainic acid produced deficits in the acquisition of spatial information observe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00658-5
更新日期:2000-06-30 00:00:00
abstract::It was demonstrated in the previous study that the microinjection of antisense oligodeoxynucleotide (AS ODN) against mu-opioid receptor (MOR) into periaqueductal gray (PAG) of rat brain selectively decreased the MOR mRNA content in PAG, and the decrease in MOR mRNA content was enhanced by pretreatment of the PAG with ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01009-8
更新日期:2001-04-06 00:00:00
abstract::Chymase is an important enzyme for the generation of angiotensin (Ang) II and in the activation of transforming growth factor (TGF)-beta1. Therefore, chymase may be involved in the hemodialysis access dysfunction, which is caused by intimal hyperplasia that occurs after polytetrafluoroethylene (PTFE) graft implantatio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.09.004
更新日期:2007-09-29 00:00:00
abstract::We studied the incorporation of 14C-formate into uric acid and allantoin in different organs (liver, lung, kidney, spleen), isolated hepatocytes, perfused liver and urine of the rat. Allantoin had a higher specific radioactivity than uric acid after 14C-formate load in the liver in vivo. This was found to be a strictl...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00387-6
更新日期:1996-01-01 00:00:00
abstract::In a series of in vitro experiments we characterised the relationship between DNA distribution in the G1, S and G2/M phases of cell cycle and PDE and GST activity in CaCo-2 cells. The DNA distribution in CaCo-2 cells, was assessed by flow cytometry, with fluorescent dyes at different time points of culture. The expone...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00169-7
更新日期:1995-01-01 00:00:00
abstract::We studied the effects of cAMP on cytokine (interferon-gamma plus tumor necrosis factor-alpha)-induced stimulation of tetrahydrobiopterin (BH4) synthesis in human umbilical vein endothelial cells (HUVEC). The cytokine mixture caused a marked increase in the biosynthesis and release of BH4 by HUVEC. Dibutyryl-cAMP prod...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01503-5
更新日期:2002-03-22 00:00:00