Abstract：
:In a series of in vitro experiments we characterised the relationship between DNA distribution in the G1, S and G2/M phases of cell cycle and PDE and GST activity in CaCo-2 cells. The DNA distribution in CaCo-2 cells, was assessed by flow cytometry, with fluorescent dyes at different time points of culture. The exponential increase in cell number continued until day 10 when there was cell saturation. The effect of medium replacement on PDE activity was assayed in the first 10 h after medium replacement. The 6th hour is the time at which PDE activity was found to be highest. We have assayed the PDE enzyme with cGMP and cAMP as substrates. Only cAMP was consumed from this enzyme. We found a very close correlation between the DNA distribution in the various phases of the cell cycle and the PDE activity. PDE activity was very high during the active replication phase, whereas GST activity was high after confluency.
journal_name
Life Scijournal_title
Life sciencesauthors
Savini F,Berardi S,Tatone D,Spoto Gdoi
10.1016/0024-3205(95)00169-7subject
Has Abstractpub_date
1995-01-01 00:00:00pages
PL421-5issue
22eissn
0024-3205issn
1879-0631pii
0024320595001697journal_volume
56pub_type
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