Abstract:
AIMS:To identify the target of an adipose specific aptamer adipo-8, predict the potential interaction between adipo-8 and its target, and investigate lipid-lowering effect of adipo-8 in vitro and in vivo. MAIN METHODS:Distinct membranous protein of 3T3-L1 adipocyte pulled-down by adipo-8 was mass-spectrometry analyzed as target candidate(s), and affinity of adipo-8 to target protein-silent adipocyte was detected to validate it. Interaction between adipo-8 and target was predicted by bioinformatic analysis, further confirmed by aptamer truncation and competitive binding assay. To investigate lipid-lowering effect of adipo-8 and mechanism behind, 250 nmol/L adipo-8 or library was incubated with 3T3-L1 adipocyte or target-protein-silent adipocyte for 24 h, and 0.01 μg/g/day adipo-8 or library was administrated to high-fat-fed male mice for 21 days. KEY FINDINGS:APMAP (Adipocyte Plasma Membrane Associated Protein) was identified as adipo-8 target, and adipo-8 affinity to adipocytes was in proportional to APMAP expression. Docking model between the stem-loop structure of adipo-8 and APMAP were predicted that adipo-8 was likely to interact with APMAP at its amino-acid 275-411 sequence. Moreover, adipo-8 could ameliorate fat deposition through interaction with APMAP in vitro, and administration of adipo-8 in high-fat-diet fed mice resulted in body weight loss and blood triglyceride decrease without liver or renal dysfunction. SIGNIFICANCE:Adipo-8 could recognize APMAP specifically and interact with its targets to ameliorate fat deposition in vitro and in vivo. Aptamer adipo-8 has potential to act as an effective and safe targeted drug for obesity and obesity related diseases.
journal_name
Life Scijournal_title
Life sciencesauthors
Zhong W,Zhang Y,Tan W,Zhang J,Liu J,Wang G,Liao J,Liu B,Chen K,Yu B,Deng Y,Zou Y,Pu Y,Liu Hdoi
10.1016/j.lfs.2020.117609subject
Has Abstractpub_date
2020-06-15 00:00:00pages
117609eissn
0024-3205issn
1879-0631pii
S0024-3205(20)30357-Xjournal_volume
251pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::An enzyme partially purified from bovine lung membranes appears to be endothelin converting enzyme (ECE). This enzyme specifically cleaves big endothelin-1 (big ET-1) at the proper site, between Trp21 and Val22, with maximum activity at pH 7.5 and with a Km of roughly 3 microM, to produce endothelin-1 (ET-1) and C-ter...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90175-o
更新日期:1992-01-01 00:00:00
abstract::Vanilloid spice principles, including capsaicin, stimulate vasoconstriction in the rat hindlimb perfused at constant flow and, depending on dose, either stimulate or inhibit oxygen consumption by this vascular bed. We now present metabolic and functional evidence for two different vanilloid (VN1 and VN2) receptor type...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00250-a
更新日期:1995-01-01 00:00:00
abstract::Somatostatin (SS) acts as a universal endocrine off-swich, and also inhibits the growth of neuroendocrine tumors through its specific receptors. Small cell lung cancer (SCLC) demonstrates some neuroendocrine characteristics and has been proposed as a candidate for treatment with SS and its analogues. In the present st...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90090-6
更新日期:1994-01-01 00:00:00
abstract::High performance liquid chromatography combined with either single quad or triple quad mass spectral detectors (LC/MS) was used to measure the brain distribution of receptor occupancy tracers targeting dopamine D2, serotonin 5-HT2A and neurokinin NK-1 receptors using the ligands raclopride, MDL-100907 and GR205171, re...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.075
更新日期:2005-12-12 00:00:00
abstract:AIMS:A growing body of evidence shows the cardiovascular benefits of fish oil ingredients, including eicosapentaenoic acid (EPA), in humans and experimental animals. However, the effects of EPA on endothelin (ET)-1-induced cardiomyocyte hypertrophy and the involved signaling cascade are largely unknown. A previous stud...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.04.025
更新日期:2014-11-24 00:00:00
abstract::The mechanism of the generation and the quantitative analysis of singlet oxygen (1O2) formed by the exposure of a hematoporphyrin derivative (HpD) to light was re-evaluated by electron spin resonance (ESR) combined with 2,2,6,6,-tetramethyl-4-piperidine (TMPD). The change from TMPD to 2,2,6,6,-tetramethyl-4-piperidine...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00835-7
更新日期:1997-01-01 00:00:00
abstract::1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-O-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90129-9
更新日期:1987-04-20 00:00:00
abstract::Angiogenesis-targeted therapy of cancer is considered a promising strategy for therapeutic management of cancer progression. Over the last two decades, a few anti-angiogenesis monoclonal antibodies (mAbs) blocking VEGF signaling have been developed and approved by the FDA. The most widely used anti-angiogenesis drug i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117018
更新日期:2019-12-15 00:00:00
abstract:AIMS:The purpose of this study was to determine a relatively short-term effect of feeding an α-linolenic acid (ALA, n-3)-restricted, linoleic acid (LA, n-6)-adequate diet on neurotrophin contents and protein kinase activities in brain regions of the mouse. MAIN METHODS:After feeding mice a safflower oil (SAF) diet (AL...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.003
更新日期:2010-10-09 00:00:00
abstract::Respiring mitochondria represent the major source of superoxide production in most cells, and superoxide anions function as direct precursors of hydrogen peroxide formation within mitochondria. We use a lucigenen-derived chemiluminescence (LDCL) assay to test the hypothesis that intramitochondrial superoxide productio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01538-2
更新日期:2002-05-03 00:00:00
abstract::In present study the methylotrophic yeast, Pichia pastoris, was used to express a recombinant growth hormone (rGH) gene of swine. A synthetic secretion cassette was constructed using the promoter of the alcohol oxidase1 gene (AOX1), and a alpha-factor signal peptide. After electroporatic transformation and zeocin sele...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00797-9
更新日期:2000-09-15 00:00:00
abstract::Clinical trials with muscarinic agonists or acetylcholine esterase inhibitors for the treatment of Alzheimer's dementia have shown disappointing or equivocal results. An alternative treatment of this disease is the development of drugs which enhance the release of acetylcholine. It is believed, that of the five muscar...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(93)90307-o
更新日期:1993-01-01 00:00:00
abstract::Inert gas narcosis is a neurological syndrome appearing when humans or animals are exposed to hyperbaric inert gases (nitrogen, argon) composed by motor and cognitive impairments. Inert gas narcosis induces a decrease of the dopamine release at the striatum level, structure involved in the regulation of the extrapyram...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00183-8
更新日期:2003-05-02 00:00:00
abstract:AIMS:We tested the hypothesis that respiratory quotient (RQ) determines sympathovagal balance associated with metabolism of stored and dietary energy substrates. MAIN METHODS:Six 18-20 year-old African-American males were studied after two control pretreatments of fasting and post-treatments of metabolizing high-fat a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.05.006
更新日期:2009-07-17 00:00:00
abstract::Endothelins (ET) are 21-amino acid peptides that bind to membrane receptors to initiate a wide range of pathophysiological effects. PD-156707, L-749329, Ro-470203, and A-127722 are potent non-peptide ET receptor antagonists developed recently. When tested in human and rat plasma, both ET-1 and -3 and the four aforemen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00168-3
更新日期:1996-01-01 00:00:00
abstract::The effects of the benzodiazepines diazepam and midazolam on the rigidity produced by systemic administration of morphine (15 mg/kg i.p.) were studied in rats. The rigidity was recorded as a tonic activity from the gastrocnemius-soleus muscle of non-anesthetized rats in the electromyogram. Both diazepam (1-5 mg/kg i.p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90612-4
更新日期:1983-01-01 00:00:00
abstract::The influence of the stage of the estrous cycle on binding of [3H]diazepam was examined in membranes from brains of female mice. In order to conserve endogenous factors such as progesterone, other steroids, or GABA, the assay was performed without the extensive washing procedures typically employed in measurements of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02177-k
更新日期:1995-01-01 00:00:00
abstract::A completely randomized controlled study based on a rabbit model was designed to study the effect of repetitive ischemic preconditioning (IPC) on a spinal cord ischemic reperfusion injury. Twenty four white adult Japanese rabbits were randomly assigned to one of the 3 groups (n = 8 per group): Group I: sham-operation ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.04.014
更新日期:2006-09-05 00:00:00
abstract::In the present study, we investigated the effects of calmodulin, adenosine 5'-triphosphate (ATP) and pertussis toxin (PT) on phorbol ester (PMA) (a protein kinase C activator) induced inhibition of ANF-stimulated cyclic GMP formation in cells from the human renal cell line, SK-NEP-1. PMA inhibited ANF-stimulated guany...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90508-9
更新日期:1991-01-01 00:00:00
abstract::Benzodiazepines reliably produce overconsumption of food and fluids. Opiate antagonists, naloxone and naltrexone, block the benzodiazepine-induced hyperphagia and hyperdipsia at low doses. Hence, activation of endogenous opioid mechanisms may be closely involved in the benzodiazepine facilitatory effects on ingestiona...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(83)90108-x
更新日期:1983-03-07 00:00:00
abstract:AIMS:To investigate the roles of neutrophil alkaline phosphatase (NAP) in the migration, reactive oxygen species (ROS) generation and apoptosis of neutrophils. MAIN METHODS:NAP was overexpressed in neutrophil-like differentiated HL-60 cells via transfecting coding sequence of NAP by lentivirus. NAP overexpression in H...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.06.005
更新日期:2016-07-15 00:00:00
abstract::Although lipid peroxidation of biological samples may be assessed by different chemical and physical methods, those based on the measurement of malondialdehyde formed from the breakdown of endoperoxides during the last stages of the oxidation of a polyunsaturated fatty acid, appear as the most widely used. Among the v...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(91)90550-u
更新日期:1991-01-01 00:00:00
abstract::Repeated, low-intensity electrical stimulation of the heart gradually leads to the development of electrocardiographic abnormalities that culminate in cardiac arrhythmias, mainly A-V block in the isolated heart frog and ventricular and supraventricular tachyarrhythmias in the canine heart in situ. The pattern of devel...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90437-7
更新日期:1988-01-01 00:00:00
abstract:AIMS:Short-chain acyl-CoA dehydrogenase (SCAD) is a key enzyme in fatty acid oxidation. In the present study we aim to investigate the changes in SCAD between pathological and physiological cardiomyocyte hypertrophy. We also explore the different signaling pathways of pathological and physiological cardiomyocyte hypert...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.01.015
更新日期:2015-03-01 00:00:00
abstract::Uric acid values in serum have been analyzed as one of the markers to predict cellular damage due to ischemia reperfusion injury in the field of organ transplantation. The present study was conducted to confirm that uric acid values in serum could be an efficient marker of ischemic injury of liver parenchyma following...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00566-9
更新日期:1999-01-01 00:00:00
abstract::The objective of this study was to investigate whether age induces changes on vasodilator response induced by cromakalim, an ATP-sensitive K+ (K(ATP)) channel opener, as well as the underlying mechanism involved in this possible alteration. For this purpose, aortic segments from young (3-5 months) and old (3 years) ra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)80003-4
更新日期:1998-01-01 00:00:00
abstract:AIMS:Osteoclasts, the unique bone-resorbing polykaryons, are responsible for many bone-destructive diseases, such as osteoporosis and rheumatoid arthritis. Hence, the regulation of osteoclast formation is considered a potential therapeutic approach for these diseases. In this study, we investigated the effect of a nove...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.09.009
更新日期:2012-11-02 00:00:00
abstract::Endothelin-1 (ET-1) is produced from its precursor, big endothelin-1 (BigET-1), by a putative endothelin-converting enzyme (ECE), but it is not known whether the enzyme is present in the brain. This study was conducted to examine the central hemodynamic effects of BigET-1 and to indirectly determine the presence of an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90491-s
更新日期:1991-01-01 00:00:00
abstract::Morning urine samples were assayed for human EGF (hEGF) in 137 non-insulin dependent diabetic patients with normal serum creatinine and beta 2-microglobulin levels. Serving as controls, 80 age- and sex-matched healthy subjects were also examined. A significant positive correlation between hEGF excretion and the level ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90100-h
更新日期:1993-01-01 00:00:00
abstract::The purpose of this study is to report the development of a non-radioactive fluorescent peptide assay for measuring protein kinase C activity (PKC). The assay is based on a glycogen synthase derived fluorescent peptide that is phosphorylated by PKC. Phosphorylation causes the peptide to migrate toward the anode while ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02149-d
更新日期:1995-01-01 00:00:00