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Critical role of neutrophil alkaline phosphatase in the antimicrobial function of neutrophils.

Abstract：

AIMS:To investigate the roles of neutrophil alkaline phosphatase (NAP) in the migration, reactive oxygen species (ROS) generation and apoptosis of neutrophils. MAIN METHODS:NAP was overexpressed in neutrophil-like differentiated HL-60 cells via transfecting coding sequence of NAP by lentivirus. NAP overexpression in HL-60 cells was confirmed by the methods of quantitative RT-PCR and Western blotting. HL-60 cells were induced to differentiate into neutrophil-like cells by exposure to 1.5% dimethylsulfoxide (DMSO). The migration of neutrophil-like cells were detected by Transwell migration assay. ROS generation of neutrophil-like cells were determined by flow cytometry. Neutrophil-like cells continued to be cultured for 24h, and were then harvested for apoptosis and Western blotting. KEY FINDING:After GFP-NAP infection by lentivirus, the expression of NAP was up-regulated in HL-60 cells. HL-60 cells were allowed to differentiate into neutrophil-like cells after 5-day exposure to 1.5% DMSO. Overexpression of NAP in neutrophil-like cells resulted in an increase in the number of migrated cells, intracellular ROS and cell apoptosis followed by a rise in the expression of Caspase 3, Caspase 9 and Bax, while those results were reversed in the NEG and CON group. SIGNIFICANCE:NAP might play a critical role in the anti-microbial function of neutrophils by promoting its migration and ROS generation, as well as accelerating apoptosis in neutrophils.

journal_name

Life Sci

journal_title

Life sciences

authors

Li H,Zhao Y,Li W,Yang J,Wu H

doi

10.1016/j.lfs.2016.06.005

subject

Has Abstract

pub_date

2016-07-15 00:00:00

pages

152-157

eissn

0024-3205

issn

1879-0631

pii

S0024-3205(16)30351-4

journal_volume

157

pub_type

杂志文章

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