Abstract:
:Aberrant methylation of promoter CpG islands (CGIs) can inactivate the expression of many tumor suppressor genes and play an important role in the carcinogenesis of gastric cancer. The tumor suppressor gene RB1, which encodes a cell cycle regulator, is hypermethylated and downregulated in multiple kinds of cancer. Activation of RB1 expression through DNA demethylation is a potential strategy for the treatment of gastric cancer. Herein, we found that the methylation status of the RB1 promoter was negatively related to the development of gastric tumors, while its expression was positively correlated with TET2 and TET3 expression. Further reactivation of RB1 expression by curcumin could inhibit gastric cell viability and carcinogenesis both in vitro and in vivo. Molecular docking and other studies confirmed that curcumin could bind to and upregulate the expression of TET2 and TET3 with hydrogen bonds and arene-H bonds, suggesting that demethylation of RB1 was attributed to reactivation of the demethylation enzymes TET2 and TET3 after curcumin treatment. Thus, our findings reveal a promising therapeutic strategy for gastric cancer prevention and treatment through RB1 demethylation and reactivation.
journal_name
Life Scijournal_title
Life sciencesauthors
Cao D,Jia Z,Wu Y,Su T,Zhao D,Wu M,Tsukamoto T,Oshima M,Jiang J,Cao Xdoi
10.1016/j.lfs.2020.118580subject
Has Abstractpub_date
2020-12-15 00:00:00pages
118580eissn
0024-3205issn
1879-0631pii
S0024-3205(20)31333-3journal_volume
263pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:Intestinal alkaline phosphatase (IAP) is an intestinal brush border enzyme that is shown to function as a gut mucosal defense factor, but its defensive mechanism remains unclear. The aims of this study were to evaluate the effect of IAP on intestinal epithelial cells and macrophages, and on chronic colitis in inte...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.02.003
更新日期:2014-04-01 00:00:00
abstract::Intravenous injection of prostacyclin (100 micrograms/kg) in rats resulted in a decrease of systolic blood pressure within 2 minutes. Concentrations of cAMP in 15 brain regions and nuclei were determined by radioimmunoassay. In lower brain stem nuclei, such as the nucleus of the solitary tract and the lateral reticula...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90439-5
更新日期:1987-12-07 00:00:00
abstract::The hypothermia induced by apomorphine, a mixed dopamine (DA) agonist in male Swiss-Webster mice, was not blocked by the selective D-1 antagonist SCH 23390 but was completely blocked by the selective D-2 antagonists haloperidol, sulpiride and YM-09151-2. The selective D-1 agonist SKF 38393 did not elicit hypothermic r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90515-2
更新日期:1988-01-01 00:00:00
abstract::The renal hemodynamic and tubular effects of ANF were investigated using the Sperber technique in chickens. This technique takes advantage of the unique portal circulation of the avian kidney and permits direct access to the renal peritubular space independent of renal arterial blood flow and glomerular filtration. In...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90309-2
更新日期:1987-03-09 00:00:00
abstract::The effect of nimodipine on nitric oxide synthase (NOS) activities in brains in transient focal cerebral ischemia rats, in cultured mouse neurons and astroglial cells and bovine brain capillary endothelial cells (BCECs) was investigated. The administration of nimodipine (3 mg.kg(-1), p.o., twice a day, for 3 days) bef...
journal_title:Life sciences
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Human milk was tested in the Ames plate incorporation test using strain TA98. Pools from smokers and non-smokers respectively, as well as individual samples from 14 smokers and 15 controls were tested. No difference was found between milk from smokers and non-smokers in the concentrations and volumes used. Preliminary...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90663-1
更新日期:1987-11-23 00:00:00
abstract::The circadian rhythm of 6-sulphatoxymelatonin (aMT6s) excretion has been determined in male and female rats at 3 weeks and at 2, 8, 14 and 20 months of age. All animals have a pronounced circadian pattern of aMT6s excretion under a 12 hour dark: 12 hour light cycle. A significant increase in aMT6s excretion is observe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90323-h
更新日期:1992-01-01 00:00:00
abstract::The effect of clotrimazole on Ca2+ signaling in Madin Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ indicator. Clotrimazole (1-30 microM) induced a concentration-dependent [Ca2+]i increase. The [Ca2+]i increase comprised an initial rise and a slow decay. External Ca2+ removal partly inhib...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00558-0
更新日期:2000-01-01 00:00:00
abstract::11Beta-hydroxyglucocorticoids (HGCs) are known to induce apoptosis in immature T cells. Here we show that 11-oxoglucocorticoids (OGCs), which are oxidized metabolites of HGCs, counteract the apoptosis-inducing effects of HGC in murine thymocytes in vitro. Corticosterone at concentrations ranging from 0.1-100 microM in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01069-4
更新日期:2001-05-18 00:00:00
abstract::A modification of beta-adrenoceptor binding has been observed in different human and animal tissues after glucocorticoid administration. In this study we observed that hydrocortisone increased human intact lymphocyte beta-adrenoceptors labelled with 3H-dyhydroalprenolol in vitro, in physiological and pharmacological c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90528-9
更新日期:1984-12-03 00:00:00
abstract:AIMS:Insulin-like growth factor (IGF)-1 is a major mitogenic growth factor for mesangial cells (MCs). Statins slow the progression of chronic kidney disease by affecting inflammatory cell signaling pathways, in addition to improving lipid profile, however, no studies have investigated the effects of fluvastatin on mito...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.02.022
更新日期:2009-05-22 00:00:00
abstract::This paper gives a short account of the mechanisms regulating choline production. Although choline is absorbed from the GI tract and biosynthesized in the liver, its subsequent metabolism to choline esters and phospholipids seems to dominate in importance as to its regulatory role in maintaining a constant source of f...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(96)00181-6
更新日期:1996-01-01 00:00:00
abstract:AIM:This study was undertaken to determine the biological activity of a green tea supplement with respect to cells and erythrocyte membranes and the molecular mechanism of that activity. MAIN METHODS:The extract's activity was evaluated on the basis of its hemolytic, antioxidant and antiinflammatory actions. In additi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.12.025
更新日期:2015-04-01 00:00:00
abstract::Sodium-dependent transport of D-glucose has been reported only in epithelial cells of small intestine and kidney, and well-differentiated tumors thereof. We observed a two-fold decrease (p < 0.05) in the intracellular distribution volume (Vic, defined as steady-state intracellular uptake divided by extracellular conce...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00114-2
更新日期:1996-01-01 00:00:00
abstract::Rats administered cocaine 10 mg/kg i.p. exhibited hyperlocomotion which occurred within 5 min post injection. Subsequently, rats were injected with 10 mg/kg cocaine and brain and serum concentrations of cocaine were measured at 5, 10, and 20 min post injection. Within this time frame, cocaine concentration in limbic b...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00339-4
更新日期:1994-01-01 00:00:00
abstract::Fibrotic lung diseases qualify among the most dreaded irreversible interstitial pulmonary complications with progressive yet largely unpredictable clinical course. Idiopathic pulmonary fibrosis (IPF) is the most challenging prototype characterized by unknown and complex molecular etiology, severe dearth of non-invasiv...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118201
更新日期:2020-10-01 00:00:00
abstract::The α chain of interleukin 3 receptor (IL-3Rα or CD123), together with the common β (βc) subunit, forms a high-affinity IL-3R with biological function. In recent years, emerging research has found that CD123 is highly expressed on the surface of various cells (e.g., leukemia stem cells, LSCs), and it is associated wit...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2014.10.013
更新日期:2015-02-01 00:00:00
abstract::The purpose of this study was to investigate the effect of T3 on amino acid accumulation and on the membrane potential of Sertoli cells of immature rat testes. Testes of pre-pubertal and pubertal rats were pre-incubated (30 min) in Krebs-Ringer bicarbonate buffer and incubated in the presence of [14C]methylaminoisobut...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01186-9
更新日期:2001-07-13 00:00:00
abstract::Partially purified fractions from sheep pineal gland extracts, which show a fluorescence like pteridines, were shown to suppress the enzyme hydroxy-indole-o-methyltransferase (HIOMT) isolated from avian pineals and retinas. The results described demonstrate that the mechanism of inhibition of HIOMT activity is indepen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90022-4
更新日期:1982-04-19 00:00:00
abstract::Muscarinic agonists elicit contraction in the standard guinea pig ileum bioassay through activation of M3 muscarinic receptors that are also linked to phosphoinositide hydrolysis. Surprisingly, the most abundant muscarinic receptor in the ileum is the M2 which causes a specific inhibition of cyclic AMP accumulation el...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(94)00035-q
更新日期:1995-01-01 00:00:00
abstract::There is a growing literature indicating that opioid abuse by human addicts and opioid administration to animals have profound effects on the immune system. In the present study, implantation of morphine pellets in mice was associated with reduced phenotypic expression of the cell surface antigens specific to T-lympho...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90607-q
更新日期:1992-01-01 00:00:00
abstract:OBJECTIVE:A deficiency in hydrogen sulfide (H2S) and nitric oxide (NO) contributes to the development of type 2 diabetes (T2D). An inhibitory effect on liver gluconeogenesis has been reported in rats with T2D with co-administration of sodium nitrite and sodium hydrosulfide (NaSH); the underlying mechanisms have however...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118770
更新日期:2021-01-15 00:00:00
abstract:AIMS:This study analyzed in pithed rats the effect of several acute and prophylactic antimigraine drugs on the CGRPergic vasodepressor sensory outflow, in an attempt to investigate systemic cardiovascular effects in a model unrelated to migraine. MAIN METHODS:Male Wistar pithed rats were pretreated with continuous i.v...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.11.008
更新日期:2009-01-30 00:00:00
abstract::In rat brain microvessels, tetrahydroaminoacridine (THA) caused a significant inhibition of both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities, in a dose-dependent manner. THA resulted to be a more potent inhibitor of BuChE than AChE. Lineweaver-Burk plots showed that Km (app) and V were alt...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90553-f
更新日期:1993-01-01 00:00:00
abstract::The challenge of discovering new, urgently needed anti-TB drugs from natural sources requires a truly interdisciplinary research. Cutting-edge mycobacteriology and innovative natural products chemistry tools have to be developed and employed in tandem, in order to meet these demands. The present review provides cross-...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2005.09.004
更新日期:2005-12-22 00:00:00
abstract::An experimental model which resembles human drug addiction was developed to study the effect of chronic drug (cocaine or morphine) administration on the immune system. As malnutrition has been associated with drug use, a low protein diet has been evaluated for its contribution to the impairment of the immune system du...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90138-2
更新日期:1991-01-01 00:00:00
abstract::Nitric oxide (NO) is a key mediator in many physiological processes and one of the major receptors through which NO exerts its effects is soluble guanylyl cyclase. Guanylyl cyclase converts GTP to cyclic GMP as part of the cascade that results in physiological processes such as smooth muscle relaxation, neurotransmiss...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02361-5
更新日期:2003-01-17 00:00:00
abstract::Previous research has shown that the CAMK (calcium/calmodulin dependent protein kinase) inhibitor, KN62, can lead to reductions in insulin stimulated glucose transport. Although controversial, an L-type calcium channel mechanism has also been hypothesized to be involved in insulin stimulated glucose transport. The pur...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.06.041
更新日期:2004-01-02 00:00:00
abstract::The requirement for calcium in the serotonin-mediated aldosterone secretion was investigated using rat adrenal capsular cells. In the calcium-free medium both basal as well as serotonin-stimulated aldosterone secretion (at concentrations of 10(-7) M and 10(-8) M of serotonin) were significantly impaired. The effects o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90053-0
更新日期:1985-04-15 00:00:00
abstract::Diazepam binding inhibitor (DBI) is a 9-kD polypeptide that was first isolated in 1983 from rat brain by monitoring its ability to displace diazepam from the benzodiazepine (BZD) recognition site located on the extracellular domain of the type A receptor for gamma-aminobutyric acid (GABAA receptor) and from the mitoch...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(91)90440-m
更新日期:1991-01-01 00:00:00