Diazepam binding inhibitor (DBI): a peptide with multiple biological actions.


:Diazepam binding inhibitor (DBI) is a 9-kD polypeptide that was first isolated in 1983 from rat brain by monitoring its ability to displace diazepam from the benzodiazepine (BZD) recognition site located on the extracellular domain of the type A receptor for gamma-aminobutyric acid (GABAA receptor) and from the mitochondrial BZD receptor (MBR) located on the outer mitochondrial membrane. In brain, DBI and its two major processing products [DBI 33-50, or octadecaneuropeptide (ODN) and DBI 17-50, or triakontatetraneuropeptide (TTN)] are unevenly distributed in neurons, with the highest concentrations of DBI (10 to 50 microMs) being present in the hypothalamus, amygdala, cerebellum, and discrete areas of the thalamus, hippocampus, and cortex. DBI is also present in specialized glial cells (astroglia and Bergmann glia) and in peripheral tissues. In the periphery, the highest concentration of DBI occurs in cells of the zona glomerulosa and fasciculata of the adrenal cortex and in Leydig cells of the testis; interestingly, these are the same cell types in which MBRs are highly concentrated. Stimulation of MBRs by appropriate ligands (including DBI and TTN) facilitates cholesterol influx into mitochondria and the subsequent formation of pregnenolone, the parent molecule for endogenous steroid production; this facilitation occurs not only in peripheral steroidogenic tissues, but also in glial cells, the steroidogenic cells of the brain. Some of the steroids (pregnenolone sulfate, dehydroepiandrosterone sulfate, 3 alpha-hydroxy-5 alpha-pregnan-20-one, and 3 alpha, 21-dihydroxy-5 alpha-pregnan-20-one) produced in brain (neurosteroids) function as potent (with effects in the nanomolar concentration range) positive or negative allosteric modulators of GABAA receptor function. Thus, accumulating evidence suggests that the various neurobiological actions of DBI and its processing products may be attributable to the ability of these peptides either to bind to BZD recognition sites associated with GABAA receptors or to bind to glial cell MBRs and modulate the rate and quality of neurosteroidogenesis. The neurobiological effects of DBI and its processing products in physiological and pathological conditions (hepatic encephlopaty, depression, panic) concentrations may therefore be explained by interactions with different types of BZD recognition site. In addition, recent reports that DBI and some of its fragments inhibit (in nanomolar concentrations) glucose-induced insulin release from pancreatic islets and bind acyl-coenzyme A with high affinity support the hypothesis that DBI isa precursor of biologically active peptides with multiple actions in the brain and in peripheral tissues.


Life Sci


Life sciences


Costa E,Guidotti A




Has Abstract


1991-01-01 00:00:00














  • Cyclosporine augments reactivity of isolated blood vessels.

    abstract::Administration of cyclosporine (CS) as an immunosuppressive agent in clinical transplantation is associated with multiple side effects including nephrotoxicity and hypertension. These two effects could be related in that the renal changes may be secondary to alterations in organ blood flow. The present studies investi...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Lamb FS,Webb RC

    更新日期:1987-06-29 00:00:00

  • L-dopa is protective against indomethacin-induced small intestinal ulceration in the rat: possible role of an alpha-2-adrenergic mechanism.

    abstract::Dopaminergic agents ameliorate experimentally induced gastroduodenal mucosal injury, but there is no information about their effect on small intestinal mucosa. We studied the effect of L-dopa and related substances on indomethacin-induced intestinal ulceration in the rat. Ulceration was produced by s.c. injection of 3...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kiro A,Zahavi I,Marcus H,Dinari G

    更新日期:1992-01-01 00:00:00

  • Lack of biological activity of preproendothelin [110-130] in several endothelin assays.

    abstract::A 21 amino acid peptide containing the prepropendothelin sequence from amino acids 110 to 130 and two intrachain disulfide bonds was synthesized and tested for biological activity in the following endothelin assays: 1.) a competition binding assay using [125I]ET-1 and dog heart membranes, 2.) three RIA's using 125I-ET...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Cade C,Lumma WC Jr,Mohan R,Rubanyi GM,Parker-Botelho LH

    更新日期:1990-01-01 00:00:00

  • In vitro interaction of premazepam with benzodiazepine receptors in rat brain regions.

    abstract::Premazepam (PRZ) in vitro competitively displaced 3H-diazepam (DIA), 3H-flunitrazepam (FLU) and 3H-RO 15-1788 from their binding sites on rat brain synaptosomes, with a potency intermediate to other benzodiazepines (BDZs), and Hill coefficients near 1 in different brain regions. Incubation at 37 degrees C reduced prem...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Barone D,Colombo G,Glasser A,Luzzani F,Mennini T

    更新日期:1984-07-23 00:00:00

  • Skin permeability of various non-steroidal anti-inflammatory drugs in man.

    abstract::The skin permeabilities of a series of eight salicylates and ten non-steroidal anti-inflammatory drugs were investigated in human subjects. The logarithms of % absorption through intact skin and of n-octanol/water partition coefficients (log P) of the test compounds were plotted against one another, and a parabolic re...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yano T,Nakagawa A,Tsuji M,Noda K

    更新日期:1986-09-22 00:00:00

  • Cannabinoid type 2 receptor manipulates skeletal muscle regeneration partly by regulating macrophage M1/M2 polarization in IR injury in mice.

    abstract:AIMS:The beneficial effects of cannabinoid type 2 receptor (CB2R) activation have been verified in various tissue repair processes. Our recent study revealed CB2R activation promotes myogenesis partly through Nrf2 signaling in a mouse skeletal muscle ischemia-reperfusion (IR) injury model. Other relevant mechanisms nee...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Jiang P,Wang L,Zhang M,Zhang M,Wang C,Zhao R,Guan D

    更新日期:2020-09-01 00:00:00

  • Verapamil prevents isoproterenol-induced cardiac failure in the rat.

    abstract::Virgin, male Sprague-Dawley rats were used to study the production of cardiac failure by a single subcutaneous injection of 85 mg/kg dl-isoproterenol (ISO) and the possible preservation of cardiac function by a pre-treatment of 50 mg/kg verapamil (VER) 5 min prior to 85 mg/kg ISO. At 24 hrs after drug injections cardi...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yeager JC,Whitehurst ME

    更新日期:1982-01-18 00:00:00

  • Sulforaphane protects against skeletal muscle dysfunction in spontaneous type 2 diabetic db/db mice.

    abstract:AIMS:Skeletal muscle diseases have become to be the most common complication in patients with type 2 diabetic mellitus (T2DM). However, the effective therapies against skeletal muscle diseases are not yet available. Sulforaphane (SFN) is an organic isothiocyanate found in cruciferous plants. Our aim was to explore whet...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wang M,Pu D,Zhao Y,Chen J,Zhu S,Lu A,Liao Z,Sun Y,Xiao Q

    更新日期:2020-08-15 00:00:00

  • Metformin attenuates hepatoma cell proliferation by decreasing glycolytic flux through the HIF-1α/PFKFB3/PFK1 pathway.

    abstract:AIMS:Enhanced aerobic glycolysis is an essential hallmark of malignant cancer. Blocking the glycolytic pathway has been suggested as a therapeutic strategy to impair the proliferation of tumor cells. Metformin, a widely used anti-diabetes drug, exhibits anti-tumor properties. However, the underlying molecular mechanism...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Hu,Zeng Z,Xia Q,Liu Z,Feng X,Chen J,Huang M,Chen L,Fang Z,Liu Q,Zeng H,Zhou X,Liu J

    更新日期:2019-12-15 00:00:00

  • Pituitary adenylate cyclase activating polypeptide (PACAP) potently enhances tyrosine hydroxylase (TH) expression in adrenal chromaffin cells.

    abstract::In primary cultured bovine adrenal chromaffin cells (BACC), pituitary adenylate cyclase activating polypeptide 1-38 (PACAP) produced a dose related increase in tyrosine hydroxylase (TH) Vmax when measured 48 hours after the beginning of the treatment; a significant increase was observed with 0.5 nM and the maximal ind...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Rius RA,Guidotti A,Costa E

    更新日期:1994-01-01 00:00:00

  • Stimulation of mitochondrial pyruvate transport in rat renal cortex by phenylephrine.

    abstract::Phenylephrine effect on liver and kidney cortex mitochondrial pyruvate concentration was investigated. While in liver the alpha 1-adrenergic agent produced a decrease in pyruvate content, a significant increase was observed in kidney, even in the presence of 0.5 mM alpha-cyano-4-hydroxy-cinnamate. These changes were n...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Oliver J,Sola MM,Salto R,Vargas AM

    更新日期:1990-01-01 00:00:00

  • Strain specific differences in memory and neuropathology in a mouse model of Alzheimer's disease.

    abstract:AIMS:Studies using transgenic mouse strains that incorporate Alzheimer's disease (AD) mutations are valuable for the identification of signaling pathways, potential drug targets, and possible mechanisms of disease that will aid in our understanding of AD. However, reports on the effects of specific AD mutations (Swedis...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Glazner KA,Odero GL,Anema E,Motnenko A,Schapansky J,Grossman D,Oliver DR,Glazner GW,Albensi BC

    更新日期:2010-06-19 00:00:00

  • Characterization of Wistar-Kyoto rats showing hyperadiponectinemia.

    abstract:AIMS:Wistar-Kyoto rats (HA-WKY) kept in the author's laboratory showed higher levels of serum adiponectin (approximately 4-fold) compared with Wistar-Kyoto/Izm rats (WKY/Izm), a WKY standard strain, at 6weeks old. In a preliminary study, HA-WKY but not WKY/Izm showed hyperinsulinemia and severe hypercholesterolemia whe...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Inoue T,Takemori K,Yamamoto K,Ito H

    更新日期:2010-02-27 00:00:00

  • Protease inhibitors protect macrophages from lipopolysaccharide-induced cytotoxicity: possible role for NF-kappaB.

    abstract::Recent studies suggest lipopolysaccharide (LPS) mediated cell death as underlying mechanism of hyporesponsiveness and dysfunction of macrophages in the late phase of septic shock. In the present study LPS (0.001 - 30 microg/ml) caused a concentration-dependent toxicity in the macrophage cell line (J774.1A) within 24 h...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Abate A,Schröder H

    更新日期:1998-01-01 00:00:00

  • Structural derivatives of pindolol: relationship between in vivo and in vitro potencies for their interaction with central beta-adrenergic receptors.

    abstract::Although (-)-125I-iodopindolol (IPIN) can be used to label beta-adrenergic receptors in the central nervous system (CNS) in vivo, use of this ligand for receptor imaging studies in humans may be limited due to its relatively poor penetration into the CNS. A series of derivatives related to pindolol was therefore studi...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Tejani-Butt SM,Brunswick DJ

    更新日期:1987-08-24 00:00:00

  • Importance of iron location in iron-induced hydroxyl radical production by brain slices.

    abstract::Iron imbalance has been implicated in oxidative injury associated with many brain diseases. The present study investigated the importance of iron location in hydroxyl radical (.OH) generation and the link between .OH production evaluated by the salicylate method and lipid peroxidation monitored by thiobarbituric acid-...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Demougeot C,Marie C,Beley A

    更新日期:2000-06-16 00:00:00

  • Time course for labeling of brain membrane phosphoinositides and other phospholipids after intracerebral injection of [32P]-ATP. Evaluation by an improved HPTLC procedure.

    abstract::An improved two-dimensional HPTLC procedure was developed for separating phospholipids including individual phosphoinositides, phosphatidic acids and plasmalogens. This procedure was used to examine the time course for uptake of label by phospholipids in brain subcellular membranes after intracerebral injection of [ga...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sun GY,Lin TN

    更新日期:1989-01-01 00:00:00

  • Role of nitric oxide in lower esophageal sphincter relaxation to swallowing.

    abstract::Studies were performed in the opossum to define the role of the L-arginine-nitric oxide (NO) pathway in lower esophageal sphincter (LES) relaxation to swallowing and vagal stimulation in viv and intramural nerve stimulation in vitro. In vivo, L-NAME, a water soluble NO synthase (NOS) inhibitor, caused antagonism of LE...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yamato S,Saha JK,Goyal RK

    更新日期:1992-01-01 00:00:00

  • Regulation of fatty acid synthesis and Delta9-desaturation in senescence of human fibroblasts.

    abstract:AIMS:Normal human cells in culture progressively lose their capacity for replication, ending in an irreversible arrested state known as replicative senescence. Senescence has been functionally associated to the process of organismal ageing and is also considered a major tumor-suppressing mechanism. Although a great dea...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Maeda M,Scaglia N,Igal RA

    更新日期:2009-01-16 00:00:00

  • In vivo measurements of the cerebral perfusion and cardiovascular effects of the novel guanidine ME10092 in the non-human primate, Papio ursinus.

    abstract::The novel guanidines N-(3,4-dimethoxy-2-chlorobenzylideneamino)-guanidine (ME 10092) and N-(3,4-dimethoxy-2-chlorobenzylideneamino)-N1-hydroxyguanidine (PR5) were recently reported to exhibit promising cardioprotective activities in myocardial ischaemia and reperfusion in rats. The current study investigated for the f...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Oliver DW,Dormehl IC,Wikberg JE,Louw WK,Dambrova M,van Gelder A,Kilian E,van Rensburg EJ

    更新日期:2004-09-10 00:00:00

  • Effects of the cannabinoid receptor agonist CP 55,940 and the cannabinoid receptor antagonist SR 141716 on intracranial self-stimulation in Lewis rats.

    abstract::Lewis rats were trained to self-stimulate the medial forebrain bundle (MFB) using a rate-frequency paradigm. They were then tested for the effects of the cannabinoid receptor agonist CP 55,940, the selective cannabinoid receptor antagonist SR 141716 and the dopamine D1 receptor antagonist SCH 23390. CP 55,940 (0, 10, ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Arnold JC,Hunt GE,McGregor IS

    更新日期:2001-11-21 00:00:00

  • Ginsenoside Rg1-induced activation of astrocytes promotes functional recovery via the PI3K/Akt signaling pathway following spinal cord injury.

    abstract:AIMS:To determine whether ginsenoside Rg1 is involved in scratch wound healing through altered expression of related molecules in astrocytes and improved functional recovery after spinal cord injury (SCI). MATERIALS AND METHODS:Astrocytes were isolated from rats, followed by Rg1 treatment. The wound healing test was p...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xu L,Tang YY,Ben XL,Cheng MH,Guo WX,Liu Y,Lu ZF,Deng JL

    更新日期:2020-07-01 00:00:00

  • Rapamycin and MCC950 modified gut microbiota in experimental autoimmune encephalomyelitis mouse by brain gut axis.

    abstract:AIMS:Multiple sclerosis (MS) whose pathogenesis is still unclear is a chronic progressive disease in the central nervous system. Gut microbiota can directly or indirectly affect the immune system through the brain gut axis to engage in the occurrence and development of the disease. MATERIALS AND METHODS:C57BL/6 mice w...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xu L,Zhang C,He D,Jiang N,Bai Y,Xin Y

    更新日期:2020-07-15 00:00:00

  • Differential effects of bromopride and domperidone on cholinesterase activity in rat tissues.

    abstract::Bromopride (BRO) and domperidone (DOMP) are dopamine D2 blocking agents used in gastroenterology clinics because of their anti emetic effect as well as their central and peripheral actions of increasing gastrointestinal motor activity. The rationale for these experiments was to compare BRO- and DOMP-effects on plasma,...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nasello AG,Gidali D,de Sá-Rocha LC,Felicio LF

    更新日期:1995-01-01 00:00:00

  • Bone fracture healing is delayed in splenectomic rats.

    abstract:AIMS:Splenectomy is sometimes required in bone fracture patients. The present study aims to investigate the effect of splenectomy on fracture healing in rats. MAIN METHODS:Rats underwent osteotomy were subjected to splenectomy. The effects of splenectomy were evaluated at day 0, 3, 15 and 30 post-fracture. Double immu...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xiao W,Hu Z,Li T,Li J

    更新日期:2017-03-15 00:00:00

  • Effect of stress on amino acids and related compounds in various tissues of the rat.

    abstract::The composition of various amino acids and related compounds in the aorta, ventricle, atria, liver, kidney, pancreas, bronchi and adrenals of rats is presented. These patterns are qualitatively similar, but quantitatively different. Stress changed these patterns. In the aorta, alpha-aminobutyric acid and ammonia are d...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Decker R,von Stuckrad-Barre S,Milakofsky L,Hofford JM,Harris N,Vogel WH

    更新日期:1995-01-01 00:00:00

  • Proposing interactions between maternal phospholipids and the one carbon cycle: A novel mechanism influencing the risk for cardiovascular diseases in the offspring in later life.

    abstract::Studies have adequately demonstrated the importance of maternal nutrition, particularly, micronutrients (folic acid, vitamin B12) and long chain polyunsaturated fatty acids (LCPUFAs) in determining pregnancy outcome. Reports indicate that children born preterm or to mothers with preeclampsia are at increased risk of d...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Khot V,Chavan-Gautam P,Joshi S

    更新日期:2015-05-15 00:00:00

  • Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.

    abstract:AIMS:Honokiol is a natural product extracted from herbal plants such as the Magnolia species which have been shown to exhibit anti-tumor and anti-metastatic properties. However, the effects of honokiol on thyroid cancers are largely unknown. MATERIALS AND METHODS:To determine whether honokiol might be useful for the t...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chou HC,Lu CH,Su YC,Lin LH,Yu HI,Chuang HH,Tsai YT,Liao EC,Wei YS,Yang YT,Chien YA,Yu XR,Lee YR,Chan HL

    更新日期:2018-08-15 00:00:00

  • A new hypoglycemic mechanism of catalpol revealed by enhancing MyoD/MyoG-mediated myogenesis.

    abstract:AIMS:Enhancing myogenesis has been identified as a possible target to improve insulin sensitivity and protect against metabolic diseases. Catalpol, an iridoid glycoside, has been shown to exert a hypoglycaemic effect by improvement of insulin sensitivity; however, the underlying mechanism remains unknown. In this study...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xu D,Wang L,Jiang Z,Zhao G,Hassan HM,Sun L,Fan S,Zhou Z,Zhang L,Wang T

    更新日期:2018-09-15 00:00:00

  • A new NPY-antagonist strongly stimulates apoptosis and lipolysis on white adipocytes in an obesity model.

    abstract::Neuropeptide Y (NPY) is a 36 amino acid peptide released in central and peripheral mammalian neurons, which appears to contribute to adiposity regulation by increasing food intake, thus promoting weight gain on animals. Nevertheless, little is known about NPY direct actions on white adipocytes. This trial, which was d...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Margareto J,Aguado M,Osés-Prieto JA,Rivero I,Monge A,Aldana I,Marti A,Martínez JA

    更新日期:2000-11-24 00:00:00