Abstract:
AIM:The action of oestrogen has traditionally been attributed to the activation of nuclear receptors (ERα and ERβ). A third receptor, the G protein-coupled oestrogen receptor (GPER), has been described as mediator of the rapid action of oestrogen. Based on the possible protective role of oestrogen in the cardiovascular system, the present study was designed to determine whether selective GPER activation induces relaxation of mesenteric resistance arteries in both sexes and which signalling pathways are involved. MAIN METHODS:Third-order mesenteric arteries were isolated, and concentration-response curves were plotted following the cumulative addition of the selective GPER agonist G-1 (1nM-10μM) following induction of contraction with phenylephrine (3μM). The vasodilatory effects of G-1 were assessed before and after removal of the endothelium or incubation for 30min with nitric oxide synthase (Nω-nitro-L-arginine methyl ester - L-NAME, 300μM) and cyclooxygenase (indomethacin - INDO, 10μM) inhibitors alone or combined, PI3K-Akt pathway inhibitor (LY-294,002, 2.5μM) or a potassium channel blocker (tetraethylammonium - TEA, 5mM). GPER immunolocalisation was also performed on the investigated arteries. KEY FINDINGS:The tested GPER agonist induced concentration-dependent relaxation of the mesenteric resistance arteries without differences related to sex that were partially endothelium dependent, mainly mediated by the PI3K-Akt-eNOS pathway and attenuated by nonspecific potassium channel blockade. In addition, the endothelial GPER immunolocalisation was stronger among females. SIGNIFICANCE:This evidence provides a new perspective for understanding the mechanisms involved in the vascular responses triggered by oestrogen via GPER in both sexes.
journal_name
Life Scijournal_title
Life sciencesauthors
Peixoto P,Aires RD,Lemos VS,Bissoli NS,Santos RLDdoi
10.1016/j.lfs.2017.06.020subject
Has Abstractpub_date
2017-08-15 00:00:00pages
21-27eissn
0024-3205issn
1879-0631pii
S0024-3205(17)30306-5journal_volume
183pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::The presence of gonadotropin-releasing hormone (GnRH) binding sites in biopsy samples of human epithelial ovarian cancer and ovarian tumor cell lines as well as the demonstration of the inhibitory effects of GnRH analogues on the growth of these cells raised the possibility that GnRH is produced locally by ovarian can...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00173-8
更新日期:1998-01-01 00:00:00
abstract::The effects of agents that modulate intracellular release of calcium and protein kinase C (PKC) activation on noradrenaline (NA)-induced contractions of epididymal vas deferens in calcium-free/EGTA (1 mM) medium were investigated. NA (100 microM) or methoxamine (100 microM) evoked repeatable contractions. Clonidine (1...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00231-3
更新日期:1999-01-01 00:00:00
abstract::The effects of adenosine on the amylase secretion from rat pancreatic lobules have been studied. Adenosine induces a dose-dependent stimulation on amylase release, which is maximal at a concentration of 10(-4) M. It has been observed a clear inhibition of this secretory action when atropine was added whereas no amylas...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02140-e
更新日期:1995-01-01 00:00:00
abstract:AIM:Although constitutive autophagy is linked to redox state and participates in cell homeostasis, it is scarcely known if redox state, autophagy, and lysosomal function depend on sex, a factor that largely influences health and diseases. Therefore, we evaluated the existence of sex differences in redox state and const...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.06.001
更新日期:2013-08-06 00:00:00
abstract::The effects of three local anesthetics, lidocaine, dibucaine, and tetracaine, on Na+/H+ antiporter activity were examined in brush border membrane-reconstituted vesicles. Lidocaine at 10 microM inhibited H+ efflux in the presence of an inward Na+ gradient, suggesting that this anesthetic specifically inhibits the Na+/...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90172-n
更新日期:1990-01-01 00:00:00
abstract::To assess the effect of pharmacological dose of melatonin on bone metabolism in ovariectomized rats, urinary deoxypyridinoline (a marker of bone resorption) and calcium excretion, circulating levels of calcium, phosphorus and bone alkaline phosphatase activity (a marker of bone formation), and bone mineral density (BM...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01431-x
更新日期:2001-12-21 00:00:00
abstract::The influence of the stage of the estrous cycle on binding of [3H]diazepam was examined in membranes from brains of female mice. In order to conserve endogenous factors such as progesterone, other steroids, or GABA, the assay was performed without the extensive washing procedures typically employed in measurements of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02177-k
更新日期:1995-01-01 00:00:00
abstract::Abnormal activation of the beta-catenin signaling pathway can cause various types of cancer. Activation of Wnt pathway leads to stabilization of the beta-catenin protein, which results in its translocation to the nucleus and the formation of complexes with lymphoid enhancer factor-1 (LEF-1) and other T-cell factor (TC...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.02.009
更新日期:2005-07-29 00:00:00
abstract::Effects of scratching behavior on dermatitis, transepidermal water loss (TEWL) and serum IgE concentrations were examined in NC/Nga (NC) mice with toenails (WIT) and without toenails (WOT). The first study was a preventive treatment done to cut off hind toenails before dermatitis induction and the second study was a t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.07.022
更新日期:2004-12-31 00:00:00
abstract::Experiments were undertaken to examine the characteristics of the adrenergic receptor-coupled cAMP system in rat brain slices. It was found that the potentiation of isoproterenol-stimulated cAMP accumulation by 6-fluoronorepinephrine, an alpha-adrenergic agonist, is largely dependent upon the degree of beta-receptor o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90129-8
更新日期:1985-09-30 00:00:00
abstract:AIMS:Hyperthermia is a characteristic functional effect of sleep deprivation (SD). We hypothesize here that prostaglandin E2 (PGE2) could be involved in hyperthermia induced by sleep deprivation. MAIN METHODS:To address this issue we examined the effects of a selective cyclo-oxygenase-2 inhibitor (COX-2) agent on hype...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.12.009
更新日期:2009-02-27 00:00:00
abstract:AIMS:Various investigations have demonstrated the protective capacity of general anesthetics as neuroprotective agents. The effects of propofol against ischemia are known to reside in its antioxidant properties and its GABAergic activity. Other aromatic alcohols have also been reported as able to protect neurons agains...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.03.005
更新日期:2017-04-15 00:00:00
abstract::Peroxisome-proliferator activated receptor-gamma (PPARgamma) has been demonstrated to exert an inhibitory effect on cell growth in most cell types studied, but its role in colon cancer is still uncertain. The molecular mechanism between the activation of PPARgamma and its consequence is unknown. In the present report,...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01510-2
更新日期:2002-04-19 00:00:00
abstract::Oxidative stress induced cell injury is reported to contribute to the pathogenesis of cerebral ischemia. Reactive oxygen species such as hydrogen peroxide (H2O2) and superoxide radical along with nitric oxide and peroxynitrite generated during ischemia-reperfusion injury, causes the overactivation of poly (ADP-ribose)...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.07.034
更新日期:2006-11-10 00:00:00
abstract::Optically active hydroperoxides of phospholipids including phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidic acid were synthesized, and their cytotoxicity to cultured bovine pulmonary artery endothelial cells was examined. Phospholipid hydroperoxides were readily incorporated into c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00684-9
更新日期:1994-01-01 00:00:00
abstract::Different ligands of both the "peripheral" and the "central" benzodiazepine receptors were tested for their ability to induce human monocyte chemotaxis. Only the ligands interacting with the "peripheral receptor" (diazepam and Ro 5-4864) were active, and their action was blocked by the specific antagonist PK 11-195, b...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90275-8
更新日期:1993-01-01 00:00:00
abstract:AIMS:Metformin is an insulin sensitizing agent with beneficial effects in diabetic patients on glycemic levels and in the cardiovascular system. We examined whether the metabolic changes and the vascular dysfunction in monosodium glutamate-induced obese non-diabetic (MSG) rats might be improved by metformin. MAIN METH...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.11.005
更新日期:2012-01-30 00:00:00
abstract::1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-O-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90129-9
更新日期:1987-04-20 00:00:00
abstract::Short-chain fatty acids (SCFA) are produced by fermentation of water-soluble fiber by anaerobic bacteria in the large bowel. Fiber-rich diets decrease the risk of developing inflammatory bowel disease (IBD) and butyrate enemas are effective as a therapy in some patients. Crohn's disease, one form of IBD, appears to in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00490-9
更新日期:2003-08-15 00:00:00
abstract:AIMS:Endurance exercise causes fatigue due to mitochondrial dysfunction and oxidative stress. In order to find an effective strategy to prevent fatigue or enhance recovery, the effects of a combination of mitochondrial targeting nutrients on physical activity, mitochondrial function and oxidative stress in exercised ra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.11.003
更新日期:2010-01-02 00:00:00
abstract::Changes in AMP-activated protein kinase (AMPK) activity contribute to the regulation of insulin secretion. Troglitazone has been shown to lower serum insulin levels and protect beta cell function. The aim of the present study was to examine the effects of troglitazone on AMPK activity and insulin secretion in beta cel...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.04.034
更新日期:2007-06-20 00:00:00
abstract::Early induction of VEGF was studied in liver, kidney and lung of spontaneously diabetic rats. Western blot analysis, northern hybridization were applied to show the expression of VEGF in different organs. Radiolabelled hypoxia responsive element (HRE) and cAMP responsive element (CRE) oligonucleotides were assayed by ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01327-3
更新日期:2001-10-12 00:00:00
abstract:AIMS:Benzodiazepines (BZDs) produce various pharmacological actions by binding to and allosterically regulating GABAA receptors. Several in vitro studies have demonstrated diazepam, the prototypic BZD, produces a high-dose action that cannot be countered with the classical BZD-binding site antagonist flumazenil. Here, ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117033
更新日期:2019-12-15 00:00:00
abstract::14-Hydroxy-retro-retinol was previously described as an in vivo and in vitro metabolite of retinol. Furthermore, the retinoid 4-hydroxy-retinol was identified as an endogenous occurring retinoid in the amphibian organism and an in vitro metabolite of retinol. We describe in the present study that 14-hydroxy-retro-reti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.08.027
更新日期:2005-02-18 00:00:00
abstract:AIMS:To determine whether increased N-acetyltransferase (NAT) activity might have a toxic effect during development and an influence on folate levels since previous work has shown that only low levels of exogenous NAT can be achieved in constitutionally transgenic mice (Cao et al. 2005). MAIN METHODS:A human NAT1 tet-...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.11.008
更新日期:2010-01-16 00:00:00
abstract:AIMS:The study aimed to investigate whether IL-23 is amplified in monocyte subsets of MP pneumonia and to determine its relevant pathway. MATERIALS AND METHODS:We firstly analyze the IL-23p19 expression in monocyte subgroups in MP pneumonia patients and healthy controls subjects by using flow cytometry. Then, we also ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118149
更新日期:2020-10-01 00:00:00
abstract::To examine the therapeutic effect of 4-(o-benzylphenoxy)-N-methylbutylamine hydrochloride (bifemelane) on neuronal ischemia-reperfusion injury, we evaluated the neurological score in gerbils subjected to unilateral common carotid occlusion. Using the neurological score method, we selected animals which showed stroke s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00297-j
更新日期:1995-01-01 00:00:00
abstract:AIM:This study investigated the effect of voluntary motor effort during a low-intensity (30% maximal voluntary contraction [MVC]) muscle exercise training program on increasing muscle strength. MATERIALS AND METHODS:Eighteen young and healthy individuals were randomly assigned to one of three groups: high mental effor...
journal_title:Life sciences
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.lfs.2017.04.003
更新日期:2017-06-01 00:00:00
abstract:AIMS:Nicotine is a risk factor for various diseases, including osteoporosis, oral cancer, and periodontal disease. Numerous studies have elucidated the effects of nicotine on cell proliferation and differentiation. The purpose of this study was to determine the effects of nicotine on the proliferation and osteoblast di...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.10.019
更新日期:2012-01-16 00:00:00
abstract::The effect of the diterpene forskolin on vascular permeability alone and in combination with bradykinin, prostaglandin E1, adenosine or histamine has been investigated in rats. Vascular permeability in rat skin was measured using [125I]-labelled bovine serum albumin ([125I]BSA) as a tracer. In addition, the effect of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90712-9
更新日期:1983-07-04 00:00:00