Expression of the messenger RNA for gonadotropin-releasing hormone and its receptor in human cancer cell lines.

Abstract:

:The presence of gonadotropin-releasing hormone (GnRH) binding sites in biopsy samples of human epithelial ovarian cancer and ovarian tumor cell lines as well as the demonstration of the inhibitory effects of GnRH analogues on the growth of these cells raised the possibility that GnRH is produced locally by ovarian cancer cells. In order to investigate an autocrine/paracrine regulatory mechanism in human carcinomas, we have studied the expression of GnRH and GnRHR mRNA in human ovarian epithelial cell lines (OVCAR-3 and SKOV-3), human choriocarcinoma cell line (JEG-3) and human hepatocarcinoma cell line (HepG 2). Using primers corresponding to published human GnRH and GnRHR cDNA sequences, predicted PCR products were obtained from these cell lines by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by Southern hybridization. Sequencing analysis of GnRH PCR products showed that their sequences have 100% identity to the published human GnRH cDNA sequence. These results indicated that GnRH and GnRHR genes are expressed in all the cell lines tested in the present study, and strengthen the concept that GnRH may act as an autocrine regulator on the growth of cancer cells.

journal_name

Life Sci

journal_title

Life sciences

authors

Yin H,Cheng KW,Hwa HL,Peng C,Auersperg N,Leung PC

doi

10.1016/s0024-3205(98)00173-8

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

2015-23

issue

22

eissn

0024-3205

issn

1879-0631

pii

S0024320598001738

journal_volume

62

pub_type

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