Expression of the messenger RNA for gonadotropin-releasing hormone and its receptor in human cancer cell lines.


:The presence of gonadotropin-releasing hormone (GnRH) binding sites in biopsy samples of human epithelial ovarian cancer and ovarian tumor cell lines as well as the demonstration of the inhibitory effects of GnRH analogues on the growth of these cells raised the possibility that GnRH is produced locally by ovarian cancer cells. In order to investigate an autocrine/paracrine regulatory mechanism in human carcinomas, we have studied the expression of GnRH and GnRHR mRNA in human ovarian epithelial cell lines (OVCAR-3 and SKOV-3), human choriocarcinoma cell line (JEG-3) and human hepatocarcinoma cell line (HepG 2). Using primers corresponding to published human GnRH and GnRHR cDNA sequences, predicted PCR products were obtained from these cell lines by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by Southern hybridization. Sequencing analysis of GnRH PCR products showed that their sequences have 100% identity to the published human GnRH cDNA sequence. These results indicated that GnRH and GnRHR genes are expressed in all the cell lines tested in the present study, and strengthen the concept that GnRH may act as an autocrine regulator on the growth of cancer cells.


Life Sci


Life sciences


Yin H,Cheng KW,Hwa HL,Peng C,Auersperg N,Leung PC




Has Abstract


1998-01-01 00:00:00














  • Fluvastatin attenuates IGF-1-induced ERK1/2 activation and cell proliferation by mevalonic acid depletion in human mesangial cells.

    abstract:AIMS:Insulin-like growth factor (IGF)-1 is a major mitogenic growth factor for mesangial cells (MCs). Statins slow the progression of chronic kidney disease by affecting inflammatory cell signaling pathways, in addition to improving lipid profile, however, no studies have investigated the effects of fluvastatin on mito...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Shibata T,Tamura M,Kabashima N,Serino R,Tokunaga M,Matsumoto M,Miyamoto T,Miyazaki M,Furuno Y,Takeuchi M,Abe H,Okazaki M,Otsuji Y

    更新日期:2009-05-22 00:00:00

  • Functional and metabolic evidence for two different vanilloid (VN1 and VN2) receptors in perfused rat hindlimb.

    abstract::Vanilloid spice principles, including capsaicin, stimulate vasoconstriction in the rat hindlimb perfused at constant flow and, depending on dose, either stimulate or inhibit oxygen consumption by this vascular bed. We now present metabolic and functional evidence for two different vanilloid (VN1 and VN2) receptor type...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Colquhoun EQ,Eldershaw TP,Bennett KL,Hall JL,Dora KA,Clark MG

    更新日期:1995-01-01 00:00:00

  • Glycosylated hemoglobin and fructosamines: does their determination really reflect the glycemic control in diabetic patients?

    abstract::The present experiment was designed to determine whether scavenging capacity of serum, in addition to glucose level, influences hemoglobin and serum protein glycosylation in non-insulin dependent diabetic patients. For this purpose forty-seven patients homogeneous for age, disease duration, therapy and glyco-metabolic...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Testa R,Testa I,Manfrini S,Bonfigli AR,Piantanelli L,Marra M,Pieri C

    更新日期:1996-01-01 00:00:00

  • Effect of aging on recovery of striatal dopamine receptors following N-ethoxycarbonyl-2-ethoxy-1, 2-dihydroquinoline (EEDQ) blockade.

    abstract::The recovery rate of striatal dopamine receptors following blockade by N-ethoxycarbonyl-2-ethoxy-1, 2-dihydroquinoline (EEDQ) in vivo is reduced by 25-35% in striata from senescent Wistar rats when compared to mature counterparts. No differences in binding affinity for [3H]-spiperone were observed for the different ag...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Henry JM,Roth GS

    更新日期:1984-08-20 00:00:00

  • Calcium-dependence of serotonin-mediated aldosterone secretion and differential effects of calcium antagonists.

    abstract::The requirement for calcium in the serotonin-mediated aldosterone secretion was investigated using rat adrenal capsular cells. In the calcium-free medium both basal as well as serotonin-stimulated aldosterone secretion (at concentrations of 10(-7) M and 10(-8) M of serotonin) were significantly impaired. The effects o...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Ganguly A,Hampton T

    更新日期:1985-04-15 00:00:00

  • Development of muscarinic receptors and regulation of secretory responsiveness in rodent sweat glands.

    abstract::Sweat glands are innervated by sympathetic neurons which undergo a change in transmitter phenotype from noradrenergic to cholinergic during development. As soon as the glands begin to differentiate, M3 muscarinic receptor mRNA and binding sites are detectable. Receptor expression appears in the absence of innervation ...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Landis SC

    更新日期:1999-01-01 00:00:00

  • Acute paternal alcohol exposure impairs fertility and fetal outcome.

    abstract::An acute injection of an intoxicating dose of alcohol to male rats 24 hours prior to breeding with drug-naive females produced no discernible effect on copulatory activity, as reflected in vaginal plugs, but resulted in markedly (> 50%) reduced pregnancy rates. Fetal outcome was also markedly affected in offspring sir...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Cicero TJ,Nock B,O'Connor LH,Sewing BN,Adams ML,Meyer ER

    更新日期:1994-01-01 00:00:00

  • Thalidomide derivatives and the immune system. 6. Effects of two derivatives with no obvious teratogenic potency on the pattern of integrins and other surface receptors on blood cells of marmosets.

    abstract::The two thalidomide (Thd) derivatives beta-EM12 and phthalimidophthalimide (Phtpht), which exhibit no obvious teratogenicity, were tested for their ability to induce changes in the pattern of lymphocyte subpopulations, and especially changes in integrin receptors, in marmosets (Callithrix jacchus). Previously, Thd and...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nogueira AC,Neubert R,Felies A,Jacob-Müller U,Frankus E,Neubert D

    更新日期:1996-01-01 00:00:00

  • Morphine treatment in vitro or in vivo decreases phagocytic functions of murine macrophages.

    abstract::Studies were performed to compare in vitro and in vivo effects of morphine on the phagocytic function of murine peritoneal macrophages. Macrophage monolayers were incubated with Candida albicans for 30 min in the absence of autologous serum. Morphine added in vitro was found to decrease both the phagocytic activity (p...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Rojavin M,Szabo I,Bussiere JL,Rogers TJ,Adler MW,Eisenstein TK

    更新日期:1993-01-01 00:00:00

  • Melatonin effect on serotonin uptake and release in rat platelets: diurnal variation in responsiveness.

    abstract:UNLABELLED:The present study was conducted to examine whether melatonin impairs serotonin (5HT) release and uptake in rat platelets. Exposure of platelet-rich plasma samples (PRP) to melatonin induced a concentration-dependent inhibition of 5HT uptake and the value of IC50 was 1.3 x 10(-3) M. We have also investigated ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Martín FJ,Atienza G,Aldegunde M,Míguez JM

    更新日期:1993-01-01 00:00:00

  • Effects of alprazolam and clonidine on carbon dioxide-induced increases in anxiety ratings in healthy human subjects.

    abstract::In order to investigate possible neurobiologic mechanisms underlying carbon dioxide-induced anxiety, the effects of oral alprazolam 0.75 mg and intravenous clonidine 2 mcg/kg on CO2-induced increases in ratings of subjective anxiety, pulse rate, and ventilation were measured in healthy human subjects. Pretreatment wit...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Woods SW,Krystal JH,Heninger GR,Charney DS

    更新日期:1989-01-01 00:00:00

  • Adenosine uptake sites in dog heart and brain; interaction with calcium antagonists.

    abstract::[3H] Nitrobenzylthioinosine (NBI) binding is characterized in dog heart and brain. Evidence is presented suggesting that [3H]NBI is binding to the adenosine uptake site in both tissues. Physiologic studies in open-chested dogs clearly demonstrate that NBI acts as a coronary vasodilator, consistent with an action at th...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Marangos PJ,Finkel MS,Verma A,Maturi MF,Patel J,Patterson RE

    更新日期:1984-09-03 00:00:00

  • Effects of Chan Su, a traditional Chinese medicine, on the calcium transients of isolated cardiomyocytes: cardiotoxicity due to more than Na, K-ATPase blocking.

    abstract::Extracts of Chan Su, a traditional Chinese medication used as a topical anesthetic and cardiac medication, were incubated with cardiomyocytes that had been loaded with a calcium specific fluorescent probe. Calcium transients were measured by real-time fluorescence spectrophotometry following treatment. The transients ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bick RJ,Poindexter BJ,Sweney RR,Dasgupta A

    更新日期:2002-12-27 00:00:00

  • Effects of high sodium intake diet on the vascular reactivity to phenylephrine on rat isolated caudal and renal vascular beds: Endothelial modulation.

    abstract::High salt intake is involved in the genesis of hypertension and vascular changes in salt-sensitive patients. Although many mechanisms have been proposed, the underlying mechanisms of these alterations in healthy rats are not completely elucidated. The aim of this study was to investigate if male Wistar rats fed a high...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: dos Santos L,Gonçalves MV,Vassallo DV,Oliveira EM,Rossoni LV

    更新日期:2006-04-04 00:00:00

  • Age-associated changes and sex differences in urinary 6-sulphatoxymelatonin circadian rhythm in the rat.

    abstract::The circadian rhythm of 6-sulphatoxymelatonin (aMT6s) excretion has been determined in male and female rats at 3 weeks and at 2, 8, 14 and 20 months of age. All animals have a pronounced circadian pattern of aMT6s excretion under a 12 hour dark: 12 hour light cycle. A significant increase in aMT6s excretion is observe...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yie SM,Liu GY,Johansson E,Brown C,Brown GM

    更新日期:1992-01-01 00:00:00

  • Recent advances and new insights into muscular lymphangiogenesis in health and disease.

    abstract::The lymphatic vessels have been implicated in maintenance of interstitial fluid homeostasis and immune responses, and pathological conditions including inflammation, wound healing, lymphedema and tumor progression. The knowledge about the lymphatic structure and function in muscles, especially in muscular disorders, r...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Ji RC

    更新日期:2018-10-15 00:00:00

  • Prolonged beta-adrenoceptor stimulation increases the amount of GDP-binding protein in brown adipose tissue mitochondria.

    abstract::Cold acclimation increases the thermogenic capacity of brown adipose tissue (BAT) and this is associated with both a general hypertrophy of the tissue and a selective increase in the proportion of a specific 32,000 molecular weight, GDP-binding protein in the mitochondrial membrane. This protein regulates the activity...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Young P,Wilson S,Arch JR

    更新日期:1984-03-19 00:00:00

  • The blood pressure effects of alpha-adrenoceptor antagonists injected in the medullary site of action of clonidine: the nucleus reticularis lateralis.

    abstract::We administered a series of alpha-blocking drugs to the nucleus reticularis lateralis (NRL) of the medulla oblongata, the main site for the hypotensive action of clonidine. These experiments were performed on pentobarbital anaesthetized cats. Drugs were injected through a needle which was stereotaxically inserted. Pra...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bousquet P,Feldman J

    更新日期:1987-03-16 00:00:00

  • Mechanisms and treatments of myocardial injury in patients with corona virus disease 2019.

    abstract::The infection epidemic event of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was formally declared a pandemic by World Health Organization on March 11th, 2020. Corona Virus Disease 2019 (COVID-19) is caused by SARS-CoV-2, a new type of coronavirus, which has high contagion and mainly causes respira...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Peng W,Wu H,Tan Y,Li M,Yang D,Li S

    更新日期:2020-12-01 00:00:00

  • Alpha-adrenoceptor-mediated glucose release from perifused catfish hepatocytes.

    abstract::In fish liver catecholamines bind to beta-adrenoceptors (AR) and increase glucose release via cAMP augmentation. Alpha1-AR have recently been shown to mediate IP3 and Ca2+ elevation in catfish and eel hepatocytes, although their coupling to a physiological response has remained doubtful. We have perifused isolated cat...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Fabbri E,Buzzi M,Biondi C,Capuzzo A

    更新日期:1999-01-01 00:00:00

  • Sleep deprivation reduces the recovery of muscle injury induced by high-intensity exercise in a mouse model.

    abstract::Sleep is crucial to improve athlete performance and their circadian rhythm, but sleep patterns may be disturbed because athletes participate in several competitions. In addition, intensive training programs can cause muscle pain and psychological stress in athletes, resulting in a lack of sleep. Sleep also plays a cri...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yang DF,Shen YL,Wu C,Huang YS,Lee PY,Er NX,Huang WC,Tung YT

    更新日期:2019-10-15 00:00:00

  • Insulin resistance does not impair contractile responses of cerebral arteries.

    abstract::Insulin resistance (IR) impairs endothelium-mediated vasodilation in cerebral arteries as well as K+ channel function in vascular smooth muscle. Peripheral arteries also show an impaired endothelium-dependent vasodilation in IR and concomitantly show an enhanced contractile response to endothelin-1 (ET-1). However, th...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Simandle SA,Erdös B,Snipes JA,Miller AW,Busija DW

    更新日期:2005-09-16 00:00:00

  • Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds.

    abstract::Grape seed extract (GSE) is a commonly available dietary supplement taken for the anti-oxidant activity that's attributed to its proanthocyanidin (oligomers of monomeric polyphenols) content. Similar polyphenol-enriched preparations from blueberries and soy have shown protection against ovariectomy-induced or age-rela...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kim H,Deshane J,Barnes S,Meleth S

    更新日期:2006-03-27 00:00:00

  • Elevation of plasma 7B2 (a novel pituitary protein) in cord blood at obstetrical delivery and the possible correlation with GH.

    abstract::Plasma 7B2-immunoreactivity (7B2-IR) concentrations in umbilical artery (UA), umbilical vein (UV) and maternal vein (MV) were measured by RIA at the time of obstetrical delivery at term. Plasma 7B2-IR concentrations (Mean +/- SEM) in UA (N = 12), UV (N = 16) and MV (N = 16) were 725 +/- 69, 699 +/- 64 and 116 +/- 4.5 ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Iguchi H,Natori S,Nawata H,Kato K,Ibayashi H,Chan JS,Seidah NG,Chretien M

    更新日期:1987-10-19 00:00:00

  • A novel small molecule, NecroX-7, inhibits osteoclast differentiation by suppressing NF-κB activity and c-Fos expression.

    abstract:AIMS:Osteoclasts, the unique bone-resorbing polykaryons, are responsible for many bone-destructive diseases, such as osteoporosis and rheumatoid arthritis. Hence, the regulation of osteoclast formation is considered a potential therapeutic approach for these diseases. In this study, we investigated the effect of a nove...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kim HJ,Yoon KA,Lee MK,Kim SH,Lee IK,Kim SY

    更新日期:2012-11-02 00:00:00

  • LEA-1/ICAM-1 interaction is essentially involved in the pathogenesis of delayed-type hypersensitivity-induced liver injury to picryl chloride.

    abstract::The kinetics of lymphocyte function associated antigen 1 (LFA-1) expression on spleen cells (SPC) and liver non-parenchymal cells (NPC), and intercellular adhesion molecule 1 (ICAM-1) expression on hepatocytes (HC) was examined in acute liver injury mice induced by a DTH reaction to picryl chloride (PCl). The peak exp...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Xu Q,Jiang J,Cao J,Wu F,Fujii H,Saiki I

    更新日期:1998-01-01 00:00:00

  • Aortic coarctation induces oxidative stress in rat tissues.

    abstract::The purpose of this study was to evaluate the induction of oxidative stress in heart and erythrocytes from rats with abdominal aorta coarctation (Coa) compared with sham-operated normotensive controls (Sham). The group of Coa animals developed myocardial hypertrophy, showing heart homogenates markedly increased levels...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Polizio AH,Gorzalczany S,Taira C,Peña C

    更新日期:2006-07-04 00:00:00

  • In vitro comparative analysis of human dental stem cells from a single donor and its neuronal differentiation potential evaluated by electrophysiology.

    abstract:AIMS:The aim of this study was to find out a mesenchymal stem cells (MSCs) source from human dental tissues of the same donor (follicle, papilla and pulp), which exhibits higher neurogenic differentiation potential in vitro. MAIN METHODS:MSCs were isolated from dental tissues (follicle, papilla and pulp) by digestion ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Ullah I,Subbarao RB,Kim EJ,Bharti D,Jang SJ,Park JS,Shivakumar SB,Lee SL,Kang D,Byun JH,Park BW,Rho GJ

    更新日期:2016-06-01 00:00:00

  • The role of the ventromedial hypothalamic area in periprandial glucoregulation.

    abstract::There is a great deal of evidence that the ventromedial hypothalamic area (VMH) plays a significant role in glucoregulation. The present review synthesizes new and existing data in a coherent model of a hypothalamic glucoregulatory control system whose function is to stabilize blood glucose levels in the face of disco...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Storlien LH

    更新日期:1985-02-11 00:00:00

  • Dexamethasone and Tofacitinib suppress NADPH oxidase expression and alleviate very-early-onset ileocolitis in mice deficient in GSH peroxidase 1 and 2.

    abstract::C57BL6/J (B6) mice lacking Se-dependent GSH peroxidase 1 and 2 (GPx1/2-DKO) develop mild to moderate ileocolitis around weaning. These DKO mice have a disease resembling human very-early-onset inflammatory bowel disease (VEOIBD), which is associated with mutations in NADPH oxidase genes. Drugs including dexamethasone ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chu FF,Esworthy RS,Shen B,Gao Q,Doroshow JH

    更新日期:2019-12-15 00:00:00