Abstract：
:Estrogen has diverse effects on the vasculature, such as vasodilation, endothelial growth and inhibition of vascular smooth muscle cell (VSMC) proliferation and migration. However, little is known about the genes that are regulated by estrogen in the vascular wall. Wistar rats were ovariectomized or sham-operated (Sham group), and 2 weeks after the operation, were subjected to subcutaneous implantation of placebo pellets (OVX + V group) or estradiol pellets (OVX + E group). Endothelium-denuded aortic tissue was examined 2 weeks after implantation. By applying high-density oligonucleotide microarray analysis, the expression of approximately 7000 genes was analyzed. Among the genes with different expression levels between the OVX + E group and the OVX + V group, those that have been reported to be expressed in the vasculature or muscle tissue, were chosen. Finally, four genes, caveolin-1, two LIM proteins (enigma and SmLIM) and Id3a, were identified. Microarray as well as real-time polymerase chain reaction showed that the expression levels of these genes were significantly higher in the OVX + E group than in the OVX + V group. To clarify whether estrogen directly upregulates these genes in the vascular wall, Northern blot analysis was performed using cultured rat VSMC. Addition of 100 nmol/L estradiol for 24 hours increased the mRNA levels of all four genes. Although the precise mechanism remains unclear, regulation of these genes by estrogen might contribute to its effect on VSMC.
journal_name
Life Scijournal_title
Life sciencesauthors
Watanabe T,Akishita M,Nakaoka T,He H,Miyahara Y,Yamashita N,Wada Y,Aburatani H,Yoshizumi M,Kozaki K,Ouchi Ydoi
10.1016/j.lfs.2004.03.004subject
Has Abstractpub_date
2004-07-23 00:00:00pages
1219-29issue
10eissn
0024-3205issn
1879-0631pii
S002432050400390Xjournal_volume
75pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Luteolin has been shown to be beneficial to cardiovascular tissues and organs. We aimed to study its vasospasmolytic effects against various vasoconstrictors in the isolated rat coronary arteries (RCAs) and its electrophysiological effects on K+ currents via voltage-gated potassium (Kv) channels and inward rectifi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.02.028
更新日期：2019-03-15 00:00:00
abstract：:Antroduodenal myoelectric activity was recorded in conscious sheep by electrodes chronically implanted in the muscular wall. Furthermore, plasma immunoreactive (i.r.) motilin, somatostatin and bombesin concentrations were determined by RIA. The intravenous infusion of somatostatin (20 ng/kg/min), bombesin (10 ng/kg/mi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00209-3
更新日期：1996-01-01 00:00:00
abstract：:Homocysteine (Hcy), an intermediate in methionine metabolism, has been proposed to be involved in hepatic fibrogenesis. Impaired liver function can alter Hcy metabolism. The aim of the present study was to determine plasma Hcy alterations in acute obstructive cholestasis and the subsequent biliary cirrhosis. Cholestas...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.12.009
更新日期：2005-04-08 00:00:00
abstract：:After 7 years from its cloning, the transient receptor potential vanilloid type-1 (TRPV1) channel remains the sole membrane receptor mediating the pharmacological effects of the hot chilli pepper pungent component, capsaicin, and of the Euphorbia toxin, resiniferatoxin. Yet, this ion channel represents one of the most...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2005.05.021
更新日期：2005-08-19 00:00:00
abstract：:Hypoxia-induced neuronal damage and glutamate release were investigated in a N(2)- or in xenon-atmosphere for embryonic rat cortical neurons; cellular damage and glutamate over-release were observed in N(2)-treated cells whereas xenon protected the cells from the hypoxic insult. The protective effect of xenon was stro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02439-6
更新日期：2003-03-14 00:00:00
abstract：:We reported previously that acetylcholine (ACh)-induced endothelium-dependent relaxation of rat mesenteric microvessels depended both on nitric oxide (NO) and on a charybdotoxin (CTX)-sensitive endothelium-derived hyperpolarizing vasodilator. Cytochrome P450 (CYP)-dependent arachidonic acid metabolites act in some sys...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00413-6
更新日期：2000-02-11 00:00:00
abstract：:It has been shown that the main psychoactive component of marihuana, delta9-tetrahydrocannabinol (THC) has mainly inhibitory effects on the regulation of reproduction. Recently, the purification and availability of the endogenous ligand of the cannabinoid receptor, arachidonyl ethanolamide, anandamide, (ANA) and its s...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(99)00292-1
更新日期：1999-01-01 00:00:00
abstract：:Retinoids have been shown the most powerful inducers of transglutaminase activity, a well known marker of differentiation. In this work, we tested the effects of all-trans retinoic acid and EGF, used alone or in combination, on transglutaminase activity in a squamous, epithelial carcinoma cell line, HEp-2. We demonstr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.04.047
更新日期：2004-10-15 00:00:00
abstract：:Pretreatment with a low dose of 3-nitropropionic acid (3-NPA) has been shown to induce ischemic tolerance in the gerbil hippocampus. It is well known that sublethal (2-min) ischemia also induces ischemic tolerance. To investigate the acquisition of ischemic tolerance with 3-NPA, we examined the protein expression afte...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.01.037
更新日期：2005-10-21 00:00:00
abstract：:Nephrotoxicity is a limiting factor in the use of ifosfamide in children. Despite the co-administration of uroprotective agents such as sodium 2-mercaptoethanesulfonate (mesna), ifosfamide chemotherapy is associated with nephropathy characterized by glomerular toxicity and Fanconi syndrome in many children treated wit...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00915-2
更新日期：2000-11-24 00:00:00
abstract：:The analgesic effects of bestatin and thiorphan, two enzymatic inhibitors protecting endogenous enkephalins from their degradation, and those of DAGO and deltakephalin, respectively mu and delta opioid peptides, are assessed on the electrical stimulation test of the mouse tail. The relative analgesic potency of DAGO a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90595-7
更新日期：1983-01-01 00:00:00
abstract：:The present study investigated the effects of nitric oxide synthase (NOS) inhibitors on the seizure threshold of DMCM in mice. The seizure threshold of DMCM was evaluated using an intravenous infusion technique. The threshold of DMCM was significantly decreased by pretreatment with N-nitro-L-arginine (NOARG; 8 mg/kg) ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00226-9
更新日期：1997-01-01 00:00:00
abstract：AIMS:Endothelin-1 (ET-1) and endothelin-2 (ET-2; Trp(6)Leu(7)ET-1) are expressed by different cell types, but are considered to display identical pharmacological properties on endothelin receptors. We studied agonist-dependent aspects of endothelin(A) (ET(A))-receptor function and the importance of amino acids 6 and 7 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.02.011
更新日期：2012-10-15 00:00:00
abstract：:The present study was planned to determine the mechanisms involved in the renal vasodilation caused by insulin. Changes in flow caused by the intravenous infusion of 0.004 IU/kg/min of insulin at constant heart rate, aortic blood pressure, left ventricular contractility and blood levels of glucose and potassium in the...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01257-7
更新日期：2001-08-24 00:00:00
abstract：:We investigated the effect of social isolation stress on the formation of experimental liver metastasis resulted from intraportal vein (i.p.v.) injection of colon 26-L5 carcinoma cells in male Balb/c mice, and elucidated some of the underlying mechanism involving the effects of this stress on cellular immunity. Increa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00506-3
更新日期：2000-03-31 00:00:00
abstract：:Short-chain fatty acids (SCFA) are produced by fermentation of water-soluble fiber by anaerobic bacteria in the large bowel. Fiber-rich diets decrease the risk of developing inflammatory bowel disease (IBD) and butyrate enemas are effective as a therapy in some patients. Crohn's disease, one form of IBD, appears to in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00490-9
更新日期：2003-08-15 00:00:00
abstract：:We studied the effect of interleukin-1 alpha (IL-1) on corticotropin-releasing hormone (CRH) secretion by explanted rat hypothalami in vitro. We also assessed possible mediation of arachidonic acid metabolites on IL-1-stimulated CRH secretion, by preincubating hypothalami with the cyclooxygenase inhibitor indomethacin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90190-3
更新日期：1990-01-01 00:00:00
abstract：:Cholinergic neurons in the laterodorsal (LDT) and the pedunculopontine (PPT) tegmental nuclei act to promote REM sleep (REMS). The predominantly glutamatergic neurons of the REMS-induction region of the medial pontine reticular formation are in turn activated by cholinergic cells, which results in the occurrence of to...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(99)00649-9
更新日期：2000-04-14 00:00:00
abstract：:CCK-octapeptide (CCK-8) (EC50 = 0.5 nM), in the presence of Li+, increased 3H-inositol phosphate (IP) accumulation in guinea pig gastric glands prelabeled with 3H-inositol. CCK-8 desulfate, human gastrin I and pentagastrin were much less potent than CCK-8. Antagonists of CCK receptors such as proglumide, dibutyryl-c-G...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90392-3
更新日期：1985-03-11 00:00:00
abstract：AIMS:We evaluated if selected pro-inflammatory cytokines and/or the protein suppressor of cytokine signaling 3 (SOCS-3) could account for decreased insulin-stimulated phosphatidylinositol 3-kinase (PI3-K) activity in the skeletal muscle of the obese Zucker rat. MAIN METHODS:Eight lean and eight obese Zucker rats ~4wee...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.08.038
更新日期：2012-10-22 00:00:00
abstract：AIMS:Alterations in the expression of antioxidant enzymes are associated with changes in cancer cell sensitivity to chemotherapeutic drugs (menadione and β-lapachone). Mechanisms of acquisition of resistance to pro-oxidant drugs were investigated using a model of oxidative stress-resistant MCF-7 breast cancer cells (Re...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.12.017
更新日期：2016-01-15 00:00:00
abstract：AIMS:The aims of this study were to evaluate the effects of p62/SQSTM1 expression levels on lipopolysaccharide (LPS)-induced mucus secretion in BEAS-2B bronchial epithelial cells by measuring expression levels of the MUC5AC gene and the Mucin-5AC (MUC5AC) protein. MATERIALS AND METHODS:Bronchial epithelial cells, BEAS...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.09.030
更新日期：2018-10-15 00:00:00
abstract：:The reproductive system effects of cocaine were studied in male rats. The analysis included measurements of circulating levels of luteinizing hormone (LH) and testosterone (T) by radioimmunoassay (RIA). The weights of the testes and sex accessory organs were also assessed and compared with control animals. Dosage leve...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90440-2
更新日期：1989-01-01 00:00:00
abstract：:The conversion of lyso-platelet activating factor (lyso-PAF) to PAF in cultured paranasal sinus mucosa obtained from normal human subjects was studied. The PAF concentration in the medium was determined after addition of lyso-PAF. PAF became detectable at 10 minutes after the addition of 10(-8)M lyso-PAF, and reached ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90483-j
更新日期：1993-01-01 00:00:00
abstract：:The effect of the repeated or continuous administration of an analog of GH releasing factor (GH-RF), D-Tyr-1, D-Ala-2, Nle-27, GH-RF(1-29)-NH2 (DBO-29), on the subsequent response to this peptide was investigated in pentobarbital-anesthetized male rats. A sc administration of this analog induced a greater and more pro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90759-4
更新日期：1987-06-22 00:00:00
abstract：AIMS:Rg1 is the most active component of traditional Chinese medicine ginseng, having anti-aging and anti-oxidative stress features in multiple organs. Cellular senescence of hepatocytes is involved in the progression of a wide spectrum of chronic liver diseases. In this study, we investigated the potential benefits an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117776
更新日期：2020-08-01 00:00:00
abstract：AIMS:Studies using transgenic mouse strains that incorporate Alzheimer's disease (AD) mutations are valuable for the identification of signaling pathways, potential drug targets, and possible mechanisms of disease that will aid in our understanding of AD. However, reports on the effects of specific AD mutations (Swedis...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.04.014
更新日期：2010-06-19 00:00:00
abstract：:In the present study the formation of thrombin-antithrombin-complexes (TAT) during incubation of thrombin (0.89, 4.5, 8.9 nmol/l) and antithrombin (4.6 micromol/l) on the surface of cultured human EC, derived from different parts of the circulation, and on the surface of human vessel segments was studied. In the absen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00416-0
更新日期：1998-01-01 00:00:00
abstract：:Intracerebroventricularly (i.c.v.)-administered [D-Ala2]deltorphin II (20 micrograms) produced a marked locomotor hyperactivity in male ICR mice. The locomotor hyperactivity induced in response to i.c.v. [D-Ala2]deltorphin II (20 micrograms) was suppressed by pretreatment with naltriben (NTB, 10 micrograms) but not 7-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00307-4
更新日期：1996-01-01 00:00:00
abstract：:The purpose of this study is to report the development of a non-radioactive fluorescent peptide assay for measuring protein kinase C activity (PKC). The assay is based on a glycogen synthase derived fluorescent peptide that is phosphorylated by PKC. Phosphorylation causes the peptide to migrate toward the anode while ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02149-d
更新日期：1995-01-01 00:00:00
