Abstract：
:We reported previously that acetylcholine (ACh)-induced endothelium-dependent relaxation of rat mesenteric microvessels depended both on nitric oxide (NO) and on a charybdotoxin (CTX)-sensitive endothelium-derived hyperpolarizing vasodilator. Cytochrome P450 (CYP)-dependent arachidonic acid metabolites act in some systems as hyperpolarizing vasodilators. We sought to quantitate contributions of such metabolites to the CTX-sensitive component of ACh-induced vasodilation in isolated rat mesenteric resistance arteries. ACh relaxed these vessels nearly completely (93.3+/-1.2%, n = 71); cyclooxygenase inhibition with indomethacin did not diminish this response (94.3+/-11.4%, n = 9). NO synthase inhibition with Nitro-L-arginine (NNLA) reduced relaxation by 30% (n = 54, p<0.05). Pretreatment of vessels with CYP inhibitors, either clotrimazole (CTM) or 17-octadecynoic acid (17-ODYA), or with selective K+ channel inhibitors, either tetraethyammonium acetate (TEA) or CTX, each led to similar small reductions in maximal relaxation (17%, 22%, 16%, and 9% respectively, n = 3-6). Combined pretreatment with NNLA + either (CTM or 17-ODYA) or (TEA or CTX) each led to similar maximal relaxations (52.2+/-4.8%, 50.6+/-9.2, 37.6+/-8.6%, and 44.1+/-11.5%, respectively, n = 6-35; p<0.05 for NNLA+[CTM or TEA or CTX] vs NNLA alone). Combined pretreatment with NNLA+CTM+(CTX or TEA) led to similar maximal relaxations (43.0+/-7.3%, 43.7+/-15%, n = 6-11) that did not differ from values in vessels pretreated with either NNLA+CTM or NNLA+(CTX or TEA). We conclude that the ACh-induced vasodilation was insensitive to cyclooxygenase inhibition, partially sensitive to NO synthase inhibition, and that the K+ channel blockers TEA and CTX identified the same minor component of ACh relaxation as did the CYP inhibitor CTM.
journal_name
Life Scijournal_title
Life sciencesauthors
Davidson-Garcia CA,Nalbantian-Brandt C,Umans JGdoi
10.1016/s0024-3205(00)00413-6subject
Has Abstractpub_date
2000-02-11 00:00:00pages
1097-104issue
12eissn
0024-3205issn
1879-0631pii
S0024320500004136journal_volume
66pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:The N-terminus portion of the POMC leader sequence (signal peptide) was synthesized, and an antiserum was raised against it. A radioimmunoassay was developed which is effective at a dilution of 1:500,000, and sensitive at less than 1 fmole/tube. Since leader sequences often exhibit structural homologies, and since syn...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90135-7
更新日期：1982-11-15 00:00:00
abstract：AIMS:The aims of the study are to investigate whether the combination of adipose-derived stem cells (ADSCs) with decellularized extracellular matrix (dECM) of myocardium can exert synergistic therapeutic effects on acute MI and the underlying mechanism. MAIN METHODS:Myocardial dECM from fresh porcine myocardium was pr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116891
更新日期：2019-12-15 00:00:00
abstract：:Trimidox (3,4,5-trihydroxybenzohydroxamidoxime), a recently synthesized inhibitor of ribonucleotide reductase (RR), was shown to exert anti-proliferative activities in HL-60 and K562 human leukemia cell lines and to prolong the life span of mice inoculated with L1210 mouse leukemia cells. Here we test whether trimidox...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00901-2
更新日期：2000-11-17 00:00:00
abstract：:The potential photoaffinity ligand 14-beta-(o-nitro, p-azido)-cinnamoyl-amino-N-cyclopropylmethylnormorphinone (NAM) and its derivative NOM, lacking the p-azido function, were synthesised and their opiate receptor activity determined in isolated tissue preparations. The ligands showed slow receptor kinetics. NAM was a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90536-2
更新日期：1983-01-01 00:00:00
abstract：:The effects of 14-day physical exercise or iloprost treatment (0.5-2 ng/Kg/min) on endogenous nitric oxide production and neutrophil adhesion were evaluated in 20 patients with peripheral arterial occlusive disease (Fontaine Stage II). Peripheral venous blood samples and 4-h urine samples were collected before, immedi...
journal_title：Life sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0024-3205(99)00550-0
更新日期：1999-01-01 00:00:00
abstract：:Restraint stress is known to induce gastric ulcers in rats. Peripheral sympathetic activity and catecholamines are involved in the pathogenesis of these gastric ulcers. The aim of the present study was to evaluate the effects of restraint on mucosal and muscle catecholamine concentrations in the glandular stomach of r...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90434-s
更新日期：1990-01-01 00:00:00
abstract：:The effects of chronic treatment with antidepressants, verapamil, or lithium on serotonin (5-HT)-induced Ca2+ increase were investigated in single C6BU-1 glioma cells with digital imaging microscopy. Clomipramine and citalopram, at a concentration of 100 nM, decreased the peak values of 5-HT-induced [Ca2+]i changes. V...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00010-6
更新日期：1997-01-01 00:00:00
abstract：:The stability of epidermal growth factor (EGF) in the gastrointestinal humen of 3-day-old suckling and 45-day-old weaned pigs was examined by incubating iodine labeled recombinant human EGF (hEGF) in the gastrointestinal fluids at 37 degrees C and monitoring the generation of acid soluble radioactivity. Chromatographi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00285-8
更新日期：1996-01-01 00:00:00
abstract：:There is increasing evidence that the 5'UTR of mRNAs affects regulation of gene expression in eukaryotic cells. We examined the overexpression of the mu-opioid receptor in High Five insect cells, employing rat mu-receptor cDNA linked to variable lenghts of their native 5'UTR. The sequences employed consist of either 2...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00017-x
更新日期：1999-01-01 00:00:00
abstract：:Calcium entry from extracellular space to acetylcholine (ACh)-sensitive internal stores was investigated in beta-escin permeabilized bovine tracheal smooth muscle. Cyclopiazonic acid (CPA), a selective inhibitor of the sarcoplasmic reticulum (SR) calcium pump, and nifedipine, both inhibited the refilling, and inhibiti...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00152-6
更新日期：1999-01-01 00:00:00
abstract：:The purpose of the this study was to characterize the putative anxiolytic-like effects of the aqueous extract of Albizzia julibrissin stem bark using the elevated plus maze (EPM) in rats. The water extract of Albizzia julibrissin was orally administered at 10, 50, 100 or 200 mg/kg to adult male SD rats, 1 h before beh...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.05.024
更新日期：2004-10-22 00:00:00
abstract：:The endogenous cannabinoid anandamide (AEA) exerts the majority of its effects at CB1 and CB2 receptors and is degraded by fatty acid amide hydrolase (FAAH). FAAH KO mice and animals treated with FAAH inhibitors are impaired in their ability to hydrolyze AEA and other non-cannabinoid lipid signaling molecules, such as...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2012.05.015
更新日期：2013-03-19 00:00:00
abstract：:A simple and sensitive method for the determination of isoprenaline (ISO) in plasma by high performance liquid chromatography (HPLC) with electrochemical detection is presented. Blood pressure and heart rate responses to i.v. infusion of ISO (15, 38 and 76 ng/kg/min) were studied in 15 subjects. Blood samples for ISO ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90044-5
更新日期：1986-08-18 00:00:00
abstract：AIMS:Insulin-like growth factor (IGF)-1 is a major mitogenic growth factor for mesangial cells (MCs). Statins slow the progression of chronic kidney disease by affecting inflammatory cell signaling pathways, in addition to improving lipid profile, however, no studies have investigated the effects of fluvastatin on mito...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.02.022
更新日期：2009-05-22 00:00:00
abstract：:Phospholipase C (PLC), an enzyme involved in the hydrolysis of membrane phospholipid- phosphatidylinositol-bisphosphate to inositol triphosphate and diacylglycerol, and Phorbol 12, myristate 13, acetate (PMA), a tumor promoting agent, could significantly stimulate testosterone (T) secretion from Leydig cells. Arachido...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90223-2
更新日期：1987-07-27 00:00:00
abstract：AIMS:The aim of this study was to find out a mesenchymal stem cells (MSCs) source from human dental tissues of the same donor (follicle, papilla and pulp), which exhibits higher neurogenic differentiation potential in vitro. MAIN METHODS:MSCs were isolated from dental tissues (follicle, papilla and pulp) by digestion ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.04.026
更新日期：2016-06-01 00:00:00
abstract：AIMS:Fenofibrate (FF) is commonly used clinically as a lipid-lowering drug, but whether it participates in endoplasmic reticulum (ER) stress and decreases inflammation in skeletal muscle is still unknown. The aim of this study is to determine whether FF treatment reduces insulin resistance (IR) by alleviating ER stress...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.06.008
更新日期：2016-07-15 00:00:00
abstract：:To investigate the difference of metabolic pharmacokinetics between pure glycyrrhizin (GZ) and GZ in licorice decoction, six New Zealand White rabbits were orally given pure GZ and licorice decoction containing equivalent content of GZ in a randomized crossover design. HPLC methods were used for the quantitation of GZ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.10.020
更新日期：2005-01-21 00:00:00
abstract：:The pure estrogen antagonist ICI-182780, at doses above 50 micrograms/kg, effectively inhibited the initiation of embryo implantation in rats when administered on day 4 of pregnancy (day 1 = sperm positive). The same dose inhibited the implantation initiating effect of intravenous 25 ng of estradiol-17 beta in delayed...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90186-7
更新日期：1993-01-01 00:00:00
abstract：:Recent studies in animals have demonstrated that the steroid, 3 alpha-hydroxy-5 alpha-pregnan-20-one (3A5P), is a potent analgesic when given intracerebroventricularly. Several studies in humans report that spinal steroids are effective in the treatment of chronic low-back pain when given in combination with morphine....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90095-7
更新日期：1992-01-01 00:00:00
abstract：:The rapid progress in the past few years concerning neurotrophic factor research, has greatly stimulated advances in developmental neurobiology of hearing. We have summarized evidence that neurotrophins are expressed by auditory sensory epithelia during the time at which ganglion cells with neurotrophin receptors send...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(94)00506-0
更新日期：1994-01-01 00:00:00
abstract：:Sphinganine (SP) pre-treatment potentiated the retinoic acid (RA)-induced (4-96h exposures) differentiation and increase of alkaline phosphatase (ALP) activity. A higher percentage of SP pre-treated cells in RA exposures resembled mature myelocytes or granulocytes; greater increase in ALP activity was observed. In cel...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90688-y
更新日期：1993-01-01 00:00:00
abstract：:Different receptors mediating the release of endothelium-derived nitric oxide (EDNO) have been identified at endothelial level. In the present study we aimed to characterise, on rabbit aorta by means of pharmacological tools, the generation of EDNO by receptors located on endothelial cell membrane (M3, P2u, P2y) and b...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)01144-2
更新日期：1998-01-01 00:00:00
abstract：:Several recent reports indicate that patients with Huntington's Disease (HD) may manifest membrane abnormalities in a wide variety of cells including peripheral blood lymphocytes. In this study, flow cytometry is used in conjunction with the fluorescent membrane probe, 8-anilino-1-naphthalene sulfonate (ANS), to exami...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90165-1
更新日期：1985-02-18 00:00:00
abstract：:We studied the effect of thapsigargin on intracellular calcium levels ([Ca2+]i) measured by fura-2 fluorimetry in Madin Darby canine kidney (MDCK) cells. Thapsigargin elevated [Ca2+]i dose dependently with an EC50 of approximately 0.15 microM. The Ca2+ signal consisted of a slow rise, a gradual decay and a plateau. De...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00561-x
更新日期：1999-01-01 00:00:00
abstract：:Losartan, a recently developed nonpeptide angiotensin II (Ang II) receptor antagonist, was administered orally to 10-week-old spontaneously hypertensive rats (SHR) for 2 weeks. Cardiac weight and tissue Ang II, as well as plasma renin activity (PRA) and Ang II, were determined. Treatment with Losartan (10 mg/kg per da...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90589-h
更新日期：1992-01-01 00:00:00
abstract：:L-dopa is the major treatment for Parkinson's disease (PD), but its efficacy is limited by the presence of dyskinesia. The dyskinesia develops over a period of exposure to L-dopa and is related to the dosage, therefore, the cause may involve inductive changes that produce toxic levels of metabolites, interfering with ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00543-9
更新日期：2000-04-21 00:00:00
abstract：:The effects of subcutaneous (sc) administration of estradiol (50 micrograms/100 microliters/day x 10 day) or testosterone (15 mg/100 microliters twice a week x 2 weeks) on ANF release were examined in isolated perfused rat atria. The concentration of ANF in the perfusate was measured by radioimmunoassay, under basal c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90245-x
更新日期：1993-01-01 00:00:00
abstract：AIMS AND METHODS:We previously reported that angiotensin II (AngII) restores the vascular reactivity diminished by hemorrhagic shock. In this study, we investigated whether the beneficial effects of AngII are related to regulation of gap junctions (GJs) and connexin43 (Cx43), and the implication of MAPK signaling and m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.11.019
更新日期：2019-01-01 00:00:00
abstract：:Oxidative stress induced cell injury is reported to contribute to the pathogenesis of cerebral ischemia. Reactive oxygen species such as hydrogen peroxide (H2O2) and superoxide radical along with nitric oxide and peroxynitrite generated during ischemia-reperfusion injury, causes the overactivation of poly (ADP-ribose)...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.07.034
更新日期：2006-11-10 00:00:00
