Abstract：
AIMS:The aims of the study are to investigate whether the combination of adipose-derived stem cells (ADSCs) with decellularized extracellular matrix (dECM) of myocardium can exert synergistic therapeutic effects on acute MI and the underlying mechanism. MAIN METHODS:Myocardial dECM from fresh porcine myocardium was prepared in an injectable gel, ADSCs were seeded into the myocardial dECM gels, and then the mixture was injected into the myocardium of the infarct border zone after acute MI, which was induced by ligating the left anterior descending coronary artery in male SD rats, to assess the therapeutic potential. The degree of fibrosis was detected by Masson's trichrome. The evaluation of cardiac function was performed by Electrocardiography. KEY FINDINGS:Myocardial dECM (2.0%) had a suitable aperture and arrangement for cell growth, and also exhibited suitable biomechanical properties. Four-weeks after treatment in vivo, the combination of ADSCs and myocardial dECM could obviously increase angiogenesis, reduce the degree of fibrosis, and decrease infarct size. Furthermore, the combination treatment exerted significant functional improvement. Compared with ADSCs or dECM group alone, the left ventricular ejection fraction (LVEF) in the combination group was 13.4% and 21.8% elevated, respectively. SIGNIFICANCE:The combination of ADSCs and myocardial dECM has synergistic effects on cardiac repair in acute MI.
journal_name
Life Scijournal_title
Life sciencesauthors
Qiao L,Kong Y,Shi Y,Sun A,Ji R,Huang C,Li Y,Yang Xdoi
10.1016/j.lfs.2019.116891subject
Has Abstractpub_date
2019-12-15 00:00:00pages
116891eissn
0024-3205issn
1879-0631pii
S0024-3205(19)30818-5journal_volume
239pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Protein binding determination in post heparin plasma samples is complicated by the continued post heparin lipase activity, in vitro, during the binding analysis. The decomposition of lipoproteins and accumulation of nonesterified fatty acids (NEFA) results in artifically elevated free fractions of many drugs. This art...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90847-0
更新日期：1983-10-10 00:00:00
abstract：:Phospholipase C (PLC), an enzyme involved in the hydrolysis of membrane phospholipid- phosphatidylinositol-bisphosphate to inositol triphosphate and diacylglycerol, and Phorbol 12, myristate 13, acetate (PMA), a tumor promoting agent, could significantly stimulate testosterone (T) secretion from Leydig cells. Arachido...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90223-2
更新日期：1987-07-27 00:00:00
abstract：:Doxorubicin (DOX) induced cardiotoxicity is a life-threatening side effect of chemotherapy and decreased cardiac function can present years after treatment. Despite the investigation of a broad range of pharmacologic interventions, to date the only drug shown to reduce DOX-related cardiotoxicity in preclinical studies...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117070
更新日期：2019-12-15 00:00:00
abstract：:The mechanism by which benzoate enhances total nitrogen excretion was investigated in-situ and in separated rat renal proximal tubules. Orally administered benzoate augmented NH4+, urea and hippurate excretion 2, 1.9 and 76 fold respectively, as compared to baseline for control. Hippurate had similar effects. Benzoate...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90086-q
更新日期：1991-01-01 00:00:00
abstract：AIMS:Fructose administration induces hepatic oxidative stress, insulin resistance, inflammatory and metabolic changes. We tested their potential pathogenic relationship and whether these alterations can be prevented by R/S-α-lipoic acid. MAIN METHODS:Wistar rats received during 21days a commercial diet or the same die...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.07.010
更新日期：2015-09-15 00:00:00
abstract：AIMS:Pulmonary hypertension (PH) leads to right ventricular (RV) adaptation and remodeling and has deleterious long-term effects on RV function. The endocannabinoid receptor CB2 has been associated with protective effects in adaptation and remodeling of the left ventricle after ischemia. Therefore, we investigated the ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.11.003
更新日期：2018-12-15 00:00:00
abstract：AIMS:Amiodarone (AMIO) is currently used in medical practice to reverse ventricular tachycardia. Here we determine the effects of AMIO in the electromechanical properties of isolated left ventricle myocyte (LVM) from mice and guinea pig and in a cellular model of Long QT Syndrome Type 3 (LQTS-3) using anemone neurotoxi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117814
更新日期：2020-08-15 00:00:00
abstract：:Responses of atrial mRNA, atrial peptide and plasma peptide of atrial natriuretic factor (ANF) to treatments to alter fluid volume were studied in rats using RNA dot hybridization assay and radioimmunoassay. Specific changes in the level of ANF mRNA relative to total atrial RNA were observed in atria from sodium restr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90157-2
更新日期：1985-05-13 00:00:00
abstract：:Isolated myocytes for biochemical experiments must be homogeneous and highly enriched in viable cells. For cardiac myocytes, isolation of Ca2+-tolerant cells in high yield and with good viability has been possible from rat. This paper describes myocyte isolation and enrichment procedures which are effective for severa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90319-6
更新日期：1989-01-01 00:00:00
abstract：:In situ radioligand binding and quantitative autoradiography have been used to measure the density of striatal D1-like, D2-like, and GABAA receptors in rats treated with haloperidol at 0.01 or 0.1 mg/kg/ day or chlorpromazine, olanzapine or clozapine at 0.1 or 1.0 mg/kg/day for 1, 3 or 7 months. [3H]SCH23390 binding t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01214-0
更新日期：2001-08-03 00:00:00
abstract：:A series of studies were conducted to determine the effects of leucine-(leu-) enkephalin and methionine-(met-) enkephalin on perfusion pressure. These experiments utilized isolated perfused femoral arterial preparations in pentobarbital-anesthetized cats. The enkephalins were administered intraarterially into the femo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90047-9
更新日期：1982-10-11 00:00:00
abstract：:Hyaluronan (HA) is a multifunctional high molecular weight polysaccharide found throughout the animal kingdom, especially in the extracellular matrix (ECM) of soft connective tissues. HA is thought to participate in many biological processes, and its level is markedly elevated during embryogenesis, cell migration, wou...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2007.02.037
更新日期：2007-05-01 00:00:00
abstract：:Cultured adult skin fibroblasts were studied for binding and functional evidence of muscarinic receptors in order to assess their utility as a model of cholinergic function in affective illness. Saturable, specific, high affinity binding could be demonstrated in intact cells from some cell lines with [3H]-NMS, but not...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90473-x
更新日期：1986-04-14 00:00:00
abstract：AIMS:Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality worldwide. Decrease in NKG2D ligand levels and exhaustion of NK cells in HCC patients are major causes of immune escape, high recurrence, poor prognosis, and low overall survival. Enhancing the susceptibility of HCC to NK cells by upregulating N...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117396
更新日期：2020-04-01 00:00:00
abstract：:The object of this study was to develop an assay for platelet activating factor (PAF) in rat plasma, and to utilise this to determine the effects of dietary fish oil on PAF in normotensive and spontaneously hypertensive rats. Measurement of platelet activating factor in blood plasma has proved difficult because of its...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90143-8
更新日期：1986-05-19 00:00:00
abstract：:Ischemic preconditioning (IP) may protect the lung from ischemia-reperfusion (I/R) injury following cardiopulmonary by-pass and lung or heart transplantation. The present study was undertaken to investigate the role of ATP-dependent potassium channels (K(ATP)) in IP in the isolated buffer-perfused rat lung (IBPR) unde...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.07.011
更新日期：2006-11-02 00:00:00
abstract：:Chronic voluntary running of mice is known to increase the circadian peak of plasma corticosterone without change in the level of adrenocorticotropic hormone (ACTH). In order to investigate how chronic exercise modulates the circadian HPA axis, we used two weeks of voluntary wheel running of mice and confirmed the sig...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.10.023
更新日期：2007-01-30 00:00:00
abstract：UNLABELLED:The present study was conducted to examine whether melatonin impairs serotonin (5HT) release and uptake in rat platelets. Exposure of platelet-rich plasma samples (PRP) to melatonin induced a concentration-dependent inhibition of 5HT uptake and the value of IC50 was 1.3 x 10(-3) M. We have also investigated ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90261-z
更新日期：1993-01-01 00:00:00
abstract：:Estrogen has diverse effects on the vasculature, such as vasodilation, endothelial growth and inhibition of vascular smooth muscle cell (VSMC) proliferation and migration. However, little is known about the genes that are regulated by estrogen in the vascular wall. Wistar rats were ovariectomized or sham-operated (Sha...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.03.004
更新日期：2004-07-23 00:00:00
abstract：:Neuropathic pain syndromes respond poorly to opioid treatment. In our previous studies, we found that intrathecal (i.t.) injection of pertussis toxin (PTX) produces thermal hyperalgesia, which is poorly responsive to morphine and is accompanied by an increase in cerebrospinal fluid (CSF) levels of excitatory amino aci...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.08.020
更新日期：2006-03-13 00:00:00
abstract：:The circadian rhythm of 6-sulphatoxymelatonin (aMT6s) excretion has been determined in male and female rats at 3 weeks and at 2, 8, 14 and 20 months of age. All animals have a pronounced circadian pattern of aMT6s excretion under a 12 hour dark: 12 hour light cycle. A significant increase in aMT6s excretion is observe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90323-h
更新日期：1992-01-01 00:00:00
abstract：:Resveratrol is an antioxidant present in grapes and their related products. We investigated whether dietary resveratrol could inhibit the proliferation and metastasis of tumors and hyperlipidemia in Donryu rats subcutaneously implanted with an ascites hepatoma cell line of AH109A. By feeding 10 or 50 ppm resveratrol i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00469-7
更新日期：2003-08-01 00:00:00
abstract：:The aim of the present work was to study the effects of various durations of fasting (12, 24 and 72 hours) on brown adipose tissue (BAT) thermogenic parameters--cytrochrome-c-oxidase (COX) activity, GDP-binding activity and uncoupling protein (UCP1) content--and also on morphological features of different mitochondria...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00153-2
更新日期：1998-01-01 00:00:00
abstract：:Castanospermine is a potent inhibitor of rat intestinal glycohydrolases in vitro and prevents the hyperglycemic response to an oral sucrose challenge in vivo. Among the glycohydrolases tested, castanospermine was most effective against sucrase with an IC50 of 1.1 x 10(-7) M. In vivo, a significant effect was seen at d...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90546-7
更新日期：1987-11-16 00:00:00
abstract：:Angiotensin (AII) and atrial natriuretic peptide (ANP) exert opposite effects on phosphorylation of a 17.6 kDa nuclear protein from bovine adrenal glomerulosa cells. The protein was separated by sodium dodecyl sulfate electrophoresis and blotted onto polyvinylidene difluoride, and the N-terminal sequence was obtained....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90465-4
更新日期：1990-01-01 00:00:00
abstract：:Though chemokines of the CXC family are thought to play key roles in neoplastic transformation and tumor invasion, information about CXC chemokines in prostate cancer is sparse. To evaluate the involvement of CXC chemokines in prostate cancer, we analyzed the CXC coding mRNA of both chemokine ligands (CXCL) and chemok...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.08.019
更新日期：2006-03-13 00:00:00
abstract：:Immunotherapy methods using potential tumor microenvironment modulators have elicited durable therapeutic responses in cancer treatment. Immune checkpoint molecule programmed cell death-ligand 1 (PD-L1) and oncogenic transcription factor STAT3 (signal transducer and activator of transcription-3) assigned as inhibitory...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118847
更新日期：2021-02-01 00:00:00
abstract：:In recent years, serum S100B has been used as a secondary endpoint in some clinical trials, in which serum S100B has successfully indicated the benefits or harm done by the tested agents. Compared to clinical stroke studies, few experimental stroke studies report using serum S100B as a surrogate marker for estimating ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.06.031
更新日期：2007-08-02 00:00:00
abstract：AIMS:Obesity represents a global health problem. Excessive caloric intake promotes the release of inflammatory mediators by hypertrophic adipocytes and obesity-induced inflammation is now recognized as a risk factor for the development of several diseases, such as cardiovascular diseases, insulin resistance, type-II di...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118618
更新日期：2021-01-01 00:00:00
abstract：:Peroxisome-proliferator activated receptor-gamma (PPARgamma) has been demonstrated to exert an inhibitory effect on cell growth in most cell types studied, but its role in colon cancer is still uncertain. The molecular mechanism between the activation of PPARgamma and its consequence is unknown. In the present report,...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01510-2
更新日期：2002-04-19 00:00:00
