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Isolation and enrichment of Ca2+-tolerant myocytes for biochemical experiments from guinea-pig heart.

Abstract：

:Isolated myocytes for biochemical experiments must be homogeneous and highly enriched in viable cells. For cardiac myocytes, isolation of Ca2+-tolerant cells in high yield and with good viability has been possible from rat. This paper describes myocyte isolation and enrichment procedures which are effective for several species including guinea-pig and rat. New methods for selection of collagenase and viable cells are presented. Using Ca2+-tolerant myocytes obtained from guinea-pig heart and enriched in viable cells, dihydropyridine binding sites are shown to be accessible only in depolarized cells.

journal_name

Life Sci

journal_title

Life sciences

authors

Stemmer P,Akera T,Brody TM,Rardon DP,Watanabe AM

doi

10.1016/0024-3205(89)90319-6

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

1231-7

issue

17

eissn

0024-3205

issn

1879-0631

pii

0024-3205(89)90319-6

journal_volume

44

pub_type

杂志文章

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