Abstract:
:The N-terminus portion of the POMC leader sequence (signal peptide) was synthesized, and an antiserum was raised against it. A radioimmunoassay was developed which is effective at a dilution of 1:500,000, and sensitive at less than 1 fmole/tube. Since leader sequences often exhibit structural homologies, and since synthetic peptides are not readily available, we resorted to an unusual procedure to establish specificity. This involved extraction of pituitary RNA, cell-free translation to produce the pre-prohormones, and purification by B-END and signal antibody affinity columns. The eluates were then tested by SDS gel electrophoresis and by multiple immunoprecipitations. All results showed that the signal antibody captured a single molecular species, approximately 30,000 in MW, which was also captured by the B-END column, and was immunoprecipitable by B-END and ACTH antisera. It therefore appears that this antibody selectively measures the POMC leader sequence and should be valuable in measuring the newly synthesized pre-prohormone.
journal_name
Life Scijournal_title
Life sciencesauthors
Akil H,Shiomi H,Thompson R,Lax S,Coy D,Watson Sdoi
10.1016/0024-3205(82)90135-7subject
Has Abstractpub_date
1982-11-15 00:00:00pages
2271-3issue
20-21eissn
0024-3205issn
1879-0631pii
0024-3205(82)90135-7journal_volume
31pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:Long non-coding RNAs (LncRNAs) play central roles in the formation and development of gastric cancer (GC). The aim of this study was to evaluate the expression of PURPL and NONHSAT062994 and the relationship between their expressions with clinical characteristics in GC. MAIN METHODS:PURPL and NONHSAT062994 LncRNA...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116904
更新日期:2019-11-15 00:00:00
abstract::There is increasing evidence that the 5'UTR of mRNAs affects regulation of gene expression in eukaryotic cells. We examined the overexpression of the mu-opioid receptor in High Five insect cells, employing rat mu-receptor cDNA linked to variable lenghts of their native 5'UTR. The sequences employed consist of either 2...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00017-x
更新日期:1999-01-01 00:00:00
abstract::Castration increases the central nervous system (CNS) sensitivity to hexobarbital as measured by an EEG-threshold method. Testosterone treatment in doses from 1 to 5 mg/kg/day decreases CNS-sensitivity and restores threshold values in castrated rats to a level found in normal young adult male rats. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90385-6
更新日期:1985-03-11 00:00:00
abstract::Extracellular phospholipase A2 (PLA2) with proinflammatory activity has recently been discovered in synovial fluids in inflammatory arthritides. In the search for the sources of synovial fluid PLA2, human synovium and articular cartilage were found to contain large quantities of the enzyme. In rheumatoid arthritis (RA...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90381-k
更新日期:1991-01-01 00:00:00
abstract:AIMS:The type 2 muscarinic receptor (M2R) differs from the other G-protein-coupled muscarinic receptor (type 4, or M4R) in tissue distribution and physiologic effects. We studied the impact of these receptors on sleep and arousal by using M2R and M4R knock-out (KO) mice. MAIN METHODS:M2R and M4R KO and genetically int...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.11.019
更新日期:2010-01-30 00:00:00
abstract::Fibrotic lung diseases qualify among the most dreaded irreversible interstitial pulmonary complications with progressive yet largely unpredictable clinical course. Idiopathic pulmonary fibrosis (IPF) is the most challenging prototype characterized by unknown and complex molecular etiology, severe dearth of non-invasiv...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118201
更新日期:2020-10-01 00:00:00
abstract::Diabetic retinopathy, a common secondary complication of diabetes mellitus, involves extensive damage to the retinal microvasculature. Retina, being a susceptible target, is highly prone to hyperglycemia-induced molecular damages. PPAR receptor, chiefly gamma subtype, mediates numerous responses related to glucose met...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2016.03.054
更新日期:2016-05-15 00:00:00
abstract::Nicotine is the main constituent of tobacco smoke responsible for the elevated risk of the cardiovascular disease and sudden coronary death associated with smoking, presumably by provoking cardiac arrhythmias. The cellular mechanisms may be related to the ability of nicotine to prolong action potentials and to depolar...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00370-7
更新日期:1999-01-01 00:00:00
abstract::Intracerebroventricular (i.c.v.) administration of the neurohypophyseal neuropeptide arginine8-vasopressin (AVP) results in a dose-dependent attenuation of endotoxin-induced fever (EIF) in rats. Specific antagonists of the neuropeptided(CH2)5[Tyr(Me)2]AVP for V1 receptors, d(CH2)5[dlle2lle4]AVP for the V2 receptors an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90448-x
更新日期:1992-01-01 00:00:00
abstract::The novel guanidines N-(3,4-dimethoxy-2-chlorobenzylideneamino)-guanidine (ME 10092) and N-(3,4-dimethoxy-2-chlorobenzylideneamino)-N1-hydroxyguanidine (PR5) were recently reported to exhibit promising cardioprotective activities in myocardial ischaemia and reperfusion in rats. The current study investigated for the f...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.03.030
更新日期:2004-09-10 00:00:00
abstract::By using multiple time-point constant-rate infusions of deuterium-labeled phosphorylcholine, appropriate kinetic parameters were obtained for use in the calculation of the turnover rate of acetylcholine (TRACh) in selected mouse brain regions. After obtaining these data, the relationship between the analgesic agent co...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90499-f
更新日期:1992-01-01 00:00:00
abstract::The concentration of epinephrine, norepinephrine, dopamine, met-enkephalin-, ACTH-, calcitonin- and somatostatin-like immunoreactivity (IR) were determined in the extracts of 9 adrenal pheochromocytomas from 7 patients. Six of these patients had Sipple's syndrome. There was a close correlation between the amounts of m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90652-0
更新日期:1984-07-23 00:00:00
abstract::Hyaluronic acid (HA), a major glycosaminoglycan component of the extracellular matrix, has regulatory influences on cells and cellular activities. To explore the effects of a high concentration (1 mg/mL) of high molecular weight HA (500-730 kD) on U937 macrophage growth dynamics, three factors that influence overall c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.02.038
更新日期:2004-11-12 00:00:00
abstract::The effects of opioids on human subjective olfactory function have rarely been investigated. This is despite the fact that opioid receptors are widely distributed throughout the olfactory systems. Using an established validated test of subjective olfactory function, olfactory threshold, odor discrimination and odor id...
journal_title:Life sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0024-3205(01)01305-4
更新日期:2001-09-28 00:00:00
abstract:AIM:Intra-myocardial injection of adult bone marrow-derived stem cells (MSC) has recently been proposed as a therapy to repair damaged cardiomyocytes after acute myocardial infarction (AMI). PGI(2) has vasodilatation effects; however, the effects of combining both MSC and PGI(2) therapy on AMI have never been evaluated...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.12.020
更新日期:2011-02-28 00:00:00
abstract::The roles of capsaicin-sensitive sensory nerves (CPSN), endogenous nitric oxide (NO), sulfhydryls (SHs), prostaglandins (PGs) in the gastroprotection by momordin Ic, an oleanolic acid oligoglycoside isolated from the fruit of Kochia scoparia (L.) SCHRAD., on ethanol-induced gastric mucosal lesions were investigated in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00241-6
更新日期:1999-01-01 00:00:00
abstract::Hyperglycemia and oxidative stress are the primary stressors that elicit mitochondria specific cell stress in diabetes. Here we hypothesized that elevated level of ROS in high glucose (HG) environment, trigger mitochondrial stress by damaging mitochondrial DNA (mtDNA), altering inflammatory mediators, and neurodegener...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117914
更新日期:2020-09-01 00:00:00
abstract::Central administration of the selective mu opioid agonist, dermorphin, produces a concurrent diuretic and antinatriuretic response in conscious rats. To determine whether central mu opioids differentially affect the renal excretion of water and sodium, we examined changes in renal function produced by intracerebrovent...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00065-e
更新日期:1995-01-01 00:00:00
abstract::Certain things have not changed since my colleague and I last reviewed the role of dietary salt in hypertension [Haddy, F.J., Pamnani, M.B., 1995. Role of dietary salt in hypertension. Journal of the American College of Nutrition 14, 428-438]. Over half of hypertensives are still salt sensitive, i.e., they respond to ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2006.05.017
更新日期:2006-09-20 00:00:00
abstract::The mechanism of spontaneous coronary contractions was investigated in the rabbit. Arteries with intact endothelium did not spontaneously contract. However, spontaneous coronary contractions were generated after the removal of the endothelium. NG-nitro-L-arginine methyl ester and methylene blue, the inhibitors of the ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00492-7
更新日期:1994-01-01 00:00:00
abstract:AIMS:Schisandra is a good choice in Traditional Chinese Medicine for the therapy of cardiovascular diseases, but whether it contains a or some specific component (s) responsible these effects are still unclear. In the present study, we explored whether Schisantherin A (SCA) causes vasorelaxation in isolated rat thoraci...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117357
更新日期:2020-03-15 00:00:00
abstract::The mechanism of fibrate-induced myopathy was investigated in this report. When clofibrate (30 to 300 microM) was applied to L6 rat skeletal myoblasts, dose-dependently apoptosis was observed within 24 h. In the apoptotic myoblasts, a caspase-12 cleavage was observed at 2 h and with following caspases-9 and -3-related...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.08.003
更新日期:2006-03-13 00:00:00
abstract::Injection of carbon tetrachloride (CCl4) intraperitoneally into model animals induces acute liver injury mediated by reactive oxygen species (ROS) as normal metabolites in hepatocytes. In this study, the molecular process in this type of liver injury was analyzed from the aspect of liver function and regulatory factor...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.02.030
更新日期:2004-08-13 00:00:00
abstract::G protein-coupled receptors (GPCRs) are integral membrane proteins that, in response to activation by extracellular stimuli, regulate intracellular second messenger levels via their coupling to heterotrimeric G proteins. GPCR activation also initiates a series of molecular events that leads to G protein-coupled recept...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2003.09.009
更新日期:2003-12-05 00:00:00
abstract::This study examines the effects of prenatal cocaine administration on the development of vascular sympathetic innervation and contractile responsiveness. Rabbits received cocaine (4 mg/kg, iv, bid) or saline during gestational days 8 to 29. Aortas were obtained on postnatal days 10, 20, 30 and 50. Vascular smooth musc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00765-9
更新日期:1994-01-01 00:00:00
abstract::Previous behavioral studies on triazolam (TZ), which are small in number, could only speculate about tolerance to the anxiolytic effect of TZ, as the experiments did not cover sufficient time (of 4 to 7 days) for tolerance to develop. Therefore longer time for chronic TZ administration is used. We investigated the eff...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00612-x
更新日期:2003-10-31 00:00:00
abstract::Foscarnet inhibited noradrenaline and calcium-mediated contractions of the isolated perfused tail artery of the rat. When the noradrenaline contractile response was split into two components, where the first was due to the release of calcium from intracellular stores and the second to the influx of calcium from the ex...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90541-a
更新日期:1993-01-01 00:00:00
abstract:AIMS:Baicalein is a widely used Chinese herbal medicine extracted from Labiatae plants Scutellaria baicalensis Georgi's dry root, which has multiple pharmacological activities. However, the precise mechanism of baicalein against myocardial remodeling remains poorly understood. The aim of our study was to investigate th...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.12.036
更新日期:2016-01-15 00:00:00
abstract::Despite reductions in beta-adrenoreceptor (beta-AR)-mediated inotropic effects induced by sustained sympathetic activation in cardiac disease, whether these changes necessarily result in reductions in systolic function under resting conditions (baseline function) is not clear. Moreover, possible compensatory mechanism...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.047
更新日期:2005-12-12 00:00:00
abstract::A number of potential functions of thioredoxin have been proposed in literature, including a role for DNA replication. The aim of our study was to investigate the effects of thioredoxin from Streptomyces aureofaciens (Trx S.a.) on plasmid DNA. Trx S.a. was incubated with plasmid forms and the incubation product(s) cha...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)01133-8
更新日期:1998-01-01 00:00:00