Abstract：
AIMS:Alterations in the expression of antioxidant enzymes are associated with changes in cancer cell sensitivity to chemotherapeutic drugs (menadione and β-lapachone). Mechanisms of acquisition of resistance to pro-oxidant drugs were investigated using a model of oxidative stress-resistant MCF-7 breast cancer cells (Resox cells). MAIN METHODS:FISH experiments were performed in tumor biopsy and breast cancer cells to characterize the pattern of the NQO1 gene. SNP-arrays were conducted to detect chromosomal imbalances. Finally, the importance of NQO1 overexpression in the putative acquisition of either drug resistance or an increased sensitivity to quinones by cancer cells was investigated by immunoblotting and cytotoxicity assays. KEY FINDINGS:Genomic gain of the chromosomal band 16q22 was detected in Resox cells compared to parental breast cancer MCF-7 cells and normal human mammary epithelial 250MK cells. This genomic gain was associated with amplification of the NQO1 gene in one tumor biopsy as well as in breast cancer cell lines. Using different breast cell models, we found that NQO1 overexpression was a main determinant for a potential chemotherapy resistance or an increased sensitivity to quinone-bearing compounds. SIGNIFICANCE:Because NQO1 is frequently modified in tumors at genomic and transcriptomic levels, the impact of NQO1 modulation on breast cancer cell sensitivity places NQO1 as a potential link between cancer redox alterations and resistance to chemotherapy. Thus, the NQO1 gene copy number and NQO1 activity should be considered when quinone-bearing molecules are being utilized as potential drugs against breast tumors.
journal_name
Life Scijournal_title
Life sciencesauthors
Glorieux C,Sandoval JM,Dejeans N,Ameye G,Poirel HA,Verrax J,Calderon PBdoi
10.1016/j.lfs.2015.12.017subject
Has Abstractpub_date
2016-01-15 00:00:00pages
57-65eissn
0024-3205issn
1879-0631pii
S0024-3205(15)30110-7journal_volume
145pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:The release of liver enzymes is typically used to assess tissue damage following ischemia-reperfusion. The present study was designed to determine the impact of ischemia-reperfusion on liver function and compare these findings with enzyme release. Isolated, perfused rat livers were subjected to low flow ischemia follo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00668-2
更新日期：2000-01-01 00:00:00
abstract：:Guinea pig ileum longitudinal muscle/myenteric plexus preparations contain functional mu- and kappa-opioid receptors. A preparation with blocked kappa receptors was obtained by pretreating guinea pigs with 0.1-10.0 mg/kg intraperitoneal doses of a new kappa-selective affinity label, DIPPA (2-(3,4-dichlorophenyl)-N-met...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00076-3
更新日期：1997-01-01 00:00:00
abstract：:The low Km GTPase displayed an apparent Km value of 0.2-0.4 microM in P2 fractions from whole mouse brain. The activity of this enzyme ranged from 102 (pmols of GTP hydrolysed per micrograms of protein per min) in the striatum to 39 in the pons-medulla oblongata. Intermediate values were found in other structures, 74-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90176-4
更新日期：1993-01-01 00:00:00
abstract：:Doxorubicin and daunorubicin, the anthracycline antitumor agents, were evaluated for their in vitro and in vivo effect on phosphodiesterase (PDE) activity in mouse tissues. Doxorubicin at a concentration of 10(-4)M inhibited cardiac c-AMP (adenosine 3',5', monophosphate) PDE activity 50% of the control whereas in lung...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90641-1
更新日期：1985-02-11 00:00:00
abstract：AIMS:Previous studies have shown that isorhamnetin has anti-adipogenic effects in mouse 3T3-L1 cells. This study was conducted to elucidate the inhibitory mechanisms of isorhamnetin during adipogenic differentiation of human adipose tissue-derived stem cells (hAMSCs). MAIN METHODS:The effect of isorhamnetin on adipoge...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.01.012
更新日期：2010-03-13 00:00:00
abstract：:A completely randomized controlled study based on a rabbit model was designed to study the effect of repetitive ischemic preconditioning (IPC) on a spinal cord ischemic reperfusion injury. Twenty four white adult Japanese rabbits were randomly assigned to one of the 3 groups (n = 8 per group): Group I: sham-operation ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.04.014
更新日期：2006-09-05 00:00:00
abstract：PURPOSE:Doxorubicin, as an effective chemotherapeutic drug, is commonly used for combating various solid and hematological tumors. However, doxorubicin-induced cardiotoxicity is considered as a serious adverse effect, and it limits the clinical use of this chemotherapeutic drug. The use of melatonin can lead to a decre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117173
更新日期：2020-01-15 00:00:00
abstract：:Ventricular hypertrophy is a risk factors for arrhythmias, ischemia and sudden death. It involves cellular modifications leading to a pathological remodeling and is associated with heart failure. The activation of the G protein-coupled estrogen receptor (GPER) mediates beneficial actions in the cardiovascular system. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117211
更新日期：2020-02-01 00:00:00
abstract：:The structure of a new crystal form of leucine-enkephalin has been determined by X-ray diffraction. There are two independent molecules in the asymmetric unit and both have extended peptide backbone conformations with side-chains arranged alternately above and below the backbone planes. The two pentapeptides are hydro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90289-x
更新日期：1987-05-18 00:00:00
abstract：:N(G)-Nitro-L-arginine methyl ester (L-NAME), inhibits the three isozymes of nitric oxide synthase (NOS) in vitro and in vivo. The mechanism of NOS inhibition by L-NAME is uncertain. L-NAME was a time-dependent inhibitor of neuronal NOS (nNOS). Concommitantly, L-NAME was hydrolyzed, non-enzymatically, to N(G)-Nitro-L-a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00140-9
更新日期：1997-01-01 00:00:00
abstract：:Ischemia-reperfusion of organs such as the kidney produces reactive oxygen and free radical species in tissues and leads to injury of intracellular molecules critical to cell homeostasis. Ischemia-reperfusion affects the NADPH-dependent monooxygenase system including P450 system, which is also a source of reactive oxy...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00604-2
更新日期：1997-01-01 00:00:00
abstract：:Behavioral Hypersensitivity (BH) to dopamine agonists occurs following chronic treatment with most neuroleptics including haloperidol. In the present study we observed that the concurrent administration of thioridazine and haloperidol prevented the development of BH. In contrast, another neuroleptic, fluphenazine, coa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90120-7
更新日期：1986-05-12 00:00:00
abstract：:The bradykinin-induced sensitization of cutaneous nociceptors to heat was previously shown to be abolished by cyclooxygenase blockade suggesting that endogenous prostaglandins exerted a heat-sensitizing action. The present study aimed at investigating the effects of exogenous prostaglandin E(2) (PGE(2)) and I(2) (PGI(...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.10.001
更新日期：2007-12-14 00:00:00
abstract：AIMS:Insulin-like growth factor (IGF)-1 is a major mitogenic growth factor for mesangial cells (MCs). Statins slow the progression of chronic kidney disease by affecting inflammatory cell signaling pathways, in addition to improving lipid profile, however, no studies have investigated the effects of fluvastatin on mito...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.02.022
更新日期：2009-05-22 00:00:00
abstract：:We hypothesized that the atheroinhibitory and cardioprotective effects of estrogen may be mediated in part through increased prostacyclin formation by the artery wall. Atherosclerotic abdominal aorta was collected at necropsy from ovariectomized female monkeys fed an atherogenic diet alone or with added Premarin. Basa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01131-6
更新日期：2001-06-15 00:00:00
abstract：:There is a significant need to evaluate the therapeutic potential of natural products and other compounds purported to be hepatoprotective. Acetaminophen-induced liver injury, especially in mice, is an attractive and widely used model for this purpose because it is both clinically relevant and experimentally convenien...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2011.01.025
更新日期：2011-04-25 00:00:00
abstract：:The induction of aldose reductase (AR) mRNA expression and its activity by glucose were investigated in fetal rat aortic smooth muscle (A10) cells. The increase in the expression of AR mRNA was observed at 6 h, and reached a maximum (2.3 fold) at 12 h after exposure to 80 mM glucose. On the other hand, increase in AR ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90480-d
更新日期：1992-01-01 00:00:00
abstract：:Further confirmation that the LHRH/LHRH agonist-induced ovulation in the hypophysectomized (hypx) rat is due to a direct ovarian effect and not mediated by LH release from residual pituitary tissue or other CNS sites is provided by the persistence of this effect despite concomitant median eminence lesion or passive im...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90663-5
更新日期：1984-08-27 00:00:00
abstract：AIMS:Transient ischemia of osteoporotic bones during elective orthopedic surgery or fracture repair carries risks for serious complications, and estrogen loss or replacement has a potential to influence ischemia-reperfusion-induced inflammatory activation. To clarify this, we investigated the periosteal inflammatory ch...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.11.004
更新日期：2011-01-17 00:00:00
abstract：:For the first time the involvement of C-Reactive protein (CRP) in early (acute) and delayed ischemic (IPC) and pharmacological (chemical) preconditioning (CPC) in an in vivo model of rat myocardial infarction was presented. Acute IPC was produced by three 5 minute occlusion (ischemia) periods interspersed with 5 minut...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.12.029
更新日期：2004-07-09 00:00:00
abstract：:Several recent reports indicate that patients with Huntington's Disease (HD) may manifest membrane abnormalities in a wide variety of cells including peripheral blood lymphocytes. In this study, flow cytometry is used in conjunction with the fluorescent membrane probe, 8-anilino-1-naphthalene sulfonate (ANS), to exami...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90165-1
更新日期：1985-02-18 00:00:00
abstract：:Activin A is a member of the transforming growth factor-beta superfamily that exerts its diverse biological effects through bindings to activin specific transmembrane serine/threonine kinase receptors. The fibroblast-mediated contraction of a collagen gel is thought to be a model of part of the wound-repair response a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00480-x
更新日期：2000-03-01 00:00:00
abstract：:Drug-induced refractoriness of alpha-adrenergic receptor-mediated vasoconstriction may be a clinically important phenomenon. We have investigated the possible molecular mechanisms underlying this phenomenon in cultured vascular smooth muscle cells derived from the rabbit aorta. alpha 1-Adrenergic receptors were identi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90824-x
更新日期：1983-10-03 00:00:00
abstract：AIMS:Incidence of stroke increases in postmenopausal women with dangerous consequences. In this study we used zeranol to protect ovariectomized (OVX) rats against cerebral I/R damage and our target is to identify the mechanism of its protection, in addition to investigating whether this mechanism inhibits inflammation ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.04.035
更新日期：2019-06-15 00:00:00
abstract：:The effect of thapsigargin, a specific inhibitor of intracellular Ca(2+)-ATPases, on endothelium-dependent relaxation was studied in the guinea pig thoracic aorta. Thapsigargin (1 nM-1 microM) produced a concentration-dependent relaxation of aortic strips precontracted by phenylephrine (0.1 microM) in the presence and...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90244-w
更新日期：1993-01-01 00:00:00
abstract：:The conversion of beta-carotene to retinal and the succeeding metabolic process of the retinal leading to production of retinol and retinyl esters are the prerequisite for the utilization of beta-carotene as a provitamin A. These processes are participated by beta-carotene cleavage enzyme, retinal reductase and retino...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00311-2
更新日期：1999-01-01 00:00:00
abstract：AIMS:We examined the nature of pharmacological interaction after coadministration of melatonin with clonidine or neostigmine on formalin-induced nociception at the spinal level. Further, the role of melatonin receptor subtypes in melatonin-induced antinociception was clarified. MAIN METHODS:Catheters were inserted int...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.09.028
更新日期：2008-12-19 00:00:00
abstract：:Convulsive seizures are a potential side effect of antidepressant drug treatment and can be produced by all classes of antidepressants. It is also known that some convulsant and anticonvulsant drug actions are mediated by the GABA-receptor chloride-ionophore complex. Drugs acting at this complex appear to induce convu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90107-5
更新日期：1988-01-01 00:00:00
abstract：:Acetyltransferase was isolated by histone-Sepharose affinity chromatography from human cord blood red cells. The enzyme was detected only in very young red cells. The semipurified enzyme and [14C]acetyl-CoA were used to acetylate isolated Hb F tetramer and alpha and gamma subunits. The in vitro acetylated products wer...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90251-2
更新日期：1988-01-01 00:00:00
abstract：:In the early 1970's, some papers appeared reporting an immune response to opiates in animals treated with morphine and in heroin addicts, but further researches failed to confirm these results in humans. The aim of the present work is investigating with a newly developed enzyme immunoassay the existence of specific an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90656-n
更新日期：1993-01-01 00:00:00
