Abstract:
:The low Km GTPase displayed an apparent Km value of 0.2-0.4 microM in P2 fractions from whole mouse brain. The activity of this enzyme ranged from 102 (pmols of GTP hydrolysed per micrograms of protein per min) in the striatum to 39 in the pons-medulla oblongata. Intermediate values were found in other structures, 74-62 in thalamus, hypothalamus, periaqueductal gray matter (PAG), rest of mesencephalon, cortex and spinal cord. The Km also varied throughout the mouse CNS: the spinal cord, striatum and PAG exhibited Km values (0.308-0.271 microM) higher than cortex, thalamus, pons-medulla, hypothalamus and remaining mesencephalon (0.239-0.193 microM). Chronic morphine (3 days) decreased the low Km GTPase activity of PAG (42), whereas it increased the one of thalamus (99). After chronic exposure to the opioid the Km values of the enzyme in striatum (0.193), PAG (0.192) and spinal cord (0.201) diminished, and the ones of hypothalamus (0.357) and rest of mesencephalon (0.287) augmented. The herein reported diversity of low GTPase activity might be due to the presence of different ratios of G alpha types/subtypes in the neural structures studied. As a result of chronic morphine the ratio and/or the functionality of G proteins would be altered in particular areas of mouse CNS.
journal_name
Life Scijournal_title
Life sciencesauthors
Martínez-Peña Y,Garzón Jdoi
10.1016/0024-3205(93)90176-4subject
Has Abstractpub_date
1993-01-01 00:00:00pages
PL123-8issue
16eissn
0024-3205issn
1879-0631pii
0024-3205(93)90176-4journal_volume
52pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Nitric oxide (NO) is a potent regulator in the cardiovascular system; it is generated by the nitric oxide synthase (NOS) family of proteins. NO produced in endothelial cells plays a crucial role in vascular functions. The aim of this study was to clarify the effect of diabetes on aortic NO synthesis in a model of gene...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.02.010
更新日期:2005-07-15 00:00:00
abstract::Deficiencies in cellular cyclic AMP (cAMP) and nitric oxide (NO) production are thought to be involved in the pathogenesis of diabetic neuropathy. We used a human neuroblastoma cell line, SH-SY5Y, to investigate the effect of cilostazol, a specific cAMP phosphodiesterase inhibitor, on NO production and Na+, K+-ATPase ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00379-3
更新日期:1999-01-01 00:00:00
abstract::This study investigated the effects of captopril, hydralazine and losartan on the locomotor activity, tailflick and hot plate latencies in spontaneously hypertensive rats (SHR) and their genetic controls the Wistar-Kyoto rat (WKY). The increased hot plate latencies normally exhibited by the spontaneously hypertensive ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00043-6
更新日期:1995-02-17 00:00:00
abstract:AIMS:Brain injuries based on their causes are divided into two categories, TBI and NTBI. TBI is caused by damages such as head injury, but non-physical injury causes NTBI. Prolactin is one of the blood factors that increase during brain injury. It has been assumed to play a regenerative role in post-injury recovery. M...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118547
更新日期:2020-12-15 00:00:00
abstract::Liver fibrosis is a global health issue that causes morbidity and mortality with no currently available treatment. It has been shown that low dose paclitaxel (PTX) can stabilize microtubules and inhibit the profibrotic transforming growth factor-beta 1 (TGF-β1) signaling pathway. In this study the effect of treatment ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.09.037
更新日期:2018-10-15 00:00:00
abstract::According to our previously proposed auto-addiction hypothesis of chronic anorexia nervosa, patients become addicted to an initial period of dieting through endogenous opioid mediated mechanisms. Morphine causes hyperactivity and anorexia in the mouse, symptoms of anorexia nervosa but responses opposite to those of mo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90457-7
更新日期:1987-08-10 00:00:00
abstract:AIMS:We herein investigated the inducibility of cytochrome P450 1A1 (CYP1A1) by Δ(9)-tetrahydrocannabinol, cannabidiol (CBD), and cannabinol, three major phytocannabinoids, using human hepatoma HepG2 cells. MAIN METHODS:The expression of CYP1A1 and the aryl hydrocarbon receptor (AhR) was measured by a quantitative rea...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.07.007
更新日期:2015-09-01 00:00:00
abstract::Neurotensin (NT) administered intracerebroventricularly (i.c.v.) to rats, blocks intestinal transit (tested by charcoal meal) in linear relation to the log of the doses within the range of 0.6-2.5 nmoles/rat. NT in this test is about 40 times more active than morphine (M) and 6 times less active than dermorphin (DM) o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90547-7
更新日期:1983-01-01 00:00:00
abstract::In the present investigation, Fischer-344 rats were trained to discriminate 10.0 mg/kg of ibogaine from water using a pretreatment time of 60 minutes. Analysis of dose response data generated an ED50 of 4.6 mg/kg. The time course of the ibogaine (10.0 mg/kg) cue was also determined. The stimulus reached a maximum leve...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00703-5
更新日期:1997-01-01 00:00:00
abstract::The effect of i.v. injection of various doses of naloxone (NAL) on plasma adrenocorticotropin (ACTH) and prolactin (Prl) in conscious animals bearing an indwelling intrajugular catheter was assessed. The effects were evaluated in animals which were left undisturbed and in others subjected to either restraint or ether ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90426-8
更新日期:1989-01-01 00:00:00
abstract::3-methylglutaconic (MGT), 3-methylglutaric (MGA) and occasionally 3-hydroxyisovaleric (OHIVA) acids accumulate in a group of diseases known as 3-methylglutaconic aciduria (MGTA). Although the clinical presentation of MGTA is mainly characterized by neurological symptoms, the mechanisms of brain damage in this disease ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.12.024
更新日期:2008-03-12 00:00:00
abstract::Nowadays, endocrine disrupting chemical pollution has become one of the major concerns due to the potential role of these chemicals in provoking endocrine disorders especially type 2 diabetes. As a widespread endocrine disrupting chemical, Bisphenol A, with modest estrogenic activity can exert its detrimental effects ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.10.044
更新日期:2018-12-01 00:00:00
abstract::The ontogeny and regulation by isoproterenol of type II thyroxine 5'-deiodinase, N-acetyltransferase, and hydroxyindole-O-methyltransferase activities were studied in the rat Harderian gland. Both 5'-deiodinase and N-acetyltransferase activities exhibited maximal values at the first week of age. These activities gradu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90510-x
更新日期:1990-01-01 00:00:00
abstract::The effect of changes in carotid sinus perfusion pressure on plasma immunoreactive atrial natriuretic peptide (IR-ANP) was examined in anaesthetized rabbits, and the role of arterial pressure in mediating the changes in IR-ANP was assessed. Plasma IR-ANP was significantly greater (101.7 +/- 24.3 pg ml-1) when carotid ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90355-x
更新日期:1989-01-01 00:00:00
abstract::To assess the effect of pharmacological dose of melatonin on bone metabolism in ovariectomized rats, urinary deoxypyridinoline (a marker of bone resorption) and calcium excretion, circulating levels of calcium, phosphorus and bone alkaline phosphatase activity (a marker of bone formation), and bone mineral density (BM...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01431-x
更新日期:2001-12-21 00:00:00
abstract::The present paper describes the role of conjugating enzymes in the development of hepatotoxicity after administration of repeated doses of a novel monoamine oxidase type-A (MAO-A) inhibitor, (5R)-3-[2-(( 1S)-3-cyano-1-hydroxypropyl)benzothiazol-6-yl]-5-methoxymethyl-2-oxazolidinone (E2011). The effects of pretreatment...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01037-2
更新日期:2001-05-04 00:00:00
abstract::Circadian rhythms might be involved in addictive behaviors. The pineal secretory product melatonin decreases cocaine sensitization in rats; mice mutant for the critical melatonin-synthesizing enzyme, arylalkylamine N-acetyltransferase (AANAT), exhibit altered behaviors. We hypothesized that AANAT/melatonin system, whi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01559-x
更新日期:2002-05-10 00:00:00
abstract::Bradykinin receptor stimulation results in G-protein-coupled phospholipase activation, initiating protein kinase C (PKC) stimulation and cytosolic free Ca2+ concentration ([Ca2+]i) rises as signalling pathways. Using Rb+ as a tracer for K+, we have studied the mechanisms involved in bradykinin-stimulated Rb+ influx in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00530-9
更新日期:1996-01-01 00:00:00
abstract::The aim of this study was to verify, by means of functional methods, whether the circadian rhythm changes adrenergic response patterns in the epididymal half of the vas deferens isolated from control rats as well as from rats submitted to acute stress. The experiments were performed at 9:00 a.m., 3:00 p.m., 9:00 p.m.,...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.02.012
更新日期:2005-08-26 00:00:00
abstract::Following a report that nortriptyline was found useful in the control of enuresis in adults, presumably as an anticholinergic, its likely mechanism of action and apparent bladder specificity have now been investigated in vitro. The ratios of anticholinergic potencies (reciprocal of dissociation contents, Ki) for four ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90093-2
更新日期:1987-05-04 00:00:00
abstract:AIMS:Dysfunction of the Hippo-Yes-Associated Protein (YAP) signaling pathway is known to be associated with hepatocellular carcinoma (HCC). Evodiamine (Evo), a plant-derived bioactive alkaloid, exerts inhibitory effects on cancer. However, the precise influence of Evo on HCC and its potential effects on Hippo-YAP signa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117424
更新日期:2020-06-15 00:00:00
abstract::Homocysteine (Hcy), an intermediate in methionine metabolism, has been proposed to be involved in hepatic fibrogenesis. Impaired liver function can alter Hcy metabolism. The aim of the present study was to determine plasma Hcy alterations in acute obstructive cholestasis and the subsequent biliary cirrhosis. Cholestas...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.12.009
更新日期:2005-04-08 00:00:00
abstract::The content of lactic acid in red blood cells, plasma, and Ehrlich ascites tumor cells were measured by a gas-liquid chromatography using a column with a terephtalic acid support coated with polyethylene glycol-6000. The lactic acid contents were directly determined in aqueous samples, because they were converted to a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90407-g
更新日期:1992-01-01 00:00:00
abstract:AIMS:In this study, we investigated the effects of Bax-inhibiting peptide (Bip)-V5, an anti-apoptosis membrane-permeable peptide, on the 6-hydroxydopamine (6-OHDA) induced Parkinson's disease (PD) model rats. MATERIALS AND METHODS:Rats were randomly divided into five groups: Control, 6-OHDA only, Vehicle+6-OHDA, zVAD+...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.11.019
更新日期:2016-01-01 00:00:00
abstract::Accumulation of excess fluoride in the environment poses serious health risks to plants, animals, and humans. This endangers human health, affects organism growth and development, and negatively impacts the food chain, thereby affecting ecological balance. In recent years, numerous studies focused on the molecular mec...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.02.001
更新日期:2018-04-01 00:00:00
abstract:AIMS:Septic shock, the severe form of sepsis, is associated with development of progressive damage in multiple organs. Kidney can be injured and its functions altered by activation of coagulation, vasoactive-peptide and inflammatory processes in sepsis. Endothelin (ET)-1, a potent vasoconstrictor, is implicated in the ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.03.013
更新日期:2014-05-02 00:00:00
abstract::In a series of in vitro experiments we characterised the relationship between DNA distribution in the G1, S and G2/M phases of cell cycle and PDE and GST activity in CaCo-2 cells. The DNA distribution in CaCo-2 cells, was assessed by flow cytometry, with fluorescent dyes at different time points of culture. The expone...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00169-7
更新日期:1995-01-01 00:00:00
abstract::We have developed an assay for serotonin (5-HT) stimulation of adenylate cyclase activity in membranes from adult guinea pig hippocampus. The response to 5-HT is concentration-dependent, with an EC50 of 0.01 microM, a shallow slope, and mean maximal stimulation of 90% over basal activity. The response to 5-HT is GTP-d...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90763-4
更新日期:1983-05-16 00:00:00
abstract::Activin A is a member of the transforming growth factor-beta superfamily that exerts its diverse biological effects through bindings to activin specific transmembrane serine/threonine kinase receptors. The fibroblast-mediated contraction of a collagen gel is thought to be a model of part of the wound-repair response a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00480-x
更新日期:2000-03-01 00:00:00
abstract::We reported previously that acetylcholine (ACh)-induced endothelium-dependent relaxation of rat mesenteric microvessels depended both on nitric oxide (NO) and on a charybdotoxin (CTX)-sensitive endothelium-derived hyperpolarizing vasodilator. Cytochrome P450 (CYP)-dependent arachidonic acid metabolites act in some sys...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00413-6
更新日期:2000-02-11 00:00:00