Abstract：
:Retinoids have been shown the most powerful inducers of transglutaminase activity, a well known marker of differentiation. In this work, we tested the effects of all-trans retinoic acid and EGF, used alone or in combination, on transglutaminase activity in a squamous, epithelial carcinoma cell line, HEp-2. We demonstrated that nanomolar EGF further enhances transglutaminase activity previously induced by all-trans retinoic acid. Confocal laser scanning microscopy revealed functional changes in transglutaminase activity localisation, at first restricted to the outermost region of cytosol, then diffused both in the membrane region and extracellular space. RT-PCR showed the presence of mRNA transcripts of different transglutaminases (1, 2, 3). Transglutaminase 2 expression was increased by either all-trans retinoic acid or EGF, and further up-regulated by the simultaneous addition of both substances. These effects were confirmed by Western blotting with transglutaminase 2 specific antibody. The results obtained by combined use of retinoic acid and EGF suggest that transglutaminase activity and expression are differently regulated, and that EGF-signalling can be involved in differentiation of epithelial carcinoma cells induced by retinoids.
journal_name
Life Scijournal_title
Life sciencesauthors
Caccamo D,Currò M,Cannavò G,Ientile Rdoi
10.1016/j.lfs.2004.04.047subject
Has Abstractpub_date
2004-10-15 00:00:00pages
2643-52issue
22eissn
0024-3205issn
1879-0631pii
S0024-3205(04)00662-9journal_volume
75pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Ischemia-reperfusion of organs such as the kidney produces reactive oxygen and free radical species in tissues and leads to injury of intracellular molecules critical to cell homeostasis. Ischemia-reperfusion affects the NADPH-dependent monooxygenase system including P450 system, which is also a source of reactive oxy...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00604-2
更新日期：1997-01-01 00:00:00
abstract：:Thymol induced superoxide production (O2-) by blood leukocytes was examined in various primates including man. Leukocytes of chimpanzee and hamadryas baboon cells showed only 35% of the maximal O2- production rate obtained in human cells, and those of the Japanese monkey and orang-utan failed to respond. In contrast, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90735-1
更新日期：1987-09-28 00:00:00
abstract：:Acute myocardial infarction is associated with high rates of morbidity and mortality and can cause irreversible myocardial damage. Timely reperfusion is critical to limit infarct size and salvage the ischemic myocardium. However, reperfusion may exacerbate lethal tissue injury, a phenomenon known as myocardial ischemi...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118628
更新日期：2021-01-01 00:00:00
abstract：:NMDA receptors are involved in the modulation of neuropathic pain behavior and central sensitization. In vivo, this is mostly demonstrated by the use of specific antagonists such as MK801. Because studies evaluating the direct impact of spinal NMDA in neuropathic pain models are lacking, we performed a series of exper...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.10.068
更新日期：2005-06-10 00:00:00
abstract：:The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.02.023
更新日期：2006-07-10 00:00:00
abstract：:Met-enkephalin, administered microiontophoretically, produced a greater increase in firing in cells in area CA 3-4 in the hippocampus of both young and aged Fisher 344 rats than it did in the CA 1 area. Furthermore, the effect of met-enkephalin on neuronal firing rates was not as great in old rats as it was in young r...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90360-2
更新日期：1987-01-12 00:00:00
abstract：:The bone marrow is home to mesenchymal stem cells (MSCs) that are able to differentiate into many different cell types. The effect of hyperbaric oxygen (HBO) on MSCs is poorly understood. Placental growth factor (PlGF) is an attractive therapeutic agent for stimulating revascularization of ischemic tissue. HBO has bee...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.05.005
更新日期：2008-07-04 00:00:00
abstract：:The effects of thioperamide, an H3 antagonist, and histamine H1 and H2 antagonists (s.c.) on morphine (s.c. or i.c.v.)- and U-50,488H (i.c.v.)-induced antinociception in male ddY mice were examined using the hot-plate (55 degrees C) test. Thioperamide significantly inhibited morphine-induced antinociception, but not U...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00589-3
更新日期：1994-01-01 00:00:00
abstract：AIM:Up-regulation of inflammasome proteins was reported in dystrophin-deficient muscles. However, it remains to be determined whether inflammasome activation plays a role in the pathogenesis of Duchenne muscular dystrophy. This study was therefore set out to investigate whether genetic disruption of the inflammasome pa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118069
更新日期：2020-09-15 00:00:00
abstract：:Estrogen has diverse effects on the vasculature, such as vasodilation, endothelial growth and inhibition of vascular smooth muscle cell (VSMC) proliferation and migration. However, little is known about the genes that are regulated by estrogen in the vascular wall. Wistar rats were ovariectomized or sham-operated (Sha...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.03.004
更新日期：2004-07-23 00:00:00
abstract：:Acute ethanol treatment in vivo (i.p. injection of 3.5 g ethanol/Kg B. wt.) stimulated the release of beta-endorphin like peptides by the pituitary gland as was indicated by the increased content of beta-endorphin like immunoreactivity (beta-EPLIR) in the plasma. Furthermore, a significant decrease in the anterior lob...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90247-5
更新日期：1987-01-05 00:00:00
abstract：:TNF production has been studied in peritoneal macrophages and splenic T cells of Arctic Yakutian ground squirrel (Citellus Undulatus Pallas) in hibernating and awake animals in winter and in prehibernating autumn as well as in active euthermic spring-summer animals. A high level of TNF production in macrophages of gro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00698-6
更新日期：2000-01-01 00:00:00
abstract：:The role of Na+ and Ca2+ channels in glutamate-mediated hypoxic injury was investigated in slices of the rat cerebral cortex. Hypoxic injury was determined by mitochondrial reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide after exposure of brain slices to 30-min of hypoxia/glucose deprivation fo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00814-6
更新日期：2000-09-29 00:00:00
abstract：:The melanocortins are a family of bioactive peptides derived from proopiomelanocortin. Those peptides, included among hormones and comprising ACTH, alpha-MSH, beta-MSH and gamma-MSH, are best known mainly for their physiological effects, such as the control of skin pigmentation by alpha-MSH, and ACTH effects on pigmen...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(03)00349-7
更新日期：2003-07-04 00:00:00
abstract：:The purpose of this study was to evaluate the induction of oxidative stress in heart and erythrocytes from rats with abdominal aorta coarctation (Coa) compared with sham-operated normotensive controls (Sham). The group of Coa animals developed myocardial hypertrophy, showing heart homogenates markedly increased levels...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.02.004
更新日期：2006-07-04 00:00:00
abstract：:To examine the physiological significance of plasma ghrelin in generating pulsatile growth hormone (GH) secretion in rats, plasma GH and ghrelin levels were determined in freely moving male rats. Plasma GH was pulsatilely secreted as reported previously. Plasma ghrelin levels were measured by both N-RIA recognizing th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00147-4
更新日期：2003-04-18 00:00:00
abstract：:Peroxisome proliferator-activated receptor (PPAR) and retinoid x receptor (RXR) play important roles in fatty acid metabolism. The present study examined the regulation of retinoic acid receptor (RAR alpha, beta, and gamma), RXR (alpha, beta, and gamma), PPAR, cytochrome P450 2E1 (CYP2E1), catalase, and beta-actin gen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00953-8
更新日期：1995-01-01 00:00:00
abstract：:Ro41-0960 is a potent, fluorine containing COMT inhibitor which has be en reported to cross the blood brain barrier and to inhibit COMT in the brain. It is structurally similar to Ro40-7592 which is currently undergoing clinical trials in Parkinson's disease. Positron emission tomographic (PET) studies in baboon using...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02277-5
更新日期：1996-01-01 00:00:00
abstract：:Leptin regulates glucose, lipid and energy homeostasis as well as feeding behavior, serving as a bridge between peripheral metabolically active tissues and the central nervous system (CNS). Indeed, this adipocyte-derived hormone, whose circulating levels mirror fat mass, not only exerts its anti-obesity effects mainly...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2015.05.012
更新日期：2015-11-01 00:00:00
abstract：:New types of alpha1-adrenoceptor antagonists (tamsulosin, KMD-3213 and JTH-601) are currently receiving a great deal of attention, especially in terms of developing effective therapeutic agents to treat bladder outlet obstruction with less side effects, such as postural hypotension, in patients with benign prostatic h...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00111-8
更新日期：1998-01-01 00:00:00
abstract：:Immunization with plasmid DNA encoding antigenic proteins elicits both antibody and cell-mediated immune responses. This method of producing the protein antigens of interest directly in host cells can provide appropriate tertiary structure for the induction of conformationally specific antibodies, and also facilitates...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(96)00502-4
更新日期：1997-01-01 00:00:00
abstract：:Effects of hexamethonium (C6) on the excitability of sympathetic ganglion cells were examined by means of intracellular recording. When DC currents were injected, high concentrations of C6 significantly augmented the repetitive firing of the cells without any change in threshold voltage for initiation of the spike. Th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90324-8
更新日期：1985-06-17 00:00:00
abstract：:Clinical data indicate that hydroalcoholic extracts of Hypericum perforatum might be as valuable as conventional antidepressants in mild-to-moderate depression, with fewer side effects. One clinical trial using two extracts with different hyperforin contents indicated it as the main active principle responsible for th...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2004.04.005
更新日期：2004-07-16 00:00:00
abstract：:According to recent epidemiological reports, almost 40% of American population use complimentary and alternative medicine (CAM) during their lifetime. Patients detected with HIV or cancer often consume herbal products especially St. John's wort (SJW) for antidepressants in combination with prescription medicines. Such...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2005.12.010
更新日期：2006-03-27 00:00:00
abstract：:Catecholamine uptake into PC12 cells, in the presence and absence of ATP, was measured in a bicarbonate-buffered Krebs Ringer. ATP enhanced significantly the uptake in a dose-dependent manner with maximal uptake at 1-3 mM. ATP increased the Vmax of uptake by 3-5 fold for both dopamine and norepinephrine, without a sig...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00128-s
更新日期：1995-01-01 00:00:00
abstract：AIMS:The aim of this study was to investigate the effects of a mood stabilizer lamotrigine (LMG) on learning and memory processes using Morris water maze (MWM), modified elevated plus maze (mEPM) and passive avoidance (PA) tests. MAIN METHODS:Balb-c mice were used. LMG were injected (20; 40mg/kg, intraperitoneal, i.p....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.020
更新日期：2012-12-17 00:00:00
abstract：:Central dopaminergic (DA) function in children was assessed by monitoring plasma-free homovanillic acid (pHVA) levels after brief (18 hour) administration with debrisoquin sulfate, a peripherally active antihypertensive agent that blocks peripheral, but not central, HVA production. Brief debrisoquin administration res...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90239-0
更新日期：1986-03-17 00:00:00
abstract：:Immunoreactive- (IR-) somatostatin (SRIF), neuropeptide Y (NPY) and corticotropin-releasing factor (CRF) contents were investigated in the brain of tremor rats with absence-like seizure and spontaneously epileptic rats (SER), which is a genetically defined double-mutant (zi/zi, tm/tm) obtained by mating zitter homozyg...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00302-m
更新日期：1995-01-01 00:00:00
abstract：AIMS:Osteoclasts, the unique bone-resorbing polykaryons, are responsible for many bone-destructive diseases, such as osteoporosis and rheumatoid arthritis. Hence, the regulation of osteoclast formation is considered a potential therapeutic approach for these diseases. In this study, we investigated the effect of a nove...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.009
更新日期：2012-11-02 00:00:00
abstract：:Bile duct ligation (BDL) in rats induces portal fibrosis. This process has been linked to changes in the oxidative state of the hepatic cells and in the production of nitric oxide. Our objective was to find possible temporal connections between hepatic redox state, NO synthesis and liver injury. In this work we have c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.01.017
更新日期：2004-06-25 00:00:00
