Expression and characterization of a functional single-chain variable fragment (scFv) protein recognizing MCF7 breast cancer cells in E. coli cytoplasm.

abstract：:The variable numbers of tandem repeats (VNTR) locus D1S80, located on chromosome 1 (1p35-36), has a repeat unit 16 bp in length, and different numbers of these repeat units have been observed for populations of different origins and ethnicity. We used a molecular identification method based on capillary electrophoresi...

journal_title：Biochemical genetics

authors： Köseler A,Atalay A,Atalay EO

更新日期：2009-08-01 00:00:00

abstract：:To elucidate the phylogeny of the genus Paramesotriton (Caudata: Salamandridae), we investigated three mitochondrial DNA gene fragments (1207 bp in total) of cytochrome b, ND2, and ND4 for its six recognized species. The phylogenetic relationships within Paramesotriton were reconstructed by maximum parsimony (MP) and ...

journal_title：Biochemical genetics

authors： Lu S,Yuan ZG,Pang J,Yang D,Yu F,McGuire P,Xie F,Zhang YP

更新日期：2004-06-01 00:00:00

abstract：:Because alternative RNA splicing regulation in the testis is prevalent, we explored testes of Sprague-Dawley rats for existence of alternatively spliced colony-stimulating factor 1 receptor (CSF-1R) mRNA. Using RT-PCR and sequencing, we identified a variant of CSF-1R mRNA that was 284 bp shorter than the full-length C...

journal_title：Biochemical genetics

authors： Su H,Wang Y,Söder O,Hou M

更新日期：2014-06-01 00:00:00

abstract：:We describe a simple method for locating tryptophanyl-tRNA synthetase (E.C. 6.1.1.2) on cellulose acetate gels (Cellogel) following electrophoresis. Employing electrophoretic conditions which result in the separation of mouse and human tryptophanyl-tRNA synthetase, we have analyzed extracts of a number of independentl...

journal_title：Biochemical genetics

authors： Denney RM,Craig IW

更新日期：1976-02-01 00:00:00

abstract：:The murine polycystic kidney disease gene, pcy, is an autosomal recessive trait located on chromosome 9. To determine the genetic locus of pcy, 222 intraspecific backcross mice were obtained by mating C57BL/6FG-pcy and Mus molossinus. Restriction fragment length polymorphism analysis of 70 of the 222 backcross progeny...

journal_title：Biochemical genetics

authors： Nagao S,Watanabe T,Ogiso N,Marunouchi T,Takahashi H

更新日期：1995-12-01 00:00:00

abstract：:Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (G...

journal_title：Biochemical genetics

authors： Wagner S,McCracken J,Cole S,Laible G

更新日期：2010-12-01 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency was identified in two children who were studied because of hemolytic episodes. The electrophoretic and kinetic properties of the mutant enzymes allowed us to conclude that both of them were new variants. They were named G6PD Guantánamo and G6PD Caujerí. ...

journal_title：Biochemical genetics

authors： Gutiérrez A,García M,Estrada M,Quintero I,González R

更新日期：1987-04-01 00:00:00

abstract：:beta-Glucuronidase (GUS) has become an important enzyme model for the genetic study of molecular disease, enzyme realization, and therapy, and for the biogenesis and function of the lysosome and lysosomal enzymes. The genetics of human beta-glucuronidase was investigated utilizing 188 primary man-mouse and man-chinese...

journal_title：Biochemical genetics

authors： Lalley PA,Brown JA,Eddy RL,Haley LL,Byers MG,Goggin AP,Shows TB

更新日期：1977-04-01 00:00:00

abstract：:An electrophoretic analysis of 12 allozyme systems (14 loci, 40 alleles) was performed in the rodent genera Oryzomys and Oligoryzomys, in order to determine the levels of genetic variability within and among populations and species. One hundred and fifty-five individuals from 16 populations of Oryzomys russatus, Or. a...

journal_title：Biochemical genetics

authors： Perini MV,Weimer TA,Callegari-Jacques SM,Mattevi MS

更新日期：2004-10-01 00:00:00

abstract：:Non-alcoholic fatty liver disease is one of the main causes of chronic liver disease and therefore is currently considered a major public health problem. Sirtuin 1 (SIRT1) is an NAD-dependent deacetylase enzyme that contributes in the regulation of metabolic processes and protects against lipid accumulation in hepatoc...

journal_title：Biochemical genetics

authors： Borji M,Nourbakhsh M,Shafiee SM,Owji AA,Abdolvahabi Z,Hesari Z,Ilbeigi D,Seiri P,Yousefi Z

更新日期：2019-08-01 00:00:00

abstract：:CCR5 is a seven-transmembrane G-protein-coupled receptor that binds the CC-chemokines including RANTES, eotaxin, MIP-1alpha and beta. CCR5 serves as an essential coreceptor for cell entry of R5 (macrophage-tropic, nonsyncytium-inducing) strains of HIV-1. To date, four deletions have been found in human and primate ccr...

journal_title：Biochemical genetics

authors： Zhang CY,Wei JF,Het SH

更新日期：2005-06-01 00:00:00

abstract：:Patterns of lactate dehydrogenase (LDH) isozymes of tissues from various mouse strains were examined. An interstrain polymorphism for LDH isozymes of liver was established. One phenotype (CBA/Lac and AKR/J mice) yielded a five-banded LDH pattern, another phenotype (DBA/1J, DBA/2J, C57BL/6J and C3H/He) showed a three-b...

journal_title：Biochemical genetics

authors： Khlebodarova TM,Serov OL

更新日期：1980-10-01 00:00:00

abstract：:There are three major groups of domestic cattle in East and Southeast Asia: European cattle, Zebu cattle, and Bali cattle. Ten restriction enzymes were used to analyze restriction site variants in the mitochondrial DNA (mtDNA) in 178 individuals belonging to these three groups of cattle. The results indicate that each...

journal_title：Biochemical genetics

authors： Kikkawa Y,Amano T,Suzuki H

更新日期：1995-02-01 00:00:00

abstract：:Environmentally induced flax genotrophs L and S show heritable shifts in the relative mobilities of peroxidase, esterase, and acid phosphatase isozymes, plus a number of nonspecific glycoproteins. All L isozymes migrated faster than corresponding S isozymes in 10% acrylamide gels. Various aspects of these shifts are r...

journal_title：Biochemical genetics

authors： Fieldes MA,Tyson H

更新日期：1984-02-01 00:00:00

abstract：:Gli-D1-encoded omega gliadins of bread wheats show little variation; their electrophoretic patterns can be classified into two main groups which broadly resemble the patterns found in the cultivars Chinese Spring and in Cheyenne. B and D subunits of low molecular weight glutenin encoded by the chromosome 1D loci Glu-D...

journal_title：Biochemical genetics

authors： Masci S,Porceddu E,Lafiandra D

更新日期：1991-08-01 00:00:00

abstract：:Heat shock proteins (HSPs), members of molecular chaperones families fulfill essential roles under normal conditions and provide protection and adaptation during and after stress. Among different HSPs, HSP70 kDa family of proteins is most abundant and well-studied in human and mouse but has not yet been characterized ...

journal_title：Biochemical genetics

authors： Tripathy K,Sodhi M,Kataria RS,Chopra M,Mukesh M

更新日期：2020-08-25 00:00:00

abstract：:DNA repair systems are responsible for maintaining the integrity of the genome and have a critical role in protecting against mutations that can lead to cancer. DNA repair gene products of ERCC1 and ERCC2/XPD are involved in the nucleotide excision repair pathway. The allele frequencies of the polymorphisms ERCC1 G190...

journal_title：Biochemical genetics

authors： Yin J,Li J,Vogel U,Wang H

更新日期：2005-10-01 00:00:00

abstract：:Scirpophaga incertulas, commonly referred to as yellow stem borer, is a predominant pest of rice causing serious losses in its yield. Genetic variation among populations of Scirpophaga incertulas collected from 28 hotspot locations in India was examined using the randomly amplified polymorphic DNA-polymerase chain rea...

journal_title：Biochemical genetics

authors： Kumar LS,Sawant AS,Gupta VS,Ranjekar PK

更新日期：2001-02-01 00:00:00

abstract：:In corn seedlings (Zea mays L.) homozygous for the mutation gl5, the surface waxes are characteristically altered. In this mutant the main wax constituents (83.5%) are aldehydes while in the normal waxes alcohols predominate (62.7%). Moreover, in the normal waxes aldehydes and alcohols are made up mainly of the C32 te...

journal_title：Biochemical genetics

authors： Bianchi G,Avato P,Salamini F

更新日期：1978-10-01 00:00:00

abstract：:Human cells irradiated with UV light synthesize lower molecular weight DNA than unirradiated cells. This reduction in molecular weight is greater in xeroderma pigmentosum (XP) cells than in normal cells. The molecular weight of DNA is further reduced by the addition of caffeine to XP cells. By several hours after irra...

journal_title：Biochemical genetics

authors： Minka DF,Nath J

更新日期：1981-04-01 00:00:00

abstract：:Chronic myeloid leukemia (CML) is a hematopoietic stem cell malignancy characterized by the expression of the BCR-ABL1 fusion gene with different chimeric transcripts. Despite the crucial impact of constitutively active tyrosine kinase in CML pathogenesis, aberrant DNA methylation of certain genes plays an important r...

journal_title：Biochemical genetics

authors： Behzad MM,Shahrabi S,Jaseb K,Bertacchini J,Ketabchi N,Saki N

更新日期：2018-06-01 00:00:00

abstract：:Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patter...

journal_title：Biochemical genetics

authors： Yee EY,Zainuddin ZZ,Ismail A,Yap CK,Tan SG

更新日期：2013-10-01 00:00:00

abstract：:UMP synthase was characterized biochemically in dairy cattle heterozygous for a deficiency of this enzyme. Both activities comprising this bifunctional enzyme are decreased, with OMP decarboxylase more affected than orotate phosphoribosyltransferase. Immunotitration of UMP synthase activity revealed the presence of th...

journal_title：Biochemical genetics

authors： Harden KK,Robinson JL

更新日期：1987-08-01 00:00:00

abstract：:The effects of environmental 2-propanol on the in vivo properties of Drosophila alcohol dehydrogenase (E.C. 1..1.1.1.) are presented. Exposed flies were found to exhibit a significant decrease in ADH specific activity with a concomitant increase in the enzyme's relative in vivo stability and concentration. The possibl...

journal_title：Biochemical genetics

authors： Anderson SM,McDonald JF

更新日期：1981-04-01 00:00:00

abstract：:To investigate the sex differences of crossing-over in Rana japonica, the recombination frequencies between two linked loci, LDH-B and MPI, were examined in offspring obtained from 29 matings involving either females or males heterozygous at both loci. No recombinants were found among 303 offspring bred from 6 heteroz...

journal_title：Biochemical genetics

authors： Sumida M,Nishioka M

更新日期：1994-10-01 00:00:00

abstract：:To determine the prevalence of G6PD deficiency and a Mediterranean mutation among males in southeastern Iran, we studied 1,097 Sistani and Balouch schoolboys. A questionnaire was used to collect demographic data and a history of malaria infection; blood samples were evaluated for G6PD deficiency and the G6PD Mediterra...

journal_title：Biochemical genetics

authors： Miri-Moghaddam E,Mortazavi Y,Nakhaee A,Khazaei Feizabad A

更新日期：2013-02-01 00:00:00

abstract：:A restriction map has been constructed for Anastrepha suspensa mitochondrial DNA. One HaeIII site was found to be polymorphic among individuals in highly inbred colonies and a feral population. Based on mapping information, the polymorphic site was determined to be in the ATPase 6 gene. Primers TK-J-3804 and C3-N-5460...

journal_title：Biochemical genetics

authors： Heath M,Kuhn D,Schnell R,Olano C

更新日期：2002-08-01 00:00:00

abstract：:Oral cavity cancer belongs to head-and-neck squamous cell carcinoma group. The purpose of the study was to assess the levels of certain proteins in a tumour and surgical margin in a group of patients with oral cavity cancer. The levels of DAPK1, MGMT, CDH1, SFRP1, SFRP2, RORA, TIMP3, p16, APC and RASSF1 proteins were ...

journal_title：Biochemical genetics

authors： Strzelczyk JK,Gołąbek K,Cuber P,Krakowczyk Ł,Owczarek AJ,Fronczek M,Choręża P,Hudziec E,Ostrowska Z

更新日期：2017-08-01 00:00:00

abstract：:In this study, the geographic patterns of genetic variation of three rodent species belonging to the tribe Oryzomyini were investigated using the mitochondrial cytochrome b and nuclear IRBP genes in biomes that are undergoing degradation processes to a greater or lesser degree. The samples are from 25 collecting local...

journal_title：Biochemical genetics

authors： Miranda GB,Andrades-Miranda J,Oliveira LF,Langguth A,Mattevi MS

更新日期：2007-12-01 00:00:00

abstract：:A new polymorphism, Pc, has been identified in human saliva. Two proteins, Pc 1 and Pc 2, are determined by alleles Pc1 and Pc2, respectively, which show autosomal codominant inheritance. No null phenotype has been encountered in 225 randomly collected salivas. The frequencies of the two alleles differ in the Black an...

journal_title：Biochemical genetics

authors： Karn RC,Goodman PA,Yu PL

更新日期：1985-02-01 00:00:00