Cytological evidence for DNA chain elongation after UV irradiation in the S phase.

Abstract:

:Human cells irradiated with UV light synthesize lower molecular weight DNA than unirradiated cells. This reduction in molecular weight is greater in xeroderma pigmentosum (XP) cells than in normal cells. The molecular weight of DNA is further reduced by the addition of caffeine to XP cells. By several hours after irradiation, DNA fragments are barely detectable. Cells from excision-proficient and excision-deficient XP patients were studied autoradiographically to produce cytological evidence of DNA chain elongation. Replicate cultures with and without caffeine were synchronized and irradiated with UV light during the S phase. Caffeine was removed in G2, and the cells were labeled with 3H-thymidine. Results showed significantly increased labeling during G2 of excision-deficient XP cells. Labeling was dependent on the time of irradiation and presence of caffeine. The XP variant cells had no increase in labeling for any irradiation time.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Minka DF,Nath J

doi

10.1007/BF00504267

subject

Has Abstract

pub_date

1981-04-01 00:00:00

pages

199-210

issue

3-4

eissn

0006-2928

issn

1573-4927

journal_volume

19

pub_type

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