Genetic and biochemical studies of the highly active esterases A' and B associated with organophosphate resistance in mosquitoes of the Culex pipiens complex.

Abstract:

:The highly active esterases A' and B that cannot be dissociated from OP resistance in Culex pipiens from France and California are shown to have equivalent Km values (2.1 x 10(-6) M/min/mosquito) but different turnover rates (Vm = 2.13 and 0.57 x 10(-6) M/min/mosquito, respectively) and pH for maximum activity. Both enzymes have broad substrate specificities and at least one, esterase A', can hydrolyze OP insecticides. In addition, esterases A' and B are coded by two closely linked genes, Est-3 and Est-2, respectively (0.67 unit of crossing over), located on the same autosome as pl, a locus attributed to linkage group III. The estimated distance between Est-2 and pl was 9.4 units.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Pasteur N,Iseki A,Georghiou GP

doi

10.1007/BF00504256

subject

Has Abstract

pub_date

1981-10-01 00:00:00

pages

909-19

issue

9-10

eissn

0006-2928

issn

1573-4927

journal_volume

19

pub_type

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