Abstract:
:The retinal pigment epithelium (RPE) plays a key role in the maintenance of the normal functions of the retina. Tissue engineering using amniotic membrane as a substrate to culture RPE cells may provide a promising new strategy to replace damaged RPE. We established a method of culturing RPE cells over the amniotic membrane as a support for their growth and transplantation. The transcription of specific genes involved in cellular function of native RPE, including RPE65, CRALBP, VEGF, CD68, and tyrosinase, were then measured using quantitative real-time PCR. Data showed a considerable increase in transcription of RPE65, CD68, and VEGF in RPE cells cultured on amniotic membrane. The amounts of CRALBP and tyrosinase transcripts were not affected. This may simply indicate that amniotic membrane restricted dedifferentiation of RPE cells in culture. The results suggest that amniotic membrane may be considered as an elective biological substrate for RPE cell culture.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Akrami H,Soheili ZS,Sadeghizadeh M,Khalooghi K,Ahmadieh H,Kanavi MR,Samiei S,Pakravesh Jdoi
10.1007/s10528-010-9409-1subject
Has Abstractpub_date
2011-06-01 00:00:00pages
313-22issue
5-6eissn
0006-2928issn
1573-4927journal_volume
49pub_type
杂志文章abstract::The allele, genotype, and haplotype frequencies among 837 Polish Holstein-Friesian cattle were determined at two regulatory indel polymorphisms of the PRNP gene. Allele frequencies at the 23 bp indel promoter polymorphism were 0.622 (del) and 0.378 (ins), with 0.613 and 0.387 in sires and 0.633 and 0.366 in dams. Alle...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-010-9408-2
更新日期:2011-06-01 00:00:00
abstract::Differences in Zn-induced levels of hepatic metallothionein (MT) in inbred strains of the mouse are described. Three low-producing strains, C57 BL/6, C57BL/10, and NIH, are identified, while C3H and CBA display the highest levels of hepatic MT following Zn treatment. These interstrain differences affect not only the l...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02401801
更新日期:1989-04-01 00:00:00
abstract::An electrophoretical analysis of blood plasma proteins of eight Anser and two Branta species was performed. Ten polymorphic proteins in blood plasma pattern were distinguished and described: four prealbumin proteins, albumin, three postalbumin proteins, transferrin, and a single posttransferrin protein. Genus-specific...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00557950
更新日期:1995-04-01 00:00:00
abstract::An enzyme catalyzing the transfer of the glucosyl moiety of UDP-glucose to the 3-hydroxyl group of cyanidin has been demonstrated in petal extracts of Silene dioica mutants with cyanidin-3-O-glucoside in the petals. This transferase activity was also present in young rosette leaves and calyces of these plants. The hig...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484525
更新日期:1978-12-01 00:00:00
abstract::Investigations of an euploid series of castor bean, Ricinus communis L., Consisting of haploid, diploid, and tetraploid individuals, was performed to determine the value of such a series in studying the biochemical consequences of genome multiplication. The effects of euploidization of the nuclear genome on the biosyn...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504367
更新日期:1980-02-01 00:00:00
abstract::Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were determined through trisomic analysis. All 10 genes produced altered allozyme banding patterns in specific F1 trisomics. This served as the primary source of evidence for chromosome locations of Est-5, Icd-1, Acp-1, and Pgd-1. The locations of Amp-...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00561468
更新日期:1988-04-01 00:00:00
abstract::The RAPD method was used to assess the genetic differentiation of brown hare (Lepus europaeus) populations from Central Greece. Greek wild populations were compared with samples from Austria, Poland, Germany, France, and Bulgaria, as well as with reared/released hares to investigate the impact of the releases on the n...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1020260819629
更新日期:2002-10-01 00:00:00
abstract::Stable reciprocal hybrids between Flaveria pringlei (C3) and F. brownii (C4-like) have been produced by standard breeding techniques. There are no differences in the isoelectric focusing patterns of the catalytic subunits of the ribulose-1,5-bisphosphate carboxylase/oxygenase from F. pringlei, F. brownii, or the recip...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02396147
更新日期:1989-10-01 00:00:00
abstract::To determine the genetic structure and variation of Van cats and some other cats, seven enzyme loci were examined using horizontal starch gel electrophoresis. ME bands were observed for the first time in cats. For the enzyme loci CA ( 1 ), SOD, GPI, and GOT, neither the individual Van cats nor the specimens of other c...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-011-9426-8
更新日期:2011-08-01 00:00:00
abstract::Active-site tryptic peptides were isolated from three genetic types of human serum cholinesterase. The active-site peptide was identified by labeling the active-site serine with [3H]diisopropylfluorophosphate. Peptides were purified by high-performance liquid chromatography. Amino acid composition and sequence analysi...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499101
更新日期:1986-06-01 00:00:00
abstract::The tissue distribution of activin receptor-like kinase 7 (Alk7) expression, the signaling ability of Alk7 variants, and Alk7 expression in response to β3-adrenergic receptor activation were examined. Expression levels of Alk7 varied greatly among tissues but were highest in white adipose tissue and brown adipose tiss...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9555-8
更新日期:2013-04-01 00:00:00
abstract::Glycogen synthase, which catalyzes the synthesis of glycogen, is especially important for Old World (Pteropodidae) and New World (Phyllostomidae) fruit bats that ingest high-carbohydrate diets. Glycogen synthase 1, encoded by the Gys1 gene, is the glycogen synthase isozyme that functions in muscles. To determine wheth...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-014-9659-4
更新日期:2014-10-01 00:00:00
abstract::Sequence divergence of mitochondrial COII was analyzed in 50 specimens belonging to five subspecies of Polyura eudamippus (Lepidoptera: Nymphalidae) collected from southern China. There were nine haplotypes in the 405 bp of partial COII sequence. Distribution of the five subspecies was primarily consistent with the sp...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9035-0
更新日期:2006-10-01 00:00:00
abstract::Among several autoimmune diseases, one of the main risk factors is the female gender, and much consideration has been given to the involvement of female hormones in their etiology. B-cell activating factor (BAFF) is a key factor in survival and maturation of B cells and is overexpressed in several autoimmune patients ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-016-9752-y
更新日期:2016-10-01 00:00:00
abstract::The autoregulation of testicular luteinizing hormone (LH) receptors was studied in hypogonadal (hpg/hpg) and normal mice. The basal concentration of LH receptors was more than three-fold higher in hpg/hpg than in normal mice. After injection of hCG, hpg/hpg mice showed a decrease in LH receptor levels which was not ob...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484511
更新日期:1984-06-01 00:00:00
abstract::Melanoma formation in Xiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by the Tu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecular identification of this regulatory locus (R) r...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02426867
更新日期:1991-12-01 00:00:00
abstract::Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six bre...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-008-9152-z
更新日期:2008-06-01 00:00:00
abstract::Species in the genus Oryza (Poaceae) contain 10 genomic types and are distributed in pan-tropics of the world. To explore phylogenetic relationships of Oryza species having the AA-genome, DNA sequences of the chloroplast trnL intron and trnL-trnF spacer, mitochondrial nad1 intron 2, and nuclear internal transcribed sp...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9062-x
更新日期:2007-02-01 00:00:00
abstract::Glucose-6-phosphate dehydrogenase (G6PD) deficiency was identified in two children who were studied because of hemolytic episodes. The electrophoretic and kinetic properties of the mutant enzymes allowed us to conclude that both of them were new variants. They were named G6PD Guantánamo and G6PD Caujerí. ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499316
更新日期:1987-04-01 00:00:00
abstract::The primary structures of the alpha globins from CE/J, DBA/2J, and a stock of Potter's mice were determined to identify the amino acid substitutions associated with the unique isoelectric focusing patterns of these hemoglobins. In addition, the primary structures of the alpha globins from MOL III and PERU mice were st...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484946
更新日期:1982-02-01 00:00:00
abstract::The ratio of cellular RNA and protein content is about 1:1 between phylogenetically diploid and tetraploid species of the teleost family Cyprinidae, but is roughly in proportion to ploidy in species of the teleost order Isospondyli. Enzyme activities do not unequivocally comply with this scheme. These findings are dis...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00485129
更新日期:1976-12-01 00:00:00
abstract::Heat shock proteins (HSPs), members of molecular chaperones families fulfill essential roles under normal conditions and provide protection and adaptation during and after stress. Among different HSPs, HSP70 kDa family of proteins is most abundant and well-studied in human and mouse but has not yet been characterized ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-020-09994-7
更新日期:2020-08-25 00:00:00
abstract::Using two-dimensional gel electrophoresis, we have analyzed proteins from a plasma membrane-enriched fraction from Phycomyces sporangiophores. Specifically, we have compared gels for night-blind mutants and a wild-type strain to find proteins involved in the early steps of the sensory transduction chain for phototropi...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499081
更新日期:1985-06-01 00:00:00
abstract::Single-chain variable fragment (scFv) is one of the most common antibody forms. This report describes the expression of the scFv gene as a soluble protein in Origami DE3 cytoplasm. The purified scFv recognized the epidermal growth factor receptor (EGFRvIII) on the surface of MCF-7 cells. The scFv protein was purified ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9506-4
更新日期:2012-08-01 00:00:00
abstract::Aggrecan is the major component of intervertebral disk matrix proteoglycan with multiple functional domains. To understand the role of aggrecan polymorphism in a part of exon 12 encoding the CS1 domain in lumbar disk degeneration disease, we have analyzed genomic DNA from 71 patients with the disease and 108 healthy i...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-010-9350-3
更新日期:2010-08-01 00:00:00
abstract::To elucidate the phylogeny of the genus Paramesotriton (Caudata: Salamandridae), we investigated three mitochondrial DNA gene fragments (1207 bp in total) of cytochrome b, ND2, and ND4 for its six recognized species. The phylogenetic relationships within Paramesotriton were reconstructed by maximum parsimony (MP) and ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/b:bigi.0000026630.78770.fb
更新日期:2004-06-01 00:00:00
abstract::The rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-013-9584-y
更新日期:2013-08-01 00:00:00
abstract::Three distinct malathion carboxylesterase (MCE) phenotypes have been identified among strains of Lucilia cuprina. The high-activity phenotype shows 1.6- and 33-fold more MCE specific activity than the intermediate- and low-activity phenotypes, respectively. Flies with high MCE activity are 1000-fold more resistant to ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00566064
更新日期:1994-12-01 00:00:00
abstract::In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene appears to play a role in nitrogen catabolite repression. Using the N. crassa nmr gene as a probe, homologous sequences were ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::In vitro DNA:DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid Triticum species ancestral to the B genome of T. turgidum. Unique and repeated sequences from the various Triticum species were separated by hybridization and thermal elution on hydroxyapatite. Uniqu...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00502977
更新日期:1986-02-01 00:00:00