Evaluation of RPE65, CRALBP, VEGF, CD68, and tyrosinase gene expression in human retinal pigment epithelial cells cultured on amniotic membrane.

Abstract:

:The retinal pigment epithelium (RPE) plays a key role in the maintenance of the normal functions of the retina. Tissue engineering using amniotic membrane as a substrate to culture RPE cells may provide a promising new strategy to replace damaged RPE. We established a method of culturing RPE cells over the amniotic membrane as a support for their growth and transplantation. The transcription of specific genes involved in cellular function of native RPE, including RPE65, CRALBP, VEGF, CD68, and tyrosinase, were then measured using quantitative real-time PCR. Data showed a considerable increase in transcription of RPE65, CD68, and VEGF in RPE cells cultured on amniotic membrane. The amounts of CRALBP and tyrosinase transcripts were not affected. This may simply indicate that amniotic membrane restricted dedifferentiation of RPE cells in culture. The results suggest that amniotic membrane may be considered as an elective biological substrate for RPE cell culture.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Akrami H,Soheili ZS,Sadeghizadeh M,Khalooghi K,Ahmadieh H,Kanavi MR,Samiei S,Pakravesh J

doi

10.1007/s10528-010-9409-1

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

313-22

issue

5-6

eissn

0006-2928

issn

1573-4927

journal_volume

49

pub_type

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