Abstract：
:An enzyme catalyzing the transfer of the glucosyl moiety of UDP-glucose to the 3-hydroxyl group of cyanidin has been demonstrated in petal extracts of Silene dioica mutants with cyanidin-3-O-glucoside in the petals. This transferase activity was also present in young rosette leaves and calyces of these plants. The highest glucosyltransferase activity was found in petals of opening flowers of young plants. The enzyme was purified ninetyfold by PVP and Sephadex chromatography. The glucosyltransferase had a pH optimum of 7.5, had a "true Km value" of 4.1 x 10(-4) M for UDP-glucose and 0.4 x 10(-4) M for cyanidin chloride, and was not stimulated by divalent metal ions. Both p-chloromercuribenzoate and HgCl2 inhibited the enzyme activity. Pelargonidin chloride and delphinidin chloride at reduced rates also served as substrates. The enzyme did not catalyze the glucosylation of the 3-hydroxyl group of flavonols or the 5-hydroxyl group of anthocyanins. ADP-glucose could not serve as a glucosyl donor. The results of Sephadex G150 chromatography suggest that the glucosyltransferase can exist as dimer of about 125,000 daltons and as active monomers of 60,000 daltons. The genetic control of the glucosyltransferase activity is discussed.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Kamsteeg J,van Brederode J,van Nigtevecht Gdoi
10.1007/BF00484525subject
Has Abstractpub_date
1978-12-01 00:00:00pages
1045-58issue
11-12eissn
0006-2928issn
1573-4927journal_volume
16pub_type
杂志文章abstract：:DNA repair systems are responsible for maintaining the integrity of the genome and have a critical role in protecting against mutations that can lead to cancer. DNA repair gene products of ERCC1 and ERCC2/XPD are involved in the nucleotide excision repair pathway. The allele frequencies of the polymorphisms ERCC1 G190...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-005-8170-3
更新日期：2005-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504345
更新日期：1979-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554162
更新日期：1989-06-01 00:00:00
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pub_type： 杂志文章
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00486123
更新日期：1976-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02399419
更新日期：1988-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00556214
更新日期：1987-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484880
更新日期：1976-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484692
更新日期：1982-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10528-011-9426-8
更新日期：2011-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504306
更新日期：1979-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10528-005-1503-4
更新日期：2005-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484587
更新日期：1980-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485850
更新日期：1984-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499943
更新日期：1985-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426867
更新日期：1991-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02396156
更新日期：1989-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00554618
更新日期：1994-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02396237
更新日期：1996-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484099
更新日期：1977-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00500133
更新日期：1980-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1018781026170
更新日期：1998-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499081
更新日期：1985-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1018753714783
更新日期：1998-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9607-8
更新日期：2013-10-01 00:00:00
