Abstract:
:Skeletal muscle tissues from many species of salmonid fish are known to exhibit a set of three of five isozymes for A4-type lactate dehydrogenase (L-lactate: NAD oxidoreductase, E.C. 1.1.1.27), but the genetic basis for this isozyme system has not previously been assessed. This isozyme system was purified to homogeneity from salmon (Oncorhynchus tshawytscha) and shown to be composed of two polypeptides. Aalpha and Abeta, in binomial tetrameric combinations. Amino acid analysis revealed that Aalpha and Abeta are closely related but genetically distinct proteins, and thus are coded for the recently duplicated structural loci. Catalytic studies on the purified intact salmon isozyme system and on the isozymically pure Aalpha4 and Abeta4 homotetramers from brown trout (Salmo trutta) revealed no significant differences in catalytic properties among these enzymes, suggesting equivalent catalytic function of Aalpha and Abeta. These results, in combination with studies on polypeptide and isozyme ratios, suggest that one of the duplicated loci in salmon may be drifting toward a nonfunctional state by accumulation of mutations in regulatory DNA rather than in the structural gene itself.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Lim ST,Bailey GSdoi
10.1007/BF00484099subject
Has Abstractpub_date
1977-08-01 00:00:00pages
707-21issue
7-8eissn
0006-2928issn
1573-4927journal_volume
15pub_type
杂志文章abstract::We have examined the linkage relationships between five biochemical markers, Acon-1, Ahd-2, Ahd-c, Akp-1, and Hao-1, and 19 other genetic loci in five breeding combinations. The genetic locus that codes for a recently described aldehyde dehydrogenase in the liver (Ahd-c) has been assigned to linkage group X (LG X). Hy...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00502790
更新日期:1986-04-01 00:00:00
abstract::When the 4-bp Dam recognition sequence was placed between two d(GA)7 tracts, it became severely undermethylated in JM101 Escherichia coli cells compared to other Dam sequences in the same plasmid DNA. This site specific undermethylation was also detected on supercoiled molecules in vitro. Mutational analysis indicated...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02407017
更新日期:1996-06-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-008-9170-x
更新日期:2008-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-011-9434-8
更新日期:2011-10-01 00:00:00
abstract::The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate, pH 3.1) and two-dimensional (first dimension, Al-lactate, pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the alpha zone...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02395516
更新日期:1988-12-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504335
更新日期:1986-08-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章,评审
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更新日期:2015-10-01 00:00:00
abstract::A partial paired gene of Drosophila willistoni containing the paired box and extended homeo box was amplified by PCR and the nucleotide sequence of 1141 bp was determined. Comparison of the paired genes in D. willistoni and D. melanogaster showed that the proportions of identical nucleotide sites in the coding region ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00570122
更新日期:1996-12-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-018-9845-x
更新日期:2018-08-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00486138
更新日期:1981-02-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-011-9480-2
更新日期:2012-06-01 00:00:00
abstract::We have shown in our previous work that in the tetraploid loach Misgurnus fossilis carboxylesterase-2 (E-2) phenotypes of embryos and early larvae are controlled by the alleles of the Est-2 locus. In the present work it has been established that the somatic tissues of many adult fish homozygous for one of the Est-2 al...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484449
更新日期:1983-06-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1018761916601
更新日期:1999-02-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00488466
更新日期:1983-12-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-020-09971-0
更新日期:2020-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-008-9166-6
更新日期:2008-08-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/b:bigi.0000006031.20967.08
更新日期:2003-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-015-9668-y
更新日期:2015-04-01 00:00:00
abstract::An Italian organophosphate-resistant strain of Culex pipiens (Lucca) was found to be polymorphic for elevated and nonelevated esterases. Selection for high esterase activity produced a strain homozygous for elevated esterases A2 and B2. Selection for low activity produced a strain homozygous for nonelevated esterases,...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02426878
更新日期:1993-12-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-005-1503-4
更新日期:2005-04-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-013-9607-8
更新日期:2013-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00485850
更新日期:1984-08-01 00:00:00
abstract::The primary structures of the alpha globins from CE/J, DBA/2J, and a stock of Potter's mice were determined to identify the amino acid substitutions associated with the unique isoelectric focusing patterns of these hemoglobins. In addition, the primary structures of the alpha globins from MOL III and PERU mice were st...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484946
更新日期:1982-02-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-007-9131-9
更新日期:2008-02-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9075-5
更新日期:2007-04-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00483996
更新日期:1981-08-01 00:00:00
abstract::Eight Drosophila melanogaster strains, seven homozygous for AdhF alleles and one for an Adh-null mutant, were compared for ADH activity in males and adult mortality on ethanol-supplemented food. The strains differed considerably in these qualities. A positive correlation was found between ADH activity and LD50. The re...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484218
更新日期:1978-06-01 00:00:00
abstract::A new variant of alcohol dehydrogenase (ADH 7lk) was found in a laboratory stock of Drosophila melanogaster. ADH in this stock had the same electrophoretic mobility as the F variant both on acrylamide and on agar. Activity levels were similar to the levels in F flies at temperature between 15 and 25 C. But while ADH F...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484929
更新日期:1975-10-01 00:00:00
abstract::In this study, we identified porcine single nucleotide polymorphisms (SNPs) by aligning eight sequences generated with two approaches: amplification of 665 intronic regions using one sample from each of eight breeds, including three East Asian pigs, and amplification of 289 3'-UTR regions using two samples from each o...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-011-9486-9
更新日期:2012-06-01 00:00:00