Abstract:
:When the 4-bp Dam recognition sequence was placed between two d(GA)7 tracts, it became severely undermethylated in JM101 Escherichia coli cells compared to other Dam sequences in the same plasmid DNA. This site specific undermethylation was also detected on supercoiled molecules in vitro. Mutational analysis indicated that undermethylation is related to the capacity of the oligopurine tract to adopt the H-DNA conformation. In addition, chemical probing of the cells was consistent with a cellular protein bound to the DNA. Therefore it is likely that the combination of altered DNA conformation and a cellular protein leads to Dam-site protection. We also found that the site-specific undermethylation is detectable in certain E. coli strains only.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Klysik Jdoi
10.1007/BF02407017subject
Has Abstractpub_date
1996-06-01 00:00:00pages
165-78issue
5-6eissn
0006-2928issn
1573-4927journal_volume
34pub_type
杂志文章abstract::Information on genetic variation is essential for conservation and stock improvement programs. Seven dinucleotide microsatellite loci were analyzed to reveal genetic variability in three wild populations (Kella beel, Hakaluki haor, and Shobornokhali beel) and one hatchery population of the freshwater walking catfish, ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-007-9102-1
更新日期:2007-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484739
更新日期:1978-08-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499474
更新日期:1984-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499114
更新日期:1985-02-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484511
更新日期:1984-06-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9555-8
更新日期:2013-04-01 00:00:00
abstract::Human cells irradiated with UV light synthesize lower molecular weight DNA than unirradiated cells. This reduction in molecular weight is greater in xeroderma pigmentosum (XP) cells than in normal cells. The molecular weight of DNA is further reduced by the addition of caffeine to XP cells. By several hours after irra...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504267
更新日期:1981-04-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-009-9274-y
更新日期:2009-12-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499473
更新日期:1984-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-014-9656-7
更新日期:2014-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-007-9139-1
更新日期:2008-04-01 00:00:00
abstract::Patterns of lactate dehydrogenase (LDH) isozymes of tissues from various mouse strains were examined. An interstrain polymorphism for LDH isozymes of liver was established. One phenotype (CBA/Lac and AKR/J mice) yielded a five-banded LDH pattern, another phenotype (DBA/1J, DBA/2J, C57BL/6J and C3H/He) showed a three-b...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00500133
更新日期:1980-10-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00578241
更新日期:1991-02-01 00:00:00
abstract::A novel cDNA fragment was identified from a human fetal brain cDNA library by using the coding sequence of human BRI3 gene (Accession No. NM015379) as bait in a yeast two-hybrid screening. Then by 5'-RACE (rapid amplification of cDNA end) and electronic hybridization, we obtained a 1.9 kb contig which consists of a no...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1013807518791
更新日期:2001-12-01 00:00:00
abstract::Human PQBP-1 is known to interact with triplet repeat disease gene products such as ataxin and huntingtin through their poly-glutamine (poly-Q) tracts. The poly-Q tracts show extensive variation in both the number and the configuration of repeats among species. A surface plasmon resonance assay showed clear interactio...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-011-9473-1
更新日期:2012-04-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-020-10004-z
更新日期:2020-09-29 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9035-0
更新日期:2006-10-01 00:00:00
abstract::ZFY-like genes have been observed in a variety of vertebrate species. Although originally implicated as the primary testis-determining gene in humans and other placental mammals, more recent evidence indicates a role(s) outside that of testis determination. In this study, DNA from five species of fish, Carasius auratu...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484728
更新日期:1978-08-01 00:00:00
abstract::The retinal pigment epithelium (RPE) plays a key role in the maintenance of the normal functions of the retina. Tissue engineering using amniotic membrane as a substrate to culture RPE cells may provide a promising new strategy to replace damaged RPE. We established a method of culturing RPE cells over the amniotic me...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-010-9409-1
更新日期:2011-06-01 00:00:00
abstract::To elucidate the phylogeny of the genus Paramesotriton (Caudata: Salamandridae), we investigated three mitochondrial DNA gene fragments (1207 bp in total) of cytochrome b, ND2, and ND4 for its six recognized species. The phylogenetic relationships within Paramesotriton were reconstructed by maximum parsimony (MP) and ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/b:bigi.0000026630.78770.fb
更新日期:2004-06-01 00:00:00
abstract::Genetic analysis of a polymorphic tissue esterase revealed a new locus (Est-6) with two alleles (Est-6a and Est-6b) on linkage group VI of the rabbit. Est-6 is closely linked to the Est-1,2,4 cluster. Esterase of Est-6 is found in many organs, particularly in liver and small intestine, but not in erythrocytes and seru...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00554543
更新日期:1987-06-01 00:00:00
abstract::The locus for sorbitol dehydrogenase (SORD, E.C. 1.1.1.14) has been shown to segregate with hexosaminidase A and mannose phosphate isomerase in a series of human-Chinese hamster somatic cell hybrids. Cytogenetic analysis supports the assignment to chromosome 15 and further defines the gene locus to the region 15pter l...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484391
更新日期:1980-06-01 00:00:00
abstract::Starch gel electrophoresis and histochemical staining with L-leucylglycyl-glycine revealed genetic polymorphism in peptidase B in cattle erythrocytes. Genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, PepB1 and PepB2. A...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a hematopoietic stem cell malignancy characterized by the expression of the BCR-ABL1 fusion gene with different chimeric transcripts. Despite the crucial impact of constitutively active tyrosine kinase in CML pathogenesis, aberrant DNA methylation of certain genes plays an important r...
journal_title:Biochemical genetics
pub_type: 杂志文章,评审
doi:10.1007/s10528-018-9841-1
更新日期:2018-06-01 00:00:00
abstract::Heat shock proteins (HSPs), members of molecular chaperones families fulfill essential roles under normal conditions and provide protection and adaptation during and after stress. Among different HSPs, HSP70 kDa family of proteins is most abundant and well-studied in human and mouse but has not yet been characterized ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-020-09994-7
更新日期:2020-08-25 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484232
更新日期:1984-04-01 00:00:00
abstract::Mitochondrial DNA (mtDNA) from two breeds of cattle, viz., [Hariana (Bos indicus), Holstein (Bos taurus)] and Indian water buffalo (Bubalis bubalus), was analyzed using 13 restriction endonucleases which recognized an average of about 40 six-base sites. Polymorphism among cattle was detected with six of these enzymes....
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02401421
更新日期:1990-08-01 00:00:00
abstract::The RAPD method was used to assess the genetic differentiation of brown hare (Lepus europaeus) populations from Central Greece. Greek wild populations were compared with samples from Austria, Poland, Germany, France, and Bulgaria, as well as with reared/released hares to investigate the impact of the releases on the n...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1020260819629
更新日期:2002-10-01 00:00:00
abstract::Prolamin mixtures were isolated from oats, rice, normal and high-lysine sorghum, two varieties of pearl millet, two strains of teosinte, and gamma grass and subjected to NH2-terminal amino acid sequence determinations. In each case (except for rice, whose prolamins apparently have blocked or unavailable NH2-terminal r...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00498931
更新日期:1982-12-01 00:00:00