Abstract：
:It is shown that the gene controlling the synthesis of the organ-specific S-esterase of Drosophila virilis ejaculatory bulbs is located on the second chromosome (at approximate position 192.1 +/- map units). The cells of the genital imaginal disks are determined for the synthesis of S-esterase 10-12 hr after the second molt. The organ-specific esterase can be detected after adult emergence only. It is preceded by an increase in RNA content and by enhancement of RNA synthesis in the cells of the ejaculatory bulbs. Interstock differences were found in the level of the activity of S-esterase, which is under the control of the X chromosome, as well as in the time of expression of enzyme activity, which is controlled by the fifth chromosome. It is suggested that the specific phenotypic expression of this enzyme depends on the system of genes with regulatory expression at both the transcriptional and posttranscriptional levels. The genetic control of the synthesis of the S-esterase described is a convenient model for studying mechanisms of gene activity regulation in eukaryotes.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Korochkin LI,Matveeva NM,Kuzin BA,Karasik GI,Maximovsky LFdoi
10.1007/BF00484728subject
Has Abstractpub_date
1978-08-01 00:00:00pages
709-26issue
7-8eissn
0006-2928issn
1573-4927journal_volume
16pub_type
杂志文章abstract：:A novel cDNA fragment was identified from a human fetal brain cDNA library by using the coding sequence of human BRI3 gene (Accession No. NM015379) as bait in a yeast two-hybrid screening. Then by 5'-RACE (rapid amplification of cDNA end) and electronic hybridization, we obtained a 1.9 kb contig which consists of a no...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1013807518791
更新日期：2001-12-01 00:00:00
abstract：:Polymorphic esterase and acetylcholinesterase alleles in the whitefly Bemisia tabaci were studied using electrophoretic and colorimetric assays. The segregation of these alleles between parental and F1 generations provided unequivocal evidence of haplodiploidy in this pest species. Unmated females, heterozygous at a p...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:The East Himalaya-Hengduan Mountains region is the center of diversity of the genus Primula, and P. sikkimensis is one of the most common members of the genus in the region. In this study, the genetic diversity and structure of P. sikkimensis populations in China were assessed using inter-simple sequence repeat (ISSR)...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-007-9131-9
更新日期：2008-02-01 00:00:00
abstract：:The antigenic properties of a number of chromatographically purified primate hemoglobins were compared to those of normal human hemoglobin using a sensitive radioimmunochemical procedure. The degree of inhibition of the antigen-antibody reaction with heterologous hemoglobins appeared to be related to the structural si...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484406
更新日期：1975-12-01 00:00:00
abstract：:Alcohol dehydrogenase class IV (ADH4) participates in retinol metabolism and is expressed primarily in ocular, digestive, and reproductive tissues of the mouse. A naturally occurring genetic variant in C57BL/6J mice results in a faster migrating ADH4 enzyme during electrophoresis when compared to other non-C57BJ/6J st...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1010278631535
更新日期：2001-08-01 00:00:00
abstract：:There are three major groups of domestic cattle in East and Southeast Asia: European cattle, Zebu cattle, and Bali cattle. Ten restriction enzymes were used to analyze restriction site variants in the mitochondrial DNA (mtDNA) in 178 individuals belonging to these three groups of cattle. The results indicate that each...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554558
更新日期：1995-02-01 00:00:00
abstract：:Different homozygous lines of similar genotype with respect to G6pd and 6Pgd were shown to have different enzyme activities for G6PD and 6PGD. Crosses between high and low lines suggested that there were modifying genes present on the autosomes, while others were probably located on the X chromosome. Allelic variation...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484587
更新日期：1980-08-01 00:00:00
abstract：:Single-chain variable fragment (scFv) is one of the most common antibody forms. This report describes the expression of the scFv gene as a soluble protein in Origami DE3 cytoplasm. The purified scFv recognized the epidermal growth factor receptor (EGFRvIII) on the surface of MCF-7 cells. The scFv protein was purified ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-012-9506-4
更新日期：2012-08-01 00:00:00
abstract：:Fifty-six sequences encoding the pina protein were characterized from three species or subspecies of einkorn wheat. These sequences contained 1,595 nucleotides, including 1,270 conserved sites, 21 single nucleotide polymorphisms (SNPs), and 16 indels. The average frequency of SNPs and indels was one out of 76.1 and 99...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-009-9239-1
更新日期：2009-06-01 00:00:00
abstract：:Liver homogenate-supernatants from most Japanese exhibit an "atypical" pH optimum for ethanol oxidation at pH 8.8 instead of 10.5, the "typical" pH-activity optimum. It has been proposed that atypical livers contain alcohol dehydrogenase isozymes with beta 2 subunits while typical livers contain isozymes with beta 1 s...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499296
更新日期：1984-02-01 00:00:00
abstract：:This work examines the molecular evolution of a brain-expressed X-linked gene family in the mammalian genomes of human, chimp, macaque, mouse, rat, dog, and cow. The gene structures are well conserved across family members and among the mammals in that all five members have three exons with the first two exons untrans...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-008-9148-8
更新日期：2008-06-01 00:00:00
abstract：:The genetic divergence, population genetic structure, and possible speciation of the Korean firefly, Pyrocoelia rufa, were investigated on the midsouthern Korean mainland, coastal islets, a remote offshore island, Jedu-do, and Tsushima Island in Japan. Analysis of DNA sequences from the mitochondrial COI protein-codin...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/b:bigi.0000007777.87407.1b
更新日期：2003-12-01 00:00:00
abstract：:Ade-H and ade-I are two auxotrophic mutants of Chinese hamster ovary (CHO-K1) cells which specifically require adenine as the purine source to grow. The enzymatic defects of these mutants were examined in cell-free extracts. It was found that ade-H did not have any detectable adenylosuccinate synthetase activity and a...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484561
更新日期：1977-02-01 00:00:00
abstract：:Maternal embryo leucine zipper kinase (MELK) has a higher expression level in a variety of cancers and involved in progression of colorectal cancer. The MELK expression levels in colorectal cancer tissues and cells were detected by RT-qPCR. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwe...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09974-x
更新日期：2020-10-01 00:00:00
abstract：:Nineteen inbred strains of the laboratory rat (Rattus norvegicus) were investigated for genetic differences by starch and agarose gel electrophoretic techniques. For six out of 21 loci, interstrain differences were observed: Tf, 6PGD, Es-1, Es-4, Es-5, and Svp. The variants are briefly described and the allelic distri...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484206
更新日期：1978-06-01 00:00:00
abstract：:A new polymorphism, Pc, has been identified in human saliva. Two proteins, Pc 1 and Pc 2, are determined by alleles Pc1 and Pc2, respectively, which show autosomal codominant inheritance. No null phenotype has been encountered in 225 randomly collected salivas. The frequencies of the two alleles differ in the Black an...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499111
更新日期：1985-02-01 00:00:00
abstract：:Restriction endonuclease fragment length variations (RFLVs) were detected by using a rat cDNA probe for the bilirubin UDP-glucuronosyltransferase (UDPGT) gene between two mouse strains, 129/Sv and MOL-MIT. RFLVs of the gene were found by EcoRI and PvuII digestions. From linkage analyses of the three-point cross test u...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00569325
更新日期：1992-08-01 00:00:00
abstract：:Using megagametophyte (maternal haploid) and embryo (diploid) tissues of incense-cedar seeds, the expression of one of three malate dehydrogenase (MDH) loci was found to be influenced by a second, unlinked, modifier locus. Whereas alleles of the affected structural locus are codominant, the modifier alleles show domin...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484435
更新日期：1983-06-01 00:00:00
abstract：:The allele, genotype, and haplotype frequencies among 837 Polish Holstein-Friesian cattle were determined at two regulatory indel polymorphisms of the PRNP gene. Allele frequencies at the 23 bp indel promoter polymorphism were 0.622 (del) and 0.378 (ins), with 0.613 and 0.387 in sires and 0.633 and 0.366 in dams. Alle...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9408-2
更新日期：2011-06-01 00:00:00
abstract：:Oral cancer is a multifactorial disease process and involves complex interactions between gene to gene and gene to environmental factors. Interleukin 8 (IL-8), a pro-inflammatory cytokine, having angiogenic activity with elevated expression in tumor cells, is reported to play an essential role in oral cancer developme...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-015-9704-y
更新日期：2016-02-01 00:00:00
abstract：:The ribosomal RNA cistrons of three species of trout: Salvelinus namaycush (lake trout), Salvelinus fontinalis (brook trout), and Salmo gairdneri (rainbow trout) were examined by Southern blot analysis of genomic DNA. The repeat length of the cistron of S. namaycush is 26 kb. A repeat-length polymorphism was observed ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499943
更新日期：1985-12-01 00:00:00
abstract：:Patterns of lactate dehydrogenase (LDH) isozymes of tissues from various mouse strains were examined. An interstrain polymorphism for LDH isozymes of liver was established. One phenotype (CBA/Lac and AKR/J mice) yielded a five-banded LDH pattern, another phenotype (DBA/1J, DBA/2J, C57BL/6J and C3H/He) showed a three-b...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00500133
更新日期：1980-10-01 00:00:00
abstract：:Techniques for performing numerous enzyme kinetic assays with minimum time and effort would be valuable to studies of the evolutionary genetics of metabolic control and the quantitative genetics of determinants of kinetic parameters. Microtiter plate readers have been used for a variety of repetitious analytical techn...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554162
更新日期：1989-06-01 00:00:00
abstract：:A mouse mutant with pyruvate kinase (PK) hyperactivity has been found in offspring of 1-ethyl-1-nitrosourea (ENU)-treated male mice. The activity alteration was detected in the blood and could also be found in the liver but not in the muscle, kidney, heart, spleen, lung, or brain. Heterozygous mice have erythrocyte PK...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499635
更新日期：1984-12-01 00:00:00
abstract：:The structural gene locus specifying subunits of cytoplasmic isoenzymes of phosphoglucose isomerase (PGI) is present in duplicate in many diploid species of Clarkia (Onagraceae), a genus of annual plants native to California. We studied the kinetic properties and molecular weights of a large number of genetically defi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00486145
更新日期：1981-02-01 00:00:00
abstract：:Arylsulfatase A (ASA) activity was studied from five blood fractions, leukocytes plus platelets (LKPL), leukocytes (LK), neutrophils (N), lymphocytes (LYM), and platelets (PL). No significant difference was found among the mean ASA specific activity values for the fractions. Electrophoretic examination revealed four d...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484068
更新日期：1982-10-01 00:00:00
abstract：:An Italian organophosphate-resistant strain of Culex pipiens (Lucca) was found to be polymorphic for elevated and nonelevated esterases. Selection for high esterase activity produced a strain homozygous for elevated esterases A2 and B2. Selection for low activity produced a strain homozygous for nonelevated esterases,...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426878
更新日期：1993-12-01 00:00:00
abstract：:Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patter...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9607-8
更新日期：2013-10-01 00:00:00
abstract：:To elucidate the phylogeny of the genus Paramesotriton (Caudata: Salamandridae), we investigated three mitochondrial DNA gene fragments (1207 bp in total) of cytochrome b, ND2, and ND4 for its six recognized species. The phylogenetic relationships within Paramesotriton were reconstructed by maximum parsimony (MP) and ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/b:bigi.0000026630.78770.fb
更新日期：2004-06-01 00:00:00
abstract：:Random amplified polymorphic DNA was used to assess the level of genetic diversity and genetic structure of Stipa krylovii (Gramineae), an important dominant species in the northern grasslands of China. Genetic diversity was low within S. krylovii populations, and diversity at the population level was associated with ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-006-9054-x
更新日期：2006-12-01 00:00:00
