Biochemical properties of duplicated isozymes of phosphoglucose isomerase in the plant Clarkia xantiana.

Abstract:

:The structural gene locus specifying subunits of cytoplasmic isoenzymes of phosphoglucose isomerase (PGI) is present in duplicate in many diploid species of Clarkia (Onagraceae), a genus of annual plants native to California. We studied the kinetic properties and molecular weights of a large number of genetically defined and highly purified PGIs in C. xantiana, a number of species with the duplication, as a means of examining the biochemical consequences of the evolution of a new gene locus. This species is primarily outcrossing, but also includes several previously described predominantly self-pollinating populations. Both cytoplasmic PGI loci in the outcrossing populations are polymorphic and their enzyme products are readily separated by electrophoresis. The PGIs from the outcrossing populations were rated by electrophoresis. The PGIs from the outcrossing populations were generally closely similar in molecular weight, pH optimum, heat sensitivity, energy activation, and apparent Km (fructose-6-phosphate). The PGI loci in the selfing populations are monomorphic and specify enzymes having identical electrophoretic mobilities to those coded by the most frequent alleles of the outcrosser. The PGI isozymes in the selfers differed fivefold in Km, suggesting that they have a very different catalytic effectiveness. The high Km of the PGI-3A' isozyme (1.1mM) was anomalous among the PGIs examined and would likely be disadvantageous in a species which lacked other more normally functioning PGIs. But in the cytoplasm of the selfing plants, it is present with other PGIs that have low Km values. The PGI-3A' enzyme is a good candidate for a gene product coded by a "forbidden" mutation that could not have been established in the absence of the duplication. The rationale for this suggestion is described and it is also pointed out that the divergence of duplicated genes is influenced by many factors such as the breeding system and other population factors as well as the effect of particular mutations.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Gottlieb LD,Greve LC

doi

10.1007/BF00486145

subject

Has Abstract

pub_date

1981-02-01 00:00:00

pages

155-72

issue

1-2

eissn

0006-2928

issn

1573-4927

journal_volume

19

pub_type

杂志文章
  • Biochemical markers in rats: linkage relationships of aconitase (Acon-1), aldehyde dehydrogenases (Ahd-2 and Ahd-c), alkaline phosphatase (Akp-1), and hydroxyacid oxidase (Hao-1).

    abstract::We have examined the linkage relationships between five biochemical markers, Acon-1, Ahd-2, Ahd-c, Akp-1, and Hao-1, and 19 other genetic loci in five breeding combinations. The genetic locus that codes for a recently described aldehyde dehydrogenase in the liver (Ahd-c) has been assigned to linkage group X (LG X). Hy...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00502790

    authors: Cramer DV,Mowery PA,Adams M

    更新日期:1986-04-01 00:00:00

  • Assignment of a gene for tryptophanyl-transfer ribonucleic acid synthetase (E.C. 6.1.1.2) to human chromosome 14.

    abstract::We describe a simple method for locating tryptophanyl-tRNA synthetase (E.C. 6.1.1.2) on cellulose acetate gels (Cellogel) following electrophoresis. Employing electrophoretic conditions which result in the separation of mouse and human tryptophanyl-tRNA synthetase, we have analyzed extracts of a number of independentl...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00484877

    authors: Denney RM,Craig IW

    更新日期:1976-02-01 00:00:00

  • A study of genetic structure of Stephania yunnanensis (Menispermaceae) by DALP.

    abstract::Genetic diversity and genetic structure within and among ten populations of Stephania yunnanensis H. S. Lo and three populations of S. epigaea H. S. Lo from Yunnan province were evaluated by direct amplification of length polymorphism (DALP) markers. Five primer groups were screened, and a total of 287 DNA fragments w...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-008-9146-x

    authors: Ma YS,Yu H,Li YY,Yan H,Cheng X

    更新日期:2008-04-01 00:00:00

  • A new pyruvate kinase mutation with hyperactivity in the mouse.

    abstract::A mouse mutant with pyruvate kinase (PK) hyperactivity has been found in offspring of 1-ethyl-1-nitrosourea (ENU)-treated male mice. The activity alteration was detected in the blood and could also be found in the liver but not in the muscle, kidney, heart, spleen, lung, or brain. Heterozygous mice have erythrocyte PK...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00499635

    authors: Charles DJ,Pretsch W

    更新日期:1984-12-01 00:00:00

  • Qualitative changes in carboxylesterase-2 phenotypes of tetraploid loaches (Misgurnus fossilis L.).

    abstract::We have shown in our previous work that in the tetraploid loach Misgurnus fossilis carboxylesterase-2 (E-2) phenotypes of embryos and early larvae are controlled by the alleles of the Est-2 locus. In the present work it has been established that the somatic tissues of many adult fish homozygous for one of the Est-2 al...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00484449

    authors: Ivanenkov VV

    更新日期:1983-06-01 00:00:00

  • Polymorphisms of DNA repair genes: ERCC1 G19007A and ERCC2/XPD C22541A in a northeastern Chinese population.

    abstract::DNA repair systems are responsible for maintaining the integrity of the genome and have a critical role in protecting against mutations that can lead to cancer. DNA repair gene products of ERCC1 and ERCC2/XPD are involved in the nucleotide excision repair pathway. The allele frequencies of the polymorphisms ERCC1 G190...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-005-8170-3

    authors: Yin J,Li J,Vogel U,Wang H

    更新日期:2005-10-01 00:00:00

  • Down-Regulation of SIRT1 Expression by mir-23b Contributes to Lipid Accumulation in HepG2 Cells.

    abstract::Non-alcoholic fatty liver disease is one of the main causes of chronic liver disease and therefore is currently considered a major public health problem. Sirtuin 1 (SIRT1) is an NAD-dependent deacetylase enzyme that contributes in the regulation of metabolic processes and protects against lipid accumulation in hepatoc...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-019-09905-5

    authors: Borji M,Nourbakhsh M,Shafiee SM,Owji AA,Abdolvahabi Z,Hesari Z,Ilbeigi D,Seiri P,Yousefi Z

    更新日期:2019-08-01 00:00:00

  • Genetic control of chalcone isomerase activity in flowers of Dianthus caryophyllus.

    abstract::In flowers of Dianthus caryophyllus (carnation), the gene I is concerned with a discrete step in flavonoid biosynthesis, Genotypes with recessive (ii) alleles produce yellow flowers, which contain the chalcone isosalipurposide (naringenin-chalcone-2'-glucoside) as the major petal pigment, but in genotypes with wild-ty...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00484399

    authors: Forkmann G,Dangelmayr B

    更新日期:1980-06-01 00:00:00

  • Biochemical evaluation of triploid progenies of diploid × tetraploid breeding populations of Camellia for genotypes rich in catechin and caffeine.

    abstract::To verify the quality of triploid varieties of Camellia tea species at the secondary metabolite level, we tested caffeine and catechin profiles of 97 F(1) segregating progenies in two breeding populations with a common tetraploid parent and diploid parents of two geographic and varietal origins. Catechin and caffeine ...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-013-9569-x

    authors: Das SK,Sabhapondit S,Ahmed G,Das S

    更新日期:2013-06-01 00:00:00

  • Expression of activin receptor-like kinase 7 in adipose tissues.

    abstract::The tissue distribution of activin receptor-like kinase 7 (Alk7) expression, the signaling ability of Alk7 variants, and Alk7 expression in response to β3-adrenergic receptor activation were examined. Expression levels of Alk7 varied greatly among tissues but were highest in white adipose tissue and brown adipose tiss...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-012-9555-8

    authors: Murakami M,Shirai M,Ooishi R,Tsuburaya A,Asai K,Hashimoto O,Ogawa K,Nishino Y,Funaba M

    更新日期:2013-04-01 00:00:00

  • Peptidase B polymorphism in cattle erythrocytes.

    abstract::Starch gel electrophoresis and histochemical staining with L-leucylglycyl-glycine revealed genetic polymorphism in peptidase B in cattle erythrocytes. Genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, PepB1 and PepB2. A...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:

    authors: Del Lama SN,Del Lama MA,Mestriner MA,Mortari N

    更新日期:1992-06-01 00:00:00

  • Description of a genetic polymorphism of a human proline-rich salivary protein, Pc, and its relationship to other proteins in the salivary protein complex (SPC).

    abstract::A new polymorphism, Pc, has been identified in human saliva. Two proteins, Pc 1 and Pc 2, are determined by alleles Pc1 and Pc2, respectively, which show autosomal codominant inheritance. No null phenotype has been encountered in 225 randomly collected salivas. The frequencies of the two alleles differ in the Black an...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00499111

    authors: Karn RC,Goodman PA,Yu PL

    更新日期:1985-02-01 00:00:00

  • Assignment of 6-phosphogluconate dehydrogenase and malate dehydrogenase to chromosome 3 of Anopheles stephensi.

    abstract::Genetics and linkage analysis of 6-phosphogluconate dehydrogenase (6-PGD) and malate dehydrogenase (MDH) have been investigated in Anopheles stephensi. Both these markers were found to be autosomal and linked and have been assigned to linkage group III. Two mutant markers, Black larva (Bl) and golden-yellow larva (gy)...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF01037589

    authors: Adak T,Subbarao SK,Sharma VP,Rao SR

    更新日期:1992-10-01 00:00:00

  • Genetic, ontogenetic, and tissue-specific variation of dipeptidases in Drosophila melanogaster.

    abstract::Three dipeptidases in Drosophila melanogaster are under independent genetic control and their structural genes have been localized, Dip-A to 2R and Dip-B and Dip-C to 3R (Voelker and Langley, 1978; Ohnishi and Voelker, 1981). These enzymes were characterized with respect to their substrate specificities, genetic varia...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00484692

    authors: Laurie-Ahlberg CC

    更新日期:1982-06-01 00:00:00

  • Gene duplication in salmonid fishes: evidence for duplicated but catalytically equivalent A4 lactate dehydrogenases.

    abstract::Skeletal muscle tissues from many species of salmonid fish are known to exhibit a set of three of five isozymes for A4-type lactate dehydrogenase (L-lactate: NAD oxidoreductase, E.C. 1.1.1.27), but the genetic basis for this isozyme system has not previously been assessed. This isozyme system was purified to homogenei...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00484099

    authors: Lim ST,Bailey GS

    更新日期:1977-08-01 00:00:00

  • A new locus regulating the expression of the Ldh-2 gene in mouse liver.

    abstract::Patterns of lactate dehydrogenase (LDH) isozymes of tissues from various mouse strains were examined. An interstrain polymorphism for LDH isozymes of liver was established. One phenotype (CBA/Lac and AKR/J mice) yielded a five-banded LDH pattern, another phenotype (DBA/1J, DBA/2J, C57BL/6J and C3H/He) showed a three-b...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00500133

    authors: Khlebodarova TM,Serov OL

    更新日期:1980-10-01 00:00:00

  • Human platelet phenol sulfotransferase: familial variation in thermal stability of the TS form.

    abstract::Phenol sulfotransferase (PST) catalyzes the sulfate conjugation of catechol and phenolic drugs and xenobiotic compounds. Platelets and other tissues contain at least two forms of PST, forms that have been designated the "TL" and the "TS" forms. We measured the thermal stability of platelet TS PST in blood samples from...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00499627

    authors: Van Loon J,Weinshilboum RM

    更新日期:1984-12-01 00:00:00

  • Genetic polymorphism of apolipoprotein C polypeptide in native Asian sheep.

    abstract::The frequency distributions of apolipoprotein C alleles in native Asian sheep (Bengal, Kagi, Lampuchhre, Vietnamese, Myanmar, Baruwal, Bhyanglung, Khalkhas, and Kuwait) were determined using a one-dimensional polyacrylamide gel isoelectric-focusing immunoblotting technique. Among these Asian sheep, the genetic locus c...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1023/a:1020781629510

    authors: Tsunoda K,Watanabe S,Sato J,Sato K

    更新日期:2002-12-01 00:00:00

  • Assignment of six enzyme loci to multipoint linkage groups in fishes of the genus Poeciliopsis (Poeciliidae): designation of linkage groups III-V.

    abstract::Three new linkage groups of enzyme loci are described using Poeciliopsis monacha x P. viriosa-derived interspecific backcross hybrids. Comparison to known linkage groups of the confamilial genus Xiphophorus shows homology between Xiphophorus linkage group I and Poeciliopsis linkage group III, Xiphophorus linkage group...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00554823

    authors: Morizot DC,Schultz RJ,Wells RS

    更新日期:1990-02-01 00:00:00

  • The Impact of Maternal-Fetal Genetic Conflict Situations on the Pathogenesis of Preeclampsia.

    abstract::Preeclampsia leads to maternal and perinatal morbidity and mortality. The literature in English was reviewed to summarize recent advances in understanding the global gene expression changes in the pathogenesis of preeclampsia. We identified at least eight consistently enriched categories, relating to pregnancy mainten...

    journal_title:Biochemical genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s10528-015-9684-y

    authors: Kobayashi H

    更新日期:2015-10-01 00:00:00

  • The telomere-binding protein Taz1p as a target for modification by a SUMO-1 homologue in fission yeast.

    abstract::In fission yeast (Schizosaccharomyces pombe) the homologue of the mammalian SUMO-1 ubiquitin-like modifier is encoded by the pmt3 gene. A two-hybrid screen using the telomere-binding protein Taz1p as bait identified Pmt3p as an interacting factor. In vitro experiments using purified components of the fission yeast Pmt...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-005-1503-4

    authors: Spink K,Ho JC,Tanaka K,Watts FZ,Chambers A

    更新日期:2005-04-01 00:00:00

  • Genetic and cytogenetic studies of malic enzyme in Drosophila melanogaster.

    abstract::The genetic and cytogenetic locations of the structural gene (Men) for malic enzyme have been determined. Men maps genetically between kar and ry at 51.73 +/- 0.02. Cytogenetically, Men probably lies in the proximal edge of 87D1,2, based on the results of mapping utilizing a number of deficiencies with breakpoints in ...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00484624

    authors: Voelker RA,Ohnishi S,Langley CH,Gausz J,Gyurkovics H

    更新日期:1981-06-01 00:00:00

  • Expression and characterization of a functional single-chain variable fragment (scFv) protein recognizing MCF7 breast cancer cells in E. coli cytoplasm.

    abstract::Single-chain variable fragment (scFv) is one of the most common antibody forms. This report describes the expression of the scFv gene as a soluble protein in Origami DE3 cytoplasm. The purified scFv recognized the epidermal growth factor receptor (EGFRvIII) on the surface of MCF-7 cells. The scFv protein was purified ...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-012-9506-4

    authors: Mahgoub IO

    更新日期:2012-08-01 00:00:00

  • Evidence for introgressive hybridization of captive markhor (Capra falconeri) with domestic goat: cautions for reintroduction.

    abstract::Markhors (Capra falconeri) are among the most endangered mammal species, and several conservation measures, including ex situ breeding, are implemented to prevent their extinction. We studied sequence diversity and differentiation of the first hypervariable segment of the mitochondrial DNA control region among C. f. h...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-008-9145-y

    authors: Hammer SE,Schwammer HM,Suchentrunk F

    更新日期:2008-04-01 00:00:00

  • Composition and genetic variability of heparin-sepharose CL-6B protein fractions obtained from the solubilized proteins of mouse organs.

    abstract::The solubilized proteins of liver and brain from mice of two inbred strains (C57BL/6J and DBA/2J) and their hybrids were subfractionated by heparin Sepharose (H-S) CL-6B affinity chromatography. The H-S binding and nonbinding proteins were separated by two-dimensional electrophoresis. The protein patterns obtained wer...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00554529

    authors: Jungblut P,Klose J

    更新日期:1986-12-01 00:00:00

  • A cluster of at least three esterase genes in Lucilia cuprina includes malathion carboxylesterase and two other esterases implicated in resistance to organophosphates.

    abstract::Three distinct malathion carboxylesterase (MCE) phenotypes have been identified among strains of Lucilia cuprina. The high-activity phenotype shows 1.6- and 33-fold more MCE specific activity than the intermediate- and low-activity phenotypes, respectively. Flies with high MCE activity are 1000-fold more resistant to ...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00566064

    authors: Smyth KA,Russell RJ,Oakeshott JG

    更新日期:1994-12-01 00:00:00

  • Polymorphism of blood plasma proteins in the Anser and Branta genera.

    abstract::An electrophoretical analysis of blood plasma proteins of eight Anser and two Branta species was performed. Ten polymorphic proteins in blood plasma pattern were distinguished and described: four prealbumin proteins, albumin, three postalbumin proteins, transferrin, and a single posttransferrin protein. Genus-specific...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00557950

    authors: Kuznetsov SB

    更新日期:1995-04-01 00:00:00

  • Populationwide investigation of two indel polymorphisms at the prion protein gene in Polish Holstein-Friesian cattle.

    abstract::The allele, genotype, and haplotype frequencies among 837 Polish Holstein-Friesian cattle were determined at two regulatory indel polymorphisms of the PRNP gene. Allele frequencies at the 23 bp indel promoter polymorphism were 0.622 (del) and 0.378 (ins), with 0.613 and 0.387 in sires and 0.633 and 0.366 in dams. Alle...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/s10528-010-9408-2

    authors: Czarnik U,Strychalski J,Zabolewicz T,Pareek CS

    更新日期:2011-06-01 00:00:00

  • Linkage analyses and biochemical genetics of sorbitol dehydrogenase-1 (Sdh-1) in the mouse.

    abstract::Electrophoretic variation of sorbitol dehydrogenase has been found in Mus musculus; the SDH-1 A phenotype migrates further toward the cathode under the conditions used than the SDH-1B phenotype. Mice with the SDH-1B phenotype have enzyme activity reduced to 25% of that found in mice with the SDH-1A phenotype, but no d...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1007/BF00498928

    authors: Andrews SJ,Peters J

    更新日期:1983-08-01 00:00:00

  • Inheritance of allozyme loci in Bombina: second linkage group established.

    abstract::Segregation and linkage relationship of nine allozyme loci, which are fixed for alternative alleles in the European fire-bellied toads, Bombinal bombina and B. variegata, were studied using artificial F1 hybrids to obtain backcross and F2 progeny. Alleles coding for electromorphs at nine loci (Ldh-1, Mdh-1, Idh-1, Ck,...

    journal_title:Biochemical genetics

    pub_type: 杂志文章

    doi:10.1023/a:1002032005853

    authors: Hofman S,Szymura JM

    更新日期:2000-08-01 00:00:00