Abstract：
:Maternal embryo leucine zipper kinase (MELK) has a higher expression level in a variety of cancers and involved in progression of colorectal cancer. The MELK expression levels in colorectal cancer tissues and cells were detected by RT-qPCR. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwell assays were used to examine the effect of the MELK konckdown on the proliferation, migration and invasion of colorectal cancer cells. Western blot analysis was used to detect the protein level of MELK and the downstream signaling pathways related proteins. Our findings indicated that MELK expression in colorectal cancer tissues was significantly higher than that in para-carcinoma tissues. Knockdown of MELK with shRNA had strong inhibition effects on the proliferation, migration and invasion of colorectal cancer cells. MELK knockdown could also decrease the phosphorylation level of AKT through FAK/Src pathway. Our results indicated downregulation of MELK retarded the progression of CRC by inhibition of the phosphorylation level of AKT through inactivating FAK/Src pathways. Therefore, MELK has the potential to be explored as a new therapeutic target and knockdown can be used as a potential treatment strategy for colorectal cancer.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Liu G,Zhan W,Guo W,Hu F,Qin J,Li R,Liao Xdoi
10.1007/s10528-020-09974-xsubject
Has Abstractpub_date
2020-10-01 00:00:00pages
771-782issue
5eissn
0006-2928issn
1573-4927pii
10.1007/s10528-020-09974-xjournal_volume
58pub_type
杂志文章abstract：:Male and female wild Norway rats (Rattus norvegicus Erxleben) and male and female albino outbred rats (Ipf:RIZ) were crossbred. These animals were the control, noninbred group (0% inbred). By systematic full-sib mating, two experimental groups (50 and 91% of inbred) were raised. Half of each group (both males and fema...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00569326
更新日期：1992-08-01 00:00:00
abstract：:Genetic variations affecting the course of depressive symptoms in patients with coronary artery disease (CAD) have not yet been well studied. Therefore, we set out to investigate whether distinct haplotypes of the two insertion/deletion polymorphisms in the serotonin-transporter-linked polymorphic region (5-HTTLPR) an...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09967-w
更新日期：2020-08-01 00:00:00
abstract：:We extracted the yellow melanin (phaeomelanin), black melanin (eumelanin), and mixed type of melanin from dorsal hair of dominant yellow (Ay/a), non-agouti (a/a), and agouti (A/A) mice, respectively. Spectrophotometric and fluorescence spectrophotometric analysis demonstrated that the yellow melanin was qualitatively ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484724
更新日期：1978-08-01 00:00:00
abstract：:Active-site tryptic peptides were isolated from three genetic types of human serum cholinesterase. The active-site peptide was identified by labeling the active-site serine with [3H]diisopropylfluorophosphate. Peptides were purified by high-performance liquid chromatography. Amino acid composition and sequence analysi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499101
更新日期：1986-06-01 00:00:00
abstract：:A plausible approach to evaluate the inhibitory action of antifungals is through the investigation of the fungal resistance to these drugs. We describe here the molecular cloning and initial characterization of the A. nidulans lipA gene, where mutation (lipA1) conferred resistance to undecanoic acid, the most fungitox...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-008-9170-x
更新日期：2008-10-01 00:00:00
abstract：:In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene appears to play a role in nitrogen catabolite repression. Using the N. crassa nmr gene as a probe, homologous sequences were ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:The concentration of mouse kidney histidine decarboxylase (HDC) is modulated by estrogen, testosterone, and thyroxine in a tissue-specific manner. Variation in HDC levels between strains of mice can be used to investigate the genetic regulation of enzyme structure, tissue specific expression, and induction and repress...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485850
更新日期：1984-08-01 00:00:00
abstract：:Among the European fauna, the Sardinian hare (Lepus sp.) is peculiar in that it differs from all other hares inhabiting the continent. Here, we report on the variation of a 461 bp sequence of hypervariable domain 1 of the mitochondrial control region, examined in 42 hares collected throughout Sardinia and compared to ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-007-9076-z
更新日期：2007-04-01 00:00:00
abstract：:We report a new enzyme xylose dehydrogenase, the structural locus for which is on chromosome 7 of the mouse, closely linked to Tam-1. Three alleles have been detected in both laboratory strains and wild populations. Two of these determine proteins differing in electrophoretic mobility and the third is a "null." This e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483970
更新日期：1982-08-01 00:00:00
abstract：:The ratio of cellular RNA and protein content is about 1:1 between phylogenetically diploid and tetraploid species of the teleost family Cyprinidae, but is roughly in proportion to ploidy in species of the teleost order Isospondyli. Enzyme activities do not unequivocally comply with this scheme. These findings are dis...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485129
更新日期：1976-12-01 00:00:00
abstract：:The genetic differentiation among the populations of the European abalone Haliotis tuberculata was investigated using different markers to better understand the evolutionary history and exchanges between populations. Three markers were used: mitochondrial cytochrome oxidase I (COI), the sperm lysin nuclear gene, and e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-016-9778-1
更新日期：2017-04-01 00:00:00
abstract：:Upland rice landraces from different villages of Mizoram, Northeast India were analyzed for seed morphology, amylose content, aromatic characteristic, seed storage protein profiling and genetic diversity. Results revealed variation in grain length, width, weight and shape. Protein profiling showed polypeptide bands ra...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-09901-1
更新日期：2019-06-01 00:00:00
abstract：:Oral cavity cancer belongs to head-and-neck squamous cell carcinoma group. The purpose of the study was to assess the levels of certain proteins in a tumour and surgical margin in a group of patients with oral cavity cancer. The levels of DAPK1, MGMT, CDH1, SFRP1, SFRP2, RORA, TIMP3, p16, APC and RASSF1 proteins were ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-017-9799-4
更新日期：2017-08-01 00:00:00
abstract：:The inducibility of alcohol dehydrogenase (ADH) has been recognized in different systems including maize, Drosophila, and mice. Our earlier results showed strain-specific ADH responses to chronic ethanol administration relative to matched littermate controls in mice. For this study we used two strains which showed "in...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485847
更新日期：1984-08-01 00:00:00
abstract：:When the 4-bp Dam recognition sequence was placed between two d(GA)7 tracts, it became severely undermethylated in JM101 Escherichia coli cells compared to other Dam sequences in the same plasmid DNA. This site specific undermethylation was also detected on supercoiled molecules in vitro. Mutational analysis indicated...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02407017
更新日期：1996-06-01 00:00:00
abstract：:A bioautographic procedure has been developed for the visualization of the isozymes of dihydrofolate reductase (DHFR, E.C. 1.5.1.3). In addition to detecting electrophoretically separated enzymes, bioautography was utilized to visualize DHFR after isoelectric focusing on polyacrylamide gels. Both zone electrophoresis ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504369
更新日期：1980-02-01 00:00:00
abstract：:An electrophoretical analysis of blood plasma proteins of eight Anser and two Branta species was performed. Ten polymorphic proteins in blood plasma pattern were distinguished and described: four prealbumin proteins, albumin, three postalbumin proteins, transferrin, and a single posttransferrin protein. Genus-specific...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00557950
更新日期：1995-04-01 00:00:00
abstract：:Glutamate-oxaloacetate transaminase (GOT; EC 2.6.1.1) occurs as two electrophoretically distinguishable isozymes in the copepod Tigriopus californicus. The slower-migrating form, referred to as GOT2 , is shown to be associated with the mitochondrial cell fraction. GOT2 phenotypes are inherited in typical Mendelian fas...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484232
更新日期：1984-04-01 00:00:00
abstract：:A mouse mutant with pyruvate kinase (PK) hyperactivity has been found in offspring of 1-ethyl-1-nitrosourea (ENU)-treated male mice. The activity alteration was detected in the blood and could also be found in the liver but not in the muscle, kidney, heart, spleen, lung, or brain. Heterozygous mice have erythrocyte PK...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499635
更新日期：1984-12-01 00:00:00
abstract：:This haplotype-based case-control study investigated whether the aldosterone synthase gene (CYP11B2) might be implicated in the pathogenesis of essential hypertension in Yi (226 individuals) and Hani (296 individuals) minorities of China. Four tag SNPs (rs4536, rs4545, rs3097, and rs3802230) and the K173R polymorphism...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9393-5
更新日期：2011-02-01 00:00:00
abstract：:Phosphoglucomutase (PGM) of adult stage in Drosophilia melanogaster has been characterized by gel filtration, ion-exchange chromatography, and isoelectric focusing. The two common electrophoretic variants, PGMA and PGMB, differ with respect to their kinetic and stability parameters. PGMA is more thermostable than PGMB...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504306
更新日期：1979-10-01 00:00:00
abstract：:Genetic diversity and differentiation of nine populations of Ginkgo biloba L. (Ginkgoaceae)from China were evaluated using RAPD. Of 47 clear and repeatable RAPD bands, 46 were polymorphic (overall polymorphism = 97.9%). A ranged from 1.57 to 1.83 with a mean of 1.75. Mean He was 0.3159 (0.2429-0.3603). The Shannon ind...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/b:bigi.0000034431.15308.57
更新日期：2004-08-01 00:00:00
abstract：:The frequency distributions of apolipoprotein C alleles in native Asian sheep (Bengal, Kagi, Lampuchhre, Vietnamese, Myanmar, Baruwal, Bhyanglung, Khalkhas, and Kuwait) were determined using a one-dimensional polyacrylamide gel isoelectric-focusing immunoblotting technique. Among these Asian sheep, the genetic locus c...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1020781629510
更新日期：2002-12-01 00:00:00
abstract：:Apodemus peninsulae is a field mouse that inhabits the broad-leafed forests of temperate Eurasia. We examined the mitochondrial cytochrome b gene in 57 in dividuals of A. peninsulae from northeastern Asia, including Siberia, Primorye, Magadan region, Sakhalin, Hokkaido, and the Korean Peninsula. The genealogy of the m...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1015841424598
更新日期：2002-06-01 00:00:00
abstract：:Genetic variation of a codominantly inherited pancreas protease, designated CTRA-1, was discovered in the house mouse by isoelectric focusing in polyacrylamide gels. Phenotype CTRA-1A was found in MOLH/Fre and in the majority of common laboratory mouse strains. Phenotype CTRA-1B was found in PWD/Ph. It was characteriz...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426870
更新日期：1991-12-01 00:00:00
abstract：:The aryl hydrocarbon hydroxylase (Ah) locus that controls the induction of chemical carcinogen-metabolizing enzymes in mice has been found to be linked to a new restriction-fragment length polymorphism (RFLP). Only C57BL/6 and closely related inbred strains displayed a 7.6-kb HindIII restriction fragment, while all ot...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554549
更新日期：1987-06-01 00:00:00
abstract：:In corn seedlings (Zea mays L.) homozygous for the mutation gl5, the surface waxes are characteristically altered. In this mutant the main wax constituents (83.5%) are aldehydes while in the normal waxes alcohols predominate (62.7%). Moreover, in the normal waxes aldehydes and alcohols are made up mainly of the C32 te...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483751
更新日期：1978-10-01 00:00:00
abstract：:It is shown that the gene controlling the synthesis of the organ-specific S-esterase of Drosophila virilis ejaculatory bulbs is located on the second chromosome (at approximate position 192.1 +/- map units). The cells of the genital imaginal disks are determined for the synthesis of S-esterase 10-12 hr after the secon...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484728
更新日期：1978-08-01 00:00:00
abstract：:ZFY-like genes have been observed in a variety of vertebrate species. Although originally implicated as the primary testis-determining gene in humans and other placental mammals, more recent evidence indicates a role(s) outside that of testis determination. In this study, DNA from five species of fish, Carasius auratu...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:The primary structures of the alpha globins from CE/J, DBA/2J, and a stock of Potter's mice were determined to identify the amino acid substitutions associated with the unique isoelectric focusing patterns of these hemoglobins. In addition, the primary structures of the alpha globins from MOL III and PERU mice were st...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484946
更新日期：1982-02-01 00:00:00
