Bioautographic visualization of dihydrofolate reductase in enzyme overproducing BHK mutants.

abstract：:In flowers of Dianthus caryophyllus (carnation), the gene I is concerned with a discrete step in flavonoid biosynthesis, Genotypes with recessive (ii) alleles produce yellow flowers, which contain the chalcone isosalipurposide (naringenin-chalcone-2'-glucoside) as the major petal pigment, but in genotypes with wild-ty...

journal_title：Biochemical genetics

authors： Forkmann G,Dangelmayr B

更新日期：1980-06-01 00:00:00

abstract：:Genetic variation and differentiation of 12 populations of Picea jezoensis from the Russian Far East were studied using 20 allozyme loci. The mean number of alleles per locus was 2.63, the percent of polymorphic loci was 88.1%, the observed heterozygosity was 0.181, and the mean value of expected heterozygosity amount...

journal_title：Biochemical genetics

authors： Potenko VV

更新日期：2007-04-01 00:00:00

abstract：:Two complementing loci in different linkage groups of the basidiomycete Ustilago violacea are involved in urease activity: a structural one (ure-1) and a second inferred to involve a permease (ure-2) locus. Two types of complementing mutations occur in the structural locus: null activity (ure-la) and obviously reduced...

journal_title：Biochemical genetics

authors： Baird ML,Garber ED

更新日期：1981-12-01 00:00:00

abstract：:The structural gene locus specifying subunits of cytoplasmic isoenzymes of phosphoglucose isomerase (PGI) is present in duplicate in many diploid species of Clarkia (Onagraceae), a genus of annual plants native to California. We studied the kinetic properties and molecular weights of a large number of genetically defi...

journal_title：Biochemical genetics

authors： Gottlieb LD,Greve LC

更新日期：1981-02-01 00:00:00

abstract：:Using two-dimensional gel electrophoresis, we have analyzed proteins from a plasma membrane-enriched fraction from Phycomyces sporangiophores. Specifically, we have compared gels for night-blind mutants and a wild-type strain to find proteins involved in the early steps of the sensory transduction chain for phototropi...

journal_title：Biochemical genetics

authors： Pollock JA,Lipson ED,Sullivan DT

更新日期：1985-06-01 00:00:00

abstract：:When the 4-bp Dam recognition sequence was placed between two d(GA)7 tracts, it became severely undermethylated in JM101 Escherichia coli cells compared to other Dam sequences in the same plasmid DNA. This site specific undermethylation was also detected on supercoiled molecules in vitro. Mutational analysis indicated...

journal_title：Biochemical genetics

authors： Klysik J

更新日期：1996-06-01 00:00:00

abstract：:Imprinted genes are characterized by monoallelic expression that is dependent on parental origin. Comparative analysis of imprinted genes between species is a powerful tool for understanding the biological significance of genomic imprinting. The slc38a4 gene encodes a neutral amino acid transporter and is identified a...

journal_title：Biochemical genetics

authors： Xu D,Zhang C,Li J,Wang G,Chen W,Li D,Li S

更新日期：2018-12-01 00:00:00

abstract：:Non-alcoholic fatty liver disease (NAFLD) have a high prevalence in humans in the past two decades. Here, we elucidated the functions of miR-30a-3p in the development of NAFLD and identified its potential targets. HepG-2 cells and NAFLD patients' blood samples were used in our study. Bioinformatics analysis as well as...

journal_title：Biochemical genetics

authors： Wang DR,Wang B,Yang M,Liu ZL,Sun J,Wang Y,Sun H,Xie LJ

更新日期：2020-10-01 00:00:00

abstract：:A survey of laboratory strains of Anopheles stephensi for nonspecific esterases by polyacrylamide gel electrophoresis revealed 10 zones of esterase activity. In 3 of the 10 zones, three electromorphs were observed. Genetic analysis revealed that these three zones are controlled by three loci, viz., Est-3, Est-4, and E...

journal_title：Biochemical genetics

authors： Adak T,Subbarao SK,Sharma VP

更新日期：1984-06-01 00:00:00

abstract：:In corn seedlings (Zea mays L.) homozygous for the mutation gl5, the surface waxes are characteristically altered. In this mutant the main wax constituents (83.5%) are aldehydes while in the normal waxes alcohols predominate (62.7%). Moreover, in the normal waxes aldehydes and alcohols are made up mainly of the C32 te...

journal_title：Biochemical genetics

authors： Bianchi G,Avato P,Salamini F

更新日期：1978-10-01 00:00:00

abstract：:A plausible approach to evaluate the inhibitory action of antifungals is through the investigation of the fungal resistance to these drugs. We describe here the molecular cloning and initial characterization of the A. nidulans lipA gene, where mutation (lipA1) conferred resistance to undecanoic acid, the most fungitox...

journal_title：Biochemical genetics

authors： Brito-Madurro AG,Prade RA,Madurro JM,Santos MA,Peres NT,Cursino-Santos JR,Martinez-Rossi NM,Rossi A

更新日期：2008-10-01 00:00:00

abstract：:Electrophoretic variation of sorbitol dehydrogenase has been found in Mus musculus; the SDH-1 A phenotype migrates further toward the cathode under the conditions used than the SDH-1B phenotype. Mice with the SDH-1B phenotype have enzyme activity reduced to 25% of that found in mice with the SDH-1A phenotype, but no d...

journal_title：Biochemical genetics

authors： Andrews SJ,Peters J

更新日期：1983-08-01 00:00:00

abstract：:The inducibility of alcohol dehydrogenase (ADH) has been recognized in different systems including maize, Drosophila, and mice. Our earlier results showed strain-specific ADH responses to chronic ethanol administration relative to matched littermate controls in mice. For this study we used two strains which showed "in...

journal_title：Biochemical genetics

authors： Wang CH,Singh SM

更新日期：1984-08-01 00:00:00

abstract：:The secretogranin II (SCG2) gene is associated with the synthesis and secretion of follicle-stimulating hormone and luteinizing hormone. In the present study, we have determined the complete cDNA sequence of pig SCG2, which was submitted to GenBank with accession no. AY870646. Its complete open reading frame of 1,851 ...

journal_title：Biochemical genetics

authors： Du HL,Chen J,Zhang YS,Zhang XQ

更新日期：2008-06-01 00:00:00

abstract：:Considering that the genetically modified soybean lectin gene is affected by the gene type, to improve the stability of the genetically modified soybean lectin gene, a method based on fluorescence quantitative PCR to analyze the genetic characteristics of the genetically modified soybean lectin was proposed. The commo...

journal_title：Biochemical genetics

authors： Wang Z,Jia H,Yu H,Wang T,Yang B

更新日期：2020-09-26 00:00:00

abstract：:Because alternative RNA splicing regulation in the testis is prevalent, we explored testes of Sprague-Dawley rats for existence of alternatively spliced colony-stimulating factor 1 receptor (CSF-1R) mRNA. Using RT-PCR and sequencing, we identified a variant of CSF-1R mRNA that was 284 bp shorter than the full-length C...

journal_title：Biochemical genetics

authors： Su H,Wang Y,Söder O,Hou M

更新日期：2014-06-01 00:00:00

abstract：:In this study, we identified porcine single nucleotide polymorphisms (SNPs) by aligning eight sequences generated with two approaches: amplification of 665 intronic regions using one sample from each of eight breeds, including three East Asian pigs, and amplification of 289 3'-UTR regions using two samples from each o...

journal_title：Biochemical genetics

authors： Matsumoto T,Okumura N,Uenishi H,Hayashi T,Hamasima N,Awata T

更新日期：2012-06-01 00:00:00

abstract：:Starch gel electrophoresis and histochemical staining with L-leucylglycyl-glycine revealed genetic polymorphism in peptidase B in cattle erythrocytes. Genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, PepB1 and PepB2. A...

journal_title：Biochemical genetics

authors： Del Lama SN,Del Lama MA,Mestriner MA,Mortari N

更新日期：1992-06-01 00:00:00

abstract：:To elucidate the phylogeny of the genus Paramesotriton (Caudata: Salamandridae), we investigated three mitochondrial DNA gene fragments (1207 bp in total) of cytochrome b, ND2, and ND4 for its six recognized species. The phylogenetic relationships within Paramesotriton were reconstructed by maximum parsimony (MP) and ...

journal_title：Biochemical genetics

authors： Lu S,Yuan ZG,Pang J,Yang D,Yu F,McGuire P,Xie F,Zhang YP

更新日期：2004-06-01 00:00:00

abstract：:The genetic diversity of the Turkish native chicken breeds Denizli and Gerze was evaluated with 10 microsatellite markers. We genotyped a total of 125 individuals from five subpopulations. Among loci, the mean number of alleles was 7.5, expected heterozygosity (H (e)) was 0.665, PIC value was 0.610, and Wright's fixat...

journal_title：Biochemical genetics

authors： Kaya M,Yildiz MA

更新日期：2008-08-01 00:00:00

abstract：:We have shown in our previous work that in the tetraploid loach Misgurnus fossilis carboxylesterase-2 (E-2) phenotypes of embryos and early larvae are controlled by the alleles of the Est-2 locus. In the present work it has been established that the somatic tissues of many adult fish homozygous for one of the Est-2 al...

journal_title：Biochemical genetics

authors： Ivanenkov VV

更新日期：1983-06-01 00:00:00

abstract：:Techniques for performing numerous enzyme kinetic assays with minimum time and effort would be valuable to studies of the evolutionary genetics of metabolic control and the quantitative genetics of determinants of kinetic parameters. Microtiter plate readers have been used for a variety of repetitious analytical techn...

journal_title：Biochemical genetics

authors： Clark AG,Keith LE

更新日期：1989-06-01 00:00:00

abstract：:The frequency distributions of apolipoprotein C alleles in native Asian sheep (Bengal, Kagi, Lampuchhre, Vietnamese, Myanmar, Baruwal, Bhyanglung, Khalkhas, and Kuwait) were determined using a one-dimensional polyacrylamide gel isoelectric-focusing immunoblotting technique. Among these Asian sheep, the genetic locus c...

journal_title：Biochemical genetics

authors： Tsunoda K,Watanabe S,Sato J,Sato K

更新日期：2002-12-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) hypervariable segment I sequences (HVSI, 471 bp) of the control region and partial cytochrome b sequences (Cytb, 403 bp) were analyzed in three tentative species of the genus Mystacoleucus in China (M. chilopterus, M. marginatus, and M. lepturus). Not more than two mutations were found in bot...

journal_title：Biochemical genetics

authors： Kong QP,Yao YG,Huang SY,Huang JF,Zhang YP

更新日期：2003-10-01 00:00:00

abstract：:The microRNAs (miRNAs) are involved in multiple pathological processes among various types of tumors. However, the functions of miRNAs in benign brain tumors are largely unexplored. In order to explore the pathogenesis of the invasiveness in non-functional pituitary adenoma (NFPA), the miRNAs expression profile was an...

journal_title：Biochemical genetics

authors： Wu S,Gu Y,Huang Y,Wong TC,Ding H,Liu T,Zhang Y,Zhang X

更新日期：2017-06-01 00:00:00

abstract：:Thirteen isolates of porcine reproductive and respiratory syndrome virus (PRRSV) from different provinces of China were studied and compared with several PRRSV isolates from other countries. Phylogenetic analysis shows that all Chinese isolates of PRRSV in this study belong to the American genotype, except for one str...

journal_title：Biochemical genetics

authors： Chen J,Liu T,Zhu CG,Jin YF,Zhang YZ

更新日期：2006-10-01 00:00:00

abstract：:Melanoma formation in Xiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by the Tu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecular identification of this regulatory locus (R) r...

journal_title：Biochemical genetics

authors： Förnzler D,Wittbrodt J,Schartl M

更新日期：1991-12-01 00:00:00

abstract：:Genetic variation of a codominantly inherited pancreas protease, designated CTRA-1, was discovered in the house mouse by isoelectric focusing in polyacrylamide gels. Phenotype CTRA-1A was found in MOLH/Fre and in the majority of common laboratory mouse strains. Phenotype CTRA-1B was found in PWD/Ph. It was characteriz...

journal_title：Biochemical genetics

authors： von Deimling OH,Gröning J,Gaa A

更新日期：1991-12-01 00:00:00

abstract：:Patterns of lactate dehydrogenase (LDH) isozymes of tissues from various mouse strains were examined. An interstrain polymorphism for LDH isozymes of liver was established. One phenotype (CBA/Lac and AKR/J mice) yielded a five-banded LDH pattern, another phenotype (DBA/1J, DBA/2J, C57BL/6J and C3H/He) showed a three-b...

journal_title：Biochemical genetics

authors： Khlebodarova TM,Serov OL

更新日期：1980-10-01 00:00:00

abstract：:High levels of nuclease activities were identified in filtrates of Aspergillus cultures after growth in low-but not in high-phosphate media. Deoxyribonuclease activities, characterized extensively by column chromatography, showed a coincident single peak for ss- and ds-DNase which was distinct from the peak for RNase....

journal_title：Biochemical genetics

authors： Käfer E,Tittler A,Fraser MJ

更新日期：1989-04-01 00:00:00