Gene Analysis of Genetically Modified Soybean Lectin Based on Fluorescence Quantitative PCR.

abstract：:Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patter...

journal_title：Biochemical genetics

authors： Yee EY,Zainuddin ZZ,Ismail A,Yap CK,Tan SG

更新日期：2013-10-01 00:00:00

abstract：:A partial paired gene of Drosophila willistoni containing the paired box and extended homeo box was amplified by PCR and the nucleotide sequence of 1141 bp was determined. Comparison of the paired genes in D. willistoni and D. melanogaster showed that the proportions of identical nucleotide sites in the coding region ...

journal_title：Biochemical genetics

authors： Matsuo Y

更新日期：1996-12-01 00:00:00

abstract：:Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) exhibit genetic polymorphism and tissue specificity. ADH and ALDH isozyme phenotypes from 39 surgical Chinese lung specimens were identified by agarose isoelectric focusing. The identity of the lung beta-ADHs was further demonstrated by their characteristic...

journal_title：Biochemical genetics

authors： Yin SJ,Liao CS,Chen CM,Fan FT,Lee SC

更新日期：1992-04-01 00:00:00

abstract：:Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the ca...

journal_title：Biochemical genetics

authors： Jorge IC,Mangolin CA,Machado MF

更新日期：1997-06-01 00:00:00

abstract：:Genetic variations affecting the course of depressive symptoms in patients with coronary artery disease (CAD) have not yet been well studied. Therefore, we set out to investigate whether distinct haplotypes of the two insertion/deletion polymorphisms in the serotonin-transporter-linked polymorphic region (5-HTTLPR) an...

journal_title：Biochemical genetics

authors： Meyer T,Rothe I,Staab J,Deter HC,Fangauf SV,Hamacher S,Hellmich M,Jünger J,Ladwig KH,Michal M,Petrowski K,Ronel J,Söllner W,Weber C,de Zwaan M,Williams RB,Albus C,Herrmann-Lingen C,SPIRR-CAD Investigators.

更新日期：2020-08-01 00:00:00

abstract：:A new variant of hereditary hemolytic anemia in a family due to high adenosine triphosphate (ATP) is reported. The increase in ATP levels varied from 83 to 105% in the family members. Low 2,3-diphosphoglycerate levels and low 2,3-diphosphoglyceromutase activity were observed in three family members, with normal glucos...

journal_title：Biochemical genetics

authors： Bapat JP,Baxi AJ

更新日期：1981-10-01 00:00:00

abstract：:Congenital heart disease is the most common congenital anomaly, representing an important cause of infant morbidity and mortality. Congenital heart disease represents a group of heart anomalies that include septal defects, valve defects, and outflow tract anomalies. The exact genetic, epigenetic, or environmental basi...

journal_title：Biochemical genetics

authors： Muntean I,Togănel R,Benedek T

更新日期：2017-04-01 00:00:00

abstract：:The tissue distribution of activin receptor-like kinase 7 (Alk7) expression, the signaling ability of Alk7 variants, and Alk7 expression in response to β3-adrenergic receptor activation were examined. Expression levels of Alk7 varied greatly among tissues but were highest in white adipose tissue and brown adipose tiss...

journal_title：Biochemical genetics

authors： Murakami M,Shirai M,Ooishi R,Tsuburaya A,Asai K,Hashimoto O,Ogawa K,Nishino Y,Funaba M

更新日期：2013-04-01 00:00:00

abstract：:Genetic analysis of a polymorphic tissue esterase revealed a new locus (Est-6) with two alleles (Est-6a and Est-6b) on linkage group VI of the rabbit. Est-6 is closely linked to the Est-1,2,4 cluster. Esterase of Est-6 is found in many organs, particularly in liver and small intestine, but not in erythrocytes and seru...

journal_title：Biochemical genetics

authors： van Zutphen LF,den Bieman MG,von Deimling O,Fox RR

更新日期：1987-06-01 00:00:00

abstract：:When the 4-bp Dam recognition sequence was placed between two d(GA)7 tracts, it became severely undermethylated in JM101 Escherichia coli cells compared to other Dam sequences in the same plasmid DNA. This site specific undermethylation was also detected on supercoiled molecules in vitro. Mutational analysis indicated...

journal_title：Biochemical genetics

authors： Klysik J

更新日期：1996-06-01 00:00:00

abstract：:A bioautographic procedure has been developed for the visualization of the isozymes of dihydrofolate reductase (DHFR, E.C. 1.5.1.3). In addition to detecting electrophoretically separated enzymes, bioautography was utilized to visualize DHFR after isoelectric focusing on polyacrylamide gels. Both zone electrophoresis ...

journal_title：Biochemical genetics

authors： Naylor SL,Townsend JK,Klebe RJ

更新日期：1980-02-01 00:00:00

abstract：:The frequency distributions of apolipoprotein C alleles in native Asian sheep (Bengal, Kagi, Lampuchhre, Vietnamese, Myanmar, Baruwal, Bhyanglung, Khalkhas, and Kuwait) were determined using a one-dimensional polyacrylamide gel isoelectric-focusing immunoblotting technique. Among these Asian sheep, the genetic locus c...

journal_title：Biochemical genetics

authors： Tsunoda K,Watanabe S,Sato J,Sato K

更新日期：2002-12-01 00:00:00

abstract：:Propionyl-CoA carboxylase (PCC) is involved in the catabolism of branched chain amino acids, odd-numbered fatty acids, cholesterol, and other metabolites. PCC consists of two subunits, α and β, encoded by the PCCA and PCCB genes, respectively. Mutations in the PCCA or PCCB subunit gene may lead to propionic acidemia. ...

journal_title：Biochemical genetics

authors： Chiu YH,Liu YN,Liao WL,Chang YC,Lin SP,Hsu CC,Chiu PC,Niu DM,Wang CH,Ke YY,Chien YH,Hsiao KJ,Liu TT

更新日期：2014-10-01 00:00:00

abstract：:The genetic divergence, population genetic structure, and possible speciation of the Korean firefly, Pyrocoelia rufa, were investigated on the midsouthern Korean mainland, coastal islets, a remote offshore island, Jedu-do, and Tsushima Island in Japan. Analysis of DNA sequences from the mitochondrial COI protein-codin...

journal_title：Biochemical genetics

authors： Lee SC,Bae JS,Kim I,Suzuki H,Kim SR,Kim JG,Kim KY,Yang WJ,Lee SM,Sohn HD,Jin BR

更新日期：2003-12-01 00:00:00

abstract：:A variant of fetal hemoglobin (Hb F-Malta-I) has been detected and quantitated in adult blood with a sensitive radioimmunoassay employing monospecific antisera. The concentration of Hb-F-Malta-I was 0.002-0.05%, with an average value of 0.011%. The ratio of Hb F-Malta-I/Hb F in adults was about 4.8%, compared to a rat...

journal_title：Biochemical genetics

authors： Altay G,Garver F,Bannister WH,Grech JL,Felice A,Huisman TH

更新日期：1977-10-01 00:00:00

abstract：:A survey of 37 inbred strains for erythrocyte purine nucleoside phosphorylase activity showed a greater than threefold range. Six of these strains had significantly greater activity than the others, and all of the high-activity strains had the Np-2 electrophoretic band. The high-purine nucleoside phosphorylase activit...

journal_title：Biochemical genetics

authors： Snyder FF,Biddle FG,Lukey T,Sparling MJ

更新日期：1983-04-01 00:00:00

abstract：:Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multip...

journal_title：Biochemical genetics

authors： Wu N,Li CJ,Gui JF

更新日期：2009-12-01 00:00:00

abstract：:Akt isoforms have critical roles in the cause and regulation of cancer cells invasive, migration, and metastatic dissemination. In the present study, the association between Akt1 polymorphisms and endometrial cancer was investigated in patients with endometrial cancer and controls. Thirty premenopaused patients diagno...

journal_title：Biochemical genetics

authors： Fallah S,Korani M,Hajimirza M,Seifi M

更新日期：2015-12-01 00:00:00

abstract：:Isoenzyme electrophoresis was used as a method to determine genetic diversity in various M. esculenta cultivars collected in the Southwestern (SW) and Northwestern (NW) regions of the State of Parana, in the South region of Brazil, and in cultivars produced at the Agronomic Institute of Campinas (IAC), São Paulo State...

journal_title：Biochemical genetics

authors： Resende AG,Vidigal Filho P,Machado MF

更新日期：2000-08-01 00:00:00

abstract：:We have examined the linkage relationships between five biochemical markers, Acon-1, Ahd-2, Ahd-c, Akp-1, and Hao-1, and 19 other genetic loci in five breeding combinations. The genetic locus that codes for a recently described aldehyde dehydrogenase in the liver (Ahd-c) has been assigned to linkage group X (LG X). Hy...

journal_title：Biochemical genetics

authors： Cramer DV,Mowery PA,Adams M

更新日期：1986-04-01 00:00:00

abstract：:Investigations of an euploid series of castor bean, Ricinus communis L., Consisting of haploid, diploid, and tetraploid individuals, was performed to determine the value of such a series in studying the biochemical consequences of genome multiplication. The effects of euploidization of the nuclear genome on the biosyn...

journal_title：Biochemical genetics

authors： Timko MP,Vasconcelos AC,Fairbrothers DE

更新日期：1980-02-01 00:00:00

abstract：:We extracted the yellow melanin (phaeomelanin), black melanin (eumelanin), and mixed type of melanin from dorsal hair of dominant yellow (Ay/a), non-agouti (a/a), and agouti (A/A) mice, respectively. Spectrophotometric and fluorescence spectrophotometric analysis demonstrated that the yellow melanin was qualitatively ...

journal_title：Biochemical genetics

authors： Ikejima T,Takeuchi T

更新日期：1978-08-01 00:00:00

abstract：:A null mutation at the phosphoglucomutase locus (Pgm-1) was discovered by electrophoretic analysis of the inbred mouse strain C57BL/6J. The null allele (Pgm-1n) was shown to segregate as a Mendelian unit alternative to the Pgm-1a and Pgm-1b alleles. Mice expressing the Pgm-1n allele, either in the heterozygous or homo...

journal_title：Biochemical genetics

authors： Johnson FM,Hendren RW,Chasalow F,Barnett LB,Lewis SE

更新日期：1981-06-01 00:00:00

abstract：:Single-chain variable fragment (scFv) is one of the most common antibody forms. This report describes the expression of the scFv gene as a soluble protein in Origami DE3 cytoplasm. The purified scFv recognized the epidermal growth factor receptor (EGFRvIII) on the surface of MCF-7 cells. The scFv protein was purified ...

journal_title：Biochemical genetics

authors： Mahgoub IO

更新日期：2012-08-01 00:00:00

abstract：:An anonymous honey bee locus, detected previously with a cloned probe, has HhaI RFLP alleles specific to African bees or common to both African and European bees. To facilitate identification of these alleles, this region, 1231, was made analyzable with the PCR. The two halves of the region, excluding the termini, wer...

journal_title：Biochemical genetics

authors： Hall HG

更新日期：1998-10-01 00:00:00

abstract：:Segregation and linkage relationship of nine allozyme loci, which are fixed for alternative alleles in the European fire-bellied toads, Bombinal bombina and B. variegata, were studied using artificial F1 hybrids to obtain backcross and F2 progeny. Alleles coding for electromorphs at nine loci (Ldh-1, Mdh-1, Idh-1, Ck,...

journal_title：Biochemical genetics

authors： Hofman S,Szymura JM

更新日期：2000-08-01 00:00:00

abstract：:Pancreatic cancer is the fourth leading cause of death in both males and females, with a 5-year relative survival rate of 8%. The Wnt signaling pathway has a significant role in the pathogenesis of many tumors, including those of pancreatic cancer. Hypermethylation of the Wnt inhibitory Factor-1 (WIF1) gene promoter h...

journal_title：Biochemical genetics

authors： Rahmani B,Hamedi Asl D,Naserpour Farivar T,Azad M,Sahmani M,Gheibi N

更新日期：2019-08-01 00:00:00

abstract：:Non-alcoholic fatty liver disease (NAFLD) have a high prevalence in humans in the past two decades. Here, we elucidated the functions of miR-30a-3p in the development of NAFLD and identified its potential targets. HepG-2 cells and NAFLD patients' blood samples were used in our study. Bioinformatics analysis as well as...

journal_title：Biochemical genetics

authors： Wang DR,Wang B,Yang M,Liu ZL,Sun J,Wang Y,Sun H,Xie LJ

更新日期：2020-10-01 00:00:00

abstract：:The present study intends to survey the genetic variability of an endangered semiferal Portuguese native pony breed, the Garrano. Thirteen microsatellite markers were examined in 277 animals born in 1998, belonging to eight subpopulations corresponding to eight northern Portuguese geographic regions. Mean heterozygosi...

journal_title：Biochemical genetics

authors： Morais J,Oom MM,Malta-Vacas J,Luís C

更新日期：2005-08-01 00:00:00

abstract：:The aryl hydrocarbon hydroxylase (Ah) locus that controls the induction of chemical carcinogen-metabolizing enzymes in mice has been found to be linked to a new restriction-fragment length polymorphism (RFLP). Only C57BL/6 and closely related inbred strains displayed a 7.6-kb HindIII restriction fragment, while all ot...

journal_title：Biochemical genetics

authors： Cobb RR,Stoming TA,Whitney JB 3rd

更新日期：1987-06-01 00:00:00