Abstract:
:Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the callus tissues were transferred to medium maintained at 22 or 37 degrees C and containing different concentrations of sucrose, glucose, and fructose, the different combinations of five mitochondrial MDH (mtMDH) and two micro-body MDH (mbMDH) showed different MDH isozyme patterns in the callus populations. Differential expression of mtMDH isozymes seems to be modulated at the posttranslational level in callus tissues exposed to different concentrations and types of sugar and to high-temperature and low-temperature stress. An inductor effect on the expression of mbMDH isozymes was observed under stress conditions and in long-term callus tissue, and they may also present different responses.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Jorge IC,Mangolin CA,Machado MFdoi
10.1023/a:1021950008544subject
Has Abstractpub_date
1997-06-01 00:00:00pages
155-64issue
5-6eissn
0006-2928issn
1573-4927journal_volume
35pub_type
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