Rapid enzyme kinetic assays of individual Drosophila and comparisons of field-caught D. melanogaster and D. simulans.

abstract：:Larval serum protein-1 (LSP-1) and LSP-2 are the major proteins of Drosophila larval serum. The amount of LSP-1 synthesized is strictly proportional to the number of LSP-1 genes present within the range 1-10. The normal number in female flies is 6. Flies with extreme amounts of LSP-1 were, by our criteria, as fit as t...

journal_title：Biochemical genetics

authors： Roberts DB,Blackwell SA,Loughlin SA

更新日期：1984-10-01 00:00:00

abstract：:The immunological cross-reactivity of heterogeneous acid phosphatase isozymes from different human tissues has been studied using monospecific antisera prepared against four homogeneous acid phosphatases. The enzyme characterized as tartrate-inhibitable, prostatic acid phosphatase is also found to be present in leukoc...

journal_title：Biochemical genetics

authors： Waheed A,Van Etten RL,Gieselmann V,von Figura K

更新日期：1985-04-01 00:00:00

abstract：:Alcohol dehydrogenase class IV (ADH4) participates in retinol metabolism and is expressed primarily in ocular, digestive, and reproductive tissues of the mouse. A naturally occurring genetic variant in C57BL/6J mice results in a faster migrating ADH4 enzyme during electrophoresis when compared to other non-C57BJ/6J st...

journal_title：Biochemical genetics

authors： Dolney DE,Szalai G,Felder MR

更新日期：2001-08-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) hypervariable segment I sequences (HVSI, 471 bp) of the control region and partial cytochrome b sequences (Cytb, 403 bp) were analyzed in three tentative species of the genus Mystacoleucus in China (M. chilopterus, M. marginatus, and M. lepturus). Not more than two mutations were found in bot...

journal_title：Biochemical genetics

authors： Kong QP,Yao YG,Huang SY,Huang JF,Zhang YP

更新日期：2003-10-01 00:00:00

abstract：:The Wood Stork (Mycteria americana) is a colonial wading bird of the tropical and lower subtropical zones. We assessed genetic structure within and among five stork colonies from the Brazilian Pantanal and compared our data with those from North American populations. Samples of 234 individuals were studied using prote...

journal_title：Biochemical genetics

authors： Del Lama SN,Lopes IF,Del Lama MA

更新日期：2002-04-01 00:00:00

abstract：:The stem-loop potential of a nucleic acid segment (expressed as a FONS value), decomposes into base composition-dependent and base order-dependent components. The latter, expressed as a FORS-D value, is derived by subtracting the value of the base composition-dependent component (FORS-M) from the FONS value. FORS-D an...

journal_title：Biochemical genetics

authors： Zhang CY,Wei JF,Wu JS,Xu WR,Sun X,He SH

更新日期：2008-02-01 00:00:00

abstract：:In flowers of Dianthus caryophyllus (carnation), the gene I is concerned with a discrete step in flavonoid biosynthesis, Genotypes with recessive (ii) alleles produce yellow flowers, which contain the chalcone isosalipurposide (naringenin-chalcone-2'-glucoside) as the major petal pigment, but in genotypes with wild-ty...

journal_title：Biochemical genetics

authors： Forkmann G,Dangelmayr B

更新日期：1980-06-01 00:00:00

abstract：:Prolamin mixtures were isolated from oats, rice, normal and high-lysine sorghum, two varieties of pearl millet, two strains of teosinte, and gamma grass and subjected to NH2-terminal amino acid sequence determinations. In each case (except for rice, whose prolamins apparently have blocked or unavailable NH2-terminal r...

journal_title：Biochemical genetics

authors： Bietz JA

更新日期：1982-12-01 00:00:00

abstract：:In this study, the geographic patterns of genetic variation of three rodent species belonging to the tribe Oryzomyini were investigated using the mitochondrial cytochrome b and nuclear IRBP genes in biomes that are undergoing degradation processes to a greater or lesser degree. The samples are from 25 collecting local...

journal_title：Biochemical genetics

authors： Miranda GB,Andrades-Miranda J,Oliveira LF,Langguth A,Mattevi MS

更新日期：2007-12-01 00:00:00

abstract：:Electrophoretic variation of sorbitol dehydrogenase has been found in Mus musculus; the SDH-1 A phenotype migrates further toward the cathode under the conditions used than the SDH-1B phenotype. Mice with the SDH-1B phenotype have enzyme activity reduced to 25% of that found in mice with the SDH-1A phenotype, but no d...

journal_title：Biochemical genetics

authors： Andrews SJ,Peters J

更新日期：1983-08-01 00:00:00

abstract：:Aggrecan is the major component of intervertebral disk matrix proteoglycan with multiple functional domains. To understand the role of aggrecan polymorphism in a part of exon 12 encoding the CS1 domain in lumbar disk degeneration disease, we have analyzed genomic DNA from 71 patients with the disease and 108 healthy i...

journal_title：Biochemical genetics

authors： Mashayekhi F,Shafiee G,Kazemi M,Dolati P

更新日期：2010-08-01 00:00:00

abstract：:Seasonal changes in genetic diversity of Meriones meridianus and Dipus sagitta populations in the Ningxia Hui Autonomous Region were monitored using inter-simple sequence repeats. There were 45 discernible DNA fragments isolated from 33 samples of M. meridianus, and 25 from 40 samples of D. sagitta. The differences in...

journal_title：Biochemical genetics

authors： Zhou R,Li Y,Li JQ,Liu NF

更新日期：2012-06-01 00:00:00

abstract：:Chronic myeloid leukemia (CML) is a hematopoietic stem cell malignancy characterized by the expression of the BCR-ABL1 fusion gene with different chimeric transcripts. Despite the crucial impact of constitutively active tyrosine kinase in CML pathogenesis, aberrant DNA methylation of certain genes plays an important r...

journal_title：Biochemical genetics

authors： Behzad MM,Shahrabi S,Jaseb K,Bertacchini J,Ketabchi N,Saki N

更新日期：2018-06-01 00:00:00

abstract：:The molecular weight forms of kynurenine formamidase were studied both gentically and biochemically. Formamidase I (native molecular weight 60,000) was purified using (NH4)2SO4 and pH fractionation, DEAE-cellulose chromatography at two different pH's, hydroxylapatite chromatography, and Sephadex G-100 gel filtration. ...

journal_title：Biochemical genetics

authors： Moore GP,Sullivan DT

更新日期：1978-08-01 00:00:00

abstract：:Insulin-like growth factor (IGF-I) is an important growth factor in mammals, but the functions of the local muscle-specific isoform of insulin-like growth factor 1 (mIGF-1) to skeletal muscle development have rarely been reported. To determine the effect of pig mIGF-1 on body development and muscle deposition in vivo ...

journal_title：Biochemical genetics

authors： Gao B,Wang W,Wu H,Chen C,Shen D,Wang S,Chen W,Zhang L,Chan S,Song C

更新日期：2018-08-01 00:00:00

abstract：:A new variant of alcohol dehydrogenase (ADH 7lk) was found in a laboratory stock of Drosophila melanogaster. ADH in this stock had the same electrophoretic mobility as the F variant both on acrylamide and on agar. Activity levels were similar to the levels in F flies at temperature between 15 and 25 C. But while ADH F...

journal_title：Biochemical genetics

authors： Thörig EW,Schoone AA,Scharloo W

更新日期：1975-10-01 00:00:00

abstract：:The RAPD method was used to assess the genetic differentiation of brown hare (Lepus europaeus) populations from Central Greece. Greek wild populations were compared with samples from Austria, Poland, Germany, France, and Bulgaria, as well as with reared/released hares to investigate the impact of the releases on the n...

journal_title：Biochemical genetics

authors： Mamuris Z,Sfougaris AI,Stamatis C,Suchentrunk F

更新日期：2002-10-01 00:00:00

abstract：:Glutamate-oxaloacetate transaminase (GOT; EC 2.6.1.1) occurs as two electrophoretically distinguishable isozymes in the copepod Tigriopus californicus. The slower-migrating form, referred to as GOT2 , is shown to be associated with the mitochondrial cell fraction. GOT2 phenotypes are inherited in typical Mendelian fas...

journal_title：Biochemical genetics

authors： Dill MM,Burton RS

更新日期：1984-04-01 00:00:00

abstract：:It is shown that the gene controlling the synthesis of the organ-specific S-esterase of Drosophila virilis ejaculatory bulbs is located on the second chromosome (at approximate position 192.1 +/- map units). The cells of the genital imaginal disks are determined for the synthesis of S-esterase 10-12 hr after the secon...

journal_title：Biochemical genetics

authors： Korochkin LI,Matveeva NM,Kuzin BA,Karasik GI,Maximovsky LF

更新日期：1978-08-01 00:00:00

abstract：:The single-strand conformation polymorphism (SSCP) procedure has been applied in routine testing for hereditary diseases. Temperature, running buffer, gel composition, and fragment length can influence its sensitivity. Mutation detection in the clinical setting depends on the development of automated technology, espec...

journal_title：Biochemical genetics

authors： Okamoto Y,Ueta A,Sumi S,Ito T,Okubo Y,Jose Y,Ninomiya A,Togari H,Nishida M

更新日期：2007-10-01 00:00:00

abstract：:A genetic locus controlling the electrophoretic mobility of a methylglyoxal dehydrogenase (EC 1.2.1.23) in the rat is described. The locus, designated Mgd1, is expressed in liver and kidney. Inbred rat strains have fixed either allele Mgd1a or allele Mgd1b. Codominant expression is observed in heterozygotes, providing...

journal_title：Biochemical genetics

authors： Bender K,Seibert RT,Wienker TF,Kren V,Pravenec M,Bissbort S

更新日期：1994-06-01 00:00:00

abstract：:The taxonomy of the genus Hypostomus in the upper Rio Paraná basin presents some very complex issues. For this reason, an allozyme electrophoresis survey of 10 populations of Hypostomus from the Rio Corumbá basin was performed, revealing 25 loci from 14 enzyme systems. Allozyme data revealed diagnostic genetic markers...

journal_title：Biochemical genetics

authors： Zawadzki CH,Renesto E,Mateus RP

更新日期：2008-12-01 00:00:00

abstract：:A method for detecting two alleles at Np-1 (nucleoside phosphorylase) and three alleles at Es-10 (esterase 10) from mouse blood by cellulose acetate electrophoresis is described. The allelic constitution at these loci for 44 inbred strains and stocks was determined. The location of Np-1 on chromosome 14 was establishe...

journal_title：Biochemical genetics

authors： Womack JE,Davisson MT,Eicher EM,Kendall DA

更新日期：1977-04-01 00:00:00

abstract：:Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated...

journal_title：Biochemical genetics

authors： Zagris N

更新日期：1977-10-01 00:00:00

abstract：:Polymorphic esterase and acetylcholinesterase alleles in the whitefly Bemisia tabaci were studied using electrophoretic and colorimetric assays. The segregation of these alleles between parental and F1 generations provided unequivocal evidence of haplodiploidy in this pest species. Unmated females, heterozygous at a p...

journal_title：Biochemical genetics

authors： Byrne FJ,Devonshire AL

更新日期：1996-04-01 00:00:00

abstract：:Eukaryotic translation initiation factors (eIFs) have been shown to be critical in the initiation of protein synthesis. Here, we report the cloning and characterization of a novel gene, LceIF1, from a potentially interesting forage grass, Leymus chinensis (Trin.). The expression results show that LceIF1 is expressed i...

journal_title：Biochemical genetics

authors： Sun YL,Hong SK

更新日期：2013-02-01 00:00:00

abstract：:The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate, pH 3.1) and two-dimensional (first dimension, Al-lactate, pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the alpha zone...

journal_title：Biochemical genetics

authors： Kudryavtsev AM,Metakovsky EV,Sozinov AA

更新日期：1988-12-01 00:00:00

abstract：:Two species of the genus Taricha are widely distributed. T. granulosa ranges from southern Alaska to central California. T. torosa is comprised of two described subspecies, T. t. torosa, which occupies much of the coast ranges of California, and T. t. sierrae, which inhabits the western slopes of the Sierra Nevada Mou...

journal_title：Biochemical genetics

authors： Hedgecock D

更新日期：1976-08-01 00:00:00

abstract：:Electrophoretic variation is described for malic enzyme (ME) for the first time in brook trout (Salvelinus fontinalis). Since the quaternary structure of ME was not clear from examination of banding patterns in brook trout alone, ME phenotypes in rainbow trout (Salmo gairdneri)X brook trout hybrids as wel as in esoci...

journal_title：Biochemical genetics

authors： Stoneking M,May B,Wright JE Jr

更新日期：1979-08-01 00:00:00

abstract：:Neurospora crassa can utilize numerous compounds including certain amino acids as a sole nitrogen source. Mutants of the nit-2 locus, a regulatory gene which is postulated to mediate nitrogen catabolite repression, are deficient in the ability to utilize several amino acids as well as other nitrogen sources used by wi...

journal_title：Biochemical genetics

authors： Facklam TJ,Marzluf GA

更新日期：1978-04-01 00:00:00