Abstract：
:The molecular weight forms of kynurenine formamidase were studied both gentically and biochemically. Formamidase I (native molecular weight 60,000) was purified using (NH4)2SO4 and pH fractionation, DEAE-cellulose chromatography at two different pH's, hydroxylapatite chromatography, and Sephadex G-100 gel filtration. Its subunit molecular weight, as determined by SDS gel electrophoresis, is 34,000, indicating that formamidase I is a dimer. Its Km is 1.87 X 10(-3) M. Its isoelectric point is pH 5.3. Its amino acid composition is reported. Formamidase II (native molecular weight 31,000) was partially purified using techniques similar to those above. Its Km is 2.31 X 10(-3) M. The response of formamidase activity to change in gene dosage was measured in segmental aneuploids generated in the second, third, and X chromosomes. Two separate chromosomal regions were identified which when present in extra dosage result in an elevation of the level of formamidase activity close to that predicted for the addition of a structural gene in a two-gene system. These tentative map positions were substantiated by demonstration that addition of one of the regions, 25A-27E, causes a 50% elevation in the relative amount of formamidase II. Addition of the other region, 91B-93F, causes a similar elevation in the relative amount of formamidase I. A model of the evolutionary origin of the two forms is presented, and the significance of these results to this model is discussed.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Moore GP,Sullivan DTdoi
10.1007/BF00484718subject
Has Abstractpub_date
1978-08-01 00:00:00pages
619-34issue
7-8eissn
0006-2928issn
1573-4927journal_volume
16pub_type
杂志文章abstract：:Studies have investigated the association between angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and cardiometabolic risk factors (CMRFs), however with varying results, which could be due to ethnicity differences. Therefore, the present study was conducted among Bhil tribal population (...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-9845-x
更新日期：2018-08-01 00:00:00
abstract：:Panagrellus redivivus when placed in 7% ethanol or methanol becomes immobile. After 1 hr the animals resume normal swimming and will grow in alcohol. The ability to recover requires ADH activity and translation, but not transcription, as determined by inhibitor studies. Recovery decreases with longer-chain alcohols, w...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484508
更新日期：1977-12-01 00:00:00
abstract：:The alpha-amylase in Drosophila melanogaster is a highly polymorphic enzyme, at both the allozyme level and the specific activity level. This enzyme changes its specific activity drastically depending on both food conditions and developmental stages, and it has been suggested that the ability to change its activity de...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1018715528413
更新日期：1999-12-01 00:00:00
abstract：:Genetic linkage maps are valuable tools for genetic, genomic, and crop breeding studies. Several genetic linkage maps were constructed for the olive (Olea europaea L.) genome, mainly using amplified fragment length polymorphisms (AFLPs) and simple sequence repeat (SSR) markers. However, AFLPs and SSR markers were not ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-016-9721-5
更新日期：2016-06-01 00:00:00
abstract：:We have shown in our previous work that in the tetraploid loach Misgurnus fossilis carboxylesterase-2 (E-2) phenotypes of embryos and early larvae are controlled by the alleles of the Est-2 locus. In the present work it has been established that the somatic tissues of many adult fish homozygous for one of the Est-2 al...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484449
更新日期：1983-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02395516
更新日期：1988-12-01 00:00:00
abstract：:A major category of mutant hematopoietic phenotypes in Drosophila is melanotic tumors or nodules, which consist of abnormal and overproliferated blood cells, similar to granulomas. Our analyses of the melanotic mutant dappled have revealed a novel type of gene involved in blood cell regulation. The dappled gene is an ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9411-7
更新日期：2011-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-011-9486-9
更新日期：2012-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484518
更新日期：1984-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-006-9054-x
更新日期：2006-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504367
更新日期：1980-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-014-9649-6
更新日期：2014-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484561
更新日期：1977-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00502121
更新日期：1979-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00502977
更新日期：1986-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484946
更新日期：1982-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00498965
更新日期：1979-04-01 00:00:00
abstract：:The Wood Stork (Mycteria americana) is a colonial wading bird of the tropical and lower subtropical zones. We assessed genetic structure within and among five stork colonies from the Brazilian Pantanal and compared our data with those from North American populations. Samples of 234 individuals were studied using prote...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1015109321773
更新日期：2002-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483970
更新日期：1982-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484629
更新日期：1981-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1001963319965
更新日期：2000-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-016-9778-1
更新日期：2017-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-008-9164-8
更新日期：2008-08-01 00:00:00
abstract：:ZFY-like genes have been observed in a variety of vertebrate species. Although originally implicated as the primary testis-determining gene in humans and other placental mammals, more recent evidence indicates a role(s) outside that of testis determination. In this study, DNA from five species of fish, Carasius auratu...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9350-3
更新日期：2010-08-01 00:00:00
abstract：:In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene appears to play a role in nitrogen catabolite repression. Using the N. crassa nmr gene as a probe, homologous sequences were ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:Glycogen synthase, which catalyzes the synthesis of glycogen, is especially important for Old World (Pteropodidae) and New World (Phyllostomidae) fruit bats that ingest high-carbohydrate diets. Glycogen synthase 1, encoded by the Gys1 gene, is the glycogen synthase isozyme that functions in muscles. To determine wheth...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-014-9659-4
更新日期：2014-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1020781629510
更新日期：2002-12-01 00:00:00
abstract：:Single nucleotide polymorphisms (SNP) in the resistin gene (RETN) are linked to obesity and resistin levels in various populations. However, results have been inconsistent. This study aimed to investigate association between polymorphisms in the resistin gene with obesity in a homogenous Malaysian Malay population. Th...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-015-9678-9
更新日期：2015-06-01 00:00:00
abstract：:Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the ca...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1021950008544
更新日期：1997-06-01 00:00:00
