Identification of an isoform of colony-stimulating factor 1 receptor mRNA in the rat testis.

Abstract:

:Because alternative RNA splicing regulation in the testis is prevalent, we explored testes of Sprague-Dawley rats for existence of alternatively spliced colony-stimulating factor 1 receptor (CSF-1R) mRNA. Using RT-PCR and sequencing, we identified a variant of CSF-1R mRNA that was 284 bp shorter than the full-length CSF-1R transcript. This variant was present in the testis (late fetal stage to adult) and in other organs of rats (7 and 60 days old). The deletion of 284 bp disrupted the open reading frame, resulting in a noncoding mRNA product. When testicular macrophages were stimulated with CSF-1R ligand and lipopolysaccharide, proportionally increased expression of both short isoform and full-length CSF-1R mRNA was observed. Thus, the identified isoform of CSF-1R mRNA may interfere with the expression of full-length CSF-1R mRNA, thereby affecting the biological activity of the ligand/receptor signaling axis in Sprague-Dawley rats.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Su H,Wang Y,Söder O,Hou M

doi

10.1007/s10528-014-9649-6

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

310-9

issue

5-6

eissn

0006-2928

issn

1573-4927

journal_volume

52

pub_type

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