Abstract:
:Because alternative RNA splicing regulation in the testis is prevalent, we explored testes of Sprague-Dawley rats for existence of alternatively spliced colony-stimulating factor 1 receptor (CSF-1R) mRNA. Using RT-PCR and sequencing, we identified a variant of CSF-1R mRNA that was 284 bp shorter than the full-length CSF-1R transcript. This variant was present in the testis (late fetal stage to adult) and in other organs of rats (7 and 60 days old). The deletion of 284 bp disrupted the open reading frame, resulting in a noncoding mRNA product. When testicular macrophages were stimulated with CSF-1R ligand and lipopolysaccharide, proportionally increased expression of both short isoform and full-length CSF-1R mRNA was observed. Thus, the identified isoform of CSF-1R mRNA may interfere with the expression of full-length CSF-1R mRNA, thereby affecting the biological activity of the ligand/receptor signaling axis in Sprague-Dawley rats.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Su H,Wang Y,Söder O,Hou Mdoi
10.1007/s10528-014-9649-6subject
Has Abstractpub_date
2014-06-01 00:00:00pages
310-9issue
5-6eissn
0006-2928issn
1573-4927journal_volume
52pub_type
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journal_title:Biochemical genetics
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