Molecular characterization and functional commonality of nucleophosmin/nucleoplasmin in two cyprinid fish.

Abstract:

:Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Wu N,Li CJ,Gui JF

doi

10.1007/s10528-009-9274-y

subject

Has Abstract

pub_date

2009-12-01 00:00:00

pages

749-62

issue

11-12

eissn

0006-2928

issn

1573-4927

journal_volume

47

pub_type

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