Abstract:
:Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Wu N,Li CJ,Gui JFdoi
10.1007/s10528-009-9274-ysubject
Has Abstractpub_date
2009-12-01 00:00:00pages
749-62issue
11-12eissn
0006-2928issn
1573-4927journal_volume
47pub_type
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