Abstract：
:The alpha-amylase in Drosophila melanogaster is a highly polymorphic enzyme, at both the allozyme level and the specific activity level. This enzyme changes its specific activity drastically depending on both food conditions and developmental stages, and it has been suggested that the ability to change its activity depending on the source of food has positive correlation with fitness. But the cause of the difference of specific activity among strains and food compositions is not known. In order to investigate the cause of these differences, we measured both the specific activity of amylase and the relative amount of Amy mRNA in eight strains of D. melanogaster with different electromorphs, in two food environments and two developmental stages. We found the following. First, the food-dependent activity change is regulated at the transcription level. Second, there was a significant correlation between specific activity and mRNA level among strains. So 80 to 90% of the specific activity difference can be explained by differences in the level of mRNAs, but the remaining part cannot. Finally, there were significant differences in specific activity per mRNA both among strains and between developmental stages. This suggests that there are differences in the catalytic efficiency of each allozyme, strain- or stage-specific translation rate, enzyme stability, or differential use of two Amy loci.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Yamate N,Yamazaki Tdoi
10.1023/a:1018715528413subject
Has Abstractpub_date
1999-12-01 00:00:00pages
345-56issue
11-12eissn
0006-2928issn
1573-4927journal_volume
37pub_type
杂志文章abstract：:Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia associated with insulin resistance and relative insulin deficiency. T2DM is believed to be attributable to the combined effect of genetic and environmental factors. Peroxisome proliferator-activated receptor gamma 2 (PPARγ2) is one of the main c...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-016-9765-6
更新日期：2017-02-01 00:00:00
abstract：:Three distinct malathion carboxylesterase (MCE) phenotypes have been identified among strains of Lucilia cuprina. The high-activity phenotype shows 1.6- and 33-fold more MCE specific activity than the intermediate- and low-activity phenotypes, respectively. Flies with high MCE activity are 1000-fold more resistant to ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00566064
更新日期：1994-12-01 00:00:00
abstract：:DNA damage repair elements accumulate at DNA damage sites to form ionizing radiation-induced foci (IRIF) for damage repair. IRIF, which represent direct evidence of DNA damage response activity, which are conveniently to be observed via immunofluorescence staining. Protein ubiquitination plays an important role in ini...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-015-9707-8
更新日期：2016-04-01 00:00:00
abstract：:Glycogen synthase, which catalyzes the synthesis of glycogen, is especially important for Old World (Pteropodidae) and New World (Phyllostomidae) fruit bats that ingest high-carbohydrate diets. Glycogen synthase 1, encoded by the Gys1 gene, is the glycogen synthase isozyme that functions in muscles. To determine wheth...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-014-9659-4
更新日期：2014-10-01 00:00:00
abstract：:We report a new enzyme xylose dehydrogenase, the structural locus for which is on chromosome 7 of the mouse, closely linked to Tam-1. Three alleles have been detected in both laboratory strains and wild populations. Two of these determine proteins differing in electrophoretic mobility and the third is a "null." This e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483970
更新日期：1982-08-01 00:00:00
abstract：:It is shown that the gene controlling the synthesis of the organ-specific S-esterase of Drosophila virilis ejaculatory bulbs is located on the second chromosome (at approximate position 192.1 +/- map units). The cells of the genital imaginal disks are determined for the synthesis of S-esterase 10-12 hr after the secon...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484728
更新日期：1978-08-01 00:00:00
abstract：:We extracted the yellow melanin (phaeomelanin), black melanin (eumelanin), and mixed type of melanin from dorsal hair of dominant yellow (Ay/a), non-agouti (a/a), and agouti (A/A) mice, respectively. Spectrophotometric and fluorescence spectrophotometric analysis demonstrated that the yellow melanin was qualitatively ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484724
更新日期：1978-08-01 00:00:00
abstract：:In order to clarify the genetic linkage groups of the Japanese brown frog Rana japonica, and compare them with those of other vertebrates, the inheritance of 10 enzyme and blood protein loci was examined in 267 offspring derived from 18 crosses using 10 males heterozygous at these loci. Most of the segregation tests e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554413
更新日期：1996-10-01 00:00:00
abstract：:UMPK 3 is a rare variant of the polymorphic enzyme of human red cells, uridine monophosphate kinase. This homozygote phenotype was detected among the Warao Indians of Venezuela. The UMPK 1 and UMPK 3 enzymes were partially purified following the method described by Tend et al. (1976). The biochemical and kinetic param...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484529
更新日期：1978-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00561468
更新日期：1988-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499142
更新日期：1983-04-01 00:00:00
abstract：:The nucleoside monophosphate kinases, adenylate kinase (AK), guanylate kinase (GUK), and uridine monophosphate kinase (UMPK), were studied electrophoretically and quantitatively in density gradient fractions of human red cells from normal adults which contain red cells of differing mean age. The enzymes were found to ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484542
更新日期：1978-12-01 00:00:00
abstract：:Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the ca...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1021950008544
更新日期：1997-06-01 00:00:00
abstract：:Active-site tryptic peptides were isolated from three genetic types of human serum cholinesterase. The active-site peptide was identified by labeling the active-site serine with [3H]diisopropylfluorophosphate. Peptides were purified by high-performance liquid chromatography. Amino acid composition and sequence analysi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499101
更新日期：1986-06-01 00:00:00
abstract：:ZFY-like genes have been observed in a variety of vertebrate species. Although originally implicated as the primary testis-determining gene in humans and other placental mammals, more recent evidence indicates a role(s) outside that of testis determination. In this study, DNA from five species of fish, Carasius auratu...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:Scirpophaga incertulas, commonly referred to as yellow stem borer, is a predominant pest of rice causing serious losses in its yield. Genetic variation among populations of Scirpophaga incertulas collected from 28 hotspot locations in India was examined using the randomly amplified polymorphic DNA-polymerase chain rea...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1002797219182
更新日期：2001-02-01 00:00:00
abstract：:An Italian organophosphate-resistant strain of Culex pipiens (Lucca) was found to be polymorphic for elevated and nonelevated esterases. Selection for high esterase activity produced a strain homozygous for elevated esterases A2 and B2. Selection for low activity produced a strain homozygous for nonelevated esterases,...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426878
更新日期：1993-12-01 00:00:00
abstract：:The locus for sorbitol dehydrogenase (SORD, E.C. 1.1.1.14) has been shown to segregate with hexosaminidase A and mannose phosphate isomerase in a series of human-Chinese hamster somatic cell hybrids. Cytogenetic analysis supports the assignment to chromosome 15 and further defines the gene locus to the region 15pter l...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484391
更新日期：1980-06-01 00:00:00
abstract：:The aryl hydrocarbon hydroxylase (Ah) locus that controls the induction of chemical carcinogen-metabolizing enzymes in mice has been found to be linked to a new restriction-fragment length polymorphism (RFLP). Only C57BL/6 and closely related inbred strains displayed a 7.6-kb HindIII restriction fragment, while all ot...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554549
更新日期：1987-06-01 00:00:00
abstract：:Two complementing loci in different linkage groups of the basidiomycete Ustilago violacea are involved in urease activity: a structural one (ure-1) and a second inferred to involve a permease (ure-2) locus. Two types of complementing mutations occur in the structural locus: null activity (ure-la) and obviously reduced...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484568
更新日期：1981-12-01 00:00:00
abstract：:Basal activity of submaxillary gland (SMG) renin is high in female mice that carry the Rnrs allele and is induced to higher levels by treatment with dihydrotestosterone (DHT). To determine whether the difference in basal activity between high (Rnrs/Rnrs) and low (Rnrb/Rnrb) strains is due to enhanced sensitivity of Rn...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484623
更新日期：1981-06-01 00:00:00
abstract：:Insulin-like growth factor (IGF-I) is an important growth factor in mammals, but the functions of the local muscle-specific isoform of insulin-like growth factor 1 (mIGF-1) to skeletal muscle development have rarely been reported. To determine the effect of pig mIGF-1 on body development and muscle deposition in vivo ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-9848-7
更新日期：2018-08-01 00:00:00
abstract：:The interspecies homology of dace supernatant (A2,AB,B2) and mitochondrial (C2) malate dehydrogenase isozymes has been established through cell fractionation and tissue distribution studies. Isolated supernatant malate dehydrogenase (s-MDH) isozymes show significant differences in Michaelis constants for oxaloacetate ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484591
更新日期：1980-08-01 00:00:00
abstract：:Genetic variations affecting the course of depressive symptoms in patients with coronary artery disease (CAD) have not yet been well studied. Therefore, we set out to investigate whether distinct haplotypes of the two insertion/deletion polymorphisms in the serotonin-transporter-linked polymorphic region (5-HTTLPR) an...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09967-w
更新日期：2020-08-01 00:00:00
abstract：:Apodemus peninsulae is a field mouse that inhabits the broad-leafed forests of temperate Eurasia. We examined the mitochondrial cytochrome b gene in 57 in dividuals of A. peninsulae from northeastern Asia, including Siberia, Primorye, Magadan region, Sakhalin, Hokkaido, and the Korean Peninsula. The genealogy of the m...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1015841424598
更新日期：2002-06-01 00:00:00
abstract：:UMP synthase was characterized biochemically in dairy cattle heterozygous for a deficiency of this enzyme. Both activities comprising this bifunctional enzyme are decreased, with OMP decarboxylase more affected than orotate phosphoribosyltransferase. Immunotitration of UMP synthase activity revealed the presence of th...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554349
更新日期：1987-08-01 00:00:00
abstract：:CCR5 is a seven-transmembrane G-protein-coupled receptor that binds the CC-chemokines including RANTES, eotaxin, MIP-1alpha and beta. CCR5 serves as an essential coreceptor for cell entry of R5 (macrophage-tropic, nonsyncytium-inducing) strains of HIV-1. To date, four deletions have been found in human and primate ccr...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：:Non-alcoholic fatty liver disease (NAFLD) have a high prevalence in humans in the past two decades. Here, we elucidated the functions of miR-30a-3p in the development of NAFLD and identified its potential targets. HepG-2 cells and NAFLD patients' blood samples were used in our study. Bioinformatics analysis as well as...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09971-0
更新日期：2020-10-01 00:00:00
abstract：:Prolamin mixtures were isolated from oats, rice, normal and high-lysine sorghum, two varieties of pearl millet, two strains of teosinte, and gamma grass and subjected to NH2-terminal amino acid sequence determinations. In each case (except for rice, whose prolamins apparently have blocked or unavailable NH2-terminal r...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00498931
更新日期：1982-12-01 00:00:00
abstract：:The goals of this study were to analyze the change in the global gene expression profile of exogenous human leukocyte antigen-G1 (HLA-G1) overexpressed in human embryonic stem (hES) cells and to explore the molecular mechanism by which the overexpression of HLA-G1 modifies immunologic pathways. Microarray and quantita...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-012-9522-4
更新日期：2012-10-01 00:00:00
