Chromosomal locations of ten isozyme loci in rice (Oryza sativa L.) through trisomic analysis.

Abstract:

:Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were determined through trisomic analysis. All 10 genes produced altered allozyme banding patterns in specific F1 trisomics. This served as the primary source of evidence for chromosome locations of Est-5, Icd-1, Acp-1, and Pgd-1. The locations of Amp-1, Amp-2, Amp-4, Pox-5, Got-1, and Cat-1 were further confirmed from segregation data in BC1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but agreed with the expected trisomic ratios. Triallelic heterozygotes were recovered for Amp-1 and Amp-2. On the basis of these data Got-1, Est-5, and Icd-1 were located to chromosome 1, Amp-1 to chromosome 2, Cat-1 and Pox-5 to chromosome 3, Acp-1 to chromosome 6, Amp-2 and Amp-4 to chromosome 8, and Pgd-1 to chromosome 11. Because Acp-2 and Pox-2 are known to be linked with Acp-1, they must also be on chromosome 6. The gene order and recombination values between isozyme loci on chromosomes 3, 6, 8, and 11 are presented.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Wu KS,Glaszmann JC,Khush GS

doi

10.1007/BF00561468

subject

Has Abstract

pub_date

1988-04-01 00:00:00

pages

303-20

issue

3-4

eissn

0006-2928

issn

1573-4927

journal_volume

26

pub_type

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