Abstract:
:UMP synthase was characterized biochemically in dairy cattle heterozygous for a deficiency of this enzyme. Both activities comprising this bifunctional enzyme are decreased, with OMP decarboxylase more affected than orotate phosphoribosyltransferase. Immunotitration of UMP synthase activity revealed the presence of the protein product of the mutant allele in the heterozygous animals. UMP synthases from normal and deficient cattle were not distinguished from one another by kinetic constants, responses to inhibitors, pH profiles, or thermal lability. It was concluded that the 50% reduction in enzyme activity in heterozygous cattle is the result of the presence of only half the normal level of catalytically active UMP synthase.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Harden KK,Robinson JLdoi
10.1007/BF00554349subject
Has Abstractpub_date
1987-08-01 00:00:00pages
465-75issue
7-8eissn
0006-2928issn
1573-4927journal_volume
25pub_type
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pub_type: 评论,杂志文章
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