Characterization of UMP synthase in dairy cattle heterozygous for a lethal recessive trait.

Abstract:

:UMP synthase was characterized biochemically in dairy cattle heterozygous for a deficiency of this enzyme. Both activities comprising this bifunctional enzyme are decreased, with OMP decarboxylase more affected than orotate phosphoribosyltransferase. Immunotitration of UMP synthase activity revealed the presence of the protein product of the mutant allele in the heterozygous animals. UMP synthases from normal and deficient cattle were not distinguished from one another by kinetic constants, responses to inhibitors, pH profiles, or thermal lability. It was concluded that the 50% reduction in enzyme activity in heterozygous cattle is the result of the presence of only half the normal level of catalytically active UMP synthase.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Harden KK,Robinson JL

doi

10.1007/BF00554349

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

465-75

issue

7-8

eissn

0006-2928

issn

1573-4927

journal_volume

25

pub_type

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