Abstract:
:A restriction map has been constructed for Anastrepha suspensa mitochondrial DNA. One HaeIII site was found to be polymorphic among individuals in highly inbred colonies and a feral population. Based on mapping information, the polymorphic site was determined to be in the ATPase 6 gene. Primers TK-J-3804 and C3-N-5460 amplified this region. The amplicon was cut by HaeIII in flies of one haplotype and not cut in flies of the other haplotype. From 30 to 43% of the individual flies studied had this additional HaeIlI site. After cloning of the approximately 5200 bp XbaI fragment, the two mitotypes were identified. A 988 base fragment, coding for the entire tRNA-Lys(AAG), tRNA-Asp(GAC), and ATPase 8genes, and a partial ATPase 6gene was sequenced Four silent mutations, including the one at the informative site were located. The HaeIII polymorphism and other sequence differences may prove useful as a diagnostic for identification of the origin of introduced fruit flies.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Heath M,Kuhn D,Schnell R,Olano Cdoi
10.1023/a:1019839102099subject
Has Abstractpub_date
2002-08-01 00:00:00pages
283-92issue
7-8eissn
0006-2928issn
1573-4927journal_volume
40pub_type
杂志文章abstract::Cleavage patterns of mitochondrial DNAs (mtDNAs) by 15 restriction endonucleases were analyzed for 10 swamp and 13 river types of domestic water buffaloes. Digestions with nine enzymes exhibited polymorphisms giving two or three kinds of cleavage patterns. Five mtDNA types were identified, three types in the swamp buf...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00554726
更新日期:1995-06-01 00:00:00
abstract::The genetic differentiation among the populations of the European abalone Haliotis tuberculata was investigated using different markers to better understand the evolutionary history and exchanges between populations. Three markers were used: mitochondrial cytochrome oxidase I (COI), the sperm lysin nuclear gene, and e...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-016-9778-1
更新日期:2017-04-01 00:00:00
abstract::We have isolated the Drosophila melanogaster locus coding for L-glutamate dehydrogenase (EC 1.4.1.3) by virtue of its similarity to the corresponding human gene. There is only one copy of this gene in the fruit fly genome, located on the right arm of chromosome 3 (95D1-4). The transcript includes at least one large in...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Genetic diversity and differentiation of nine populations of Ginkgo biloba L. (Ginkgoaceae)from China were evaluated using RAPD. Of 47 clear and repeatable RAPD bands, 46 were polymorphic (overall polymorphism = 97.9%). A ranged from 1.57 to 1.83 with a mean of 1.75. Mean He was 0.3159 (0.2429-0.3603). The Shannon ind...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/b:bigi.0000034431.15308.57
更新日期:2004-08-01 00:00:00
abstract::Studies on hybridization, inheritance, and population genetics of brown planthoppers that infest rice and weeds were undertaken using starch gel electrophoresis to determine whether the weed-infesting population represents a biological race or a species. F(1) and F(2) generations were produced by crosses between paren...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-009-9316-5
更新日期:2010-04-01 00:00:00
abstract::During a large-scale screen of a human fetal brain cDNA library, a full-length cDNA encoding a novel Rap2 interacting protein was isolated and sequenced. The cDNA is 3397bp long and has a predicted open reading frame encoding a protein of 329 aa. The predicted protein shows high homology to mouse and human RPIP8, and ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1020918411532
更新日期:2003-02-01 00:00:00
abstract::DNA polymorphisms in the psi beta 1-beta-globin gene region in nine Asian macaques (Macaca fuscata, M. mulatta, M. nemestrina, M. cyclopis, M. fascicularis, M. arctoides, M. radiata, M. maura, and M. assamensis) were examined using several restriction endonucleasese and the human psi beta 1, delta IVS2, and beta IVS2 ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02401811
更新日期:1991-04-01 00:00:00
abstract::A new variant of hereditary hemolytic anemia in a family due to high adenosine triphosphate (ATP) is reported. The increase in ATP levels varied from 83 to 105% in the family members. Low 2,3-diphosphoglycerate levels and low 2,3-diphosphoglyceromutase activity were observed in three family members, with normal glucos...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504264
更新日期:1981-10-01 00:00:00
abstract::Techniques are presented to detect 23 isozyme loci in the long-lived perennial plant, ponderosa pine. Meiotically derived megagametophyte from seeds is used to examine directly the segregation of allelic variants. Approximately seven seeds were initially examined for 12 enzymes from each of 47 trees from ten stands th...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00498965
更新日期:1979-04-01 00:00:00
abstract::Male and female wild Norway rats (Rattus norvegicus Erxleben) and male and female albino outbred rats (Ipf:RIZ) were crossbred. These animals were the control, noninbred group (0% inbred). By systematic full-sib mating, two experimental groups (50 and 91% of inbred) were raised. Half of each group (both males and fema...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00569326
更新日期:1992-08-01 00:00:00
abstract::Using polyacrylamide gel isoelectric focusing followed by immunoblotting with anti-human apolipoprotein E (APO E) antibody, the genetic polymorphism of APO E was determined from desialylated plasma of 554 unrelated adults of four European sheep (Suffolk, Corriedale, Cheviot, and Finnish Landrace) and five Asian local ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1018753714783
更新日期:1998-12-01 00:00:00
abstract::Mitochondrial DNA (mtDNA) hypervariable segment I sequences (HVSI, 471 bp) of the control region and partial cytochrome b sequences (Cytb, 403 bp) were analyzed in three tentative species of the genus Mystacoleucus in China (M. chilopterus, M. marginatus, and M. lepturus). Not more than two mutations were found in bot...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/b:bigi.0000006031.20967.08
更新日期:2003-10-01 00:00:00
abstract::Oral cancer is a multifactorial disease process and involves complex interactions between gene to gene and gene to environmental factors. Interleukin 8 (IL-8), a pro-inflammatory cytokine, having angiogenic activity with elevated expression in tumor cells, is reported to play an essential role in oral cancer developme...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-015-9704-y
更新日期:2016-02-01 00:00:00
abstract::Two alleles are encoded at the malate dehydrogenase locus in largemouth bass, Micropterus salmoides. Populations in the extreme northern areas of the range of this fish are fixed or nearly fixed for the B1 allele, whereas populations in Florida are fixed for the alternative allele, B2. The MDH-B1B1 and MDH-B2B2 alleli...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00488466
更新日期:1983-12-01 00:00:00
abstract::Two complementing loci in different linkage groups of the basidiomycete Ustilago violacea are involved in urease activity: a structural one (ure-1) and a second inferred to involve a permease (ure-2) locus. Two types of complementing mutations occur in the structural locus: null activity (ure-la) and obviously reduced...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484568
更新日期:1981-12-01 00:00:00
abstract::The enzyme UDPglucose:flavonol 3-O-glucosyltransferase is shown to be under the coordinate control of three genes involved in anthocyanin biosynthesis in the aleurone of maize: C, R, and BZ. Of the three, BZ appears to be the structural gene. Data presented here (dosage comparisons, induction in the mutant c-p, and ef...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00520194
更新日期:1977-06-01 00:00:00
abstract::The domesticated turkey, Meleagris gallopavo, is believed to be a single breed with several varieties whose relatedness and origins remain poorly understood. Using the mitochondrial genome sequence (GenBank accession no. EF153719) that our group first reported, we investigated the relationships among 15 of the most wi...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-015-9668-y
更新日期:2015-04-01 00:00:00
abstract::Single-chain variable fragment (scFv) is one of the most common antibody forms. This report describes the expression of the scFv gene as a soluble protein in Origami DE3 cytoplasm. The purified scFv recognized the epidermal growth factor receptor (EGFRvIII) on the surface of MCF-7 cells. The scFv protein was purified ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9506-4
更新日期:2012-08-01 00:00:00
abstract::CCR5 is a seven-transmembrane G-protein-coupled receptor that binds the CC-chemokines including RANTES, eotaxin, MIP-1alpha and beta. CCR5 serves as an essential coreceptor for cell entry of R5 (macrophage-tropic, nonsyncytium-inducing) strains of HIV-1. To date, four deletions have been found in human and primate ccr...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::Two types of alleles exist in the human alcohol dehydrogenase-2 (ADH2) locus. The usual ADH12 allele is common in Caucasians, while the atypical ADH22 allele is predominant in Orientals. The ADH22 produces the beta 2 subunit, which is catalytically far more active than the beta 1 subunit produced by the ADH12 gene. Th...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02399419
更新日期:1988-08-01 00:00:00
abstract::Larval serum protein-1 (LSP-1) and LSP-2 are the major proteins of Drosophila larval serum. The amount of LSP-1 synthesized is strictly proportional to the number of LSP-1 genes present within the range 1-10. The normal number in female flies is 6. Flies with extreme amounts of LSP-1 were, by our criteria, as fit as t...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499473
更新日期:1984-10-01 00:00:00
abstract::The secretogranin II (SCG2) gene is associated with the synthesis and secretion of follicle-stimulating hormone and luteinizing hormone. In the present study, we have determined the complete cDNA sequence of pig SCG2, which was submitted to GenBank with accession no. AY870646. Its complete open reading frame of 1,851 ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-008-9153-y
更新日期:2008-06-01 00:00:00
abstract::Myogenic differentiation 1 (MyoD1) genes belong to the MyoD gene family and play key roles in growth and muscle development. This study was designed to investigate the effects of variants in the MyoD1 gene on duck growth and carcass traits. Three duck populations (Cherry Valley, Jingjiang, and Muscovy) were sampled, t...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9530-4
更新日期:2012-12-01 00:00:00
abstract::A novel cDNA fragment was identified from a human fetal brain cDNA library by using the coding sequence of human BRI3 gene (Accession No. NM015379) as bait in a yeast two-hybrid screening. Then by 5'-RACE (rapid amplification of cDNA end) and electronic hybridization, we obtained a 1.9 kb contig which consists of a no...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1013807518791
更新日期:2001-12-01 00:00:00
abstract::The nuclear factor of activated T lymphocytes (NFATc1) plays a critical role during valvular and septal development. Genetic variants may influence the biological function of the protein and thus play a role in susceptibility to valvuloseptal defects. Tandem repeat polymorphisms and a common nonsynonymous polymorphism...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-011-9434-8
更新日期:2011-10-01 00:00:00
abstract::To determine the prevalence of G6PD deficiency and a Mediterranean mutation among males in southeastern Iran, we studied 1,097 Sistani and Balouch schoolboys. A questionnaire was used to collect demographic data and a history of malaria infection; blood samples were evaluated for G6PD deficiency and the G6PD Mediterra...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9548-7
更新日期:2013-02-01 00:00:00
abstract::UMPK 3 is a rare variant of the polymorphic enzyme of human red cells, uridine monophosphate kinase. This homozygote phenotype was detected among the Warao Indians of Venezuela. The UMPK 1 and UMPK 3 enzymes were partially purified following the method described by Tend et al. (1976). The biochemical and kinetic param...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484529
更新日期:1978-12-01 00:00:00
abstract::Skeletal muscle tissues from many species of salmonid fish are known to exhibit a set of three of five isozymes for A4-type lactate dehydrogenase (L-lactate: NAD oxidoreductase, E.C. 1.1.1.27), but the genetic basis for this isozyme system has not previously been assessed. This isozyme system was purified to homogenei...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484099
更新日期:1977-08-01 00:00:00
abstract::The solubilized proteins of liver and brain from mice of two inbred strains (C57BL/6J and DBA/2J) and their hybrids were subfractionated by heparin Sepharose (H-S) CL-6B affinity chromatography. The H-S binding and nonbinding proteins were separated by two-dimensional electrophoresis. The protein patterns obtained wer...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00554529
更新日期:1986-12-01 00:00:00
abstract::A new polymorphism, Pc, has been identified in human saliva. Two proteins, Pc 1 and Pc 2, are determined by alleles Pc1 and Pc2, respectively, which show autosomal codominant inheritance. No null phenotype has been encountered in 225 randomly collected salivas. The frequencies of the two alleles differ in the Black an...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499111
更新日期:1985-02-01 00:00:00