Abstract：
:Non-alcoholic fatty liver disease (NAFLD) have a high prevalence in humans in the past two decades. Here, we elucidated the functions of miR-30a-3p in the development of NAFLD and identified its potential targets. HepG-2 cells and NAFLD patients' blood samples were used in our study. Bioinformatics analysis as well as luciferase reporter assays were employed to distinguish peroxisome proliferator-activated receptor alpha (PPAR-α) as a target gene. Western blotting showed the expressions of lipid metabolic proteins and the target gene PPAR-α. Oil red O staining and triglyceride activity tested the fatty deposits after treatment with miR-30a-3p. miR-30a-3p was substantially up-regulated in NAFLD. Bioinformatics analyses showed that PPAR-α was a possible target of miR-30a-3p, linked with signaling pathways in NAFLD. PPAR-α as a novel target of miR-30a-3p, and suppression of its levels. The lipid metabolic-related proteins ACC, p-GSK-3β and FASN were up-regulated after transfecting with miR-30a-3p mimic, but the proteins CPT1, p-AMPK and UCP2 were down-regulated. miR-30a-3p inhibitor could diminish the protein manifestation of ACC, p-GSK-3β and FASN; and augment the protein manifestation of CPT1, p-AMPK and UCP2. On the contrary, overexpression of miR-30a-3p had adverse impacts on the performance of hepatocellular lipid accumulation and Triglyceride (TG) activity. Co-treatment with miR-30a-3p mimic and overexpression PPAR-α could revise the hepatic steatosis and the TG level induced by fat milk. Our findings suggest that miR-30a-3p/PPAR-α may be developed as a potential agent in NAFLD, which is enough to attenuate triglyceride accumulation and hepatic steatosis.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Wang DR,Wang B,Yang M,Liu ZL,Sun J,Wang Y,Sun H,Xie LJdoi
10.1007/s10528-020-09971-0subject
Has Abstractpub_date
2020-10-01 00:00:00pages
691-704issue
5eissn
0006-2928issn
1573-4927pii
10.1007/s10528-020-09971-0journal_volume
58pub_type
杂志文章abstract：:Insulin-like growth factor (IGF-I) is an important growth factor in mammals, but the functions of the local muscle-specific isoform of insulin-like growth factor 1 (mIGF-1) to skeletal muscle development have rarely been reported. To determine the effect of pig mIGF-1 on body development and muscle deposition in vivo ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-9848-7
更新日期：2018-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/BF00499943
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journal_title：Biochemical genetics
pub_type： 杂志文章
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journal_title：Biochemical genetics
pub_type： 杂志文章
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00520194
更新日期：1977-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00504335
更新日期：1986-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00499474
更新日期：1984-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484525
更新日期：1978-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10528-008-9153-y
更新日期：2008-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485847
更新日期：1984-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499296
更新日期：1984-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554618
更新日期：1994-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484739
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499142
更新日期：1983-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499114
更新日期：1985-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554529
更新日期：1986-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485784
更新日期：1975-08-01 00:00:00
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pub_type： 杂志文章
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-012-9530-4
更新日期：2012-12-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2015-10-01 00:00:00
