Abstract：
:Non-alcoholic fatty liver disease is one of the main causes of chronic liver disease and therefore is currently considered a major public health problem. Sirtuin 1 (SIRT1) is an NAD-dependent deacetylase enzyme that contributes in the regulation of metabolic processes and protects against lipid accumulation in hepatocytes. Its expression is potentially regulated by microRNAs which attach to the 3' untranslated region (3'-UTR) of their target mRNA. HepG2 cells were incubated by glucose to induce lipid accumulation and were subsequently transfected with mir-23b mimic and inhibitor. Real-time PCR was used for measuring the expression of mir-23b and SIRT1 mRNA. Cell survival assay and intracellular triglyceride measurement were performed using colorimetric methods. Determination of SIRT1 protein level and activity were done by western blot and fluorometric analysis, respectively. The interaction of miR-23b with 3'-UTR of SIRT1 mRNA was confirmed by dual luciferase. miR-23b mimic inhibited gene and protein expression of SIRT1, while the inhibitor of miR-23b significantly elevated the expression levels of SIRT1 mRNA and protein. The results showed that the 3'-UTR of SIRT1 mRNA is a direct target for miR-23b. The intracellular triglyceride level was increased following the inhibition of SIRT1 in transfected HepG2 cell by miR-23b mimic. Cell viability was decreased in response to miR-23b upregulation compared to control cells. miR-23b reduces the expression and activity of SIRT1 and therefore may be a causative factor in the enhancement of lipid accumulation in HepG2 cells.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Borji M,Nourbakhsh M,Shafiee SM,Owji AA,Abdolvahabi Z,Hesari Z,Ilbeigi D,Seiri P,Yousefi Zdoi
10.1007/s10528-019-09905-5subject
Has Abstractpub_date
2019-08-01 00:00:00pages
507-521issue
4eissn
0006-2928issn
1573-4927pii
10.1007/s10528-019-09905-5journal_volume
57pub_type
杂志文章abstract：:Salvia miltiorrhiza is a well-known medicinal plant and functional food in Asia. The range of quality among its germplasms is irregular, which inevitably affects its health and economic benefits. We established a system comprising phenotypic characteristics, ISSR markers, and active constituents to facilitate the comp...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9600-2
更新日期：2013-10-01 00:00:00
abstract：:The alpha-amylase in Drosophila melanogaster is a highly polymorphic enzyme, at both the allozyme level and the specific activity level. This enzyme changes its specific activity drastically depending on both food conditions and developmental stages, and it has been suggested that the ability to change its activity de...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1018715528413
更新日期：1999-12-01 00:00:00
abstract：:Insulin-like growth factor (IGF-I) is an important growth factor in mammals, but the functions of the local muscle-specific isoform of insulin-like growth factor 1 (mIGF-1) to skeletal muscle development have rarely been reported. To determine the effect of pig mIGF-1 on body development and muscle deposition in vivo ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-9848-7
更新日期：2018-08-01 00:00:00
abstract：:The present study intends to survey the genetic variability of an endangered semiferal Portuguese native pony breed, the Garrano. Thirteen microsatellite markers were examined in 277 animals born in 1998, belonging to eight subpopulations corresponding to eight northern Portuguese geographic regions. Mean heterozygosi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-005-6775-1
更新日期：2005-08-01 00:00:00
abstract：:Larval serum protein-1 (LSP-1) and LSP-2 are the major proteins of Drosophila larval serum. The amount of LSP-1 synthesized is strictly proportional to the number of LSP-1 genes present within the range 1-10. The normal number in female flies is 6. Flies with extreme amounts of LSP-1 were, by our criteria, as fit as t...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499473
更新日期：1984-10-01 00:00:00
abstract：:Ubiquitin-specific protease 9, Y-linked (USP9Y), is a protein encoded by the Y chromosome. Its precise function in the cell is unknown, although a role in the regulation of protein turnover has been postulated. Nonetheless, mutations in this gene could result in the over- or under-abundance of proteins involved in the...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-007-9139-1
更新日期：2008-04-01 00:00:00
abstract：:Liver homogenate-supernatants from most Japanese exhibit an "atypical" pH optimum for ethanol oxidation at pH 8.8 instead of 10.5, the "typical" pH-activity optimum. It has been proposed that atypical livers contain alcohol dehydrogenase isozymes with beta 2 subunits while typical livers contain isozymes with beta 1 s...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499296
更新日期：1984-02-01 00:00:00
abstract：:The acidic proline-rich proteins (Pr) showing genetic polymorphism were purified from human parotid salivas by gel filtration and ion exchange chromatography. Molecular weight determinations, amino acid composition analyses, and polypeptide mapping experiments indicate that the Pr 3 protein is a fragment of the Pr 1 p...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504345
更新日期：1979-12-01 00:00:00
abstract：:Oral cancer is a multifactorial disease process and involves complex interactions between gene to gene and gene to environmental factors. Interleukin 8 (IL-8), a pro-inflammatory cytokine, having angiogenic activity with elevated expression in tumor cells, is reported to play an essential role in oral cancer developme...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-015-9704-y
更新日期：2016-02-01 00:00:00
abstract：:Upland rice landraces from different villages of Mizoram, Northeast India were analyzed for seed morphology, amylose content, aromatic characteristic, seed storage protein profiling and genetic diversity. Results revealed variation in grain length, width, weight and shape. Protein profiling showed polypeptide bands ra...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-09901-1
更新日期：2019-06-01 00:00:00
abstract：:We report a new enzyme xylose dehydrogenase, the structural locus for which is on chromosome 7 of the mouse, closely linked to Tam-1. Three alleles have been detected in both laboratory strains and wild populations. Two of these determine proteins differing in electrophoretic mobility and the third is a "null." This e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483970
更新日期：1982-08-01 00:00:00
abstract：:This study deals with the characterization of 10 populations of M. musculus from different geographical locations in India. The genetics of Indian wild mice has been completely obscure and this is the first report on allozyme variations in the natural population. We have used a set of 24 biochemical genetic markers to...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1018781026170
更新日期：1998-08-01 00:00:00
abstract：:The genetic differentiation among the populations of the European abalone Haliotis tuberculata was investigated using different markers to better understand the evolutionary history and exchanges between populations. Three markers were used: mitochondrial cytochrome oxidase I (COI), the sperm lysin nuclear gene, and e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-016-9778-1
更新日期：2017-04-01 00:00:00
abstract：:Investigations of an euploid series of castor bean, Ricinus communis L., Consisting of haploid, diploid, and tetraploid individuals, was performed to determine the value of such a series in studying the biochemical consequences of genome multiplication. The effects of euploidization of the nuclear genome on the biosyn...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504367
更新日期：1980-02-01 00:00:00
abstract：:The locus for sorbitol dehydrogenase (SORD, E.C. 1.1.1.14) has been shown to segregate with hexosaminidase A and mannose phosphate isomerase in a series of human-Chinese hamster somatic cell hybrids. Cytogenetic analysis supports the assignment to chromosome 15 and further defines the gene locus to the region 15pter l...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484391
更新日期：1980-06-01 00:00:00
abstract：:In order to clarify the genetic linkage groups of the Japanese brown frog Rana japonica, and compare them with those of other vertebrates, the inheritance of 10 enzyme and blood protein loci was examined in 267 offspring derived from 18 crosses using 10 males heterozygous at these loci. Most of the segregation tests e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554413
更新日期：1996-10-01 00:00:00
abstract：:The genetic diversity of the Turkish native chicken breeds Denizli and Gerze was evaluated with 10 microsatellite markers. We genotyped a total of 125 individuals from five subpopulations. Among loci, the mean number of alleles was 7.5, expected heterozygosity (H (e)) was 0.665, PIC value was 0.610, and Wright's fixat...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-008-9164-8
更新日期：2008-08-01 00:00:00
abstract：:Differences in Zn-induced levels of hepatic metallothionein (MT) in inbred strains of the mouse are described. Three low-producing strains, C57 BL/6, C57BL/10, and NIH, are identified, while C3H and CBA display the highest levels of hepatic MT following Zn treatment. These interstrain differences affect not only the l...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02401801
更新日期：1989-04-01 00:00:00
abstract：:Allelic frequencies at five polymorphic loci were determined in seven European and six Afrotropical populations of Drosophila melanogaster. African populations, which may be considered as ancestral for the species, showed a greater genetic diversity as measured by the number of alleles found. Within each geographic gr...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483971
更新日期：1982-08-01 00:00:00
abstract：:Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the structure of hair folli...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-019-09929-x
更新日期：2019-10-01 00:00:00
abstract：:The variable numbers of tandem repeats (VNTR) locus D1S80, located on chromosome 1 (1p35-36), has a repeat unit 16 bp in length, and different numbers of these repeat units have been observed for populations of different origins and ethnicity. We used a molecular identification method based on capillary electrophoresi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-009-9250-6
更新日期：2009-08-01 00:00:00
abstract：:A new hemoglobin variant found in a mother and her child was characterized by column chromatography of the tryptic hydrolysate of the aminoethylated, glycinamidated beta-chain, followed by chymotryptic digestion of the abnormal beta T-9 peptide and amino acid analyses. It was shown to be alpha2beta2 73(E17)Asp replace...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485784
更新日期：1975-08-01 00:00:00
abstract：:The aryl hydrocarbon hydroxylase (Ah) locus that controls the induction of chemical carcinogen-metabolizing enzymes in mice has been found to be linked to a new restriction-fragment length polymorphism (RFLP). Only C57BL/6 and closely related inbred strains displayed a 7.6-kb HindIII restriction fragment, while all ot...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554549
更新日期：1987-06-01 00:00:00
abstract：:The aldox-2 locus in Drosophila melanogaster has been shown to affect differentially three molybdoenzymes, aldehyde oxidase, pyridoxal oxidase, and xanthine dehydrogenase. These effects are most obvious at times surrounding the pupal-adult boundary, when the normal organism accumulates large amounts of these enzymes i...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499002
更新日期：1986-10-01 00:00:00
abstract：:An Italian organophosphate-resistant strain of Culex pipiens (Lucca) was found to be polymorphic for elevated and nonelevated esterases. Selection for high esterase activity produced a strain homozygous for elevated esterases A2 and B2. Selection for low activity produced a strain homozygous for nonelevated esterases,...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426878
更新日期：1993-12-01 00:00:00
abstract：:Studies have investigated the association between angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and cardiometabolic risk factors (CMRFs), however with varying results, which could be due to ethnicity differences. Therefore, the present study was conducted among Bhil tribal population (...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-9845-x
更新日期：2018-08-01 00:00:00
abstract：:Ade-H and ade-I are two auxotrophic mutants of Chinese hamster ovary (CHO-K1) cells which specifically require adenine as the purine source to grow. The enzymatic defects of these mutants were examined in cell-free extracts. It was found that ade-H did not have any detectable adenylosuccinate synthetase activity and a...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484561
更新日期：1977-02-01 00:00:00
abstract：:Starch gel electrophoresis and histochemical staining with L-leucylglycyl-glycine revealed genetic polymorphism in peptidase B in cattle erythrocytes. Genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, PepB1 and PepB2. A...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:This study aims to compare the expression of P2X receptor subtype mRNA in different arterial tissues of rats. After the rats were sacrificed, the internal carotid, pulmonary, thoracic aorta, mesenteric and caudal arteries were dissected out. Then, the P2X receptor mRNA expression in different blood vessels was detecte...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09968-9
更新日期：2020-10-01 00:00:00
abstract：:Two types of alleles exist in the human alcohol dehydrogenase-2 (ADH2) locus. The usual ADH12 allele is common in Caucasians, while the atypical ADH22 allele is predominant in Orientals. The ADH22 produces the beta 2 subunit, which is catalytically far more active than the beta 1 subunit produced by the ADH12 gene. Th...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02399419
更新日期：1988-08-01 00:00:00
