Abstract：
:This study aims to compare the expression of P2X receptor subtype mRNA in different arterial tissues of rats. After the rats were sacrificed, the internal carotid, pulmonary, thoracic aorta, mesenteric and caudal arteries were dissected out. Then, the P2X receptor mRNA expression in different blood vessels was detected by reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative polymerase chain reaction. The P2X1, P2X4 and P2X7 receptor mRNA amplification products revealed specific bands of the same size as the amplified target fragment in their respective lanes, while the P2X2, P2X3, P2X5 and P2X6 receptor mRNA amplification products did not reveal significant specific bands in their respective lanes by RT-PCR. Based on the P2X1 receptor mRNA expression of the mesenteric artery, there were no significant differences in the internal carotid, pulmonary and thoracic aorta (0.64 ± 0.07, 0.17 ± 0.11 and 1.49 ± 0.65, respectively). However, the P2X1 receptor mRNA expression level in the caudal artery significantly increased (11.06 ± 1.99, P < 0.01). Furthermore, there was no difference in P2X4 receptor mRNA expression among these five blood vessels (P > 0.05). The P2X7 receptor mRNA expression level was significantly different: pulmonary artery < tail artery = thoracic aorta < internal carotid artery < mesenteric artery. The relative P2X1 receptor mRNA expression in the caudal artery was observed to be elevated when compared to that of the internal carotid, pulmonary and thoracic aorta as well as the mesenteric arteries. The P2X7 receptor mRNA expression level is pulmonary artery < caudal artery = thoracic aorta < internal carotid artery < mesenteric artery. P2X4 receptor mRNA expression was not significantly different among these five blood vessels.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Li LZ,Yue LH,Zhang ZM,Zhao J,Ren LM,Wang HJ,Li Ldoi
10.1007/s10528-020-09968-9subject
Has Abstractpub_date
2020-10-01 00:00:00pages
677-690issue
5eissn
0006-2928issn
1573-4927pii
10.1007/s10528-020-09968-9journal_volume
58pub_type
杂志文章abstract：:beta-Glucuronidase (GUS) has become an important enzyme model for the genetic study of molecular disease, enzyme realization, and therapy, and for the biogenesis and function of the lysosome and lysosomal enzymes. The genetics of human beta-glucuronidase was investigated utilizing 188 primary man-mouse and man-chinese...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484467
更新日期：1977-04-01 00:00:00
abstract：:The genetic linkage relationships of the human glycosphingolipid beta-galactosidases were determined using human--mouse somatic cell hybrids. A new method was devised for the estimation of human galactosylceramide, lactosylceramide, and GMI-ganglioside beta-galactosidase activities in the presence of their mouse count...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484498
更新日期：1977-12-01 00:00:00
abstract：:The variable numbers of tandem repeats (VNTR) locus D1S80, located on chromosome 1 (1p35-36), has a repeat unit 16 bp in length, and different numbers of these repeat units have been observed for populations of different origins and ethnicity. We used a molecular identification method based on capillary electrophoresi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-009-9250-6
更新日期：2009-08-01 00:00:00
abstract：:Restriction endonuclease analyses of DNA from one Black G gamma A gamma-HPFH homozygote and four Black and one Indian G gamma A gamma-HPFH heterozygotes have identified three different HPFH types which are the result of large deletions including the delta and beta genes. Two of the types are comparable to those charac...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499284
更新日期：1984-02-01 00:00:00
abstract：:Hippophae rhamnoides ssp. sinensis is endemic to China, and it is a dioecious, outcrossing plant. Although many studies have been undertaken mainly on its agricultural, nutritional, medical, and ornamental value, little is known about its population genetics. This study uses random amplified polymorphic DNA to investi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-006-9025-2
更新日期：2006-06-01 00:00:00
abstract：:To determine the genetic structure and variation of Van cats and some other cats, seven enzyme loci were examined using horizontal starch gel electrophoresis. ME bands were observed for the first time in cats. For the enzyme loci CA ( 1 ), SOD, GPI, and GOT, neither the individual Van cats nor the specimens of other c...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-011-9426-8
更新日期：2011-08-01 00:00:00
abstract：:Soluble starch synthase and branching enzyme were purified from 18-day-old cotyledons of the smooth-seeded pea cultivar Alaska (RR) and wrinkled-seeded pea cultivar Progress #9 (rr) by DEAE-cellulose chromatography. Two coeluting peaks of primed and citrate-stimulated starch synthase activity and a major and minor pea...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484061
更新日期：1982-10-01 00:00:00
abstract：:To verify the quality of triploid varieties of Camellia tea species at the secondary metabolite level, we tested caffeine and catechin profiles of 97 F(1) segregating progenies in two breeding populations with a common tetraploid parent and diploid parents of two geographic and varietal origins. Catechin and caffeine ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9569-x
更新日期：2013-06-01 00:00:00
abstract：:A genetic locus controlling the electrophoretic mobility of a methylglyoxal dehydrogenase (EC 1.2.1.23) in the rat is described. The locus, designated Mgd1, is expressed in liver and kidney. Inbred rat strains have fixed either allele Mgd1a or allele Mgd1b. Codominant expression is observed in heterozygotes, providing...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554618
更新日期：1994-06-01 00:00:00
abstract：:This haplotype-based case-control study investigated whether the aldosterone synthase gene (CYP11B2) might be implicated in the pathogenesis of essential hypertension in Yi (226 individuals) and Hani (296 individuals) minorities of China. Four tag SNPs (rs4536, rs4545, rs3097, and rs3802230) and the K173R polymorphism...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9393-5
更新日期：2011-02-01 00:00:00
abstract：:Luo [Biochem. Genet. 43:223-227] concluded, "The mutation ALDH2(() 487Lys allele is not deleterious but is of great benefit to human health." This statement is easily subject to misinterpretation and needs to be clarified. Their results actually show there is a pleiotropic effect associated with the mutation ALDH2(() ...
journal_title：Biochemical genetics
pub_type： 评论,杂志文章
doi：10.1007/s10528-006-9041-2
更新日期：2006-10-01 00:00:00
abstract：:Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patter...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9607-8
更新日期：2013-10-01 00:00:00
abstract：:A survey of 37 inbred strains for erythrocyte purine nucleoside phosphorylase activity showed a greater than threefold range. Six of these strains had significantly greater activity than the others, and all of the high-activity strains had the Np-2 electrophoretic band. The high-purine nucleoside phosphorylase activit...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499142
更新日期：1983-04-01 00:00:00
abstract：:Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six bre...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-008-9152-z
更新日期：2008-06-01 00:00:00
abstract：:The molecular nature of chicken fetal antigen (CFA) and quail fetal antigen (QFA) was studied on embryonic red blood cells (RBCs) of the chicken, the Japanese quail, and the quail-chicken hybrid. Specific immunoprecipitation of radiolabeled membrane proteins followed by electrophoretic separation and autoradiography w...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499476
更新日期：1984-10-01 00:00:00
abstract：:The acidic proline-rich proteins (Pr) showing genetic polymorphism were purified from human parotid salivas by gel filtration and ion exchange chromatography. Molecular weight determinations, amino acid composition analyses, and polypeptide mapping experiments indicate that the Pr 3 protein is a fragment of the Pr 1 p...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504345
更新日期：1979-12-01 00:00:00
abstract：:Genetic variation at three dipeptidase loci (Dip-A, Dip-B, and Dip-C) in Drosophila simulans was analyzed by starch gel electrophoresis. Dip-A was found to be polymorphic in four populations, while Dip-B and Dip-C were found to be polymorphic in one. The numbers of different alleles found at each respective locus were...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00486138
更新日期：1981-02-01 00:00:00
abstract：:We have isolated the Drosophila melanogaster locus coding for L-glutamate dehydrogenase (EC 1.4.1.3) by virtue of its similarity to the corresponding human gene. There is only one copy of this gene in the fruit fly genome, located on the right arm of chromosome 3 (95D1-4). The transcript includes at least one large in...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:The rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9584-y
更新日期：2013-08-01 00:00:00
abstract：:The extent of naturally occurring variations of enzyme locus expression was determined for three tissues (liver, muscle, and eye) in two species of sunfish (Centrarchidae), the green sunfish (Lepomis cyanellus) and the redear sunfish (L. microlophus). The genetic basis for species differences in tissue enzyme specific...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499484
更新日期：1984-10-01 00:00:00
abstract：:Chronic myeloid leukemia (CML) is a hematopoietic stem cell malignancy characterized by the expression of the BCR-ABL1 fusion gene with different chimeric transcripts. Despite the crucial impact of constitutively active tyrosine kinase in CML pathogenesis, aberrant DNA methylation of certain genes plays an important r...
journal_title：Biochemical genetics
pub_type： 杂志文章,评审
doi：10.1007/s10528-018-9841-1
更新日期：2018-06-01 00:00:00
abstract：:Non-alcoholic fatty liver disease is one of the main causes of chronic liver disease and therefore is currently considered a major public health problem. Sirtuin 1 (SIRT1) is an NAD-dependent deacetylase enzyme that contributes in the regulation of metabolic processes and protects against lipid accumulation in hepatoc...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-019-09905-5
更新日期：2019-08-01 00:00:00
abstract：:An Italian organophosphate-resistant strain of Culex pipiens (Lucca) was found to be polymorphic for elevated and nonelevated esterases. Selection for high esterase activity produced a strain homozygous for elevated esterases A2 and B2. Selection for low activity produced a strain homozygous for nonelevated esterases,...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426878
更新日期：1993-12-01 00:00:00
abstract：:A slow-migrating beta-esterase (S-esterase) is described which has been detected in Drosophila montana, Drosophila imeretensis, and some stocks of Drosophila virilis when mixtures of alpha- and beta-naphthyl acetate are used as substrates in histochemical reactions after electrophoresis. Sexual dimorphism for S-estera...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484882
更新日期：1976-02-01 00:00:00
abstract：:Among the European fauna, the Sardinian hare (Lepus sp.) is peculiar in that it differs from all other hares inhabiting the continent. Here, we report on the variation of a 461 bp sequence of hypervariable domain 1 of the mitochondrial control region, examined in 42 hares collected throughout Sardinia and compared to ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-007-9076-z
更新日期：2007-04-01 00:00:00
abstract：:Two species of the genus Taricha are widely distributed. T. granulosa ranges from southern Alaska to central California. T. torosa is comprised of two described subspecies, T. t. torosa, which occupies much of the coast ranges of California, and T. t. sierrae, which inhabits the western slopes of the Sierra Nevada Mou...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485835
更新日期：1976-08-01 00:00:00
abstract：:A survey of laboratory strains of Anopheles stephensi for nonspecific esterases by polyacrylamide gel electrophoresis revealed 10 zones of esterase activity. In 3 of the 10 zones, three electromorphs were observed. Genetic analysis revealed that these three zones are controlled by three loci, viz., Est-3, Est-4, and E...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484518
更新日期：1984-06-01 00:00:00
abstract：:DNA repair systems are responsible for maintaining the integrity of the genome and have a critical role in protecting against mutations that can lead to cancer. DNA repair gene products of ERCC1 and ERCC2/XPD are involved in the nucleotide excision repair pathway. The allele frequencies of the polymorphisms ERCC1 G190...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-005-8170-3
更新日期：2005-10-01 00:00:00
abstract：:A variant of fetal hemoglobin (Hb F-Malta-I) has been detected and quantitated in adult blood with a sensitive radioimmunoassay employing monospecific antisera. The concentration of Hb-F-Malta-I was 0.002-0.05%, with an average value of 0.011%. The ratio of Hb F-Malta-I/Hb F in adults was about 4.8%, compared to a rat...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483988
更新日期：1977-10-01 00:00:00
abstract：:The segregation of human lysosomal arylsulfatase A (ARS-A) has been evaluated in 50 primary hybrid clones derived from four separate fusions involving WBCs from two unrelated individuals and three hamster cell lines. ARS-A was expressed in the hybrids as a dimeric molecule of very similar or identical subunits. The ex...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504344
更新日期：1979-12-01 00:00:00
